• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.1
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• pp.537-545
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• 2018

In this study, transcriptome analysis was conducted to collect various information from Fagopyrum esculentum and Fagopyrum tataricum during the early germination stage. Total RNA was extracted from the seeds and at 12, 24, and 36 hrs after germination of Jeju native Fagopyrum esculentum and Fagopyrum tataricum and sequenced using the Illumina Hiseq 2000 platform. Raw data analysis was conducted using the Dynamic Trim and Lengths ORT programs in the SolexaQA package, and assembly and annotation were performed. Based on RNA-seq raw data, we obtained 16.5 Gb and 16.2 Gb of transcriptome data corresponding to about 84.2% and 81.5% of raw data, respectively. De novo assembly and annotation revealed 43,494 representative transcripts corresponding to 47.5Mb. Among them, 23,165 sequences were shown to have similar sequences with annotation DB. Moreover, Gene Ontology (GO) analysis of buckwheat representative transcripts confirmed that the gene is involved in metabolic processes (49.49%) of biological processes, as well as cell function (46.12%) in metabolic process, and catalytic activity (80.43%) in molecular function In the case of gibberellin receptor GID1C, which is related to germination of seeds, the expression levels increased with time after germination in both F. esculentum and F. tataricum. The expression levels of gibberellin 20-oxidase 1 were increased within 12 hrs of gemination in F. esculentum but continuously until 36 hrs in F. tataricum. This buckwheat transcriptome profile analysis of the early germination stage will help to identify the mechanism causing functional and morphological differences between species.

• Korean Journal of Plant Taxonomy
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• v.36 no.3
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• pp.163-187
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• 2006

Taxonomic studies were conducted to evaluate the interspecific relationships in Viola albida var. albida and its related taxa using seven populations for morphology, palynology and anatomy. Molecular phylogenetic studies were also examined in 28 populations including 19 Korean, four Chinese, two Japanese, one American population and two outgroups using nrITS, and 27 populations except V pinnata for trnL-F region of chloroplast DNA. Morphological differences was observed among seven populations of three species in leaf shape, but characters such as serrate number of leaf margins, petal size, pistil shape were showed overlap between populations. Pollen shape of seven populations was monad and grain shape on the polar axis was semi-angular. Morphology of aperture was tri-colporate, and the surface sculpturing was scabrate in rugulate. The grain shape of equatorial view of five populations was prolate whereas V albida var. taknhashii type 1 and V albida var. chaerophylloides type 3 were subprolate. The anatomical characters of rnidvein of leaf, petiole, peduncle, root were also described for the species. The stomatal apparatus of the leaves was observed only in abaxial surface, and the number of stoma per unit ($mm^2$) were abundant in incised than lobate or cleft margin of the leaves. The nrITS analysis shows that V. pinnata and V. dissecta was monophyletic and occupied a basal position in the V. albida var. albida and its related taxa. The other clade including infraspecific populations of V. albida, and V. eizanensis was paraphyletic. The trnL-F noncoding region analysis was similar to the ITS tree. According to the above observations in morphology, palynology, anatomy, and molecular phylogenetic analysis, the significant differences were not found except for leaf shape in Viola albida var. albida and its related taxa, therefore V. albida var. takanhashii and V. albida var. chaerophylloides were considered to be an infraspecific taxa of V. albida var. albida rather than an independent species, subvariety or variety of V. pinnata and V. dissecta.

• Journal of the Korean Society of Groundwater Environment
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• v.1 no.1
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• pp.23-32
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• 1994

To determine the apparent equilibrium constants, K$_{ad,app}$, for the adsorption reactions of trace metals on amorphous iron oxyhydroxide (AIO) in the Taebag coal mine area, time-adsorption and pH-adsorption experiments were performed for a selected bottom sediment mainly comprised of AIO from the study area. The results from the adsorption experiments indicate that most of the trace metals, except Pb, achieve equilibrium states with AIO and thus, the calculated K$_{ad,app}$ may represent the true apparent equilibrium constants. K$_{ad,app}$ and the stoichiometric coefficients of proton, x, of the adsorption reactions between the trace metals and AIO were respectively calculated from the intercepts and slopes of the regression lines of log($\Gamma$/ ［M］$_{aq}$)against pH provided by pH-adsorption experiments. The calculated K$_{ad,app}$ this study has the values of the range from 10$^{－4.5}$ to 10$^{2.75}$ , which is much different from the reported values by other investigators for simple experimental systems. K$_{ad,app}$ of this study is more or less close but not exactly pertinent to the estimated values for the other natural systems. It indicates that K$_{ad,app}$ for the adsorption reactions in the aquatic system in the study area is unique and thus should be determined befor the adsorption modelling. The calculated x of this study has the values of the range from -0.3 to 0.7, which is also much different from what most geochemists generally accept. The discrepancy in x may be due to the competition among different kinds of ionic species on the adsorption site or simulataneous occurrence of different kinds of adsorption reactions. The results from this study should help construct an appropriate adsorption model for the aquatic systems polluted by the coal mine drainage in the Taebag area. With the constructed model, one can describe the concentration variations of trace metals due to the adsorption in the system, which is an essential part of the investigation on the water quality affected by coal mine drainage in the Taebag coal field.

• Korean Chemical Engineering Research
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• v.50 no.6
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• pp.1034-1042
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• 2012

This study was performed by using an LFR (laminar flow reactor), which can be used to carry out different types of research on coal. In this study, an LFR was used to analyze coal flames, tar and soot yields, and structures of chars for two coals depending on their volatile content. The results show that the volatile content and oxygen concentration have a significant effect on the length and width of the soot cloud and that the length and width of the cloud under combustion conditions are less than those under a pyrolysis atmosphere. At sampling heights until 50 mm, the tar and soot yields of Berau (sub-bituminous) coal, which contains a large amount of volatile matter, are less than those of Glencore A.P. (bituminous) coal because tar is oxidized by the intrinsic oxygen component of coal and by radicals such as OH-. On the other hand, at sampling heights above 50 mm, the tar and soot yields of Berau coal are higher than those of Glencore A.P. coal by reacted residual volatile matter, tar and light gas in char and flame. With above results, it is confirmed that the volatile matter content and the intrinsic oxygen component in a coal are significant parameters for length and width of the soot cloud and yields of the soot. In addition, the B.E.T. results and the images of samples (SEM) obtained from the particle separation system of the sampling probe support the above results pertaining to the yields; the results also confirm the pore development on the char surface caused by devolatilization.

• Journal of Korean Society of Forest Science
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• v.80 no.4
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• pp.393-407
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• 1991

The vegetation structure within Chamaecyparis obtusa plantation was analyzed for the purpose of applying the effective forestation method for Chmaecyparis obtusa plantation, tending and regeneration in the southern districts of korea. The results were as follows ; 1. The importance percentage was high in the order of Eurya japonica, Rhus verniciflua, Chamaecyparis obtusa, Lindera erythrocarpa, Carpinus laxiflora, Styrax japonica, Viburnum dilatatum, Zanthoxylum piperitum and Smilax china among the vegetation of Chamaecyparis obtusa. Importance percentage of natural seedling of Chamaecyparis obtusa was high in lower story but gradually decreased in middle story. 2. The basal area of upper trees had a negative correlation with the density of natural seedlings in the middle and lower story, and it represents that the basal area of upper trees had some effect on the density of natural seedlings within understories. 3. The rate of the A and B class by Raunkiaer's frequency was higher in the vegetation of middle story than that of lower story. 4. By Morisita's index, the species of Chamaecyparis obtusa, Rhus verniciflua, Lindera erythrocarpa, Smilax china. Callicarpa japonica and Lindera obtusiloba were randomly distributed at lower story, but they were aggregatively distributed at middle story. At all of middle and lower story, Eurya japonica and Viburum dilatatum were randomly distributed, and Carpinus laxiflora, Zanthoxylum piperitum and Picrasma quassioides were aggregatively distributed. 5. The number of appearance species and the value of species diversity in western survey area were more than that of eastern survey area. 6. The value of species diversity at lower story was higher than that of middle story, and it represents that the number of individuals of appearance species was composed more even at lover story than middle story. 7. According to cluster analysis by similarity index, the survey areas were separated from inland and seacoast districts. 8. Judging from each stories ordination analysis by dissimilarity index, the vegetation was separated from lower and middle story, and the vegetation of lower story was more progressed succession stage than that of middle story. 9. In Chamaecyparis obtusa stands, Eurya japonica had a positive correlation with Sorbus alnifolia, Hex macropoda. Ficus erecta and Trachelospermum asiaticum, but it had a negative correlation with Zanthoxylum piperitum, Carpinus laxiflora and Parthenocissus tricuspidata. 10. In estimation of the productivity of Chamaecyparis obtusa stands, the value of SC (Conic surface) and VP (Parabolic volume) for upper trees was 94.5% and 99.63%, respectively and SC and VP of middle story was 5.49% and 0.37%, respectively. In the species of middle story, material productivity was high in order of Eurya japonica. Lindera eryhrocarpa, Rhus verniciflua. Carpinus laxiflora and Styrax japonica.

• Korean journal of applied entomology
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• v.39 no.4
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• pp.211-226
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• 2000

Genetic divergence and phylogenetic relationships among the major Korean fireflies (Hotaria papariensis, Luciola lateralis, and Pyrocoelia rufa) were studied. A portion of mitochondrial COI (403 bp) and 165 rRNA (490~504 bp) genes were sequenced, and the GenBank-registered, homologous 165 rRNA sequences of Japanese fireflies were compared (27 species of Lampyridae, one of Lycidae, and one of Rhgophthalmidae). Greatest DNA and/or amino acid sequence divergence was found when P rufa, belonging to Lampyrinae was compared with H. papariensis and L. lateralis, both belong-ing to Luciolinae, confirming the current taxonomic status of the species. In the PAUP and PHYLIP analyses with 165 rRNA data, grouping of the two geographic samples of H. papariensis with H. tsushimana validate the use of generic name, Hotaria. Nevertheless, lack of sister-group relationship of the two geographic samples of H. papariensis renders further investigation on this group . Although the Korean and Japanese L. lateralis formed a strong monophyletic group, a substantial genetic differentiation was detected between them (2.9% of 165 rRNA gene sequence divergence). Finally, the geographic samples of Korean p. rufa strongly formed a group with Japanese p. rufa, warranting the use of generic name, Pyrocoelia, but the genetic distance observed between the Cheju-Island individual and all others requires further investigation on this subject. Summarized, this study supports the current taxonomic status of the Korean fireflies in that each respectively formed a strong monophyletic group with its own species or genus.

• Asian Journal of Turfgrass Science
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• v.19 no.2
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• pp.115-123
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• 2005

The effect of sodium hypochlorite treatment on the germination of cattail (Typha spp.) seeds was investigated in growth chambers maintained on a 14-h photoperiod with various temperatures and light intensities. Germination rates of seeds were, in general, enhanced by the increase of light intensity and temperature regardless of cattail species. Seeds of T. oreientalis had 4.3, 13.0 and $7.3\%$ germination at temperatures of 20, 25 and $30^{circ}$C, respectively, under light intensity of 7.5${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. T. angustata showed higher germination rate, thus, 10.7, 22.7 and $50.7\%$ under same temperature and light environment. Under high light intensity of 79.5${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, the germination rates of T. oreintalis and T angustata were $78.3\%$ and $88.7\%$ at $30^{circ}$C, respectively. Scarification of seeds with NaOC1 ($4\%$, available chlorine) increased germination rate in both species, especially even at low temperature of $20^{circ}$C. Germination speed was also enhanced by NaOC1 treatment. High light intensity further increased the germination rate. When NaOC1 treated seeds were sowed on the soil surface in plastic house, the seedling emergence was nearly $100\%$. Untreated seeds of T. oreintalis and T. angustara showed 40 and $50\%$, respectively, in germination under same condition. However, when the depth of soil covering was over 1.0 cm, seedling emergence was retarded more than 1 month. On the process of seedling development, emergence of mesocoty1 occurred firstly and after than primary root and first leaf were developed on the end of elongated mesocotyl. These results suggest that the promotion of seed germination by NaOC1 pretreament may be induced from the increase of light absorptivity as well as water permeability through scarifying and bleaching the seed coat.

• Korean Journal of Agricultural Science
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• v.9 no.2
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• pp.540-545
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• 1982

An energy metabolism study was conducted with two commercial strains of meat-type chickens, Hubbard and Cobb, and two egg strains, Hy Line and Korean-bred Hanhyup 325. The heat production of growing chickens from each strain were measured by the use of an open-circuit gravimetric respiration calorimeter. The data obtained from this study were summarized as fallows. 1. The average body weight of 9-wk-old Hubbard broilers reared in battery cages was 2,570g/bird. The average body weights of 9-wk-old Hy Line chicks and Hanhyup 325 were 777 and 748g/bird, respectively. 2. At 3 weeks of age, the Hubbard broiler chicks consumed two times the feed consumed by Hy Line chicks (54.6g VS. 26.7g/bird/day). These values increased to 151g and 57.2g/bird/day, respectively, at 8 weeks of age, indicating that the difference in feed intake between meat and egg-type chicks tends to increase as they grow older. In terms of water consumption, the 5-wk-old Hubbard broiler chicks drank $226m{\ell}/bird/day$ as compared with $58m{\ell}$ by Hy Line chicks. These values increased to 282 and $70m{\ell}$, respectively, at 8 weeks of age. 3. The excreta outputs of Hubbard broilers and Hy Line chicks were 18.7 and 6.1g DM/bird/day at 4 weeks of age, and 41.5 and 10.0g DM/bird/day at 8 weeks of age, respectively. 4. The energy metabolizability of broiler chicks were 75.4~77.1% compared to 75.0~83.5% by egg-type chicks. 5. The respiratory quotient (RQ) was between 0.78 and 0.97. There seems to be no difference in RQ between meat and egg-type chicks. The RQ tended to decrease when feed intake was low and vice versa. 6. Both meat and egg-type chicks produced $83.1{\sim}123.1Kcal/kg^{\frac{3}{4}}B.W./day$. The considerably low value of $83Kcal/kg^{\frac{3}{4}}B.W./day$ was obtained when the chicks were off the feed under the stressful conditions. The high value of 123.1Kcal was obtained when the chicken chamber temperature rose to $27{\sim}34^{\circ}C$.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.6
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• pp.757-767
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• 1995

This experiment was done to determine the optimum concentration of IAA for root development in plants regenerated from the callus culture of barley embryos. Two concentrations of 2,4-D, 3ppm and 5ppm selected as an optimum among five different concentrations in the previous experiment were used for callus induction and proliferation in this experiment. For callus induction, 3ppm of 2,4-D produced 35.6% in immature embryos and 4.4% in mature embryos, while 5ppm gave 33.8% in immature and 5.6% in mature embryos. Out of 320 immature embryos cultured, 111 embryos were induced to calli and 684 plants were produced from them, while only 16 embryos were induced to calli from 320 mature embryos and 92 plants were restored. The rates of callusing and plant regeneration were 34.7%, 214% in immature embryos and 5.0%, 28.7% in mature embryos, respectively. The average root lengths and root numbers of plants restored from callus at five different IAA concentrations of 0ppm, Ippm, 5ppm, l0ppm and 30ppm were 7.9mm, 3.6; 18.4mm, 5.2; 16.1mm, 3.9; 8.5mm, 3.5 and 6.4mm, 3.4, while plants directly obtained from mature embryos were 14.8mm, 4.9; 4.9mm, 3.6; 4.3mm, 3.1; 3.6mm, 2.6 and 3.2mm, 2.1, respectively. Therefore, 1ppm gave the best result for the root. promotion in callus, whle 0ppm, a control, gave the largest root developmemt in embryos. High concentration of lAA(30ppm) in callus and any exogeneous supplement of lAA in embryos negatively affected to the root lengths and root numbers. Genotypic effect was also observed in given four varieties, Bruce, Klages, Olbori and Albori. For chromosome doubling, when 0.1% colchicine was applied on 428 plants under three different conditions such as air circulation, temperatures and growth stages, 319 plants of doubled haploids were obtained so that the rate was 74.5%

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.17 no.2
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• pp.112-119
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• 2012

This study investigated the diel and tidal distributions of the two mysids, Archaeomysis kokuboi and Acanthomysis nakazatoi, in relation to their growth stages in the sandy surf zone of Yongil Bay, located on the southeastern part of Korean Peninsula. Sampling was conducted with a sledge net at every two hours for almost 24 hours at three sites: water edge, water surface and sand bottom both in 1-m deep water areas. The abundance of Archaeomysis kokuboi juveniles was too low to count both in day and night samples. While there was no difference in immature A. kokuboi abundance between day and night in the bottom or water edge, that at the water surface was significantly higher at night than daytime. The abundance of A. kokuboi adults, especially of males, in the bottom was significantly higher in daytime than night and no individuals appeared to the water surface either day or night. In comparison, the abundance of Acanthomysis nakazatoi juveniles between day and night did not differ significantly at all the three sites, with the highest number being distributed in the bottom. The abundance of immatures between day and night also did not differ significantly and no individuals appeared to the water surface either day or night. The abundance of A. nakazatoi adults, especially females, in the bottom was significantly higher at night than daytime and there was no significant difference in abundance between day and night in the other sites. There was also no significant difference in abundances of the two species between ebb and flood tides, except for A. kokuboi immatures which appeared significantly more during the ebb tides at the water surface. Overall, the distribution of the two sympatric species, A. kokuboi and A. nakazatoi, was not the same in the sandy surf zone. Its difference seems to depend on their stages of growth, and the change in their abundance may be influenced more by diurnal rhythms than tidal effects. The population density of A. nakazatoi in the sandy surf zone was much higher than that of A. kokuboi, and relatively higher densities in all growth stages of the former were found in the sandy bottom ranging from juveniles to adults. These results indicate that A. nakazatoi has exceedingly better ability of sand burrowing even from the juvenile stage, and thus is an ecologically better adapted species in the sandy surf zone than another sympatric species, A. kokuboi.