• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2005.05a
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• pp.195-198
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• 2005

본 연구는 cytochrome B 유전자의 PCR-RFLP 분석기법을 이용하여 다양한 식육자원 및 각종 가공 육제품의 원료육에 대한 정확하고 재현성 높은 축종 및 육종 감별기술을 개발하기 위하여 수행되었다. 국내에서 유통되고 있는 7종류 축종(닭, 양, 돼지, 소, 사슴, 말, 염소)의 육류로부터 cytochrome B 유전자의 특정 염기서열을 포함하는 primer를 설계 제작하여 PCR-RFLP 분석을 실시하였다. 각 축종의 근육조직으로부터 genomic DNA를 추출하고 PCR 증폭 반응을 수행한 후 얻어진 PCR 증폭산물(359 bp)을 두 종류의 제한효소(Hae Ш 및 Hinf I)로 각각 절단한 결과 축종 간 그리고 제한효소 간에 명확한 차이를 보이는 종 특이적인 PCR-RFLP marker를 검출하였다. 따라서, 본 연구에서 개발한 cytochrome B 유전자의 종 특이적 PCR-RFLP marker는 각종 식육 및 가공 육제품의 육종 및 축종 판별에 매우 유용한 DNA marker로 이용될 수 있을 것이다.

• Korean Journal of Food Science and Technology
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• v.44 no.4
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• pp.484-487
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• 2012

A discrimination technique for domestic and imported Scutellaria baicalensis was developed using an energy dispersive X-ray fluorescence spectrometer (ED-XRF). Mineral content ratios, of a total of 43 species, including P, S, Cl, K, Ca, Mn, Fe, Cu, and Zn, were measured among 204 samples. Macro element content ratios and trace element content ratios were determined using the standardless fundamental parameters (SLFP) analysis. Inorganic element ratios of P, S, K, Ca, Cl, Mn, and Fe were significantly different between domestic and imported samples. The result from the canonical discriminant analysis showed that the accuracy of geographical origin discrimination was 95.15%; the correlation coefficient was 0.888. It was concluded that this technique could be used as a useful method in discriminating the geographical origins between domestic and imported Scutellaria baicalensis.

• Analytical Science and Technology
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• v.34 no.3
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• pp.134-141
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• 2021

In this study, the fatty acid (FA) composition of commercial sesame oils (n = 62) was investigated using gas chromatography with flame ionization detector (GC-FID). Multivariate statistical techniques, including principal component analysis (PCA) and linear discriminant analysis (LDA), were applied to the chromatographic data of the FAs to discriminate the geographical origin of sesame oils. A statistically significant difference was observed in the content of C16:0, C18:0, C18:1, and C18:2 between domestic and imported sesame oils. A satisfactory recovery rate of 82.8-100.2 % was achieved for C16:0, C18:0, C18:1, C18:2, and C18:3. The correlation of C16:0, C18:1, and C18:2 in domestic sesame oils showed opposite trends compared to imported oils. The PCA plot demonstrated that sesame oils were clustered in distinct groups according to their origin. LDA was used to predict sesame oil samples in one of the two groups. C16:0 (Wilks λ = 0.361) and C18:1 (Wilks λ = 0.637) demonstrated the highest discriminant power for classifying the origin of the samples. The correct prediction rates were 88.9 % and 100 % for the domestic and imported samples, respectively. Further, 60 of the 62 sesame oil samples (96.8 %) were correctly classified, indicating that this approach can be used as a valuable tool to predict and classify the geographical origin of sesame oils.

• Korean Journal of Plant Resources
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• v.12 no.4
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• pp.324-339
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• 1999

Morphological reinvestigation, external characters and discriminant functional analysis(44 qualitative characters) were examined on 6 taxa of Korean Ophelia, including 5 taxa distributed in south Korea, and one taxon considered to be the variation type of Ophelia wilfordi in order to clarify the limit of intersection and interspecies. And to establish the taxonomic position. One taxon distributed in north Korea was included in the description of species by observation of herbarium specimen of the University of Tokyo in Japan. The two sections were successfully distinguished by internal structure of ovary, morphology of nectary, number of corolla lobe and calyx lobe, and species were also distinguished by morphology of cauline leaf, and color and spot of corolla, respectively. The variation type of Ophelia wilfordi was not distinguished with other species except for absent or present of purple spot in corolla lobe. The results of the discriminal functional analysis indicated that characters of corolla were the most important qualitative characters to distinguish the Ophelia taxa, and morphology of seed and seed coat was useful characters to distinguish taxa higher than species. Therefore the difficult problems of identification of species were successfully solved, and the taxonomic position in intrageneric level was clarified on Korean Ophelia.

• Journal of Korea Society of Industrial Information Systems
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• v.29 no.2
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• pp.15-25
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• 2024

For effective analysis of animal ecosystems, technology that can automatically identify the current status of animal habitats is crucial. Specifically, animal sound classification, which identifies species based on their sounds, is gaining great attention where video-based discrimination is impractical. Traditional studies have relied on a single deep learning model to classify animal sounds. However, sounds collected in outdoor settings often include substantial background noise, complicating the task for a single model. In addition, data imbalance among species may lead to biased model training. To address these challenges, in this paper, we propose an animal sound classification scheme that combines predictions from multiple models using Focal Loss, which adjusts penalties based on class data volume. Experiments on public datasets have demonstrated that our scheme can improve recall by up to 22.6% compared to an average of single models.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.66-66
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• 2022

속성수로 활용되는 버드나무속 식물들은 생식기관과 영양기관의 성장 시기가 달라 형태적 특성 평가를 위해 수년간의 조사 기간이 요구된다. 따라서 바이오매스 우수 버드나무속 교잡종 육성의 성공 여부를 조기 판별하기 위한 식별 기술이 필요하다. DNA 마커는 식물의 생장단계와 관련 없이 탐색할 수 있으며 환경에 영향을 받지 않는 장점이 있다. 식물의 계통 분류 시 주로 사용되는 엽록체 DNA는 유전자 염기서열의 변이가 비교적 크지 않은 장점이 있으나 대부분의 활엽수에서 모계를 통해 유전되는 특징이 있다. 하지만 종간 교잡종의 식별은 각각의 부모종과 구분할 수 있어야 하므로 본 연구는 엽록체 DNA가 아닌 핵 DNA를 대상으로 분석하였다. 본 연구의 목적은 호랑버들을 암나무로 갯버들을 수나무로 인공교배하여 육성된 종간 교잡종을 식별하는 핵 DNA 마커를 탐색하는 것이다. 이를 위해 버드나무속에서 개발된 총 35개의 nSSR (nuclear Simple Sequence Repeat) 마커를 대상으로 호랑버들과 갯버들, 종간 교잡종의 식별 가능성을 평가하였다. 분석 결과 호랑버들과 갯버들, 종간 교잡종 간 차이를 나타내는 2개의 핵 DNA 마커를 선발하였다. 따라서 선발된 핵 DNA 마커를 활용하여 호랑버들과 갯버들, 종간 교잡종의 조기 식별에 활용이 가능할 것으로 사료된다.

• Korean Security Journal
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• no.51
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• pp.103-124
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• 2017

The purpose of this study is to analyze regional clusters and discrimination on size and sector of private security in national 16 areas in 2015. The results of the study using hierarchical cluster analysis and discriminant analysis are as follows. First, we can classify national 16 areas into 3 clusters, such as Large Area(1), Medium Area(2), and Small Area(3) according to the size of private security. The discriminant functions D for each cluster is as follows: (1) Large Area(1) = -383.981 + (.108 ${\times}$ number of security companies) + (.016 ${\times}$ number of guards), (2) Medium Area(2) = -35.570 + (.029 ${\times}$ number of security companies) + (.005 ${\times}$ number of guards), and (3) Small Area(3) = -5.381 + (.012 ${\times}$ number of security companies) + (.002 ${\times}$ number of guards). Second, we can classify national 16 areas into 3 clusters, such as Large Area(1), Medium Area(2), and Small Area(3) according to the sector of private security. The discriminant function D for each cluster is as follows: (1) Large Area(1) = -2224.402 + (-.562 ${\times}$ facilities security) + (1.245 ${\times}$ protection security) + (171.142 ${\times}$ convoy security) + (-2.722 ${\times}$ electronic security) + (2.020 ${\times}$ special security), (2) Medium Area(2) = -4.762 + (.052 ${\times}$ facilities security) + (.063 ${\times}$ protection security) + (-3.819 ${\times}$ convoy security) + (.110 ${\times}$ electronic security) + (-.165 ${\times}$ special security), and (3) Small Area(3) = -125.742 + (-.009 ${\times}$ facilities security) + (.432 ${\times}$ protection security) + (5.748 ${\times}$ convoy security) + (5.530 ${\times}$ electronic security) + (-.901 ${\times}$ special security).

• Animal Systematics, Evolution and Diversity
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• no.nspc2
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• pp.243-250
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• 1988

The wing morphometric characters of P.elaeagni complex feeding on the genus Elaeagnus plants was analysed by the multivariate methods using clustering of generalized distance and discriminant analysis. On the clustering of the species, the effect of sexual differences, seasonal variation and geographic population sensitively appeared . However, four species of this group was precicely divided by the discriminant analysis.

• Korean Journal of Agricultural Science
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• v.26 no.2
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• pp.33-38
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• 1999

This study was carried out to analyze the genetic polymorphisms of genomic DNA of blood and meat for conservation of the genetic resources and genetic improvement of Korean Native goat. The genetic identification between Korean Native goat and imported goat was examined using RAPD(random amplified polymorphisms DNAs) analysis with 30 Korean Native goat, 10 hybrid, 10 imported goat. 10 Korean native goat meat and 10 imported goat meat. The results obtained from this study were summarized as follows: 1. Genomic DNA from Korean native goat, hybrid and imported goat could be obtained above about 23kb size using 0.5% agarose gel electrophoresis and the ratio of optical density at 260nm to that at 280nm was between 1.7 and 2.0 using UV spectrophtometer instrument. 2. In the results of the gene identification between Korean Native goat and hybrid, and imported goat using RAPD methods with random primer of 110 kinds, only Korean native goat showed a specific band at about 369bp using a random primer OPO-19 (5'-CAA ACG TCG G-3'), but imported goat and hybrid not showed. 3. Also, in the results of the gene identification between Korean Native goat meat and imported goat meat using RAPD methods with random primer, Korean native goat only showed a specific band at about 369bp using a random primer No. 19(5'-CAA ACG TCG G-3'), but imported goat not showed.

• Journal of Food Hygiene and Safety
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• v.36 no.4
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• pp.331-341
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• 2021

DNA barcode sequences of commercial seafood products, which are difficult to morphologically discriminate, were analyzed to determine cases of food fraud. The gene sequences were analyzed by amplifying the COX I (cytochrome C oxidase subunit I) gene region of mitochondrial DNA, which is mainly used for species identification. The DNA barcode sequences were compared with the gene sequence of each fish registered in the US National Center for Biotechnology. A total of 46 processed seafood products (12 Pagrus majo, 4 Oplegnathus fasciatus, 7 Dentex tumifrons, 2 Acanthopagrus schlegelii, 7 Oreochromis niloticus, 6 Branchiostegus japonicus, 8 Branchiostegus albus) were investigated. Having DNA sequence identity of more than 97% was judged as the same species. As a result of this study, no cases of forgery and alteration were detected. However, some disparities in the commercial names used in local markets and the standard names given in the Korea Food Code were found, which may cause confusion for consumers. It is therefore suggested that the standard name or scientific name be displayed on seafood product labels.