• Journal of Korean Society of Forest Science
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• v.100 no.3
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• pp.449-454
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• 2011

This experiment was conducted to evaluate growth rate among 15 embryogenic tissue lines (ETLs), comparison of maturation on somatic embryos (SEs) with 13 ETLs and efficiency with various concentrations of gelrite on SEs germination in Japanese red pine (Pinus densiflora). In comparison of ETLs growth rate (folds) with 15 lines, the 05-4 line (5.3 folds) showed the highest rate, on the other hand, the lowest one was recorded in the line of 05-37 (1.4 folds). The 13 ETLs were tested for the extent of SEs production. The best production was recorded in the line of 05-4 (39.8/90 mg F.W.). However, most of ETLs, except 5 lines (05-4, 12, 21, 29 and 37), did not produce SEs at all, therefore, big differences in the ability of SEs production existed among the ETLs tested. Effects of various gelrite concentrations for SEs germination with 3 ETLs were also compared. The highest result was obtained from 0.2% gelrite concentration with 05-4 line (47.3%), there was a inclination that the rate of germination was gradually declined over 0.2% gelrite concentration with the 05-4 and 29 lines. respectively. In contrast, in the line of 05-37, no SEs germination occurred on medium with 0.1 or 0.2% gelrite. In conclusion, the growth rate, SEs production and germination frequency were appeared to deeply depended on the ETLs.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.10a
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• pp.19-19
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• 2010

황칠나무(Dendropanax morbifera Lev.)는 두릅나무과(Araliaceae)에 속하며 학명에서 뜻하는 바와 같이 목본 (Dendro), 전능약(Panax)이라는 의미가 있고 나무인삼이라 불리기도 하며 줄기에 상처를 내면 노란액이 나온다고 해서 황칠나무(D. morbifera)라는 이름이 붙여졌다. 두릅나무과는 우리나라에서 최고의 약재들로 손꼽히는 인삼(Panax ginseng), 가시오갈피(Eleutherococcus senticosus) 등의 약용식물을 포함하고 있어서 황칠나무는 황칠수지액 이외에 약용식물로서의 무한한 개발 가능성을 내포하고 있다. 따라서 본 실험은 황칠나무의 기내 부정근 유도 및 증식조건의 확립을 목적으로 수행되었다. 우선 황칠나무의 기내 발아체로부터 부위(잎, 줄기, 뿌리)를 달리하여 부정근을 유도한 결과, 잎은 줄기나 뿌리보다 양호한 부정근의 유도를 보였다. 또한 유도된 부정근을 이용하여 옥신의 종류에 따른 부정근 유도율을 조사한 결과 IBA와 NAA는 IAA와 2.4-D보다 높은 유도율을 보였다. IBA의 농도에 따른 유도율과 증식효율은 IBA가 1.0 mg/L 첨가되었을 때 가장 높은 유도 및 증식효율을 보였다. 최적의 액체배지조건을 확인하고자 sucrose의 농도와 염농도를 달리하여 실험한 결과 1/2MS 배지는 MS 배지보다 10%정도 높은 증식율을 보였다. 액체배양 된 황칠나무의 부정근을 각각 1/2MS 배지에 30 g/L sucrose, 3.0 mg/L IBA가 첨가된 5 L volume 생물반응기에 4주 간 배양한 대조구와 2주 후 IBA의 농도를 1.0으로 낮추어 배양한 실험구에서 2주후 IBA의 농도를 낮추어 배양한 실험구에서 대조구보다 약 2배 높은 부정근의 증식량을 보였다. 결국, 황칠나무의 종자발아체를 이용하여 부정근의 유도 및 증식조건에 필요한 기내배양조건을 확립하였고, 플라스크와 생물반응기 배양을 통해 효율적인 실험실 내 증식조건을 확립하였다. 본 실험결과는 향후 황칠나무 천연추출물을 활용한 향장품/식,의약품 소재의 대량확보 차원에서 중요한 가치를 내포하고 있다고 조심스럽게 사료된다.

• The Korean Journal of Malacology
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• v.23 no.1
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• pp.51-57
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• 2007

This study examined the possibility on the dietary value and cryopreservation of the marine microalga, Isochrysis galbana. Four cryoprotectants, dimethyl sulfoxide (DMSO), ethylene glycol (EG), glycerol (Gly) and 1.2-propanedial (PD) were tested at the concentrations of 1.0, 1.5, 2.0, 2.5 and 3.0 M respectively. The highest survival rates were obtained with 1.5 and 2.0 M of four cryoprotectants yielding a survival rate of 80%. Cell concentration of $30\;{\times}\;10^4\;cell/ml$ at the initial point of the experiment was increased to $365\;{\times}\;10^4\;cell/ml$ in 1.5 M of dimethyl sulfoxide, $298\;{\times}\;10^4\;cell/ml$ in 1.5 M of ethylene glycol, $512\;{\times}\;10^4\;cell/ml$ in 1.5 M of glycerol, and $385\;{\times}\;10^4\;cell/ml$ in 1.5 M of 1.2-propanedial after five days, respectively. In dietary value experiment, survival rate and growth were not significantly different.

• Korean journal of applied entomology
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• v.62 no.3
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• pp.139-147
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• 2023

Leaf-spray in vitro bioassays appraise new aphicidal formulations for managing deleterious plant-feeding aphids. The formulation may utilize alternative and integrated strategies. However, leaf spraying even under controlled conditions may affect aphid reproduction and mortality. This study examines leaf spray applications for optimum and reproducible aphicidal results using tobacco leaves overlaid on cotton fabric or water agar surfaces. Infestation of the undersides of tobacco leaves with nymphs of green peach aphids was used in the assays. Spray distance and volume were optimized using water-sensitive paper to ascertain the best surface coverage. Overlays of the leaves on water agar caused less mortality and greater reproduction than the use of cotton fabric. The relative humidity of the insect-rearing chambers changed with the watering regime for the insect - rearing chambers with cotton fabric; 60% relative humidity was optimal. Relative humidity was not affected by the concentration of agar in the water agar chambers. Applications of the chemical aphicidal standard, Sulfoxaflor, under the optimized conditions exhibited similar times for lethality although the rate was faster with leaves on the cotton fabric than on water agar. These studies establish reproducible and sensitive techniques for assessing the lethality and effects on reproduction of potential aphicidal products.

• Journal of Life Science
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• v.12 no.6
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• pp.740-744
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• 2002

This experiment was conducted to clarify the effects of physiological properties in culture media on the proliferation and the acclimation of bulblets of several kinds of lilies cultured in vitro. Proliferation rate of bulblets from bulblet of Oriental hybrid ‘Marco Polo’, ‘Casa Blanca’ and Asiatic hybrid ‘Jolanda’ was higher in solid MS media compared with bulblets from scale Acclimation rate of regenerated bulblets was high in solid media which was pre-cultured in vitro, compared with liquid media. Sprouting rate of bulblets from bulblet was higher than bulblets from scale.

• Korean Journal of Plant Resources
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• v.15 no.3
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• pp.221-226
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• 2002

This experiment was conducted to establish the mass propagation system of Calanthe discolor Lindley. When the Calanthe discolor seeds were sown in Murashige and Skoog medium, the percentage of germination was 65％. Seedlings grew more rapidly in the liquid medium than the solid medium. All regenerated plantlets were survived in acclimatized condition of 70% shade and more than 80％ humidity. Also, we found out that the 88% of survival ratio could be achieved in containing soil mixture of vermiculite and perlite as same as amount.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2004.05a
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• pp.314-317
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• 2004

IGF-I rich fraction 분리는 분만 후 24시간이내에 착유한 젖소 초유를 30kDa과 1kDa Ultrafiltra-tion(UF) membrane을 이용하여 분리하였다. 분리한 IGF-I rich fraction은 sandwich ELISA로 정량한 결과 1mg/ml 각 암세포에 미치는 영향을 실험하였다. 그 결과IGF-I rich fraction $1mg/m{\ell}$의 농도로 처리하였을 때 A-427ce11은 33%, SK-HEP-1 cell은 약 2%, A498 cell은 약 22%의 암세포 성장저해 효과를 보였고 HeLa는 약 5% 이하의 세포 증식 저해율과 WiDr cell은 약 30%의 세포성장 저해율을 나타내었으며, 유일하게 위암 세포주인 SNU 16에 대해서는 $1mg/m{\ell}$과 $100{\mu}g/m{\ell}$ 및 $10{\sim}1{\mu}g/m{\ell}$의 농도에서 약 15%와 10% 및 약 5% 이하의 세포 증식율을 나타내었다.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.4
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• pp.550-555
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• 1997

Vibrio cholerae non-O1 (V. cholerae non-O1) was previously called nonagglutinable or noncholera vibrios, since it fails to react with polyvalent O1 antisera. This organism is biochemically and genetically indistinguishable from V. cholerae O1 except serological difference. V. cholerae non-O1 strains are often detected in the environment including bays, estuaries, and fresh water, and also found in food. Therefore it is designated food borne bacterium in Japan. However, research papers on V. cholerae non-O1 are very rare in Korea. In order to investigate bacteriological characteristics of V. cholerae non-O1, we isolated V. cholerae non-O1 from the environmental sea water. Among the isolated V. cholerae non-O1 strains, we selected the strain which had the most strong hemolytic activity, named as V. cholerae non-O1 FM-3. The optimum growth conditions of V. cholerae non-O1 FM-3 were $37^{\circ}C$ and pH 8.5 in BHI broth (containing $0.5\%$ sodium chloride), and it grew better than V. cholerae non-O1 ATCC 25872. But both were not able to grow in BHI broth added $5.0\%$ of sodium chloride or adjusted to pH 5.0. According to the experimental results on the susceptibility test against various antibiotics, there were no significant differences between the isolated strain and reference strain (V. cholerae non-O1 ATCC 25872). Most of the antibiotics examined had bacteriostatic action against V. cholerae non-O1 FM-3 while vancomycin, oxacillin, colistin, polymyxin B, and sulfadiazine had no bacteriostatic activity.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.25-25
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• 2020

산초나무(Zanthoxylum schinifolium Siebold & Zucc.)는 운향과(Rutaceae)에 속하는 낙엽활엽관목으로 주로 열매, 잎, 줄기는 식·약용으로 이용한다. 최근 산초나무 부위별 추출물에서 항산화활성, 항염증효과, 미백효과 등에 관한 연구가 이루어지면서 산초나무의 가치가 더욱 증가하고 있다. 본 연구는 산초나무의 효율적인 유·무성 증식법을 구명하기 위해 종자 처리에 따른 발아특성, 생장조절물질 처리에 따른 가지삽목과 뿌리삽목의 발근특성을 조사하였다. 산초나무 종자는 3개월간 저온습사저장(종자:모래=1:1, -4℃)을 실시할 경우 약 64.0%가 발아하였다. 습사저장된 종자를 파종 직전에 GA3를 이용하여 0, 500, 1,000 및 2,000ppm 농도에서 처리한 결과 각 처리구별 발아율은 유의한 차이를 나타내지 않았다(F=1.556, p=0.260). 다만, 500ppm 농도에서 72.0%가 발아하여 가장 높은 발아율을 보였으며 1,000ppm 이상 처리구에서는 발아율이 다소 감소하는 경향을 나타냈다. 무성증식 방법은 2월 중순에 산초나무 가지삽수와 뿌리삽수를 채취한 후 IBA와 NAA를 각각 0, 500, 1,000 및 2,000ppm 농도로 처리하였다. 가지삽목을 실시한 대부분의 삽수에서 신초가 발생했으나, 발근율은 1.3~6.3%로 현저히 낮았다. 반면, 뿌리삽목은 3개월 후 부정아 발생율이 처리에 따라 58.3~83.3%로 조사되었으며, 발근율은 0~66.7%를 나타나 처리구별 발근율에 유의한 차이가 있었다(F=3.744, p=0.020). IBA 처리가 근삽수의 발근에는 효과적이었으며, 특히 IBA 2,000ppm 처리에서 66.7%의 발근율을 보여 가장 효과적임을 알 수 있었다. 결론적으로, 산초나무의 효율적인 유성증식을 위해서는 종자를 3개월간 저온습사저장하는 것이 효과적이며, 무성증식 방법으로는 가지삽목보다 뿌리삽목을 이용하는 것이 더 효과적인 것으로 판단된다.

• Food Science and Preservation
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• v.24 no.2
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• pp.289-293
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• 2017

Ethanol extract of garlic peels (GPE) was investigated for its antiproliferative effects on human cancer cell lines. Human lung cancer cell line A549 treated with $500{\mu}g/mL$ GPE resulted in the growth inhibition of A549 by 90%. In stomach cancer cell AGS proliferation inhibition activity, GPE showed 45% and 71% inhibition of AGS growth at $1,000{\mu}g/mL$ and $2,000{\mu}g/mL$, respectively. GPE inhibited the growth of the breast cancer cells MCF-7 effectively at low concentration and showed 78% and 90% inhibitions of MCF-7 growth at $200{\mu}g/mL$ and $500{\mu}g/mL$, respectively. GPE showed very significant antiproliferation effect on liver cancer cell line Hep3B and inhibited Hep3B cell growth by 57% at $100{\mu}g/mL$, and the inhibition's rate increased up to 87% at $500{\mu}g/mL$. Antiproliferation effect of GPE on colorectal cancer cell HT-29 showed 15% reduction of HT-29 cell growth at $200{\mu}g/mL$ and the growth rate was reduced in a dose dependent manner up to $1,000{\mu}g/mL$. These results indicated that GPE had high antiproliferation effects on breast and liver cancer cell lines at low concentrations ($200{\mu}g/mL$), and by higher concentrations over $500{\mu}g/mL$, GPE inhibited the growth of A549 and HT-29. The results of our study suggested the potential use of garlic peels for use as an excellent antiproliferative substance for human cancer cells.