• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.8
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• pp.1221-1226
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• 2003

High hydrostatic Pressure was applied to Foxtail Millet Yakju to investigate the effects of high pressure on inactivation of microorganisms and enzymes. Total bacteria, lactic acid bacteria and yeast in untreated Yakju were $1.5{\times}$10$^4$,1.9${\times}$10$^4$ and 1.4${\times}$10$^4$ CFU/mL, respectively. Total bacterial count was reduced to 4.1${\times}$l0$^2$ CFU/mL, while lactic acid bacteria and yeast were sterilized completely in Yakju heated at $65^{\circ}C$ for 15 min. Lactic acid bacteria and yeast decreased greatly with the increase of treatment pressure, and were sterilized completely in Yakju treated at more than 300 ㎫ for 10 min/$25^{\circ}C$. Total bacteria were not completely sterilized with pressurization of even 600 ㎫ at room temperature and reduced to 2 log cycle even at $65^{\circ}C$. Total bacteria decreased by 2∼3 log cycle with the increase of treatment time from 10 to 60 min at $25^{\circ}C$/300 ㎫. Pressurization of Yakju caused a partial inactivation of $\alpha$ -amylase and glucosamylase, and the activities of $\alpha$ -amylase and glucoamylase decreased by 18.1% and 21.1%, respectively at $25^{\circ}C$/600 ㎫/10 min. Activities of $\alpha$ -amylase and glucoamylase decreased with the increase of temperature, and 22.2% and 32.1% of the original activity were remained with the treatment at $65^{\circ}C$/300 ㎫/10 min, respectively. Enzyme activities decreased slightly with the increase of treatment time at $65^{\circ}C$/300 ㎫.

• Applied Biological Chemistry
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• v.36 no.4
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• pp.277-283
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• 1993

In order to brew foxtail millet wine, a folk wine of Cheju, properties of raw materials, optimum brewing conditions were inveatigated. Carbohydrate and crude fat content of glutinuous foxtail millet are 71.27% and 3.47%, respectively. Since the ratio of water to steamed millet and ethanol concentration of wine showed negative correlation, less than 250% water had to added to steamed millet to maintain ethanol concentration in wine above 13%, Sugar consumption and ethanol production increased rapidly for the first 2 days, and main fermentation was done in 4 days. Ethanol concentrations were $13.0{\sim}13.4%$ when foxtail millet was used, and they were $14.0{\sim}14.3%$ for the mixture substrates of 90% millet and 10% rice or barley. Organic acids in millet wine were lactic acid, malic acid and succinic acid. The residual carbohydrates after fermentation were mainly xylose and oligosaccharides. A trace of methanol was detected in millet wine. The content of fusel oil was low, while the concnetration of organic acids was high. Optimum conditions for millet wine-making were as follows. Glutinuous foxtail millet with 10% rice as fermentation source need to be soaked in water and steamed for enough time. Water was added to steamed millet with the ratio of 2 : 1. The resulting mixture was stmnultaneously saccharified and fermented by Aspergillus orzae and Saccharomyces cerevisiae IAM 4274 at $20^{\circ}C$ for a week. Millet wine was prepared after filtering fermented broth while pressing for a week.

• Applied Biological Chemistry
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• v.47 no.1
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• pp.85-91
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• 2004

In order to improve foxtail millet wine, a traditional Jeju cereal wine, fermentation characteristics of millet Yakju with different types of Nuruks prepared using isolated Aspergillus sp. and Rhizopus sp. were investigated. When the millet wine was brewed with the Nuruk prepared in this study, the combination ratio of wheat flour: barley : wheat bran : millet = 8 : 1 : 1 : 0 (pellet) showed the highest level of alcohol concentration, and a more favorable score than Kuksundang Nuruk in sensory evaluation. The main organic acids in millet wine were lactic and acetic acids, and the minor organic acids were fumaric, oxalic, citric and malic acids. Analysis of sugar compositions showed that glucose, arabinose, and maltose were present in decreasing order, and that xylose was also detected. Flavor components of millet wine were mainly iso-amyl, iso-butyl and n-propyl alcohols. Ethylacetate and acetadehyde were also detected. The contents of iso-butyl and n-propyl alcohols were higher in the millet wine prepared with Kuksundang Nuruk than those prepared with other Nuruks.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.2
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• pp.277-284
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• 2005

Micro-filtration (MF) or ultra-filtration (UF) system with hollow-fiber cartridge was introduced in order to improve the Quality level of commercial foxtail millet Yakju, which has an off-flavour and/or undesired colour after the thermal treatment. The filtration effects of cartridges such as MF (0.65, 0.45, 0.2, 0.1 $\mu$m) and UF (500 K dalton) were investigated. The physicochemical and sensory characteristics of the Yakju were then evaluated during the 6 months storage at room temperature. The exclusion ability of microorganism in samples was confirmed in all cartridges, but 0.45 pm MF-cartridge was suitable in the Yakju manufacture due to its superior filtration rate and efficiency. Changes in reducing sugar and colour difference of foxtail millet Yakju untreated or treated by heat ($65^{\circ}C$${\times}$10 min) were observed during the storage; after 6 months the L-value of thermal-treatment sample was decreased and its b-value, however, significantly increased so that its color became dark, in comparison to non-thermal treatment sample. This decrease of reducing sugar is assumed that color change is associated with non-enzymatic browning reaction. Sensory Quality of foxtail millet Yakju produced by non-thermal treatment was better than that of thermal treatment.

• Proceedings of the Korean Nutrition Society Conference
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• 2001.05a
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• pp.242.2-242
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• 2001

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.176.1-176
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• 2003

제주지역 양조산업의 산업화 기술 개발의 일환으로 제주지역의 대표적인 전통 좁쌀 약주 제품에 대하여 유통 과정 중에 발생하는 침전 형성을 방지하는 기술을 개발하기 위하여 여과 공정 개선에 관한 연구를 실시하고 개선 방안을 제시하였다. 각종 공극 크기의 유리 막 여과지와 중공사막 카트리지 여과 연구 결과, 이 좁쌀 약주 제품에서 대부분이 침전 형성 가능한 입자들은 1.2$\mu\textrm{m}$여과 매질 공극 크기에 의한 여과에 의하여 제거 되여 보통의 소비에 문제가 되는 침전은 잘 않되는 것으로 조사되었다. 현재 제품 제조 현장에서 사용되고 있는 l$\mu\textrm{m}$ 공극 크기 원통형 정밀 여과기인 경우 이론적으로는 합당하나 여과 매질에 대한 산업 인 공극 크기에 대한 오차 한계인 $\pm$20-30% 때문에 일부제품에서 침전 발생하는 것으로 분석되었다. 또한 4$^{\circ}C$와 실온을 48시간씩 반복하면서 3개월간 저장 중 유리 막 여과지인 경우는 0.7$\mu\textrm{m}$ 공극크기의 여과에서도 세밀한 관찰에 의하여 침전형성이 관찰되었으나 중공사막인 경우에는 0.45$\mu\textrm{m}$에서도 전연 침전형성이 관찰되지 않았다. 따라서 완벽한 침전 형성 방지를 위해서는 최종 여과공정에 중공사막 여과 시스템 도입이 효과적이며, 설계 기준은 0.45$\mu\textrm{m}$ 공극과 내경 1mm정도의 중공사막을 유효 여과 면적 1$m^2$정도를 가지도록 하는 중공사막 여과 시스템인 경우 여과 능력은 약400ι/hr로 계산되었다.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.199.1-199
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• 2003

제주민속주인 좁쌀약주의 제조를 위하여 전국에서 수집된 35종의 누룩으로부터 우수 곰팡이와 효모를 분리하였다. 수집된 누룩의 균수는 곰팡이가 6.4$\times$105~107개, 효모는 1.4$\times$104~107개로 나타났고, 이 중에서 곰팡이 169균주, 효모 103균주를 분리하였으며 전분당화력이 좋은 곰팡이 16균주와 효모 1균주를 선발하였다. 이 중에서 전분당화 균주의 효소활성을 분석한 결과 Aspergillus속으로 동정된 A8-3이 glucoamylase 활성, 액화력, xylanase 활성이 가장 높았고, Rhizopus속으로 동정된 B23-3은 당화력이 가장 우수하였다. 우수효모를 선발하기 위하여 pH, 무게 감량, 내당성, 내알코올성 등을 측정한 결과, Saccharomyces속인 A10-4가 가장 우수하였다. 같은 원료비율로 만든 누룩에 우수균주를 접종하였을 때, 단일 균주를 처리할 때보다 A8-3과 B23-3인 두 균주를 혼합하여 처리한 경우가 당화력이 높게 나타났다. 누룩을 원반형의 누룩과 팰릿(개량형) 형태로 만들어 혼합종균 배양액을 접종한 후 당화력을 측정한 결과, 비슷한 당화력을 나타내었다. 개량형 누룩을 사용하여 양조하는 경우, 좁쌀주 양조에 발효효율을 높일 수 있을 것으로 판단되었다. 수집된 누룩은 수분이 10~13%, 총당은 55~70%, 조단백질은 10~18% 조지방은 0.2~l.0%, 회분은 1.8~2.1%이었다. 본 연구에서 제조한 누룩은 수분이 12~15%, 총당은 61~7l%, 조단백질은 15~20%, 조지방은 0.4~1.5%, 회분은 1.1~1.5%이었다.

• Food Science and Preservation
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• v.10 no.4
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• pp.482-487
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• 2003

The filtration effects of glass membrane filters and hollow fiber membranes with different pore size to prevent the haze of a commercial foxtail millet Yakju were investigated. The most particles(haze precusors) present in the Yakju were removed by using 1.2 $\mu\textrm{m}$ glass membrane filter. In case of using 0.45$\mu\textrm{m}$ hollow fiber membrane system, the permeate flux on the Yakju filtration was calculated to 342.8 lmh. During the 3 month storage at 4$^{\circ}C$ and room temperature for each 48 hrs in turn, alittle fine haze was observed from the Yakju even if it was filtered by using 0.7 $\mu\textrm{m}$ glass membrane filter. Anything of haze was, however, observed from the Yakju filtered by using all of hollow fiber membranes. Therefore, the 0.45$\mu\textrm{m}$ hollow fiber membrane system was effective as a final filtration process of the Yakju.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.199.2-199
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• 2003

제주민속주인 좁쌀약주의 제조를 위하여 전국에서 수집된 35종의 누룩으로부터 분리하여 선발한 균주를 사용하여 균주와 원료배합비율에 따른 누룩을 제조한 후 양조과정에서의 발효특성을 검토하였다. 좁쌀주의 유기산 및 유리당의 분석결과 유기산은 lactic acid와 acetic acid가 대부분이었고, 이외에 citric acid, oxalic acid, tartaric acid, malic acid, fumaric acid도 일부 검출되었다. 유리당은 glucose와 arabinose, maltose가 많은 함량을 나타내었고, 이외에 xylose도 일부에서 검출되었다. 향기성분은 i-amyl alcohol, i-butyl alcohol, n-propyl alcohol 등이 주를 이루고 있었으며, 이외에 ethylacetate, acetaldehyde가 검출되었다. i-buthyl alcohol과 n-propyl alcohol은 대조구인 Dl이 다른 처리구보다 높게 나타났고, i-amyl alcohol은 K5 처리구가 높게 나타났다. 본 연구를 통하여 우수균주로 분리하여 제조한 누룩을 사용하여 좁쌀주를 양조하는 경우, 품질을 유지하면서 수율을 향상시킬 수 있었다.

• Korean Journal of Food Science and Technology
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• v.24 no.1
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• pp.101-106
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• 1992

Vie investigated the clarification effects of foxtail millet wine, a traditional wine in Cheju island, by centrifugation, protease treatment and ultrafiltration(UF). It was difficult to remove completely cloudy substances in foxtail millet wine only by centrifugation. With protease concentration of $5{\times}10^{-4}%(w/v)\;at\;35^{\circ}C$, for 2 hrs, foxtail millet wine was clarified effectively. Papain and bromelain appeared similar effects to the clarification of wine, but ficin was inferior to those. Ultrafiltration with pore size $100K\;and\;0.22{\mu}m$ membranes had appeared better clarification effects than the best result of enzyme treatment, and then was considered as a simple and economic method.