Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Eucommiae Cortex on Hind Limb Muscle Atrophy of Sciatic Nerve Transectioned Rats

두충이 좌골신경손상 흰쥐의 후지 근육위축에 미치는 영향

  • Cho, Jae-Hun (Department of Neuroscience and Brain Disease, Graduate School of East-West Medicine, Kyung Hee University) ;
  • Kim, Kon-Sik (Department of Neuroscience and Brain Disease, Graduate School of East-West Medicine, Kyung Hee University) ;
  • Cha, Jae-Deog (Department of Anatomy & Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Lee, Hyun-Sam (Department of Anatomy & Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Choi, Hyeon (Hamsoa Pharmaceutical Co. R&D Center) ;
  • Jung, Hyuk-Sang (Department of Anatomy & Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Sohn, Nak-Won (Department of Neuroscience and Brain Disease, Graduate School of East-West Medicine, Kyung Hee University) ;
  • Sohn, Young-Joo (Department of Gynecology, College of Oriental Medicine, Sangji University)
  • 조재헌 (경희대학교 동서의학대학원 신경과학 및 뇌질환) ;
  • 김건식 (경희대학교 동서의학대학원 신경과학 및 뇌질환) ;
  • 차재덕 (경희대학교 한의과대학 해부학교실 & 한의학연구소) ;
  • 이현삼 (경희대학교 한의과대학 해부학교실 & 한의학연구소) ;
  • 최현 ((주)함소아제약 연구개발센터) ;
  • 정혁상 (경희대학교 한의과대학 해부학교실 & 한의학연구소) ;
  • 손낙원 (경희대학교 동서의학대학원 신경과학 및 뇌질환) ;
  • 손영주 (상지대학교 한의과대학 부인과학교실)
  • Published : 2008.12.25
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Abstract

In oriental medicine, it is known that Eucommiae Cortex (EC) has strengthening and rehabilitative effects on the bone-muscle dysfunction. This study aimed to evaluate the effect of EC on the skeletal muscle atrophy. The muscle atrophy was induced by unilateral transection of the sciatic nerve in Sprague-Dawley rats. EC (water-extract, 170mg/100 g body weight) was treated once a day for 12 days. In this study, the effect of EC examined the muscle weight of hind limb, cross section areas of muscle fibers, fiber type compositions, apoptosis related factors (Bax and Bcl-2). EC reduced muscle atrophy in soleus (SOL), medial gastrocnemius (MGT), extensor digitorum longus, and tibialis posterior significantly in the damaged hind limb. EC increased type-I muscle fibers and decreased type-II muscle fibers significantly in SOL of the damaged hind limb. EC enlarged cross section areas of type-I and type-II muscle fibers significantly in SOL. EC enlarged cross section areas of type-I and type-II muscle fibers significantly in. EC reduced apoptotic nuclei and atrophic muscle fibers in SOL and MGT. EC reduced Bax positive muscle nuclei in SOL and MGT. EC up-regulated Bcl-2 positive muscle fibers in SOL and MGT. These results suggest that EC has an anti-atrophic effect and anti-apoptotic effect against myonuclear apoptosis induced by the peripheral nerve damage.

Keywords

References

  1. 김호철. 한약약리학. 집문당, 서울, pp 447-449, 2001.
  2. 홍남두, 노령수, 김종우, 원도희, 김남재, 조보선. 두충나무의 일반약리활성 연구. 생약학회지 19(2):102-110, 1988.
  3. 박선동, 김길훤. 두충의 수치에 따른 실험적 연구. 한의학연구소논문집, 1(1):81-107, 1992.
  4. 김의진, 김선희. 두충과 두충엽이 면역기능에 미치는 영향. 대한본초학지 9(1):99-113, 1994.
  5. Hsieh, C.L., Yen, G.C. Antioxidant actions of du-zhong (Eucommia ulmoides Oliv.) toward oxidative damage in biomolecules. Life Sci 66(15):1387-1400, 2000. https://doi.org/10.1016/S0024-3205(00)00450-1
  6. Lee, M.K., Cho, S.Y., Kim, D.J., Jang, J.Y., Shin, K.H., Park, S.A., Park, E.M., Lee, J.S., Choi, M.S., Lee, J.S., Kim, M.J. Du-zhong (Eucommia ulmoides Oliv.) cortex water extract alters heme biosynthesis and erythrocyte antioxidant defense system in lead-administered rats. J Med Food 8(1):86-92, 2005. https://doi.org/10.1089/jmf.2005.8.86
  7. 오하식, 김호철, 이상인, 안덕균. 두충의 수피와 잎이 난소적출로 유발한 흰쥐의 골다공증에 미치는 영향. 대한본초학회지 10(1):59-68, 1995.
  8. Ha, H., Ho, J., Shin, S., Kim, H., Koo, S., Kim, I.H., Kim, C. Effects of Eucommiae Cortex on osteoblast-like cell proliferation and osteoclast inhibition. Arch Pharm Res 26(11):929-936, 2003. https://doi.org/10.1007/BF02980202
  9. Midrio, M. The denervated muscle: facts and hypotheses. A historical review. Eur J Appl Physiol 98(1):1-21, 2006. https://doi.org/10.1007/s00421-006-0256-z
  10. Siu, P.M., Alway, S.E. Mitochondria-associated apoptotic signalling in denervated rat skeletal muscle. J Physiol 565: 309-323, 2005. https://doi.org/10.1113/jphysiol.2004.081083
  11. Kuschel, R., Yablonka-Reuveni, Z., Bornemann, A. Satellite cells on isolated myofibers from normal and denervated adult rat muscle. J Histochem Cytochem 47(11):1375-1384, 1999. https://doi.org/10.1177/002215549904701104
  12. Serrano, A.L., Perez, M., Lucia, A., Chicharro, J.L., Quiroz-Rothe, E., Rivero, J.L. Immunolabelling, histochemistry and in situ hybridisation in human skeletal muscle fibres to detect myosin heavy chain expression at the protein and mRNA level. J Anat 199: 329-337, 2001. https://doi.org/10.1046/j.1469-7580.2001.19930329.x
  13. Olive, M., Ferrer, I. Bcl-2 and bax immunohistochemistry in denerva- tion-reinnervation and necrosis-regeneration of rat skeletal muscles. Muscle Nerve 23(12):1862-1867, 2000. https://doi.org/10.1002/1097-4598(200012)23:12<1862::AID-MUS10>3.0.CO;2-Q
  14. 이종안, 노승현, 안덕균, 최호영. 두충과 모과의 혼합 전탕액이 흰쥐의 난소적출로 유발한 골다공증에 미치는 영향. 대한본초학회지 16(1):201-206, 2001.
  15. 장준복, 김경직, 최가야, 조정훈, 이경섭. 두충이 난소적출 흰쥐의 골조직 형태 변화에 미치는 영향. 대한한방부인과학회지 16(4):1-14, 2003.
  16. Li, Y., Kamo, S., Metori, K., Koike, K., Che, Q.M., Takahashi, S. The promoting effect of eucommiol from Eucommiae cortex on collagen synthesis. Biol Pharm Bull 23(1):54-59, 2000. https://doi.org/10.1248/bpb.23.54
  17. Ong, V.Y., Tan, B.K. Novel phytoandrogens and lipidic augmenters from Eucommia ulmoides. BMC Complement Altern Med 7: 3, 2007. https://doi.org/10.1186/1472-6882-7-3
  18. 이창근, 이봉교. 두충 및 두충엽이 백서의 고Cholesterol혈증에 미치는 영향. 동서의학, 12(2):17-31, 1987.
  19. 김종배, 박정륭, 전정례, 차명화. 두충잎의 항암성분 분리 및 동정. 한국식품영양과학회지 30(4):732-738, 2001.
  20. Yen, G.C., Hsieh, C.L. Reactive oxygen species scavenging activity of Du-zhong (Eucommia ulmoides oliv.) and its active compounds. J Agric Food Chem 48(8):3431-3436, 2000. https://doi.org/10.1021/jf000150t
  21. Yen, G.C., Hsieh, C.L. Inhibitory effect of Eucommia ulmoides Oliv. on oxidative DNA damage in lymphocytes induced by $H_2O_2$. Teratog Carcinog Mutagen 1: 23-34, 2003.
  22. 하대호, 최유선, 정새진, 이은미. Hydrogen Peroxide로 유도된 혈관독성에 대한 杜沖의 효과. 대한동의생리병리학회지 15(4):595-598, 2001.
  23. Jagoe, R.T., Goldberg, A.L. What do we really know about the ubiquitin proteasome pathway in muscle atrophy? Curr Opin Clin Nutr Metab Care 4: 183-190, 2001. https://doi.org/10.1097/00075197-200105000-00003
  24. Powers, S.K., Kavazis, A.N., McClung, J.M. Oxidative stress and disuse muscle atrophy. J Appl Physiol 102: 2389-2397, 2007. https://doi.org/10.1152/japplphysiol.01202.2006
  25. Kondo, H., Miura, M., Itokawa, Y. Oxidative stress in skeletal muscle atrophied by immobilization. Acta Physiol Scand 142: 527-528, 1991. https://doi.org/10.1111/j.1748-1716.1991.tb09191.x
  26. Kondo, H., Miura, M., Nakagaki, I., Sasaki, S., Itokawa, Y. Trace element movement and oxidative stress in skeletal muscle atrophied by immobilization. Am J Physiol Endocrinol Metab 262: E583-E590, 1992. https://doi.org/10.1152/ajpendo.1992.262.5.E583
  27. Jackson, M.J., Papa, S., Bolanos, J., Bruckdorfer, R., Carlsen, H., Elliott, R.M., Flier, J., Griffiths, H.R., Heales, S., Holst, B., Lorusso, M., Lund, E., Oivind Moskaug, J., Moser, U., Di Paola, M., Polidori, M.C., Signorile, A., Stahl, W., Vina-Ribes, J., Astley, S.B. Antioxidants, reactive oxygen and nitrogen species, gene induction and mitochondrial function. Mol Aspects Med 23: 209-285, 2002. https://doi.org/10.1016/S0098-2997(02)00018-3
  28. Jackson, M.J. Reactive oxygen species and redox-regulation of skeletal muscle adaptations to exercise. Philos Trans R Soc Lond B Biol Sci 360: 2285-2291, 2005. https://doi.org/10.1098/rstb.2005.1773
  29. Kondo, H., Nishino, K., Itokawa, Y. Hydroxyl radical generation in skeletal muscle atrophied by immobilization. FEBS Lett 349: 169-172, 1994. https://doi.org/10.1016/0014-5793(94)00641-5
  30. Du, J., Wang, X., Miereles, C., Bailey, J.L., Debigare, R., Zheng, B., Price, S.R., Mitch, W.E. Activation of caspase-3 is an initial step triggering accelerated muscle proteolysis in catabolic conditions. J Clin Invest 113: 115-123, 2004. https://doi.org/10.1172/JCI18330
  31. Cuschieri, J., Maier, R.V. Mitogen-activated protein kinase (MAPK). Crit Care Med 33: S417-S419, 2005. https://doi.org/10.1097/01.CCM.0000191714.39495.A6
  32. Hunter, R.B., Stevenson, E., Koncarevic, A., Mitchell-Felton, H., Essig, D.A., Kandarian, S.C. Activation of an alternative NF-B pathway in skeletal muscle during disuse atrophy. FASEB J 16: 529-538, 2002. https://doi.org/10.1096/fj.01-0866com
  33. Dupont-Versteegden, E.E., Houle, J.D., Gurley, C.M., Peterson, C.A. Early changes in muscle fiber size and gene expression in response to spinal cord transection and exercise. Am J Physiol Cell Physiol 275: C1124-C1133, 1998. https://doi.org/10.1152/ajpcell.1998.275.4.C1124
  34. Milanic, T., Kunstelj, A., Mars, T., Grubic, Z. Morphometric charac- teristics of myonuclear distribution in the normal and denervated fast rat muscle fiber. Chem Biol Interact 120: 321-326, 1999. https://doi.org/10.1016/S0009-2797(99)00042-3
  35. Borisov, A.B., Carlson, B.M. Cell death in denervated skeletal muscle is distinct from classical apoptosis. Anat Rec 258(3):305-318, 2000. https://doi.org/10.1002/(SICI)1097-0185(20000301)258:3<305::AID-AR10>3.0.CO;2-A
  36. Sandri, M. Apoptotic signaling in skeletal muscle fibers during atrophy. Curr Opin Clin Nutr Metab Care 5: 249-253, 2002. https://doi.org/10.1097/00075197-200205000-00003
  37. Primeau, A.J., Adhihetty, P.J., Hood, D.A. Apoptosis in heart and skeletal muscle. Can J Appl Physiol 27: 349-395, 2002. https://doi.org/10.1139/h02-020
  38. Hyatt, J-P.K., Roy, R.R., Baldwin, K.M., Edgerton, V.R. Nerve activity-independent regulation of skeletal muscle atrophy: role of MyoD and myogenin in satellite cells and myonuclei. Am J Physiol Cell Physiol 285: C1161-C1173, 2003. https://doi.org/10.1152/ajpcell.00128.2003