• Journal of Plant Biotechnology
• /
• v.35 no.1
• /
• pp.47-53
• /
• 2008

Effects of growth regulators and culture conditions on seed germination, haustorium development, and callus formation of Korean mistletoe (Viscum album var. coloratum (Kom.) Ohwi) were described. Histological examination showed that seed of V. album contained one or two zygotic embryos with rod shape, and actively dividing cells were mainly distributed in radicle region rather than cotyledon of zygotic embryo. The most significant factor for seed germination and haustorium development of V. album was the requirement of the light. Various growth regulators examined in this study failed to substitute the effect of the light on seed germination. The frequency of callus formation was highest at 27.3% when flower buds were cultured onto B5 medium containing $0.1\;mgl^{-1}$ IAA. Explants from other organs were recalcitrant in forming calluses. Culture conditions described in this study could be applied for production of useful metabolites and multiplication of V. album in future.

• Korean Journal of Plant Resources
• /
• v.21 no.4
• /
• pp.341-345
• /
• 2008

Two orchid species of Calanthe discolor Lindl. and C. discolor for. sieboldii (Decne.) Ohwi, which have different form flower color and size. They were crossed in mid April by artificial pollination, and the F1 hybrid seeds were collected mid October. Germination of seeds was investigated on pre-treatment of seeds and under the various environmental conditions. Germination was promoted by moisture absorption and ultrasonic treatment of seeds. Dark culture of F1 hybrid seeds enhanced germination and protocorm formation, and development into seedlings compared with light culture. Although, plant growth regulators such as NAA and BA had a slightly promotive effect on seed germination and protocorm growth, regenerated seeding were showed abnormal growth patterns. Regenerated F1 hybrid plantlets were successfully transferred to pot.

• Journal of Korean Society of Forest Science
• /
• v.100 no.2
• /
• pp.131-135
• /
• 2011

To examine the sex morph, fruiting characteristics and seed viabilities of Acer palmatum var. matsumurae (Koidz.) Makino, the flowers and seed-sets of 101 trees were collected from early April to late October 2010, and floral structure and seed viability were studied. Among investigated 101 trees, 73.3% of trees were protandrous (PA: pollen is shed before stigmas are receptive) and 26.7% were protogynous (PG: stigmas are receptive before pollen is shed). Acer palmatum var. matsumurae has heterodichogamous sexual system. Number of flowers and seed-sets per inflorescence are significantly different between sex morphs, and PA has more flowers and seed-sets per inflorescence than PG. Rates of sound seeds and decayed seeds are significantly different between sex morphs. Rates of sound seeds were 46.4% in PA, but 65.6% in PG. and rates of decayed seeds were 49.6% in PA, but 23.6% in PG. The differences of decayed seeds' rates between sex morphs might be related with the timing of pistil flower blooming and insect pests' outbreak.

• Journal of Bio-Environment Control
• /
• v.22 no.1
• /
• pp.61-64
• /
• 2013

This study was conducted to investigate the general characteristics of raw okra seeds and the functional components of roasted okra seeds. The number of okra seed per pod was 78 in 'Greensod' and 88 in 'Beny'. The weight of okra seed per pod of 'Greensod' and 'Beny' were 4.4 g and 6.3 g, respectively. Free amino acid contents of the stir-fry and fresh okra seeds were measured as $2.69mg{\cdot}g^{-1}$ and $0.31mg{\cdot}g^{-1}$. Total polyphenolic compound content of the stirfry okra seeds was estimated as $12.61mg\;CGA{\cdot}g^{-1}$, compared to $2.54mg\;CGA{\cdot}g^{-1}$ fresh okra seeds, Thus, free amino acid and total polyphenolic compound contents in the stir-fry okra seeds were higher than fresh one, Antioxidant activities, such as DPPH and ABTS radical scavenging in the stir-fry okra seeds was the higher than fresh okra seeds.

• Korean Journal of Medicinal Crop Science
• /
• v.7 no.3
• /
• pp.213-217
• /
• 1999

Seed coating and pelleting techniques have been used in many crops to improve the germination vigor of tiny and light seeds. Cultivated Chinese-foxglove (Rehmannia glutlnosa) has been infected by various types of virus derived from vagetative propagation of rootstock. Seed characteristics and alginate-coated seed germination rate have been investigated to get basic information for healthy seedling production through seed propagation. Chinese-foxglove showed different numbers of seeds per pod from 61 in Seocheon local to 207 in Jiwhang 1 and 1,000grain weight also varied from 70mg of Seocheon local to 130mg of Jiwhang 1. Seeds of Chinese-foxglove has dormancy because that seed collected last year more stimulated than that of this year at germination test. Optimum alginate concentration for pellected seeds germination was 2%.

• Korean Journal of Plant Resources
• /
• v.17 no.2
• /
• pp.75-81
• /
• 2004

This study was conducted to investigate effects of cryopreservation by vitrification on the seed germination rate and growth and physiological properties of seedlings of Maackia amurensis. Cryopreservation significantly decreased the germination rate of seeds of M. amurensis, but the reduction of germination rate was mitigated by the treatment of cryoprotectant (plant vitrification solution, PVS2) before plugging into liquid nitrogen and fast thawing rate after cryopreservation. Long-term PVS2 exposure decreased seed germination rate, whereas cryopreservation time didn't have influence on seed germination rate. In addition, growth and physiological properties of seedlings were not affected by PVS2 exposing time and cryopreservation time. Therefore cryopreservation could be widely used as a technique of long-term ex situ conservation without any damage and deterioration of cells or tissues of the forest seeds. However, in order to increase the effect of cryopreservation, we have to develope the lower toxic cryoprotectant and suitable techniques to the structural or chemical properties of a variety of seeds.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.31 no.1
• /
• pp.19-23
• /
• 1986

To get the basic information about ginseng seed production, yield of seed and seed quality in 27 fields were investigated. Yield of seed ranged from 4 to 10 litres per 100 kan (180cm x 90cm). Ratio of under 4mm seed is 12%, 66% for 4 to 5mm and 22% for over 5mm. Seed weight and ratio of over 4mm seed showed the decreasing tendency with the increase of seed yield per unit area. The seed yield harvested from the selected mother plants was lower than that from all plants, but seed weight and ratio of over 4mm seed were high in fields using the selected mother plants. It showed a positive correlation between length and width of seed, but no correlation between thickness and length or width. Optimum yield for high quality seed appeared under the 7 to 8 litres per 100 kan.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2020.08a
• /
• pp.68-68
• /
• 2020

최근 농촌진흥청과 기상청에서 발표한 연구자료에 따르면 2020년 기준으로 국내 경지면적의 10% 가량이 이미 아열대 기후대에 속하고 있을 뿐 현재 추세대로 온실가스가 배출될 경우 2100년경 우리나라의 대부분 지역은 아열대 기후대에 속하게 될 것으로 전망되고 있다. 이에 국가 기관뿐만 아니라 많은 지자체에서 기후변화에 대응하여 아열대 작물을 도입하기 위한 시도를 활발하게 추진하고 있다. 아열대 작물 중 국내에서 3번째로 많이 활용되고 있는 삼채는 기능성 물질을 다량으로 함유하고 있어 수요도가 지속적으로 증가하고 있다. 하지만, 삼채의 경우 일반적인 원예 작물과는 달리 실생 종자를 상업적으로 이용하고 있어 종자들의 발아율이 균일하지 못할 뿐만 아니라 발아율이 낮은 문제가 있는 것으로 보고되고 있다. 이에 삼채의 경우 농가에서 정식 시기를 맞추기 어려울 뿐만 아니라 대규모 생산을 위한 공정육묘 시스템의 적용이 어려운 상황에 있다. 종자 프라이밍 기술은 잠재적으로 종자 발아와 식물 생장을 빠르고 균일하게 해줄 수 있는 방법으로 알려져 있지만, 현재 삼채에서는 프라이밍 처리가 종자 발아에 미치는 영향에 대해서 검토한 사례가 없다. 따라서 본 연구는 삼채의 파종 효율을 증진시키기 위한 목적으로 몇 가지 프라이밍 처리 방법이 삼채의 발아특성에 미치는 영향을 검정하고자 하였다. 삼채 종자는 (주)아람종묘사의 종자를 구매하여 강릉원주대학교 생명과학대학에서 수행하였다. 프라이밍 처리는 Hydropriming은 증류수, Osmopriming은 CaCl2 1.0%, Halopriming은 Polyrthylene glycol 6000(PEG) 13.5%의 3가지를 사용하였으며, 각 프라이밍 처리 시간은 6시간, 12시간, 18시간, 24시간, 48시간 총 5가지 방법으로 처리되어 대조구를 포함 총 16개의 처리를 하였다. 프라이밍 처리 된 종자는 페트리디쉬 (50mm)에 30립씩 치상하였다. 프라이밍 각 처리구별 4반복으로 하였으며 24시간 암(Black), 온도 20℃의 조직배양실에서 치상 후 10일간 조사하였다. 본 실험 결과 대조구는 38% 발아율을 가졌으며, 프라이밍 처리 시간에 따른 각 처리구들은 대조구에 비해 발아율이 높아짐을 확인할 수 있었다. 그 중 Hydropriming 12시간 발아율 51%, Osmopriming 18시간 발아율 68%, Halopriming 48시간 발아율 65%로 각 프라이밍 처리구별 가장 좋은 발아율을 확인할 수 있었다. 결론적으로 삼채에서 프라이밍 처리 기술의 적용은 발아율의 향상과 발아기간의 정형화를 유도할 수 있어 농가에서의 일반 육묘는 물론이고 대규모 공정육묘를 위한 저렴하고 안전한 방법 중 하나로써 이용될 수 있을 것으로 기대되어 진다.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.32 no.3
• /
• pp.330-335
• /
• 1987

This study was carried out to know the effect of seed position on the size, contents of ginsenosides, free sugars, and fatty acids in ginseng seeds. Seed positions were classified by the three portions as center, middle and border in a seed cluster. Seed weight at center was light remarkably in comparison with those of seeds of at border and middle. The weight of embryo plus endosperm was in same tendency as seed weight. Percentage of single-seeded berry was smaller than that of the double-seeded, and the triple-seeded was rare. The percentage of the single-seeded increased from the border to the center. Size of the single -seeded seed was smaller than that of the double- seeded. Rate of dehiscence did not differ among different seed positions. The major ginsenosides in seed were Re, Rb$_1$, and Rb$_2$. The contents of Rb$_2$ and total saponin were highest in border, least in center, but reversed in Re and Rd. Major free sugars in seed were sucrose and glucose. The sucrose content was gradually decreased according to the seed position from border to center. Major fatty acids in the seed were oleic and linoleic acid. Contents of palmitic and linolenic acid were different according to the seed position.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.24 no.2
• /
• pp.155-162
• /
• 2004

Different adhesives(CF-clear, arabic gum, cethylmethyl cellulose, polyvinyl alcohol, polyvinyl pyrrolidone, hydroxy propyl cellulose, methyl cellulose) and particulate matters(bentonite, kaolin, calcium carbonate, peatmoss, talcum, vermiculite, zeolite, calcium hydroxide, plaster, calcium phosphate, vermiculite + calcium carbonate, vermiculite + kaolin, vermiculite + talcum) were evaluated by coating index method. According to the weight of single coated seeds, CF-clear and polyvinyl alcohol appeared to be the best coating adhesives for red clover and tall fescue seeds. Both vermiculite and vermiculite + talc(l : 1) for red clover, and vermiculite + talc(l : 1) for tall fescue appeared to be the best particulate matter(P < 0.01). Percent friability showed excellent results for the arabic gum, CF-clear, and polyvinyl alcohol when compared to others(p < 0.01). The coating index showed high with vermiculite and vermiculite + talc(l : 1) in coating red clover seed, and talc, vermiculite, and vermiculite + talc showed good coating index in coating tall fescue seed.