Browse > Article
http://dx.doi.org/10.5338/KJEA.2018.37.2.18

Intestine Bacterial Microbiota of Asian Hornet (Vespa Velutina Nigrithorax) and Honey Bee  

Kim, Euyeon (Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Chonnam National University)
Seo, Jeongwon (Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Chonnam National University)
Yang, So Hee (Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Chonnam National University)
Kim, In-Seon (Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Chonnam National University)
Koo, Yeonjong (Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Chonnam National University)
Publication Information
Korean Journal of Environmental Agriculture / v.37, no.2, 2018 , pp. 135-140 More about this Journal
Abstract
BACKGROUND: The Asian hornet (Vespa velutina nigrithorax), a wasp species, has attacked honey bee populations and affected the beekeeping industry in Korea over the past 15 years. However, little research has been done with this invasive species. In this study, we investigated the intestine bacterial microbiota of Asian hornets and honey bees to design an attractive trap for Asian hornets. METHODS AND RESULTS: Genomic DNAs isolated from the intestine microorganisms of Asian hornets and honey bees were utilized to amplify bacterial 16S rDNA for the comparative sequence analysis. The next generation sequencing analysis identified that the orders Flavobacteriales as the most abundant intestinal microorganisms in Asian hornets, showing a clear difference compared to honey bees in which Aeromonadales are dominant. We also report five newly identified 16S rDNA sequences of Asian hornet intestinal bacteria. According to the sequence blast search, these five bacteria belong to the genera Thalassomonas, Caedobacter, Vampirovibrio, Alkaliphilus and Calothrix. CONCLUSION: While Asian hornets and honey bees show similar intestine bacterial diversity, the relative ratio of bacterial populations is different. providing useful information to design pest control agents specifically targeting Asian hornets.
Keywords
Asian hornet; Honey bee; Intestine bacterial microbiota;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Barbet-Massin, M., Rome, Q., Villemant, C., & Courchamp, F. (2018). Can species distribution models really predict the expansion of invasive species?. PLoS One., 13(3), e0193085.   DOI
2 Budge, G. E., Hodgetts, J., Jones, E. P., Ostoja-Starzewski, J. C., Hall, J., Tomkies, V., Semmence, N., Brown, M., Wakefield, M., & Stainton, K. (2017). The invasion, provenance and diversity of Vespa velutina Lepeletier (Hymenoptera: Vespidae) in Great Britain. PLoS One, 12(9), e0185172.   DOI
3 Choi, Y. S., Lee, M. L., Lee, M. Y., Kim, H. K., Yoon, M. Y., & Kang, A. R. (2015). Trapping of vespa velutina nigrithorax Buysson (Hymenoptera: Vespidae) queen near apiaries honeybee comb extract in south region of korea. Journal of Apicultural Research, 30(4), 281-285.   DOI
4 Darrouzet, E., Gevar, J., Guignard, Q., & Aron, S. (2015). Production of early diploid males by european colonies of the invasive hornet Vespa velutina nigrithorax. PLoS One, 10(9), e0136680.   DOI
5 D'Argenio, V., & Salvatore, F. (2015). The role of the gut microbiome in the healthy adult status. Clinica Chimica Acta, 451(part A), 97-102.   DOI
6 Kang, E. J., Lee, M. L., Lee, M. Y., Kim, H. K., & Choi, Y. S. (2016). Field evaluation of Honeybee Pheromone traps for the Hornet Vespa velutina nigrithorax Buysson (Hymenoptera: Vespidae). Proceeding of the Apicultural Society of Korea, 116.
7 Kouduka, M., Suko, T., Morono, Y., Inagaki, F., Ito, K., & Suzuki, Y. (2012). A new DNA extraction method by controlled alkaline treatments from consolidated subsurface sediments. FEMS Microbiology Letters, 326(1), 47-54.   DOI
8 Monceau, K., Bonnard, O., & Thiery, D. (2014). Vespa velutina: a new invasive predator of honeybees in Europe. Journal of Pest Science, 87(1), 1-16.   DOI
9 Petrosino, J. F., Highlander, S., Luna, R. A., Gibbs, R. A., & Versalovic, J. (2009). Metagenomic pyrosequencing and microbial identification. Clinical chemistry, 55(5), 856-866.   DOI
10 Poidatz, J., Bressac, C., Bonnard, O., & Thiery, D. (2017). Delayed sexual maturity in males of Vespa velutina. Insect Science, 1-11.
11 Sauvard, D., Imbault, V., & Darrouzet, E. (2018). Flight capacities of yellow-legged hornet (Vespa velutina nigrithorax, Hymenoptera: Vespidae) workers from an invasive population in Europe. PloS one, 13(6), e0198597.   DOI
12 Sauvard, D., Imbault, V., & Darrouzet, E. (2018). Flight capacities of yellow-legged hornet (Vespa velutina nigrithorax, Hymenoptera: Vespidae) workers from an invasive population in Europe. PloS one, 13(6), e0198597.   DOI