• The Journal of Korean Physical Therapy
• /
• 제13권3호
• /
• pp.569-578
• /
• 2001

The purpose of the present study was to examine the functional recovery of the crushed sciatic nerve of rats after low-power laser irradiation applied to the corresponding segments of the spinal cord. After a crushed injury on the left sciatic nerve in rats. low-power laser irradiation was applied transcutaneously to corresponding segments of the spinal cord immediately after suture the wound by using 2000 mW, 2000Hz, 830 nm CaAIAs(Gallium-aluminum-arsenide) semiconductor diode laser. The laser treatment was performed with 10 minutes daily for 4 successive weeks. Functional recovery was evaluated per weekly following injury by sciatic function index(SFI),using data obtained by walking track analysis. For four weeks after crush injury, experimental group had significantly greater functional improvement than control group(${\alpha}$=0.05). In a experimental group, SFI was significantly increased for three weeks, but control group not increased for two weeks. This study suggests that low-power laser irradiation applied directly to the spinal cord can improve functional recovery of the crushed sciatic nerve in rats.

• The Journal of Korean Physical Therapy
• /
• 제18권1호
• /
• pp.65-73
• /
• 2006

Purpose: The purpose of this study was to identify the effect of electroacupuncture(EA) and transcutaneous electric nerve stimulation(TENS) after sciatic nerve crush injury in rats. Methods: The EA for experimental group I (Exp I, n=15) and TENS for experimental group II (Exp II, n=15) was applied from post-injury day(PD) 1 to PD 14 after sciatic nerve injury using low frequency stimulator that gave electrical stimulation(15min/60Hz). In order observe the effect of EA and TENS, this study examined GAP-43 expression in rat lumbar spinal cord at the PD 1, PD 7 and PD 14. In addition, the stride length(SL) and toe out angle(TOA) were measured at the PD 7 and PD 4. Results; Exp I and Exp II had higher GAP-43 immunoreactivity than control group(PD 1, 7, 14). The SL of Exp I and Exp II were significantly higher than control group(PD 7, 14). The TOA of Exp I and Exp II were significantly lower than control group(PD 7, 14). Conclusion: EA and TENS application increased motor nerve recovery and expression of GAP-43 immunoreactivity after sciatic nerve crush injury. Therefore effect of TENS and EA had similar effect on nerve regeneration and functional recovery.

• 대한한의학회지
• /
• 제31권1호
• /
• pp.93-111
• /
• 2010

Objectives: The present study was performed to evaluate the potential effects of Bupleuri radix (SH) on regenerative activities in the peripheral sciatic nerve after crushing injury in rats. Methods: Axonal regeneration after crush injury in rats was analyzed by immunofluorescence staining using anti-NF-200 antibody and retrograde tracing of DiI-axons. Changes in protein levels in the sciatic nerve axons and DRG tissue were analyzed by Western blot analysis and immunofluorescence staining. Effects of SH extract treatment on neurite outgrowth was examined by immunofluorescence staining for cultured DRG neurons. Results: Major findings on the effects of SH extract treatment on axonal regeneration are summarized as follows. 1. SH-mediated enhancement in axonal regeneration was identified by immuno- fluorescence straining of NF-200 protein and retrograde tracing of DiI-labeled axons. 2. Axonal GAP-43 protein levels were upregulated by SH not only in the injured axons but also in the DRG sensory neurons corresponding to sciatic sensory axons. 3. Phospho-Erk1/2 protein levels were increased in both injured axonal area and DRG sensory neurons by SH. Phospho-Erk1/2 was also found in non-neuronal cells in the injured axons. 4. SH elevated levels of Cdc2 protein produced in Schwann cells in the distal portions of injured sciatic nerves. 5. The neurite outgrowth of DRG sensory neurons in culture was augmented by SH, and these changes were positively associated with GAP-43 production levels in the DRG neurons. Conclusions: These data suggest that SH extract improves the regenerative responses of injured peripheral neurons, and thus may be useful for understanding molecular basis for the development of therapeutic strategies.

• 한방재활의학과학회지
• /
• 제21권2호
• /
• pp.125-142
• /
• 2011

Objectives : Peripheral nerve injuries are commonly encountered clinical problems and often result in severe functional deficits. The purpose of this study was to evaluate the effects of Haein-tang(Hairen-tang) extract on functional recovery and pain release in the sciatic nerve after crushed sciatic nerve injury in rats. Methods : 1. Sciatic functional index(SFI) were performed on functional recovery. 2. c-Fos immunohistochemistry were performed on c-Fos expressions in the paraventricular nucleus(PVN) and ventrolateral periaqueductal gray(vIPAG). 3. Neurofilament immunohistochemistry were performed on neurofilament regeneration. 4. Western blot were performed on brain-derived neurotrophic factor(BDNF) and nerve growth factor(NGF) expression. Results : 1. Haein-tang(Hairen-tang) extract significantly enhanced the SFI value in the sciatic nerve injury and 100 mg/kg, 200 mg/kg Haein-tang(Hairen-tang)-treated group. 2. Haein-tang(Hairen-tang) extract significantly suppressed the sciatic nerve injury-induced increment of c-Fos expressions in the PVN and vIPAG in the sciatic nerve injury and 100 mg/kg, 200 mg/kg Haein-tang(Hairen-tang)-treated group. 3. Haein-tang(Hairen-tang) extract significantly increased neurofilament expression in the sciatic nerve injury and 50 mg/kg, 100 mg/kg, 200 mg/kg Haein-tang(Hairen-tang)-treated group. 4. Haein-tang(Hairen-tang) extract significantly controled the sciatic nerve injury-induced increment of BDNF and NGF expressions in the sciatic nerve injury and 100 mg/kg, 200 mg/kg Haein-tang(Hairen-tang)-treated group. Conclusions : These results suggest that Haein-tang(Hairen-tang) treatment after sciatic nerve injury is effective for the functional recovery by enhancing of axonal regeneration and suppressing of pain.

• 한방재활의학과학회지
• /
• 제23권2호
• /
• pp.33-48
• /
• 2013

Objectives : Peripheral nerve injuries are commonly encountered clinical problems and often result in severe functional deficits. Sukjiyanggeun-Tang(shudiyangjin-tang), in oriental medicine, has been used to treat various musculoskeletal disorders. Methods : In the present study, the effects of aqueous extract of Sukjiyanggeun-Tang(shudiyangjin-tang) on functional recovery, severity of pain, and expressions of neurofilament, cycloxygenease-2(COX-2), inducible nitric oxide synthase(iNOS), and tumor necrosis factor-${\alpha}$(TNF-${\alpha}$) following sciatic crushed nerve injury in rats were investigated. For this study, walking tract analysis, plantar test, western blot analysis for COX-2 iNOS, and TNF-${\alpha}$, and Immunofluorescence test for neurofilament were performed. Results : In the present results, sciatic functional index(SFI) in walking tract analysis was significantly decreased following sciatic crushed nerve injury, and pain severity in plantar test was significantly increased. COX-2, iNOS and TNF-${\alpha}$ expressions were increased whereas neurofilament expression was decreased by sciatic crushed nerve injury, In contrast, treatment with Sukjiyanggeun-Tang(shudiyangjin-tang) improved SFI in walking tract analysis and suppressed the pain severity in sciatic crushed nerve injury. Sukjiyanggeun-Tang(shudiyangjin-tang) treatment also suppressed COX-2, iNOS, and TNF-${\alpha}$ expressions and enhanced the neurofilament expression in sciatic crushed nerve injury. Conclusions : In the present study, we have shown that Sukjiyanggeun-Tang(shudiyangjin-tang) is the effective therapeutic modality to ameliorate the symptoms of sciatic crushed nerve injury.

• 한방재활의학과학회지
• /
• 제23권2호
• /
• pp.17-31
• /
• 2013

Objectives : Peripheral nerve injuries are commonly encountered clinical problems and often result in serve functional deficit. Bee venom and scolopendra subspinipes have been traditionally used in oriental medicine to treat several inflammatory diseases and chronic pain conditions. Methods : In the present study, the effects of bee venom pharmacopuncture and scolopendra subspinipes pharmacopuncture on functional recovery, severity of pain, and expressions of neurofilament, cycloxygenease-2(COX-2), and tumor necrosis factor-${\alpha}$(TNF-${\alpha}$) following sciatic crushed nerve injury in rats were investigated. For this study, walking tract analysis, plantar test, western blot for COX-2 and TNF-${\alpha}$, and immunohistochemistry for neurofilament were performed. Results : In the present results, sciatic functional index(SFI) in walking tract analysis was significantly decreased following sciatic crushed nerve injury, and pain severity in plantar test was significantly increased. COX-2 and TNF-${\alpha}$ expressions were increased whereas neurofilament expression was decreased by sciatic crushed nerve injury. On the other hand, bee venom pharmacopuncture and scolopendra subspinipes pharmacopuncture improved SFI in walking tract analysis and suppressed the pain severity in sciatic crushed nerve injury. Bee venom pharmacopuncture and scolopendra subspinipes pharmacopuncture suppressed COX-2 and TNF-${\alpha}$ expression and enhanced the neurofilament expression in sciatic crushed nerve injury. Conclusions : In the present study, we have shown that treatment with bee venom or scolopendra subspinipes is the effective therapeutic modality to ameliorate the symptoms of sciatic crushed nerve injury. The efficacies of bee venom and scolopendra subspinipes were similar.

• 혜화의학회지
• /
• 제29권1호
• /
• pp.18-25
• /
• 2020

Objective: The goal of this study is to investigate whether peripheral axonal regeneration is affected by diabetes in experimental animals. Method: Sprague Dawely rat was injected with 45~50 mg/kg of streptozotocin (STZ) to generate an animal model of diabetes. Three months after STZ injection, sciatic nerve (2 cm length) was removed and the same length of nerve segments from STZ-injected animal or from control animal (CTL) was transplanted into STZ-injected animals (STZ-STZ and STZ-CTL respectively). Similarly, sciatic nerve segments from STZ-injected animal or from control animal were grafted into the control animals (CTL-STZ and CTL-CTL respectively). All animals were sacrificed 2 weeks after transplantation. Sciatic nerve sections were prepared and subjected to immunofluorescence staining analysis. Results: Immunofluorescence staining for NF-200 showed that distal elongation of regenerating axons reached 40~80% of proximal neve in both CTL-STZ and CTL-CTL groups. However, distal elongation in both STZ-STZ and STZ-CTL groups were 20~60% of proximal nerve. Furthermore, measurement of axonal regeneration after immuno-staining with SCG10 showed that the scores of distal elongation relative to proximal nerve were 50~90% in CTL-CTL and CTL-STZ groups and 10-60% in STZ-CTL and STZ-STZ. Conclusions: Our data showed that the levels of axonal regeneration were not affected irrespective of whether they were from STZ- or CTL graft, but were greatly reduced when the nerves were transplanted into the STZ host.

• 대한정형외과학회지
• /
• 제52권1호
• /
• pp.33-39
• /
• 2017

목적: 근위 경비골 및 상완골 절제 시 문제점은 알려져 있으나 소전자부 주위 골연골종의 임상상 및 수술 접근법에 대한 연구는 미약하다. 대상 및 방법: 소전자부 주위 골연골종으로 수술한 환자 13명의 증상 및 증상기간, 종양위치, 축상면상 돌출방향, 크기, 수술 접근법 및 장요근 손상 여부, 접근법에 따른 합병증을 후향적으로 분석하였다. 결과: 증상은 운동 및 보행 시 통증이 7예, 저림 및 방사통이 6예였다. 증상기간은 평균 19개월이었고 평균 크기는 120 ml였다. 후내 측으로 돌출된 종괴 5예에서 도달법은 후외방접근법 3예, 전방접근법 1예, 내측접근법 1예였다. 전내측 종괴 4예는 전방접근법으로 절제하였다. 전내측 및 후내측으로 돌출된 2예는 내측접근법 1예, 전방접근법이 1예였다. 후내측 돌출된 2예를 내측접근법으로 수술 후 피질골 결손으로 내고정술을 시행하였다. 후내측 돌출이 심한 1예에서 전방도달법 절제 후 좌골신경마비로 신경탐색술을 시행하였고 6개월 후 자연 회복되었다. 결론: 소전자부 주위 골연골종이 크고 후방돌출이 심하면 좌골신경 압박을 의심해야 한다. 내측도달법은 종양이 작을 때만 유용하고 전내측 돌출 및 경부에 있을 때 전방도달법이 유리하다. 후방돌출이 심한 큰 종괴에서 후방도달법이 신경손상을 최소화할 수 있는 방법이다.

• 생명과학회지
• /
• 제29권2호
• /
• pp.215-222
• /
• 2019

노화, 상해, 유전병 및 산화 스트레스와 같은 다양한 원인으로 인해 근육 위축을 유발한다. 그 동안의 연구에 의하면 열처리 사균체 엔테로코커스 패칼리스(EF-2001)는 항알레르기, 항염증 및 항종양 효과를 보였다. 그러나 항산화 및 항근위축에 대한 효과는 잘 알려져 있지 않고 있다. 본 연구에서는 EF-2001이 근육 위축에 미치는 영향을 연구 하였다. 산화 스트레스에 의한 EF-2001의 세포손상 보호 효과를 확인하기 위해 C2C12 근섬유 아세포는 H2O2로 처리되어, 산화 스트레스를 유도하여 세포 손상을 유발하였다. 그러나 EF-2001 처리로 인해 근세포 손상이 감소됨을 확인 하였다. 우리는 산화스트레스에 의한 EF-2001의 근세포손상의 감소 효과에 대한 메커니즘을 확인하였다. EF-2001는 산화스트레스로 유도된 근세포내의 HSP70 및 SOD1 단백질의 발현을 감소시켰다. 또한, 근세포내에 Atrogin-1/MAFbx 및 MuRF1의 mRNA 수준은 산화 스트레스 조건 하에서 증가하였으나, EF-2001에 의해 감소하였다. 더나아가 근감소를 유도한 좌골신경 절제 모델동물을 통한 근육량을 확인하기 위해 마이크로 CT를 활용해 2차원과 3차원으로 분석하였다. 근육량은 좌골 신경 절제 후에 감소하였고, EF-2001의 경구 투여에 의해 근육량이 회복되었다. 본 결과는 열처리 사균체 엔테로코커스 패칼리스인 EF-2001이 노인들에게 자주 발생하는 근감소증의 예방 및 개선할 수 있는 기능성 식품으로 다양한 분야에서 유용하게 활용 될 수 있음을 시사한다.

• 동의생리병리학회지
• /
• 제22권6호
• /
• pp.1454-1461
• /
• 2008

In oriental medicine, it is known that Eucommiae Cortex (EC) has strengthening and rehabilitative effects on the bone-muscle dysfunction. This study aimed to evaluate the effect of EC on the skeletal muscle atrophy. The muscle atrophy was induced by unilateral transection of the sciatic nerve in Sprague-Dawley rats. EC (water-extract, 170mg/100 g body weight) was treated once a day for 12 days. In this study, the effect of EC examined the muscle weight of hind limb, cross section areas of muscle fibers, fiber type compositions, apoptosis related factors (Bax and Bcl-2). EC reduced muscle atrophy in soleus (SOL), medial gastrocnemius (MGT), extensor digitorum longus, and tibialis posterior significantly in the damaged hind limb. EC increased type-I muscle fibers and decreased type-II muscle fibers significantly in SOL of the damaged hind limb. EC enlarged cross section areas of type-I and type-II muscle fibers significantly in SOL. EC enlarged cross section areas of type-I and type-II muscle fibers significantly in. EC reduced apoptotic nuclei and atrophic muscle fibers in SOL and MGT. EC reduced Bax positive muscle nuclei in SOL and MGT. EC up-regulated Bcl-2 positive muscle fibers in SOL and MGT. These results suggest that EC has an anti-atrophic effect and anti-apoptotic effect against myonuclear apoptosis induced by the peripheral nerve damage.