KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Variation of Fractionated Protein Content by Solubility in Korean Local Sorghum Seed

국내 수수 종자의 용해도별 단백질 함량 변이

  • Park, Sei Joon (Institute of Ecological Phytochemistry, Hankyong National University) ;
  • Park, Jun Young (Institute of Ecological Phytochemistry, Hankyong National University) ;
  • Hwang, Su Min (Department of Plant Life and Environmental Science, Hankyong National University) ;
  • Seo, Myung Chul (Crop Environment research division, NICS, RDA) ;
  • Kim, Tae Wan (Crop Environment research division, NICS, RDA)
  • 박세준 (국립한경대학교 식물생태화학연구소) ;
  • 박준영 (국립한경대학교 식물생태화학연구소) ;
  • 황수민 (국립한경대학교 식물생명환경과학과) ;
  • 서명철 (농촌진흥청 국립식량과학원 작물환경과) ;
  • 김태완 (농촌진흥청 국립식량과학원 작물환경과)
  • Received : 2013.12.17
  • Accepted : 2014.02.25
  • Published : 2014.09.30
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Abstract

Sorghum seed is traditionally used as health supplements and the secondary food mixed with rice in Korea. While the research of reserve protein in sorghum seed have been carried out in many countries used as major food, much less is known about reserve proteins of Korean local sorghum seeds. To obtain protein characteristics in 20 Korean local sorghum seed, quantitative content of reserve protein was determined after fractionation by modified 'Osbone' method and ${\alpha}$-kafirin of prolamin was determined by SDS-PAGE. Mean albumin, globulin, prolamin and glutelin contents based on total seed protein content of 20 Korean local sorghum seed were 6.2%, 0.9%, 57.9% and 35.1%, respectively. Sorghum cultivar with high prolamin were 'Whin-susu', 'Whin-Chalsusu', 'Whanggeum-Chalsusu', and 'Daepungshushu'. Sorghum cultivar with high ${\alpha}$-kafirin were 'Whin-susu', 'Geumsan-Chalsusu', 'Whin-Chalsusu', and 'Jangmok-susu'. Among the 20 varieties, 'Whin-susu' and 'Whin-Chalsusu' were selected as high ${\alpha}$-kafirin and prolamin sorghum cultivar, which showed 64.5 and 71.9% of prolamin contents, respectively.

우리나라 수수 유전자원 20품종에 대한 종자 단백질의 정량적 및 정성적 특성을 분석하였다. 수수 종자의 수용성 단백질의 분획은 'Osborne 방법'의 변형된 방법으로서 알부민, 글로불린, 프롤라민 및 글루텔린을 순차적으로 분리하여 정량분석을 실시하였다. 수수 종자의 저장단백질인 프롤라민 단백질의 조성을 1차 전기영동으로 비교 분석하였다. 1. 총 단백질 함량 대비 분획단백질의 함량은 알부민 단백질은 6.2%, 글로불린 단백질은 0.9%, 프롤라민 단백질은 57.9% 및 글루텔린 단백질은 35.1%의 함량을 나타내었다. 2. 품종별 비교에서 프롤라민 단백질의 함량이 높은 품종은 흰수수, 흰찰수수, 황금찰수수 및 대풍수수로 결정되었다. 3. ${\alpha}$-kafirin의 함량이 높은 품종은 흰수수, 금산찰수수, 흰찰수수 및 장목수수로 나타났다. 4. 흰수수와 흰찰수수가 고 프롤라민 및 고 ${\alpha}$-kafirin 품종임을 제시하였다.

Keywords

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