• Korean Journal of Microbiology
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• v.45 no.4
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• pp.368-376
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• 2009

A red pigment-producing bacterial strain was isolated from sediment sample of the East China Sea. The isolate was identified by analysis based on 16S rDNA sequence and morphological, physiological properties, biochemical characteristics and fatty acid composition. Phylogenetic analysis based on 16S rDNA sequence showed that isolate represent a phyletic lineage within the genus Zooshikella, and this strain was most closely related to Zooshikella ganghwensis KCTC $12044^T$ (AY130994) (99.79%). The strain was Gram-negative, aerobic and required NaCl at 0.5~8.0% for growth. The predominant cellular fatty acids were saturated and monounsaturated straight-chain fatty acids. Consequently, this strain was identified as a member of the genus Zooshikella and designated as Zooshikella sp. JE-34. The pigment showed characteristics similar to prodigiosin, a well-known red pigment previously detected in Serratia marcescens. The antimicrobial activity of Zooshikella sp. JE-34 bacterial pigment was tested against 18 microorganisms, which were fish and human pathogens. The Zooshikella sp. JE-34 red pigment showed high antimicrobial activity against Streptococcus iniae, S. parauberis, S. mutans, Staphylococcus aureus, and Propionibacterium acnes.

• Korean Chemical Engineering Research
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• v.48 no.2
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• pp.244-252
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• 2010

The objective of this study is to assess feasibility of simultaneous analysis for trace multi-components odorous and volatile organic compounds using a Triple-bed adsorbent tube with a thermal desorber and GC-MS. Triple-bed adsorbent tube is 3 bed packed Tenax-TA with small amount of Carbopack B and Carbosieve SIII in order of adsorption strength in a tube. The operating conditions of GC-MS was possibly able to and effectively detect high volatile and low molecular weight compounds at the mass range of 20~350 m/z using a below impurity 1ppm of Helium carrier gas, of which quantitatively analyzed by target ion extracts. According to the experiment, $C_1{\sim}C_5$ of 14 components; sulfur containing compounds(2), ketones(2), alcohols(4) and aldehydes(6) were simultaneously analyzed with recoveries of 99%, and good repeatability and linearity. High volatile and low molecular weight compounds such as methyl alcohol and acetaldehyde can be safely quantified with high recovery at a condition of 50mL/min of flow rate, below 2L of adsorption volume, and 45% of relative humidity. Target ion extract can also simultaneously quantify multicomponents with odorous and volatile organic compounds in an occasion of piled up two peaks.

• Applied Microscopy
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• v.26 no.1
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• pp.67-77
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• 1996

Pancreases obtained from native Korean goats were used, and examined by immunoelectron microscopy using several antisera. Five types cells, glucagon (A), insulin (B), somatostatin (D), and pancreatic polypeptide (PP-I and PP-II) cells, were identified in the pancreatic islets. The morphologies of A, B, and D cells corresponded to the typical charateristics described in previous reports on other mammals. Serotonin immunoreactivity was observed in the D cells on the basis of the granular profiles. Two types of PP cells could be distinguished on the basis of the granular profile: the first type was formed by round, homogeneous secretory granules ($220{\sim}400nm$) having a narrow halo between the dense core and limiting membrane, while the other type consisted of cells whose secretory granules ($240{\sim}440\;nm$ in the major axis, $150{\sim}200nm$ in the minor axis) were pleomorphic, having a dense core and a closely fitting limiting membrane. From these results, we suggest that the pancreatic islets of the native Korean goat consist of five types of endocrine cells, A, B, D, PP-I and PP-II cells. Among these, PP-I type cells may correspond to the classical PP of other mammalian pancreases, while PP-II type cells may correspond to the enterochromaffin cells in other species.

• Applied Biological Chemistry
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• v.50 no.1
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• pp.57-62
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• 2007

The fruiting body of Phellinus linteus was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2$O. The repeated silica gel and ODS column chromatographies of the EtOAc fraction led to isolation of four sterols. From the result of spectral data including NMR, MS and IR, the chemical structures of the sterols were determined as ergosta-7,24(28)-dien-3${\beta}$-ol (episterol, 1), 5${\alpha}$,8${\alpha}$-epidioxyergosta-6,9(11),22-trien-3${\beta}$-ol (dehydrop-eroxyergosterol, 2), 5${\alpha}$,8${\alpha}$-epidioxyergosta-6,22-dien-3${\beta}$-ol (ergoterol peroxide, 3), and $3{\beta}$,$5{\alpha}$-dihydroxy-6${\beta}$-methoxyergosta-7,22-diene (6-O-methylcerevisterol, 4). The ergosterols have been first isolated from this mushroom in this study.

• The Korean Journal of Mycology
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• v.46 no.2
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• pp.98-104
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• 2018

The goal of this study was to elucidate wild yeast diversity of the midstream region of the Geumgang river, Gongju, Chungnam province, Korea. Fifty strains of 24 species of wild yeasts were isolated from 45 water and soil samples among which Cryptococcus spp. (11 strains), Cryptococcus magnus (7 strains), Rhodotorula spp. (9 strains), and Hanseniaspora spp. (6 strains) were dominant. Four species, Candida chauliodes WJSF 0201, Candida oleophila WJSF 0202, Candida catenulata WJSF 0203, and Candida jaroonii WJSF 0204, represented newly recorded yeasts in Korea. All of these unrecorded yeasts were oval in shape, formed ascospores and pseudomycelium, and grew in vitamin-free medium. Candida oleophila WJSF 0202 was thermophilic which can grow at $37^{\circ}C$.

• Research in Plant Disease
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• v.18 no.2
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• pp.133-138
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• 2012

In November 2009, a powdery mildew on glossy abelia (Abelia ${\times}$ grandiflora) was found in Seogwipo, Jeju Island, Korea. Further survey in the southern part of Korea, e.g., Jeju, Busan, and Tongyeong confirmed occurrence of the disease. White colonies were present on leaves, young stems, and flowers, detracting from their beauty in landscape plantings. Severely infected lesions were discolored to red-purplish. Based on the morphological characteristics and analysis of rDNA, the fungus associated with the symptoms was identified as Erysiphe abeliicola U. Braun & S. Takam. This work provides the morphological feature of its anamorph for the first time, which is characterized by having multi-lobed hyphal appressoria and short foot-cells of conidiophores. Morphological characteristics of mature chasmothecia were consistent with the previous Japanese record of this species. The sequence of internal transcribed spacer region of ribosomal DNA obtained from a Korean sample showed that this species places in the section Microsphaera of the genus Erysiphe in phylogenetic position, corresponding with the classical taxonomy. This is the first report of E. abeliicola and its host plant in Korea. The host plant A. ${\times}$ grandiflora is newly listed in the host range of E. abeliicola.

• Research in Plant Disease
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• v.14 no.1
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• pp.71-75
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• 2008

An isolate of Peanut stunt virus (PSV), named as Tr-PSV, was isolated from white clover (Trifolium repens L) showing mosaic symptom. Tr-PSV systemically infected all plants tested in the Nicotiana spp. and induced local lesions on inoculated leaves of Chenopodium amaranticolor. However, Tr-PSV induced typical mosaic symptoms as ER-PSV on Vigna unguiculata 5 to 6 days after inoculation, while Fny-CMV used as a control virus of Cucumovirus produced local lesions on inoculated leaves. In dsRNA analysis, Tr-PSV consisted of four dsRNAs, but satellite RNA was not detected. The cDNA of coat protein gene of Tr-PSV was amplified by RT-PCR using a Cucumovirus-specific single pair primers that designed to amplify a DNA fragment of approximately 950 bp. By restriction mapping analysis using RFLP of the RT-PCR products and by serological properties of gel diffusion test, Tr-PSV belongs to a typical member of PSV subgroup I. This is the first report on the occurrence of PSV in white clover in Korea.

• Korean Journal of Pharmacognosy
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• v.36 no.3 s.142
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• pp.164-170
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• 2005

In the present study, we investigated the mineral and metabolite composition and antioxidant activities in the flower, stem and root of Aster ciliosus Kitamura in order to detect biologically active substances and develop novel functional resources. The mineral and metabolite compositions differ significantly among the organ of Aster ciliosus Kitamura. The flower is composed of K as 827 mg/100g. while the stem and root are composed of Na as 654.64mg/100g and 771.70mg/100g, respectively. Contents of unsaturated fatty acids, such as oleic, linoleic, and linolenic acid, were higher than those of saturated fatty acid, such as palmitic acid in flower. The contests of phenolic compounds were measured at 285 nm using different solvents: 2.53 and 2.03 optical density (OD) in chloroform and 1.864 and 1.715 in ethanol extracts for the stem and root, respectively. Antioxidant activity was measured in 75% ethanol extracts: 41.3, 42.0 and $45.2\;{\mu}g/ml$ in stem, root and flower at $IC_{50}$ value, respectively. These results suggest that extracts from Aster ciliosus Kitamura can be potentially used as novel resources for antioxidant and biological active substances.

• Journal of Life Science
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• v.26 no.7
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• pp.819-825
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• 2016

The present study was done to assess the diversity of the bacterial community associated with Ulva pertusa collected from Jeju Island using Restriction Fragment Length Polymorphism (RFLP) marker analysis. For RFLP analysis, a total of 145 bacterial strains associated with Ulva pertusa were screened and cultivated using Marine agar and R2A agar. The PCR amplicons of the 16S rRNA gene from all the isolated strains were digested with HaeIII and RsaI restriction enzymes and then classified into different groups according to their restriction patterns. Strains selected based on the RFLP patterns showed more than 91% 16S rRNA gene sequence similarity when compared with known bacterial species, which include 4 phyla - proteobacteria (alpha-proteobacteria, beta-proteobacteria, gamma-proteobacteria - 63%), firmicutes (11%), actinobacteria (4%), bacteroidetes (22%)–as well as 7 classes (actinobacteria, flavobacteriia, cytophagia, bacilli, α-proteobacteria, γ-proteobacteria, β-proteobacteria), 13 orders, 18 families, and 27 genera. These results confirmed a wide diversity of bacterial communities as contrasted with other regions. The newly isolated 10 strains, which show 16S rRNA sequence similarity of <97% compared to previously identified bacteria, could be noble species. Further experiments, such as morphological, physiological, and biochemical classification, are necessary to confirm the novelty of the newly isolated 10 strains.

• Korean Journal of Food Science and Technology
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• v.48 no.5
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• pp.445-453
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• 2016

Eighty-four strains of lactic acid bacteria were isolated from Korean fermented foods for the production of fermented apple juice. Among these strains, the JBE245 strain that showed rapid growth and food functionality was selected and identified as Lactobacillus plantarum. This strain reached the stationary phase after 24 h fermentation at $30^{\circ}C$ with $1.5{\times}10^8$ colony forming unit (CFU)/mL of viable cells, and maintained its viability levels even after 14 days of storage. During fermentation, the ${\alpha}-glucosidase$ inhibitory activity (40.4%), total polyphenol content (583.6 mg gallic acid equivalent (GAE)/mL), and 2,2-diphenyl-l-picryl-hydrazyl hydrate (DPPH) radical scavenging activity (52%) were increased. As judged by a sensory test, the overall preference for the fermented juice (4.22) was comparable to that for the unfermented juice (4.72), indicating that fermentation does not significantly affect the sensory characteristics of apple juice. Consequently, the fermented beverage containing L. plantarum JBE245 and apple juice is a promising functional health food.