• Title/Summary/Keyword: 종자저장

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Effects of Ripening Temperature on Starch Structure and Storage Protein Characteristics of Early Maturing Rice Varieties during Grain Filling (조생종 벼의 평야지 재배에 따른 등숙 온도 차이에 의한 전분구조 및 종자 저장단백질 특성 연구)

  • Kwak, Jieun;Lee, Jeom-Sig;Won, Yong-Jae;Park, Hyang-Mee;Kwak, Kang-Su;Kim, Mi-Jung;Lee, Choon-Ki;Kim, Sun-Lim;Yoon, Mi-Ra
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.63 no.2
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    • pp.77-85
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    • 2018
  • This study was performed to understand the effects of filling stage temperature on the characteristics of starch and storage protein and the quality of rice grains. Eight early maturing rice varieties were cultivated in Cheolweon (latitude $38^{\circ}15^{\prime}N$) and Suwon (latitude $37^{\circ}16^{\prime}N$) areas in Korea. Rice grown in Suwon, with relatively high ripening period temperatures, showed significantly reduced head rice ratio and eating qualities, higher protein and lower amylose contents than rice grown in Cheolweon. In rice that ripened under high temperature conditions, the starch contained significantly less short-chain amylopectin (DP < 12) but more intermediate- (DP 13-24) and long- (DP > 25) chain amlylopectin compared to rice that ripened under normal conditions. In addition, the electrophoretic pattern of rice storage protein under high- temperature conditions revealed decreased prolamin and increased glutelin contents.

Environment Factors for Germination, Growing and Storage of Sprout Vegetables of Coreopsis tinctoria Nutt., Saussurea pulchella (Fisch.) Fisch. and Matricaria recutica L. (국화과 기생초, 각시취 및 저먼캐모마일 새싹채소의 발아, 재배 및 저장에 미치는 환경요인)

  • Lee, Moo-Yeul;Shin, So-Lim;Chang, Young-Deug;Lee, Cheol-Hee
    • Korean Journal of Plant Resources
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    • v.22 no.2
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    • pp.136-144
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    • 2009
  • Germination tests on 3 species that have potential for use as sprout vegetables, such as Coreopsis tinctoria, Saussurea pulchella, and Matricaria recutica, were conducted for 20 days under different temperatures of $15{\sim}30^{\circ}C$, and conditions of light and dark. C. tinctoria showed germination of 83% under $15^{\circ}C$ and dark condition after 4 days, S. pulchella 51.7% under $25^{\circ}C$ and light condition after 20 days, and M. recutica 90.3% under $25^{\circ}C$ and dark condition after 4 days. To investigate optimum plantlet size before greening treatment, seeds germinated were allowed to grow under darkness. The optimum growth of C. tinctoria was obtained under $30^{\circ}C$ after 5 days, S. pulchella under $25^{\circ}C$ after 6 days, and M. recutica under $20^{\circ}C$ after 6 days. Greening treatment resulted in diminished longitudinal growth, but C. tinctoria and S. pulchella showed more vigorous latitudinal growth. Days required before marketing as sprout vegetables were different according to species - Three days of greening was good for C. tinctoria, 2 for S. pulchella, no greening for M. recutica. Generally, tightly sealed containers for minimum water loss were recommended for storage of sprout vegetables after harvest. However, storage methods for each species were different depending on various factors like temperature of storage, presence of ventilation holes of storage packages and forms of marketing. More detailed research for above 3 species is proposed.

Change of Seed Dormancy at Different Storage Conditions in Rice (벼 저장조건에 따른 종실의 휴면성 변화)

  • 서기호;김용욱
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.3
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    • pp.270-277
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    • 1994
  • This study was conducted to investigate change of seed dormancy during storage in rice. The result of germination rate observed at different storage duration after harvest indicated that two Japonica rice varieties, Sangpungbyeo and Hwaseongbyeo, tow sare type local varieties, Jakwangdo and Mongeunsare, and a Tongil type rice, Samgangbyeo, showed no dormancy in rice seed harvested at 40 days after heading, However, weak dormancy was found in matured rice seed of Sobaekbyeo and Odaebyeo, and strong seed dormancy was found in Jungwonbyeo, IR-20 and IR-42. Rice seed stored for 30 days after harvest at 2$0^{\circ}C$ constant temperature or 25/15$^{\circ}C$ of day /night temperature fluctuation showed higher germination rate above 90% in all varieties tested. But germination rate of seeds stored at 4$^{\circ}C$ for 30 to 180 days showed varietal difference, higher in rice varieties having weak seed dormancy and lower in strong dormancy varieties. Days to 80% germination and average days for germination were shortened by storage for 30 to 180 days after harvest at 2$0^{\circ}C$, 4$^{\circ}C$ with RH 40% or day /night 25/15$^{\circ}C$ temperature condition in all rice varieties tested, but the degree of acceleration was lower at the condition of 4$^{\circ}C$ with RH 40%.

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Effects of heat treatments on the microbial reduction and germination rates of red radish sprout seeds (Raphanus sativus) (열처리 방법이 적무 새싹종자(Raphanus sativus)의 미생물 저감화 및 발아율에 미치는 영향)

  • Jun, So-Yun;Lee, Yeon-Kyung
    • Food Science and Preservation
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    • v.21 no.4
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    • pp.544-548
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    • 2014
  • This study was conducted to investigate the effects of various heat treatments on the microbial reduction and germination of red radish seeds for the development of effective and economical sterilization methods of improving microbial safety without reducting the germination rate. Hydrothermal treatment was conducted at 60, 65, 70, 75, 80, and $90^{\circ}C$ for 30 and 60 seconds, and dry heat treatment was performed at 70, 80, 90, and $100^{\circ}C$ for 5 minutes. In the seeds that underwent the hydrothermal treatment, time had little effect on the microbial reduction. There was no significant microbial reduction over time. However, there was significant microbial reduction as temperatures increased (p<0.001). The total plate count (TPC) was reduced by more than 3 logs, and Listeria monocytogenes was not detected at temperatures above $70^{\circ}C$. In the seeds that were subjected to the dry heat treatment, the TPC and the population of the L. monocytogenes were significantly reduced as the temperatures increased (p<0.001). After treatment at $100^{\circ}C$ for 5 minutes, the TPC and the L. monocytogenes were reduced by 3 logs. As with the microbial reduction, time had little effect on the germination. There were no significant changes in the germination after the hydrothermal treatment over time; but at the temperatures above $75^{\circ}C$, the germination rate significantly decreased as the temperature increased (p<0.001). When the seeds were soaked after the hydrothermal treatment, their germination was stimulated. The dry heat treatment at temperatures of $80^{\circ}C$ and higher significantly decreased the germination rate as the temperature increased (p<0.001). Dry heat treatment before the germination of the seeds soaked in distilled water for three hours significantly decreased the germination at temperatures greater than $90^{\circ}C$ (p<0.05). This study showed that appropriate heat treatments can increase the microbiological safety and germination of red radish seeds.

Study on the Development of Measuring System for Fermentation Degree of Liquid Swine Manure Using Visible Ray (가시광선을 이용한 돈분뇨 액비 부숙도 측정장치 개발에 관한 연구)

  • Choi, D.Y.;Kwag, J.H.;Park, K.H.;Song, J.I.;Kim, J.H.;Kang, H.S.;Han, C.B.;Choi, S.W.;Lee, C.S.
    • Journal of Animal Environmental Science
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    • v.16 no.3
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    • pp.227-236
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    • 2010
  • This study was conducted to develop an measuring system and method for fermentation degree of liquid swine manure by visible ray. The constituent changes of liquid swine manure were examined. pH gradually increased with time, but EC gradually decreased. Malodor strength decreased gradually with aeration treatment with time. Control needed more time to decrease malodor strength than aeration treatment. In aeration treatment, there was no germination of seeds (radish, chinese cabbage) up to 6 weeks and germination rate at 15th week was over 50%. However, in control, there was no germination up to end of experiment. Circular chromatography method showed that there was change after 10th week in aeration treatment but there was no change up to end of experiment in control. As a result, the fermentation degree of liquid swine manure would have relations among pH, EC, germination rate, malodor concentration, and reaction of circular chromatography. The simple analytical instrument for liquid swine manure consisted of a tungsten halogen and deuterium lamp for light source, a sample holder, a quartz cell, spectrometer for spectrum analyzer, a malodour measuring device, a software, etc. Results showed that the simple analytical instrument that was developed can approximately predict the fermentation degree of liquid swine manure by visible ray. Generally, the experiment proved that the simple analytical instrument was reliable, feasible and practical for analyzing the fermentation degree of liquid swine manure.

식물성 유지의 phytosterol, tocopherol 분석 및 CLA를 함유한 재구성 지질의 합성

  • 조은진;이기택
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 2003.04a
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    • pp.103.2-104
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    • 2003
  • 식물종자 중 참깨, 호두, 통밀, 미강 등을 원료로 하여 8$0^{\circ}C$, soxhlet extractor에서 6시간 동안 추출하여 수율을 알아본 후, 추출된 유지의 지방산 조성을 분석하고, phytosterol 및 tocopherol의 함량을 비교.분석하였다. 추출결과, 호두가 56.11%로 가장 높은 수율을 보였으며, 그 뒤로 참깨, 미강, 통밀 등이 각각 35.65, 14.83, 1.56% 등을 나타내었다. GC에 의한 지방산 조성 분석 결과, 추출된 식물유지 모두 linoleic acid, oleic acid등이 각각 평균 45.19, 32.70% 등으로 가장 높은 비율을 차지하였으며, 그 뒤로 palmitic acid, linolenic acid, stearic acid 등이 각각 평균 14.97, 3.52, 2.95 ㏖% 등의 비율을 나타내었다. GC에 의한 phytosterol 분석결과, 참깨, 호두, 통밀, 미강 등의 총 phytosterol 함량은 평균 1.20%의 비율을 보였고, HPLC에 의한 tocopherol 분석결과, 총 tocopherol 함량은 평균 1.52%의 함량을 보이는 것으로 조사되었다. 한편, 추출된 식물성유지 4종과 Conjugated linoleic acid (CLA)를 각각 1:3 ㏖ 비율로 혼합한 후 고정화 효소인 IM60과 24시간 동안 반응하여 재구성지질을 합성하고 GC에 의해 지방산 조성을 분석하였다. 총 CLA의 함량이 가장 많은 것은 통밀로서 23.75㏖%를 나타내었고, 그 뒤로 미강, 호두, 참깨 등이 각각 19.15, 16.28, 13.46㏖% 등의 함량을 나타내는 것으로 조사되었다.

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손바닥 선인장 열매 추출물의 기능성

  • 김정옥;이기동;권중호
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 2003.10a
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    • pp.142.2-142
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    • 2003
  • 손바닥 선인장(Opuntia ficus indica)은 중심자목 선인장과에 속하는 다년생 초본으로 열매는 서양배 모양이며 많은 종자가 들어있고 다량의 점질물을 함유하고 있다. 손바닥 선인장 열매(prickly pear pulp)는 14.5%의 고형분 함량을 가지고 있으며, 0.21%의 단백질, 0.12%의 지방, 0.44%의 회분, 0.19%의 pectin 이외에 미량의 비타민 A와 C 그리고 여러 종류의 무기질을 포함하고 있다. 주요 당류로서 sucrose(68.7%), fructose(18.0%) 및 glucose(12.8%)를 함유하고 있으며, 점질다당류의 구성분인 mannose 가 0.5% 포함되어 있다. 손바닥 선인장 열매는 높은 혈중 콜레스테롤, 염증 및 비만 등의 치료에 유용한 것으로 알려져 있다. 본 연구에서는 손바닥 선인장 열매 추출물의 기능적 특성을 반응표면분석에 의해 모니터링 하였다. 그 결과 환원당 함량은 최대값이 33.39 mg%로 추출온도 69.87$^{\circ}C$, 추출시간 68.84 min및 시료에 대한 용매비 16.29 ml/g일 때였다. 총 플라보노이드 함량은 추출온도 89.7$0^{\circ}C$, 추출시간 170 min 및 시료에 대한 용매비 17.07 ml/g일 때 2,03 mg%로 최대값을 나타내었다. 펙틴 함량은 추출온도 96.54$^{\circ}C$, 추출시간 135.28 min및 시료에 대한 용매비 20.02 nWg일 때 최대값 82.04 mg%를 나타내었으며, 비타민 C 함량은 추출온도 61.8$0^{\circ}C$, 추출시간 101.98 min 및 시료에 대한 용매비 12.14 ml/g일 때 최대값이 3.76 mg%였다.

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Prevention from Microbial Post-harvest Injury of Fruits and Vegetables by Using Grapefruit Seed Extract, a Natural Antimicrobial Agent -Isolation of antimicrobial substance from grapefruit seed extract- (천연항균제처리에 의한 과채류의 선도유지 및 병해방지에 관한 연구 -Grapefruit 종자추출물로 부터 활성물질의 분리를 중심으로-)

  • Jo, Seong-Hwan;Kim, Gi-Ok;Lee, Geun-Hoe
    • Food Science and Preservation
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    • v.1 no.1
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    • pp.1-7
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    • 1994
  • To investigate the effect of several bactericides and fungicides against fruit & vegetable decay grapefruit seed extract(GFSE) was tested in a dilution solution. GFSE was shown to be effective against decay and rind breakdown and to extend the shelf-life of fruits and vegetables during storage and tiansport. Antimicrobial efficiency of GFSE on a wide spectra of gram + & - bacteria, moulds and yeasts was demonstrated by the measurement of minimal inhibitory concentrations performed (Bacteria 10-500ppm, Fungi:250-1,000ppm, Yeasts:100-250ppm). GFSE was separated and extracted into water-soluble fraction, water-insoluble and non-dialyzed fraction to isolate the antimicrobial substances. The water-soluble fraction showed the most active antimicrobial effect. The antimicrobial substances were isolated by gas chromatography. As the result of the isolation using GC, Peak-D was found to be the antimicrobial compound in GFSE. The identification of the most antimicrobial substance was carried out by using GC-MS.

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Seed Germination and Seedling Emergence of Indian jointvetch (Aeschynomene indica L.) in Different Conditions (자귀풀 종자의 발아 및 출아에 미치는 환경요인)

  • Jin, Chang Hao;Uddin, Md. Romij;Pyon, Jong-Yeong
    • Korean Journal of Weed Science
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    • v.30 no.1
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    • pp.25-33
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    • 2010
  • Several experiments were conducted in growth chambers and a greenhouse to determine the influence of various environmental factors on seed germination and seedling emergence of Indian jointvetch. Fully matured seeds of Indian jointvetch germinated only 42%. The germination percent increased as the storage temperature increased with time. More than 90% seeds germinated when the seeds were kept at $40^{\circ}C$ for seven months, but germination was 58.9 and 55.1% when kept at 25 and $4^{\circ}C$, respectively. Non-dormant seeds of Indian jointvetch germinated 91.1 and 92.4% at 30 and $30/25^{\circ}C$, respectively. Germination percent increased with increasing both prethermal temperature and time. The prethermal temperature of $90^{\circ}C$ for 40 minutes was the best for maximum germination (94.5%). Germination and growth of Indian jointvetch tended to decrease slightly until -0.3 MPa osmotic potential (water stress induction) and then declined drastically and the seeds did not germinate at below -0.5 MPa osmotic potential. Indian jointvetch seems to grow well in moist and flooding conditions since emergence and growth of seedling increased with increasing soil moisture content and the water level.

Variation of Fractionated Protein Content by Solubility in Korean Local Sorghum Seed (국내 수수 종자의 용해도별 단백질 함량 변이)

  • Park, Sei Joon;Park, Jun Young;Hwang, Su Min;Seo, Myung Chul;Kim, Tae Wan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.3
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    • pp.245-251
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    • 2014
  • Sorghum seed is traditionally used as health supplements and the secondary food mixed with rice in Korea. While the research of reserve protein in sorghum seed have been carried out in many countries used as major food, much less is known about reserve proteins of Korean local sorghum seeds. To obtain protein characteristics in 20 Korean local sorghum seed, quantitative content of reserve protein was determined after fractionation by modified 'Osbone' method and ${\alpha}$-kafirin of prolamin was determined by SDS-PAGE. Mean albumin, globulin, prolamin and glutelin contents based on total seed protein content of 20 Korean local sorghum seed were 6.2%, 0.9%, 57.9% and 35.1%, respectively. Sorghum cultivar with high prolamin were 'Whin-susu', 'Whin-Chalsusu', 'Whanggeum-Chalsusu', and 'Daepungshushu'. Sorghum cultivar with high ${\alpha}$-kafirin were 'Whin-susu', 'Geumsan-Chalsusu', 'Whin-Chalsusu', and 'Jangmok-susu'. Among the 20 varieties, 'Whin-susu' and 'Whin-Chalsusu' were selected as high ${\alpha}$-kafirin and prolamin sorghum cultivar, which showed 64.5 and 71.9% of prolamin contents, respectively.