• Proceedings of the Korea Society of Poultry Science Conference
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• 2005.11a
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• pp.78-79
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• 2005

Effects of dietary lactose or killed Salmonella on the performance, immune response and anti-oxidant system was studied in chicks innoculated with Salmonella typhimurium. In 27 days of age broiler, dietary lactose decreased performance, while dietary lactose and killed Salmonella elevated plasma peroxidase activity and IL-1 level in supernatant of PBMC stimulated with LPS. When broiler chicks innoculated with Salmonella, performance, activities of erythrocyte MnSOD and plasma peroxidase were enhanced after 7 days of the innoculation. Dietary lactose and killed Salmonella increased activity of erythrocyte MnSOD, plasma peroxidase, proliferation of PBMC stimulated with LPS and IL-1 level in the supernatant after 15 days of the innoculation. The result indicated that dietary lactose and killed Salmonella have modulated innate immune response and antioxidant system in broiler chicks innoculated with Salmonella typhimurium.

• Korean journal of food and cookery science
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• v.17 no.3
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• pp.211-217
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• 2001

The objective of this study was to evaluate the effect of functional herbal foods on the growth of intestinal lactic acid bacteria. When Bifidobacterium breve and human intestinal microflora were inoculated in the general anaerobic medium which contained each functional food water extract, most of functional herbal foods induced the growth of lactic acid bacteria by decreasing pH of the broth. The pH decreasing effects of Liriipe platyphylla and Platycodon grandiflorum were excellent. The growth of lactic acid bacteria effectively inhibited the bacterial enzymes, $\beta$-glucosidase and $\beta$ -glucuronidase. Eugenia caryophyllata and Liriipe platyphylla potently inhibited the productivity of P -glucosidase of B. breve and human intestinal bacteria. Cinnamomum cassia, Gardenia jasminoides and Platycodon grandiflorum potently inhibited the productivity of $\beta$-glucuronidase of human intestinal bacteria. The growth component isolated from Platycodon grandiflorum was sucrose (compound B).

• Journal of Life Science
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• v.9 no.1
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• pp.111-120
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• 1999

A bacteriocin-producing strain, J-105, was isolated from Kimchi and identified as Lactococcus sp. The optimum conditions for the bacteriocin production from the isolated microorganism were evaluated. For maximum yield of bacteriocin from Lactotoccus sp. J-105, the cell should be harvested at the early stationary phase and temperature, pH and NaCl concentration should be $25^{\circ}C$, pH 8.0 and without the addition of NaCl, respectively. Maltose should be used as a carbon source and organic nitrogen such as polypeptone should be used as a nitrogen source for the best yield. The bacteriocin from isolate was inhibitory against Acetobacter aceti, Bacillus subtilis and several strains of lactic acid bacteria. The bacteriocin of J-105 was sensitive to pepsin, but stable for heat treatment. It was stable even at autoclaving temperature for 15 min.

• KSBB Journal
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• v.9 no.5
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• pp.506-511
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• 1994

Antimicrobial activity of methanol extract and fraction from mugwort leaves(Artemisia princeps val. orientalis) was investigated for the screening of natural antiwucroblal components. By using agar diffusion method, ethyl acetate(EtOAc) layer fractionated from methanol extract of mugwort leaves showed the highest inhibitory effects against tested microorganisms. The ortho-coumaric acid(200∼600ppm) isolated from EtOAc layer showed strong antibacterial activities for Bacillus subtilis and Salmonella typhimurium. As derivatives of o-coumaric acid, antibacterial activity of para-coumaric acid was 1.2∼1.7 fold higher than that of o-coumaric acid. Three types of coumaric acids strong inhibited the growth of B. subtilis in the culture medium. Growth of S. tyhimurium, P. aeruginosa and S. aureus were effectively inhibited by o-, m- and p-coumaric acids, respectively. Minimum inhibitory dose of p-coumaric acid for B. subtilis was $\100∼200mu\textrm{g}$/paper disk.

• Korean Journal of Food Science and Technology
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• v.32 no.6
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• pp.1379-1388
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• 2000

Ethanol extracts from Ruta graveolens Linne exhibited strong antimicrobial activities by disc diffusion method against 5 strains of Listeria monocytogenes (ATCC 19111, ATCC 19112, ATCC 19113, ATCC 19114 and ATCC 15313). Ethanol extract from Ruta graveolens Linne was subsequently fractionated by n-hexane, chloroform, ethyl acetate and water. Chloroform fraction of Ruta graveolens Linne showed strong growth inhibition at concentrations as low as 40 ppm level in broth culture medium against 5 strains of L. monocytogenes for 72 hr at $30^{\circ}C$. Single substance(RTG1-1) was isolated by silica gel column chromatography from chloroform fraction of Ruta graveolens Linne. RTG1-1 showed a strong bactericidal activity against L. monocytogenes at a concentration of 20 ppm level. Purified RTG1-1 was identified as gravacridonechlorine by analyses of EI-Mass, $^1H-NMR$ and $^{13}C-NMR$.

• Korean Journal of Food Science and Technology
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• v.28 no.3
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• pp.604-608
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• 1996

The antimicrobial activty of leek (Allium tuberosum) was investigated against 17 strains of microorganisms. Methanol extracts of leek showed the growth inhibition effects on the wide range of microorganisms including gram positive bacteria, gram negative bacteria and yeasts. The extracts were analysed by using solvent fractionation, silica gel adsorption chromatography, Sephadex LH-20 column chromatography, TLC, silica gel partition chromatography and HPLC techniques. Six components whose molecular weights range from 200 to 400 were confirmed to have the antimicrobial activity.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2002.05b
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• pp.207-208
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• 2002

일반적으로 미생물은 생육조건이나 환경인자의 변화에 따라 활성과 증식속도간의 차이가 나타난다. 따라서 환경에 적용시 환경인자의 영향을 고려하여 보다 높은 분해효육 조건을 선정할 필요가 있다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.04a
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• pp.144-145
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• 2003

• Proceedings of the Korean Society of Applied Pharmacology
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• 1997.04a
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• pp.84-84
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• 1997

장내세균은 음식물, 스트레스, 생활환경에 의해 영향을 받으며 그 결과 장내 세균이 생산하는 효소활성도 영향을 받는다. 장내미생물효소는 질병과 밀접한 관계를 갖고 있으며 장내의 pH와 효소저해제에 의해 영향을 받는 데 장내의 높은 pH에 의해 $\beta$-glucosidase, $\beta$-glucuronidase, tryptophanase 등의 장내유해효소활성이 유도되므로 장내의 pH를 낮춤으로써 효소활성을 저하시킬 수 있다. Bifidobacterium은 장내에서 lactic acid, acetic acid를 생산하여 장내의 pH를 낮추며 유해균의 증식을 억제하고 유해효소의 활성을 억제하는 역할을 할 것으로 기대된다. 특히 한국인으로부터 분리된 유산균일 경우 한국인의 장내에 가장 잘 정착되며 장내미생물의 유해효소를 효과적으로 억제할 것으로 생각된다.

• Microbiology and Biotechnology Letters
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• v.16 no.2
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• pp.126-130
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• 1988

Promoters of an alkali-tolerant Bacillus sp. isolated from soil have been cloned in Bacillus subtilis using promoter probe vector pPL703. The CAT specific activity of a clone harboring the strongest promoter activity among these transformants was 8.01. This activity was 2.5 times higher than that of Bacillus subtilis harboring expression vector pPL708 and was increased after the end of the logarithmic growth phase. In the 2.8kb of inserted DNA fragment, BamHI and Sal I recognition sites were located.