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http://dx.doi.org/10.5352/JLS.2019.29.2.215

Effect of Heat-Killed Enterococcus faecalis, EF-2001 on C2C12 Myoblast Damage Induced by Oxidative Stress and Muscle Volume Decreased by Sciatic Denervation in C57BL/6 Mice  

Chang, Sang-Jin (Division of Biological Science and Technology, Yonsei University)
Lee, Myung-Hun (Division of Biological Science and Technology, Yonsei University)
Kim, Wan-Joong (Division of Biological Science and Technology, Yonsei University)
Chae, Yuri (Division of Biological Science and Technology, Yonsei University)
Iwasa, Masahiro (Division of Biological Science and Technology, Yonsei University)
Han, Kwon-Il (Division of Biological Science and Technology, Yonsei University)
Kim, Wan-Jae (Research & Development Center, Korea BeRM Co. Ltd.)
Kim, Tack-Joong (Division of Biological Science and Technology, Yonsei University)
Publication Information
Journal of Life Science / v.29, no.2, 2019 , pp. 215-222 More about this Journal
Abstract
Muscle dysfunction may arise from skeletal muscle atrophy caused by aging, injury, oxidative stress, and hereditary disease. Powdered heat-killed Enterococcus faecalis (EF-2001) has anti-allergy, anti-inflammatory, and anti-tumor effects. However, its antioxidant and anti-atrophy effects are poorly characterized. In this study, we examined the effects of EF-2001 on muscle atrophy. To determine the protective effect of EF-2001 on oxidative stress, C2C12 myoblasts were treated with $H_2O_2$ to induce oxidative stress. This induced cell damage, which was reduced by treatment with EF-2001. The mechanism of EF-2001's effect was examined in response to oxidative stress. Treatment with EF-2001 reversed the expression of HSP70 and SOD1 proteins. Also, mRNA levels of Atrogin-1/MAFbx and MuRF1 increased under oxidative stress conditions but decreased following EF-2001 treatment. To evaluate muscle volume, two and three dimensional models of the muscles were analyzed using micro-CT. As expected, muscle volume decreased after sciatic denervation and recovered after oral administration of EF-2001. Therefore, EF-2001 is a candidate for the treatment of muscular atrophy, and future discovery of the additional effects of EF-2001 may yield further applications as a functional food with useful activities in various fields.
Keywords
EF-2001; Heat-killed Enterococcus faecalis; muscle atrophy; oxidative stress; sciatic denervation;
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