• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.1
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• pp.21-27
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• 2008

Germination of soybean and growth of its sprouts should be affected by storage condition and period of seeds. The experiment was conducted to examine the effects of period stored at cold room and storage conditions on their growth and morphological characters. The seeds, cv. Pungsannamulkong, were stored at $3^{\circ}C$ for 1 or 2 years, and by the three different storage methods of indoor, cold room, or transfer of indoor to cold room. Compared to the seeds stored for 2 years, one-year seeds had higher rate in hypocotyl of longer than 7 cm, root and hypocotyl lengths, and more hypocotyl fresh weight although there was not significant difference in total fresh weight. In the storage method experiment, one-year seeds stored at cold room and by transfer of indoor to cold room showed better growth, more lateral roots and fresh weights, although only two-year seeds stored at cold room did the same response, indicating that growth of soybean was affected by period and method of seed storage.

• Journal of Ginseng Research
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• v.28 no.4
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• pp.215-218
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• 2004

This study were conducted to investigate the viability and germination of dehisced Panax ginseng seeds stored for long period in the storage chamber with $5^{\circ}C\;and\;30{\%}$ humudity. The staining reaction times for viability test were 150 min, 90 in, 60 min at $0.1{\%},\;0.5{\%}\;and\;1{\%}$ triphenyltetrazolim chloride(TTC), respectively. the more the storage period, the less the healthy seeds ratio. It was $96.6{\%},\;89.2{\%},\;63.4{\%}$ for 1-year storage, 7-years storage, 9-years storage, respectively. Germination ratio were $84.0{\%},\;80.5{\%},\;73.5{\%},\;2.5{\%}$ for 1-year stroage, 6-years storage, 7-years storage and 9-years storage, respectively. Therefore it was confirmed that ginseng seed can be stored up to 6-7 years.

• Korean Journal of Agricultural and Forest Meteorology
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• v.6 no.3
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• pp.183-189
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• 2004

This study was conducted to examine the changes in the seed physical characters and germinative properties of three tree species at three different storage times in order to determine the most suitable storage time and condition. Seed physical characters and germinative properties were examined from seeds of Fraxinus rhynchophylla, Zanthoxylum schinifolium and Staphylea bumalda that were stored at -18C for 1, 3 and 10 years, and the growth performance and physiological characters of their seedlings were analyzed. Seed physical characters and germinative properties showed significant differences between three storage times as well as three tree species. Seed moisture content of F rhynchophylla and S. bumalda and seed fresh weight of Z. schinifolium decreased with increase of storage time. Storage time represented negative correlation with moisture content and fresh weight (r = -0.822, P<0.01). Seed percent germination of Z. schinifolium and S. bumalda stored for 3 and 10 years decreased less than 15% and 10.7% respectively. Mean germination times for seed of F. rhynchophylla, Z. schinifolium and S. bumalda were delayed 11.9, 5.7 and 9.7 days after a storage time of 10 years, respectively. However the growth and viability of seedlings didn't show a special pattern for storage time and tree species.

• Korean Journal of Plant Resources
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• v.17 no.2
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• pp.75-81
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• 2004

This study was conducted to investigate effects of cryopreservation by vitrification on the seed germination rate and growth and physiological properties of seedlings of Maackia amurensis. Cryopreservation significantly decreased the germination rate of seeds of M. amurensis, but the reduction of germination rate was mitigated by the treatment of cryoprotectant (plant vitrification solution, PVS2) before plugging into liquid nitrogen and fast thawing rate after cryopreservation. Long-term PVS2 exposure decreased seed germination rate, whereas cryopreservation time didn't have influence on seed germination rate. In addition, growth and physiological properties of seedlings were not affected by PVS2 exposing time and cryopreservation time. Therefore cryopreservation could be widely used as a technique of long-term ex situ conservation without any damage and deterioration of cells or tissues of the forest seeds. However, in order to increase the effect of cryopreservation, we have to develope the lower toxic cryoprotectant and suitable techniques to the structural or chemical properties of a variety of seeds.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.20-20
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• 2019

전 지구적으로 학명이 보고된 식물 종은 약 36만종이다. 그 중 종자를 생산하는 관속식물은 310,422종으로 전체 식물종의 85.7%에 달한다(IUCN, 2018). 그리고 2015년 Primely Forest Organization (PFO)에서는 야생식물의 80%가 산림 내에 서식한다고 밝혔다. 식물이 인류에게 주는 다양한 이로움은 인류의 생존과 복지증진에 막대한 영향을 미친다. 2016년 State Worlds Plants (SWP)에 따르면 인류가 현재까지 이용하는 식물종은 31,128종으로 알려져 있다. 이중 약용으로 17,810종, 식량으로 5,538종을 유용하게 이용하고 있다. 하지만 현재 전 지구적 생물멸종속도는 산업혁명 이전 보다 약 1,000배정도 빠르게 진행이 되고 있다(Science, 2017). 이에 전 지구적 생물다양성보전을 위해 생물다양성 협약, 지구식물보전전략 등이 지속적으로 발효 이행되고 있다. 시드볼트는 이러한 보전전략을 이행하기 위해 적합한 시설이다. 우리나라도 노르웨이와 전 세계적으로 2곳뿐이 없는 시드볼트를 가지게 되었다. 우리의 시드볼트는 전 지구적 재난과 재앙에 대비해 야생식물종자를 안전하게 영구 저장하는 시설이고, 우리나라는 봉화군에 위치한 국립백두대간수목원 내에 200만점 저장 규모로 조성이 되어 있다. 현재 국내 외 수집 수탁종자 48,327점이 안전하게 저장이 되어 있다. 국내 23개 종자관련 기관, 단체, 개인과 카자흐스탄 등 해외 3개국 6개 기관이 야생식물종자의 영구저장을 요청하였다. 앞으로 시드볼트는 통일시대에 대비하여 지구적 야생식물종자 저장의 컨트롤타워 역할과 종자연구의 중심기관으로 발돋움 할 것이다. 이에 따른 목표로 2050년까지 백두대간 글로벌시드볼트(BGSV)는 전세계 식물종의 13%, 40만점을 저장할 것이다. 특히 종자연구부분에서는 현재 종 맞춤형 활력검정시스템개발, 분류군별 장기저장 특성분석, 장기저장 종자수명예측 등을 고도화하여 한반도 야생식물 종자정보 플랫폼 구축, 고대 매장종자 보전 모델링 연구, 지구 종자연구 브릿지 구축 등으로 개발, 발전시킬 것이다.

• Korean Journal of Plant Resources
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• v.33 no.1
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• pp.33-39
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• 2020

This study was carried out to provide basic information for mass breeding and cultivating the saplings of Camellia japonica. The germination rates did not show definite tendency according to the changes of seed weight at room temperature and 4℃ dry storage conditions. The germination rate of 4℃ wet sand storage was increased with seed weight, but showed very low germination rate for 120 days of storage. The germination rate was above 80% in 60 days, 90 days and 120 days, respectively, at 4℃ wet filter paper storage conditions. The average days taken for those germinations were about 30 days at room temperature and 4℃ dry storage conditions, but wet storage (sand, filter paper) condition took the shortly nearly 13 days for those germinations. Leaf number, leaf dry weight, stem diameter and root dry weight were higher than total average by more than 1.21 g for seed weight. Stem length was higher than the average by more than 1.01 g, plant dry weight was higher than the average by more than 0.81 g, and stem dry weight was higher than the average by more than 0.61 g. In the case of seed weight becoming heavier, seedling growth was good but T/R ratio tended to decrease. In order to increase the mass production of seedlings using Camellia japonica seeds, we need to specify the weight and size of those seeds. The 4℃ wet filter paper storage condition was evaluated as the most efficient method for the seed storage used for seeding.

• Journal of Korean Society of Forest Science
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• v.111 no.3
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• pp.418-427
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• 2022

Our aim was to evaluate the quality of pine seeds after long-term storage and to analyze the correlation between germination and physiological characteristics. Therefore, we investigated the germination and physiological characteristics of seeds stored for different periods and storage temperatures. Pine seeds lost little viability and vigor after 12 years of storage at 4℃; it is even possible to prolong the storage period at -18℃. The deterioration process operating through long-term storage was characterized by a loss of seed vigor before a loss in seed viability. Electrical conductivity of the seed leachate was significantly higher in seeds that completely lost their viability, and was also found to be significantly correlated with the germination percentage, T50, mean germination time, and germination speed. Among the minerals leaked to the leachate, K had the highest concentration, followed by Na, Ca, Cu, Mg, and Fe, while Mn and Zn were not detected. Concentrations of K, Ca, Cu, Mg, and Fe in the leachate differed significantly by treatment, and in the cases of K, Ca, Cu, Na, and Mg, we observed large differences in the leachate between the seeds that were collected in 2003 and those collected in other years.. Germination percentage was significantly negatively correlated to the concentrations of Ca, Cu, K, Mg, and Na. The coefficient of uniformity of germination was significantly negatively correlated to Cu concentration. The other germination characteristics were not significantly correlated with the mineral concentration. Therefore, the mineral concentrations of pine seed leachate were not reliable indicators of seed deterioration when the differences in quality between seed lots were small. However, electrical conductivity could be an indicator of seed viability and vigor of pine seeds under long-term storage.

• Korean Journal of Medicinal Crop Science
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• v.4 no.1
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• pp.64-67
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• 1996

This experiment was carried out to obtain the information on seed dormancy, germinability during storage in wasabia japonica Matsum. $GA_3$ 100ppm+BA 10ppm was more effective than $GA_3$100ppm alone in breaking the dormancy. Storage of seeds pretreated with growth retardants (CCC, Uniconazol) seemed to be a useful method for preventing germination during storage. The stratification was an effective storage method to germinate the seeds for sowing them in fall.

• Journal of Korean Society of Forest Science
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• v.112 no.4
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• pp.442-450
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• 2023

This study sought to determine the desiccation tolerance and storage behavior of Sarcandra glabra seeds to assist in the establishment of an ex-situ conservation strategy for the species. The basic germination characteristics of S. glabra seeds were investigated. Subsequently, the seeds were dried to various desiccation levels to allow for analysis of their germination characteristics and seed integrity. In addition, the seeds were subjected to germination tests after being stored for different durations. The initial seed moisture content following the removal of the fleshy fruit was 32.8%, and the fresh seeds maintained their seed viability even after a decrease in the seed moisture content to 4.8%, while the germination percentage was 81.0%. The germination percentage of the seeds decreased to 55.0% and 30.0% when they were desiccated to moisture contents of 2.3% and 2.2%, respectively. By contrast, when the stored seeds were desiccated, they maintained their seed viability and vigor at a moisture content of 18.6%; however, when the moisture content decreased below 5.0%, both the seed viability and vigor significantly declined and the germination percentage fell below 20.0%. Furthermore, analysis of the seed leachate revealed that the decrease in the seed viability and vigor was associated with both increased electrical conductivity and increased concentrations of inorganic compounds such as potassium and calcium. In conclusion, the findings of this study show S. glabra seeds to possess desiccation tolerance that falls somewhere between the recalcitrant and intermediate seed types, with the observed variations depending on the degree of deterioration.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.19 no.2
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• pp.164-168
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• 2014

To determine the optimal storage method and longest possible storage period of Zostera marina seeds, we examined post-storage germination rates using different storage methods and periods for Z. marina seeds harvested in southern coast of Korea. Storage methods included in seawater at room temperature with air supply, seawater at $4^{\circ}C$, seawater at $30^{\circ}C$, an aquarium with continuous seawater circulation, seawater at room temperature in an airtight tank, a refrigerator at $-20^{\circ}C$, and a desiccator at room temperature. Germination rates of Z. marina seeds stored in seawater at $4^{\circ}C$ showed highest germination (52.0%), whereas those of seeds stored in seawater at room temperature and an aquarium were 27.4% and 17.7%, respectively. But the seeds stored in seawater at $30^{\circ}C$, a refrigerator, and a desiccator did not germinate. Storage periods were 10, 20, 30, 60, 180 and 240 days of storage. Z. marina seeds maintained germination rates of 46.4~52.4% until 10~60 days of storage, but showed rapidly decreasing germination rates after then and no germination after 240 days.