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http://dx.doi.org/10.5352/JLS.2016.26.7.819

Phylogenetic Diversity and Community Analysis of Marine Bacteria Associated with Ulva pertusa  

Choi, Ha-Ri (Department of Marine Biomedical Sciences, Jeju National University)
Park, So-Hyun (Department of Marine Biomedical Sciences, Jeju National University)
Kim, Dong-Hwi (Department of Marine Biomedical Sciences, Jeju National University)
Kim, Ji-Young (Research institute for basic science, Jeju National University)
Heo, Moon-Soo (Department of Marine Biomedical Sciences, Jeju National University)
Publication Information
Journal of Life Science / v.26, no.7, 2016 , pp. 819-825 More about this Journal
Abstract
The present study was done to assess the diversity of the bacterial community associated with Ulva pertusa collected from Jeju Island using Restriction Fragment Length Polymorphism (RFLP) marker analysis. For RFLP analysis, a total of 145 bacterial strains associated with Ulva pertusa were screened and cultivated using Marine agar and R2A agar. The PCR amplicons of the 16S rRNA gene from all the isolated strains were digested with HaeIII and RsaI restriction enzymes and then classified into different groups according to their restriction patterns. Strains selected based on the RFLP patterns showed more than 91% 16S rRNA gene sequence similarity when compared with known bacterial species, which include 4 phyla - proteobacteria (alpha-proteobacteria, beta-proteobacteria, gamma-proteobacteria - 63%), firmicutes (11%), actinobacteria (4%), bacteroidetes (22%)–as well as 7 classes (actinobacteria, flavobacteriia, cytophagia, bacilli, α-proteobacteria, γ-proteobacteria, β-proteobacteria), 13 orders, 18 families, and 27 genera. These results confirmed a wide diversity of bacterial communities as contrasted with other regions. The newly isolated 10 strains, which show 16S rRNA sequence similarity of <97% compared to previously identified bacteria, could be noble species. Further experiments, such as morphological, physiological, and biochemical classification, are necessary to confirm the novelty of the newly isolated 10 strains.
Keywords
Bacterial community; phylogenetic; RFLP analysis; Ulva pertusa; 16S rRNA;
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