• Journal of the korean academy of Pediatric Dentistry
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• v.33 no.2
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• pp.221-232
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• 2006

The purpose of this study was to investigate the effect of step-curing mode on polymerization shrinkage and contraction of composite resin restoration. Class I cavities were prepared on the extracted human premolars. The cavities were ailed with Filtek $Z-250^{TM}$ (hybrid resin, 3M ESPE, USA) and Filtek $flow^{TM}$ (flowable resin, 3M ESPE, USA) and cured with one of the following irradiation modes; Halogen 40sec with continuous curing, LED 10sec with continuous curing, and LED 13sec with step-curing. Contraction stress was measured with strain gauge which was connected to TML $Datalogger^{TM}$ (TDS-102, SOKKI, Japan) and resin-dentin interfaces were observed by scanning electron microscope. The results of present study can be summarized as follows : 1. Composite resin restoration showed transient expansion just after irradiation of curing light. Contraction stress was increased rapidly at the early phase of polymerization and reduced slowly as time elapsed (P<0.05) 2. $Filtek\;flow^{TM}$ showed lower contraction stress than Filtek $Z-250^{TM}$ regardless of curing modes. 3. LED step-curing mode showed lowest contraction stress in Filtek $Z-250^{TM}$ compared with other curing modes(P<0.05). 4. LED step-curing mode showed lowest contraction stress in $Filtek\;flow^{TM}$ compared with other curing modes(P<0.05), but difference in contraction stress was not so greate as in $Filtek\;Z-250^{TM}$. 5. Polymerization of composite resin by LED light with step-curing mode and halogen light with continuous ode resulted in better marginal sealing than LED light with continuous mode.

• Restorative Dentistry and Endodontics
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• v.34 no.5
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• pp.397-405
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• 2009

This study compared the effect of an activator, intermediate bonding resin and low-viscosity flowable resin on the microtensile bond strength of a self-curing composite resin used with two-step total etching adhesives. Twenty extracted permanent molars were used. The teeth were assigned randomly to nine groups (n=10) according to the adhesive system and application of additional methods (activator, intermediate adhesive, flowable resin). The bonding agents and additional applications of each group were applied to the dentin surfaces. Self-curing composite resin buildups were made for each tooth to form a core, 5mm in height. The restored teeth were then stored in distilled water at room temperature for 24h before sectioning. The microtensile bond strength of all specimens was examined. The data was analyzed statistically by one-way ANOVA and a Scheffe's test. The application of an intermediate bonding resin (Optibond FL adhesive) and low-viscosity flowable resin (Tetric N-flow) produced higher bond strength than that with the activator in all groups. Regardless of the method selected, Optibond solo plus produced the lowest ${\mu}TBS$ to dentin. The failure modes of the tested dentin bonding agents were mostly adhesive failure but there were some cases showed cohesive failure in the resin.

• Korean Chemical Engineering Research
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• v.48 no.5
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• pp.609-614
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• 2010

In this study, emulsion polymerizations for synthesizing acrylic pressure-sensitive adhesive(PSA) were carried out using 2-ethylhexyl acrylate(2-EHA), n-butyl acrylate(n-BA), methyl metacrylate(MMA) as fundamental monomers and acrylic acid(AAc) as a functional monomer in the presence of anionic SLS (sodium lauryl sulfate). To obtain the optimized synthetic condition in the polymerization, we analyzed the polymerization variables such as the effect of surfactant concentration and hydrophilic lipophilic values(HLB). At the same time, the final adhesive properties were also analyzed by the function of the initiator concentration and buffer concentration. In the results, the most stable emulsion was obtained at the surfactant concentrations between 3 and 5 wt%. It was also determined the effect of HLB value of nonionic surfactant and the initiator concentrations on the gel content. Stable emulsion is obtained using the surfactant having HLB value of 12.3. The rate of emulsion polymerization was increased at the initiator concentration greater than 1 wt%, but the stability of the emulsion was decreased. Finally, the effect of the buffer concentrations on the pH and the conversion of the acrylic emulsion product were experimentally measured. At the sodium bicarbonate concentration above 0.4 wt%, the buffer infulence was apparent. The buffer effect was fully acceptable at the concentrations between 0.6 and 0.8 wt% regardless of the monomer composition.

• Korean Journal of Clinical Laboratory Science
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• v.48 no.2
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• pp.88-93
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• 2016

Dirofilaria immitis is a filarial nematode parasite that causes cardiopulmonary dirofilariasis in dogs. The purpose of this study was the development of real-time PCR assays for efficient detection of D. immitis. The D. immitis-specific primers confirmed in our previous study and a newly designed TaqMan probe were used for quantitative diagnostics. First, SYBR Green real-time PCR was performed using the specific primers and serially diluted genomic DNA or plasmid DNA, and melting curve analyses were performed after amplification. The melting curve showed one specific peak in each of the genomic and plasmid DNA reactions, suggesting that the primers specifically amplify the D. immitis cytochrome c oxidase subunit I gene. Comparison of SYBR Green and TaqMan real-time PCR using serially diluted plasmid DNA showed higher efficiency and specificity with TaqMan real-time PCR. The real-time PCR assays developed in this study will provide improved diagnostic methods to overcome the limitations of conventional diagnostic tools and facilitate more rapid and accurate diagnoses.

• Applied Chemistry for Engineering
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• v.21 no.1
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• pp.34-39
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• 2010

Methacryloxypropyltrimethoxysilane (MPTMS) was used for the surface modification of silica nanoparticles in the toluene dispersion system for 8 h (MPSN). Then, methacryloxypropyl-modified silica nanoparticles were successfully prepared by solutioun polymerization in the ethanol solution at $60^{\circ}C$ for 14 h with adding AIBN initiator. The modification of ultra-fine particles (SiOx-PAA nanospheres) was investigated via EA, XPS, FTIR, TGA, SEM and TEM. The mean diameter of the bare silica nanoparticles, MPSN and SiOx-PAA monodisperse nanoparticles was about 25, 30 and 35 nm, respectively.

• Journal of Life Science
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• v.9 no.2
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• pp.146-152
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• 1999

In order to elucidate a role of residue 24 in the folding of tryptophan synthase $\alpha$ subunit, mutant proteins in which Thr 24 was replaced by Met, Ala, Ser, Leu or Lys were overexpressed in E. coli, and the extents of accumulated proteins as soluble or aggregated forms were examined. The mutant proteins with Met or Leu at residue 24 were predominantly accumulated as soluble forms as the native protein. On the other hand, mutant proteins with Ser, Ala or Lys at residue 24 were expressed as aggregated forms as well. This result suggests that residue 24 of tryptophan synthase $\alpha$ subunit may be implicated in the folding of this protein.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2004.04a
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• pp.83-87
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• 2004

본 연구는 고해상도의 충적층 지하수위 분포 조사를 위한 탄성파 굴절법 조사 방법을 소개하고 부여 군수리 충적층 일대에서 이 기법을 통해, 획득된 실제 충적층내의 지하수위 조사 결과를 제시한다. 기본적으로 본 연구에서 활용된 연속 굴절파 중합 방식은 동일 공심점(common mid point, 이후 CMP)을 갖는 굴절파 신호를 취합하고, 이격 거리(offset)에 대한 시간 지연 효과 보정을 수행한 후, 이들 신호를 중합하여, 충적층의 지하수위면에서 굴절된 신호를 보다 뚜렷이 부각시켜 정확한 지하수위 정보를 획득 하는 방식으로 일명 CMP 굴절법이라고도 한다. 이 방식은 독일에서 최초 개발되었으나(Gebrande, 1986; Orlowsky 등, 1998), 국내에서 적용되기는 본 연구가 최초이다. 이러한 탄성파의 굴절 신호를 사용하는 방식은 우선, 기존의 일반적인 고해상도 반사법 탐사에서 잡음으로 여겨졌던 굴절파 신호를 활용할 수 있으며, 고해상도 반사법 탐사와 동일한 배열과 운영 방식으로 획득된 자료에서 원하는 정보를 획득할 수 있으므로, 고해상도 반사법에 의한 기반암 조사와 함께 적용될 경우, 정화한 충적 대수층의 분포를 조사할 수 있게 하여주는 획기적인 조사 신기술이다. 개발된 기법은 부여 군수리 충적층 지역을 대상으로 적용되었으며, 그 결과 기존의 어떠한 지구물리 조사 방법보다 정확하고 분명한 지하수위 분포를 보여주었다.

• Journal of Navigation and Port Research
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• v.28 no.3
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• pp.199-205
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• 2004

We have developed a composite grid method for the solution of the radiation problem We divide the domain into two different grids; one is a moving grid system and the other is a fixed grid system. This numerical method is applied to calculation of the radiation forces generated by the submerged plate oscillating near a free surface. The experimental data are compared with the numerical ones obtained by the present method and a linear potential theory. As a result, we can confirm the accuracy of the present method. Finally, Lie have evaluated the effect of nonlinear and viscous damping on the hydrodynamic forces acting on the submerged plate.

• Korean Chemical Engineering Research
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• v.52 no.3
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• pp.378-381
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• 2014

We studied the pasteurization effect of the microorganism involved in the water, using ion-exchanging filter made of the Radiation Induced Grafted Polymerization. This ion-exchanging filter is made by the graft polymerization of GMA, after irradiation of electron beam to the non-woven filter. Then, we made the ion-exchanging filter (EtA, DEA, SS) applying ion-exchanging base to the GMA filter. As a result, the density of the ion-exchanging base is shown as 2.38 mol/kg in case of EtA, 1.79 mol/kg in case of DEA and 0.75 mol/kg in case of SS. Through this filter made by this method, we measured the pasteurization power of E. coli. We found very high elimination rate such as log 4.65 in case of SS-dial filter, which is higher as 3.00 times in comparison with EtA, and 1.10 times in comparison with DEA. This data show the result is very excellent comparing with 3,000 CFU/ml. of city water treatment basis.

• Polymer(Korea)
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• v.36 no.5
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• pp.599-605
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• 2012

The physicochemical properties such as surface hardness, solvent mechanical wear resistance, and resistance to scratch properties of poly(3,4-ethylenedioxythiophene) (PEDOT) thin film prepared by vapor phase polymerization (VPP) was effectively improved by post-treatment of various metal alkoxide sol solutions. Metal oxide layer derived from sol-gel process of metal alkoxide was generated on the PEDOT thin film layer by VPP, resulting in improving mechanical properties of the conductive thin films without any deterioration of their original surface resistance. Several kinds of silicone and titanium alkoxide derivatives with various functional groups were used as metal alkoxide sol sources. Among them, PEDOT-metal oxide composite thin film derived tetraethyl orthosilicate showed the best performance in the terms of surface resistance, transmittance, and various physicochemical properties. The effect of metal alkoxide content in washing solution, oxidant content and drying temperature have been investigated in order to optimize the various properties of PEDOT-metal oxide composite thin film.