Changes in the Seed Characters and Germination Properties of Three Tree Species at Different Storage Time

저장기간에 따른 3개 수종의 종자 및 발아 특성 변화

  • 한심희 (국립과학원 산림유전자원부) ;
  • 김찬수 (국립과학원 산림유전자원부) ;
  • 장석성 (국립과학원 산림유전자원부) ;
  • 이현주 (국립과학원 산림유전자원부) ;
  • 탁우식 (국립과학원 산림유전자원부)
  • Published : 2004.09.01

Abstract

This study was conducted to examine the changes in the seed physical characters and germinative properties of three tree species at three different storage times in order to determine the most suitable storage time and condition. Seed physical characters and germinative properties were examined from seeds of Fraxinus rhynchophylla, Zanthoxylum schinifolium and Staphylea bumalda that were stored at -18C for 1, 3 and 10 years, and the growth performance and physiological characters of their seedlings were analyzed. Seed physical characters and germinative properties showed significant differences between three storage times as well as three tree species. Seed moisture content of F rhynchophylla and S. bumalda and seed fresh weight of Z. schinifolium decreased with increase of storage time. Storage time represented negative correlation with moisture content and fresh weight (r = -0.822, P<0.01). Seed percent germination of Z. schinifolium and S. bumalda stored for 3 and 10 years decreased less than 15% and 10.7% respectively. Mean germination times for seed of F. rhynchophylla, Z. schinifolium and S. bumalda were delayed 11.9, 5.7 and 9.7 days after a storage time of 10 years, respectively. However the growth and viability of seedlings didn't show a special pattern for storage time and tree species.

저장 기간이 서로 다른 종자들을 대상으로 저장 기간에 따른 종자 특성 및 발아특성 변화를 조사하고, 적정 저장 기간 및 저장 조건을 찾고자 실시하였다. 물푸레나무, 산초나무 및 고추나무의 종자는 -18$^{\circ}C$에서 1년, 3년, 10년간 저장된 것을 이용하였으며, 수종별 종자 및 발아 특성과 유묘의 생장 및 생리적 특성을 조사하였다. 3개 수종의 종자 및 발아 특성은 저장 기간 및 수종별 차이를 뚜렷하게 보여주었다. 물푸레나무와 고추나무 종자의 수분함량은 저장기간이 길어지면서 감소하는 경향을 나타냈다. 또한 종자의 무게도 저장기간이 길어지면서 감소하였는데, 특히 산초나무의 10년간 저장 종자의 생중량 감소가 뚜렷하였으며, 충실율도 10년간 저장 후 크게 낮아졌다. 저장기간은 물푸레나무 및 고추나무 종자의 수분함량 또는 고추나무 종자의 생중량과 부의 상관(r=-0.822, p<0.01)을 나타냈다. 3년 이상 저장된 산초나무 종자의 발아율은 15% 이하로 감소하였으며, 3년간 저장된 고추나무 종자의 발아율은 10.1% 이하로 급격히 하락하였다. 또한 평균발아소요일수는 종자 저장기간이 길어지면서 증가하였는데, 10년간 저장된 물푸레나무, 산초나무 및 고추나무 종자의 평균발아소요일수는 11.9일, 5.7일, 9.7일씩 각각 증가하였다. 물푸레나무와 고추나무 종자의 평균발아소요일수는 수분함량과 부의상관을 보여주었으며, 저장기간과는 정의 상관을 보였다. 그러나 종자의 저장기간에 따른 유묘의 생장 및 활력 차이는 일정한 경향을 나타내지 않았다.

Keywords

References

  1. Beadle, C. L., 1993: Growth analysis. Photosynthesis and Production in a Changing Environment, A Filed and Laboratory Manual., D. O. Hall, J. M. O. Scurlock. H. R. Bolhàr-Nordenkampf, R. C. Leegood and S. P. Long (Eds.), Chapman & Hall, London, 36-46
  2. Bolhàr-Nordenkampf, H. R. and G. Öquist, 1993: Chlorophyll fluorescence as a tool in photosynthesis research. Photosynthesis and Production in a Changing Environment, A Field and Laboratory Manual, D. O. Hall, J. M. O. Scurlock. H. R. Bolhàr-Nordenkampf, R. C. Leegood and S. P. Long (Eds.), Chapman & Hall, London, 193-206
  3. Dürr, C., and J. Boiffin, 1995: Sugarbeet (Beta vulgaris L.) seedling growth from germination ti first leaf stage. The Journal of Agricultural Science 124, 427-435
  4. Forey, P. L., C. J. Humphries, and R. I. Vane-Wright, 1994: Systematic and Conservation Evaluation. Oxford University Press. Oxford
  5. Graudal, L., E. D. Kiaer, and S. Canger, 1995: A systematic approach to conservation of genetic resources of trees and shrubs in Denmark. Forest Ecology and Management 73, 117-134
  6. Guarino, L., R. Rao, and V. Reid, 1995: Collecting Plant Genetic Diversity: Technical guidelines. CAB International, Wallingford
  7. Justice, O. L., and L. N. Bass, 1978: Principles and Practices of Seed Storage. Agriculture Handbook No. 506, Washington D.C. 289pp
  8. Khan, M. L., P. Bhuyan, U. Shankar, and N. P. Todaria, 1999: Seed germination and seedling fitness in Mesua ferrea L. in relation to fruit size and seed number per fruit. Acta Oecologia 20, 599-606
  9. Knypl, J. S., and A. A. Khan, 1981: Osmoconditioning of soybean seeds to improve performance at suboptimal temperatures. Agronomy Journal 73, 112-116
  10. Marshall, D. L., 1987: Effects of seed size on seedling success in three species of Sesbania (Fabaceae). American Journal of Botany 73, 457-464
  11. Maxted, N., B. V. Ford-Lloyd, and J. G. Hawkes, 1997: Complementary conservation strategies. Plant Genetic Conservation. N. Maxed, B. V. Ford-Lloyd, and J. G. Hawkes. (Eds.), Chapman and Hall, London, 15-39
  12. Murali, K. S., 1997: Pattern of seed size, germination and seed viability of tropical tree species in southern India. Biotropica 29, 271-279
  13. Navarro, L. and J. Guitian, 2003: Seed germination and seedling survival of two threatened endemic species of the northwest Iberian peninsula. Biological Conservation 109, 313-320
  14. Naylor, R. E. L., 1993: The effect of parent plant nutrition on seed size, viability and vigor, and on germination of wheat and triticale at different temperatures. Annals of Applied Research 28, 104-110
  15. Pill, W. G., J J. Frett, and D. C. Morneau, 1991: Germination and seedling emergence of primed tomato and asparagus seeds under adverse conditions. Hortscience 26, 1160-1162
  16. Scott, S. J., R. A. Jones, and W. A. Williams, 1984: Review of data analysis methods for seed germination. Crop Science 24, 1192-1199
  17. amet, V., J. Boffin, C. Durr, and N. Souty, 1996: Emergence and early growth of an epigeal seedling(Daucus carota L.) : influence of soil temperature, sowing depth, soil crusting and seed weight. Soil & Tillage Research 40, 25-38
  18. van Slageren, M. W., 2003: The millenium seed bank: building partnerships in arid regions for the conservation of wild species. Journal of Arid Environments 54, 195- 201
  19. Zoppo, M. D., L. Galleschi, and F. Saviozzi, 1998: Longterm storage of Araucaria bidwillii Hook. seeds. Seed Science and Technology 22, 267-270