Browse > Article
http://dx.doi.org/10.13103/JFHS.2021.36.4.331

Food Fraud Monitoring of Raw Materials for Commercial Seafood Products Using DNA Barcode Information  

Park, Eun-Ji (Department of Food science and Technology, Suncheon National University)
Kang, Ju-Yeong (Department of Food science and Technology, Suncheon National University)
Lee, Han-Cheol (Department of Food science and Technology, Suncheon National University)
Park, Min-Ji (Department of Food science and Technology, Suncheon National University)
Yang, Ji-Young (Department of Food Science and Technology, Pukyong National University)
Shin, Ji-Young (Department of Food Science and Technology, Pukyong National University)
Kim, Gun-Do (Department of Microbioligy Pukyong National University)
Kim, Jong-Oh (Institute of Marine Biotechnology, Pukyong National University)
Seo, Yong-Bae (Institute of Marine Biotechnology, Pukyong National University)
Kim, Jung-Beom (Department of Food science and Technology, Suncheon National University)
Publication Information
Journal of Food Hygiene and Safety / v.36, no.4, 2021 , pp. 331-341 More about this Journal
Abstract
DNA barcode sequences of commercial seafood products, which are difficult to morphologically discriminate, were analyzed to determine cases of food fraud. The gene sequences were analyzed by amplifying the COX I (cytochrome C oxidase subunit I) gene region of mitochondrial DNA, which is mainly used for species identification. The DNA barcode sequences were compared with the gene sequence of each fish registered in the US National Center for Biotechnology. A total of 46 processed seafood products (12 Pagrus majo, 4 Oplegnathus fasciatus, 7 Dentex tumifrons, 2 Acanthopagrus schlegelii, 7 Oreochromis niloticus, 6 Branchiostegus japonicus, 8 Branchiostegus albus) were investigated. Having DNA sequence identity of more than 97% was judged as the same species. As a result of this study, no cases of forgery and alteration were detected. However, some disparities in the commercial names used in local markets and the standard names given in the Korea Food Code were found, which may cause confusion for consumers. It is therefore suggested that the standard name or scientific name be displayed on seafood product labels.
Keywords
Sea Bream; Malacanthidae; DNA barcode; Food fraud; Species identification;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Oh, C.H., Kim, B.H., Ahn, S.D., Kim, K.S., Development of physicochemical methods for the characterization of adulterated foods. Food Sci. Ind., 47, 20-45 (2014).   DOI
2 Schellenberg, A., Chmielus, S., Schlicht, C., Camin, F., Perini, M., Bontempo, L., Heinrich, K., Kelly, S.D., Rossmann, A., Thomas, F., Jamin, E., Horacek, M., Multielement stable isotope ratio (H, C, N, S) of honey from different european regions, Food Chem., 121, 770-777 (2010).   DOI
3 Civera, T., Species identification and safety of fish products. Vet. Res. Commun., 27, 481-489 (2003).   DOI
4 Moretti, V.M., Truchini, G.M., Bellagamba, F., Caprino, F., Traceability issues in fishery and aquaculture products. Vet. Res. Commun., 7, 497-505 (2003).
5 Chung, I.Y., Seo, Y.B., Yang, J.Y., Kim, G.D., Development and validation of real-time PCR to determine Branchiostegus japonicus and B. albus species based on mitochondrial DNA. J. Life Sci., 27, 1331-1339 (2017).   DOI
6 Hebert, P.D.N., Gregory, T.R., The promise of DNA barcoding for taxonomy. Syst. Biol., 54, 852-859 (2005).   DOI
7 Folmer, O., Black, M., Hoeh, W., Lutz, R., Vrijenhoek, R., DNA primers for amplification of mitochondrial cytochrome C oxidase subunit from diverse metazoan invertebrates. Mol. Mar. Biol. Biotechnol., 3, 294-299 (1994).
8 Kim, B.H., Lee, J.W., Lee, Y., Lee, H.S., Jang, Y.A., Kim, C.I., Food and nutrient consumption patterns of the Korean adult population by income level - 2001 National health and nutrition survey. Korean J. Community Nutr., 10, 952-962 (2005).
9 Statistics Korea, (2020, October 7). Seafood consumption (per person per year) and self-sufficiency rate. Retrieved from http://www.index.go.kr/potal/main/EachDtlPageDetail.do?idx_cd=1317
10 Park, J.A., Analysis on the satisfaction with selection attributes of processed seafood : Focusing on differences by gender, ages, and household types. JFMSE, 30, 1040-1050 (2018).   DOI
11 Warner, K., Roberts, W., Mustain, P., Lowell, B., Swain, M., Casting a wider net: More action needed to stop seafood fraud in the United States. OCEANA, DOI: 10.31230/osf.io/sbm8h (2019).
12 Kim, H.Y., Hong, K.H., Hong, J.H., Kim, D.S., Han, S.B., Lee, E.J., Lee, J.S., Kang, K.J., Chung, H.W., Song, K.H., Park, J.S., Kwon, Y.K., Jang, Y.M., Shin, I.S., Lee, C.K., Ha, S.C., Jo, J.S., Park, H.K., Studies on the separation and discrimination of the natural yellow pigment on croaker. Korean J. Food Sci. Technol. 34, 762-769 (2002).
13 Kim, K.H., Kim, Y.S., Kim, M.R., Lee, H.Y., Jung, Y.K., Lee, J.H., Chang, H.S., Park, Y.C., Kim, S.Y., Choi, J.D., Jang Y.M., Development of species-specific primer to determine the authenticity of vegetable raw materials in food. Food Eng. Prog. 18, 419- 426 (2014).   DOI
14 Kim, M.R., Kwon, K.S., Jung, Y.K., Kang, T.S., A rapid realtime PCR method to differentiate between mottled skate (Beringraja pulchra) and other skate and ray species. Food Chem., 255, 112-119 (2018).   DOI
15 Kim, N.Y.S., Yang, J.Y., Kim, J.B., Development and validation of a PCR method to discriminate between Branchiostegus japonicus and Branchiostegus albus. Korean J. Food Sci. Technol., 51, 295-299 (2019).   DOI
16 Michael, T.M., Michael, B., Gregory, T.R., Vogler, A. P., DNA-based species delineation in tropical beetles using mitochondrial and nuclear markers. Philos. Trans. R. Soc. B Biol. Sci., 360, 1925-1933 (2005).   DOI
17 Kim, K.H., Kim, Y.S., Kim, M.R., Lee, H.Y., Jung, Y.K., Lee, J.H., Chang, H.S., Park, Y.C., Kim, S.Y., Choi, J.D., Jang, Y.M., Development of species-specific primer to determine the authenticity of vegetable raw materials in food. Food Eng. Prog., 18, 419- 426 (2014).   DOI
18 Kang, T.S., Basic principles for developing real-time PCR methods used in food analysis: a review. Trends Food Sci. Technol., 91, 574-585 (2019).   DOI
19 Kang, T.S., Development of four PCR-based methods to differentiate tilefish species (Branchiostegus japonicus and B. albus). Food Chem., 271, 1-8 (2019).   DOI
20 Handy, S.M., Deeds, J.R., Ivanova, N.V., Hebert, P.D., Hanner, R., Ormos, A., Weigt, L.A., Moore, M.M., Hellberg, R.S., Yancy, H.F., Single laboratory validated method for DNA-barcoding for the species identification of fish. J. AOAC Int., 94, 201-210 (2011).   DOI
21 Jin, S.Y., Im, Y.J., Choi, J.H., Jeong, J.M., Nam, K.M., Kim, D.G., Choi, Y.J., Baeck, G.W., Maturation and spawning of the red seabream (Pagrus major) in the south sea of Korea. Korean J. Fish. Aquat. Sci., 53, 43-49 (2020).   DOI
22 Fabric, T., Celia, M., Catherine, H., Food and forensic molecular identification: update and challenges, Trends Biotechnol., 23, 359-366. (2005).   DOI
23 Son, M.Y., Choi, K.M., Joo, M.S., Park, C.I., Molecular characterization and expression analysis of the interleukin 7 receptor alpha chain (IL-7Rα) gene from red seabream (Pagrus major). JFMSE, 32, 560-569 (2020).   DOI
24 Georgina, L.H., Valerie, J.R., Susan, E.P., Hartmut, R., Javier, Q., Rodrego, V., Manuel, R.M., Carmen, G.S., Ricardo, I.P.M., Ana, T.S., Carla, R., Development of a DNA-based method aimed at identifying the fish species present in food products. J. Agric. Food Chem., 49, 1175-1179 (2001).   DOI
25 Mackie, I., Craig, A., Etienne, M., Jerome, M., Fleurence, J., Jessen, F., Smelt, A., Kruijt, A., Yman, I.M., Ferm, M., Martinez, I., Martin, R.P., Pineiro, C., Rehbein, H., Kundiger, R., Species identification of smoked and gravid fish products by sodium dodecylsulphate polyacrylamide gel electrophoresis, urea isoelectric focusing and native isoelectric focusing: a collaborative study. Food Chem. 71, 1-7 (2000).   DOI
26 Yu, Y.C., Hong, Y.W., Kim, J.J., Kim, H.S., Kang, T.S., Monitoring of commercial cephalopod products sold on the South Korea market using DNA barcode information. J. Food Hyg. Saf., 34, 502-507 (2019)   DOI
27 Jenuth, J.P., Perterson, A.C., Fu, K., Shoubridge, E.A., Random genetic drift in the female germline explains the rapid segregation of mammalian mitochondrial DNA. Nat. Genet., 14, 146-151 (1996).   DOI
28 Park, Y.C., Jung, Y. H., Kim, M.R., Shin, J.H., Kim, K.H., Lee, J.H., Cho, T.Y., Lee, H.J., Lee, S.J., Han, S.B., Development of detection method for Niphon spinosus, Epinephelus bruneus, and Epinephelus septemfasciatus using 16S rRNA gene. Korean J. Food Sci. Technol., 45, 1-7 (2013).   DOI
29 Kim, J.C., Kang I.K., Kim D.S., Lee J.H., The fishery and fishing ground environment for red horsehead (Branchiostegus japonicus) on the adjacent seas of Jeju Island. J. Korean Soc. Fish. Ocean Technol., 42, 19-29 (2006).   DOI
30 Chung, J.Y., Jung, Y.M., A study on comparative analysis of food characteristics of sea bream and similar species. CSHR, 23, 159-168 (2017).
31 Kang, T.S., Monitoring of commercial products sold on sushi buffet restaurants in South Korea using DNA barcode information. J. Food Hyg. Saf., 35, 45-50 (2020)   DOI
32 Choi, I.S., Shin, J.Y., Yang, J.Y., Development and validation of multiplex PCR method for the identification of Pagrus major and Sciaenops ocellatus. J. Food Hyg. Saf., 35, 561-566 (2020).   DOI
33 Armani, A., Guardone, L., Castigliego, L., D'Amico, P., Messina, A., Malandra, R., Gianfaldoni D., Guidi, A., DNA and Mini-DNA barcoding for the identification of Porgies species (family Sparidae) of commercial interest on the international market. Food Control, 50, 589-596 (2015).   DOI
34 Iwatsuki, Y., Akazaki, M., Taniguchi, N., Review of the species of the genus Dentex (Perciformes: Sparidae) in the western Pacific defined as the D. hypselosomus complex with the description of a new species, Dentex abei and a redescription of Evynnis tumifrons. Bull. Natl. Mus. Nat. Sci. Ser. A, Suppl., 1, 29-49 (2007).
35 Kim, Y.U., Ryu, J.H. Taxonomic revision of the Genus Branchiostegus (Perciformes, Pisces) from the adjacent waters of Korea. Korean Journal of Ichthyology, 10, 40-48 (1998).
36 Moon, D.Y., Jeong, H.G., Myoung, J.G., Choi, J.H., Kwun, H.J., Back, J.W., Hong, S.Y., Kim, S.Y. Fish Species Collected by the Fish Collection Project from the Southern Sea of Korea during 2010-2012. Korean J. Fish. Aquat. Sci., 48, 507-528 (2015).   DOI
37 Kang, T.S. Rapid and simple identification of two closelyrelated snow crabs (Chionoecetes opilio and C. japonicus) by direct triplex PCR. LWT-Food Sci. Technol., 99, 562-567 (2019).   DOI