• Korean Journal of Weed Science
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• v.18 no.1
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• pp.76-83
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• 1998

Echinochloa species maintained by selling for more than 10 years were classified using random amplified polymorphic DNAs(RAPDs) analysis. Seventy-four decamer of randomly sequence markers were used to classify intraspecific variation irt Echinochloa species. The number of amplification products increased with increasing GC content of the primer in the range between 60% and 70% GC. Single-base substitutions of a primer altered amplification, providing new polymorphisms. The size of amplified DNA was mostly between 0.40kbp and 1.4kbp with the most common bands at 1.1kbp. Echinochloa species were detected with 6 primers which generated 26 polymorphic amplified DNAs. By hierarchical cluster analysis, Echinochloa species collected in Korea were divided into three groups. These results revealed that RAPD markers are useful tools for the determination of genetic variations in Echinochloa species.

• Journal of Food Hygiene and Safety
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• v.27 no.1
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• pp.68-73
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• 2012

In this study, a method was developed using molecular biological technique to distinguish an authenticity of meats for processed meat products. The genes for distinction of species about meats targeted at 12S or 16S genes in mitochondrial DNA and the species-specific primers were designed by that PCR products' size was around 200bp for applying to processed products. The target materials were 10 species of livestock products and it checked whether expected PCR products were created or not by electrophoresis after PCR using species-specific primers. The results of PCR for beef, pork, goat meat, mutton, venison, and horse meat were 131, 138, 168, 144, 191, and 142 bp each. The expected PCR products were confirmed at 281, 186, 174, and 238 bp for chicken, duck, turkeymeat, and ostrich. Also, non-specific PCR products were not detected in similar species by species-specific primers. The method using primers developed in this study confirm to be applicable for composite seasoning including beefs and processed meat products including pork and chicken. Therefore, this method may apply to distinguish an authenticity of meats for various processed products.

• Food preservation and processing industry
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• v.7 no.2
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• pp.33-36
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• 2008

차는 차나무 잎을 가공한 것으로 녹차 등의 비발효차, 우롱차 등의 반발효차, 홍차 등의 발효차로 분류된다. 우롱차, 홍차 등의 발효는 미생물이 관여하는 발효가 아니고 찻잎에 존재하는 효소에 의한 반응을 이용하므로 효소 발효차로 불리운다. 반발효차는 $10{\sim}65%$ 발효도 그리고 발효차는 85% 이상의 발효도 제품으로 통상 정의되나 발효정도를 판단할 수 있는 기준이 설정되어 있지 않아 발효도 100%에 해당되는 위치를 구명하여 발효차 발효정도 판별 기준으로 활용하고자 발효과정 중 일어나는 성분과 색조의 변화를 검토하였다. 적색도(a 값)와 황색도(b 값)는 위조 및 유념 과정에서부터 색을 띠기 시작하여 발효 진행과 함께 증가하여 발효 시작 일정시간 후 최고점에 도달하고 이후 감소를 보였다. 가시영역의 녹차와 홍차의 스펙트럼에서 가장 큰 차이를 보인 420 nm 흡광도 값은 발효 진행과 함께 증가되어 일정시간 후 최고점에 도달하고 이후 감소를 보였다. 찻잎 성분 중 카테킨류는 5종이 검출되었는데 발효 진행과 함께 모두 급격히 감소하였다. 그러나 카페인은 발효 진행과 관계없이 거의 일정 수준을 유지하여 카페인은 효소 발효에 의해 변환되지 않는 성분으로 확인되었다. 4종의 테아플라빈류는 발효 0%에 해당하는 녹차에는 검출되지 않았으나 발효 진행과 함께 생성됨이 확인되었다. 테아플라빈류는 4종이 검출되었는데 이중 생성량이 많은 것은 TF3,3'G와 TF3G 순이었으며 이 성분들은 발효의 진행과 함께 일정시간 후 최고점에 도달하고 이후 감소를 보였다. 발효차 제조과정 중 발효에 의해 생성되는 적색과 황색 그리고 420 nm의 흡광도 값 그리고 발효에 의해 생성되는 TF3,3'G와 TF3G 생성 최고점에 이르는 발효시간이 일치함이 발견되었다. 이 최고점은 발효도 100%에 해당하는 위치로 판단된다.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.30 no.1D
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• pp.45-51
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• 2010

Traffic volume is essential data for traffic control or maintenance and rehabilitation planning. The volume especially with respect to the type of vehicles can facilitate to those road operations. In this research, a method for vehicle classification was developed using skewed sensors which can generate traffic signatures. In order to characterize vehicle types, the method investigates whether the second axle of each vehicle consists of dual tires. The presence of dual tire is determined by the discriminate function obtained from discriminant analysis. The validation using 1,878 vehicles recorded from a highway using a CCTV camera indicated significantly accurate results: 96.92% for class 1, 82.91% for class 3 and 79.13% for class 4.

• Korean Journal Plant Pathology
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• v.3 no.3
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• pp.174-179
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• 1987

Four thousand eight hundred and eighty five isolates of Pyricularia oryzae were obtained from the diseased rice specimens collected from various areas of Korea for race identification during 1978 - 1985. A total of 15 races inculding 6 races pathogenic to Tongil lines, 2 T-races, 5 C-races and 2 N-races was identified using a old Japanese differential set during 1978-1980. Since 1981, number of races identified by a Korean differential set was 18 races which were composed of 11 KI-races pathogenic to either Tongil lines or Japonica cultivars and 8 KJ-races pathogenic to only Japonica cultivars. The prevalent race was $N-2^{+t}$ during 1978-1979 and race KJ-301 since 1980, respectively. Races KI-315a and KI-315b pathogenic to most of Tongil lines were identified in 1983. being widely distributed to date. Races KJ-105 and KJ-201 pathogenic to the cultivars possessing resistance genes Pi-k and Pi-i were prevalent in Gangweon province, whereas race KI-315b was prevalent in Chungbug and Jeonnam districts.

• Journal of Life Science
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• v.17 no.3 s.83
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• pp.402-406
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• 2007

This study was performed to develop the molecular marker for sex determination of hare (Lepus coreanus) distributed in Korea which focused on sexual dimorphism between X and Y chromosomal homologous genes, zinc finger-X (ZFX) and -Y (ZFY). The intron 7 regions of ZFX and ZFY genes exhibited differential amplification patterns between male and female hares. The lengths of intron 7 region of ZFX and ZFY genes were 538 and 233-bp, respectively. Especially, the ZFX intron 7 contained a repetitive sequence identified as member of RNA-mediated transposable elements which was similar to CSINE2 commonly found in the rabbit genome. However, it was not present in intron 7 of ZFY gene. The molecular sex typing by polymerase chain reaction (PCR) was also carried out to determine the sex of hare based on difference in lengths between the intron 7 regions of ZFX and ZFY genes. All DNA samples tested had common band amplified from ZFX. However, the male hare DNAs had two distinct bands which amplified from ZFX and ZFY genes, respectively. The results from ZFX-ZFY PCR sex typing were identical to those from phenotypic investigation and from amplification patterns using male-specific sex determining region Y (SRY) gene as well. Finally, this study suggested that the sexual dimorphism between intron 7 regions of ZFX and ZFY could be useful genetic marker to determine sex of hare.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.39 no.11
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• pp.995-1004
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• 2011

Object X-ray method commonly used for hole search in overset grids requires huge amount of time due to complicated vector calculations to search the cross-points as well as time-consuming hole search algorithm with respect to background grids. Especially, when the grid system is in motion relative to the background, hole points should be searched at every time step, leading to hung computational burden. To cope with this difficulties, this study presents an efficient hole search algorithm mainly designed to reduce hole searching time. To this end, virtual surface with reduced grid points is suggested and logical operators are employed as a classification algorithm instead of complicated vector calculations. In addition, the searching process is further accelerated by designating hole points in a row rather than discriminating hole points with respect to each background grid points. If there exists a relative motion, the present algorithm requires much less time because only the virtual surface needs to be moved at every time step. The hole searching time has been systematically compared for a few selected geometries.

• Korean Journal of Food Science and Technology
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• v.32 no.3
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• pp.538-543
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• 2000

This study was conducted to develop an enzyme-linked immunosorbent assay(ELISA) for the determination of cooked goat meat. Muscle proteins were extracted from goat meat by heating at $98^{\circ}C$ for 15 min. Major thermostable(TS) protein, whose size and pI are 36 and 38 kDa and 4.5 respectively, were purified by DEAE-Sephadex A-50 and Sephadex G-75 column chromatography. The TS protein was immunized into rabbits in order to produce goat specific antibodies. Competitive indirect ELISA(ciELISA) was established by using the anti-TS antibody. The antibody showed high reactivity toward the TS antigen and the boiled goat meat extract but it did not show any reactivities toward extracts of boiled chicken, pork, lamb, and beef. Thus, this ciELISA developed in this study could be applicable to identify goat species from cooked meat.

• Korean Journal of Food Science and Technology
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• v.46 no.3
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• pp.352-357
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• 2014

In this study, 100 consumers (men, 50; women, 50; age group, 20-50 years) rated their overall preferences for 24 Korean raspberry wines by using a 9-point hedonic scale. The analysis of variance was constructed to evaluate the effect of gender, age, and samples on the preference scores of the wine products. Significant differences were observed in overall preferences for the 24 samples; however, no interactions based on preferences by age and gender groups were noted. Cluster analysis was performed to determine sample clustering based on the frequencies from the preference data. Three clusters were obtained; these three clusters were well separated based on the mean overall preference scores for the samples. Discriminant analysis based on the three clusters also confirmed the same grouping of samples with 100% accuracy.

• Food Science of Animal Resources
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• v.27 no.2
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• pp.209-215
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• 2007

In order to develop a sensitive and reliable method for the species-specific molecular markers, PCR-RFLP assay of the mitochondrial DNA(mt DNA) 12S rRNA gene was exploited for the identification of the origin of animal meat species including cattle, pig, sheep, goat, horse, deer, chicken, duck and turkey. A specific primer pairs were designed, based on the nucleotide sequences of mt 12S rRNA gene, for the amplification of the highly conserved region in the gene of the animal species using PCR-RFLP technique. mt DNA was isolated from meat samples followed by DNA amplification using PCR with the specific primers. PCR amplification produced an approximately 455 bp fragment in each of these animal meats. To distinguish pleat species, the PCR amplicons were digested with restriction endonucleases Tsp5091 and MboI, respectively, which generates distinct RFLP profiles. The DNA profiles digested with Tsp5091 allowed the clear discrimination in the mammalian meat species and the DNA profiles digested with MboI in poultry meat species. Therefore, the PCR-RFLP profiles of mt 12S rRNA gene could be very useful to identify the origin of the raw materials in the raw meats as well as the processed meat products.