• Horticultural Science & Technology
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• v.34 no.2
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• pp.342-353
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• 2016

This study was initiated to evaluate green color retention under three different soil systems. Several turfgrasses were evaluated in multi-layer, USGA, and mono-layer systems. Turfgrass entries were comprised of three cultivars of Korean lawngrass (Zoysia japonica Steud.) as warm-season grass (WSG) and three blends and three mixtures of Kentucky bluegrass (KB, Poa pratensis L.), perennial ryegrass (PR, Lolium perenne L.), and tall fescue (TF, Festuca arundinacea Schreb.) as cool-season grass (CSG). Significant differences were observed in visual turf color and green color retention among soil systems and turfgrasses. Both the multi-layer and USGA systems were highly associated with better color ratings and longer color retention, as compared with the mono-layer system. Seasonal variation of visual turf color greatly occurred from late December to early spring. CSG exhibited longer color retention than did WSG. The latter maintained green color for approximately 6 months, regardless of the soil system. Spring green-up of Korean lawngrass occurred from early to middle May, while it underwent discoloration from late October to early November. Among the CSGs green-up occurred between early March and early April and leaf color was maintained until middle December to early February. Therefore, the CSGs were green for 8.5 to 11 months, depending on turfgrass and soil system. The mean period of green color duration across all soil systems was approximately 10-11, 9-10 and 8.5-9.0 months for PR, KB and TF, respectively. As for the CSG mixtures, the greater the proportion of PR, the longer the green color retention, while the higher the proportion of TF, the shorter the color retention. There was greater variation in green color duration among the CSGs than the WSGs. Across soil systems, color retention differences of 2 to 6 days were observed for the Korean lawngrass, but 7 to 36 days for the CSGs. These results demonstrate that green color retention varied greatly according to soil systems and also among turfgrasses. Selections of turfgrass and soil system should be made using a concept-oriented approach, when establishing garden, park, soccer field, golf course and other sports field. Information obtained in this study can be used to select soil systems and turfgrasses based on the expected degree of leaf color retention.

• Horticultural Science & Technology
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• v.29 no.4
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• pp.366-373
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• 2011

To analyze the genetic relationships among Korean potato cultivars and to develop cultivar identification method using DNA markers, we carried out genotyping using simple sequence repeats (SSR) analysis and developed multiplex-SSR set. Initially, we designed 92 SSR primer combinations reported previously and applied them to twenty four Korean potato cultivars. Among the 92 SSR markers, we selected 14 SSR markers based on polymorphism information contents (PIC) values. PIC values of the selected 14 markers ranged from 0.48 to 0.89 with an average of 0.76. PIC value of PSSR-29 was the lowest with 0.48 and PSSR-191 was the highest with 0.89. UPGMA clustering analysis based on genetic distances using 14 SSR markers classified 21 potato cultivars into 2 clusters. Cluster I and II included 16 and 5 cultivars, respectively. And 3 cultivars were not classified into major cluster group I and II. These 14 SSR markers generated a total of 121 alleles and the average number of alleles per SSR marker was 10.8 with a range from 3 to 34. Among the selected markers, we combined three SSR markers, PSSR-17, PSSR-24 and PSSR-24, as a multiplex-SSR set. This multiplex-SSR set used in the study can distinguish all the cultivars with one time PCR and PAGE (Polyacrylamide gel electrophoresis) analysis and PIC value of multiplex-SSR set was 0.95.

• Horticultural Science & Technology
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• v.31 no.3
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• pp.259-268
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• 2013

This study was carried out to evaluate the visual turfgrass's color quality, winter color, and spring green-up under three different soil systems and to make a practical use for sports turf design and construction. Several turfgrasses were evaluated in multi-layer, USGA and mono-layer systems. Turfgrass entries in the study comprised of 3 cultivars from Korean lawngrass (Zoysia japonica Steud.) of typical warm-season grass (WSG) and 3 blends and 3 mixtures from Kentucky bluegrass (KB, Poa pratensis L.), perennial ryegrass (PR, Lolium perenne L.), and tall fescue (TF, Festuca arundinacea Schreb.) of cool-season grass (CSG). Significant differences were observed in the turfgrass's color quality, winter color, and spring green-up in the study. Seasonal variation of visual turf color greatly occurred according to soil systems and turfgrasses. Multi-layer and USGA systems were highly associated with better visual color ratings, as compared with mono-layer system. Regardless of soil system, visual turf color in all entries was better from spring to fall than in winter. Great color differences were observed during a period of early December to early spring. CSG produced a better color quality over WSG in any soil system. Overall color ratings for CSG were KB > PR > Mixtures > TF. As for a winter color, its ranking was USGA > multi-layer > mono-layer system. No difference was found in winter among cultivars of Korean lawngrass, being completely brown, but great differences among CSG. Rated best for winter color was PR, followed by CSG mixtures, KB and finally TF in order. It was generally conceded that fast green-up in spring was greatly related with multi-layer over mono-layer system and also CSG over WSG. Among CSG, TF had a fastest green-up. PR was also fast in green-up, but poor in color uniformity. KB, however, was the slowest due to shallow rooting system, when compared with other CSGs. These results demonstrate color differences were greatly variable according to soil systems and also among turfgrass species. A precise decision should be made in selecting turfgrass species and soil system. Multi-layer and USGA systems were considered as the suitable one for turfgrass color quality, winter color and spring green-up. It is a great necessity to combine proper soil system, right turfgrass species, and appropriate mixing rates by a concept-oriented approach, when establishing garden, parks, soccer field, and golf courses and so on.

• Applied Chemistry for Engineering
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• v.29 no.3
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• pp.283-288
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• 2018

As the use of environmentally friendly and non-disease natural pigments grows, various methods for extracting natural pigments have been studied. The natural color was extracted from parsley, a vegetable ingredient containing natural dyes. Target color codes of green series of natural dyes extracted as variables #50932C (L = 55.0, a = -40.0, b = 46.0) were set with the pH and temperature of extracted natural color coordinates (of the extracted), and the quantitative intensities of natural dyes were analyzed. During the colorimetric analysis predicted by the reaction surface analysis method, a color coordinate analysis was conducted under the optimal conditions of pH 8.0 and extraction temperature of $60.9^{\circ}C$. Under these conditions, predicted figures of L, a, and b were 55.0, -36.3, and 36.8, respectively, while actual experimental ones confirmed were 69.0, -35.9, and 31.4, respectively. In these results, the theory accuracy and actual error rate were confirmed to be 73.0 and 13.8%, respectively. The theoretical optimization condition of the color difference (${\Delta}E$) was at the pH of 9.2 and extraction temperature of $55.2^{\circ}C$. Under these conditions the predicted ${\Delta}E$ figure was 12.4 while the experimental one was 13.0. The difference in color analysis showed 97.5% of the theoretical accuracy and 4.5% of the actual error rate. However, the combination of color coordinates did not represent a desired target color, but rather close to the targeted color by means of an arithmetic mean. Therefore, it can be said that when the reaction surface analysis method was applied to the natural dye extraction process, the use of color coordinates as a response value can be a better method for optimizing the dye extraction process.

• Journal of Life Science
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• v.26 no.3
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• pp.275-281
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• 2016

Glycan modification is important in pharmaceutical industry. Especially, sialic acid affects the bioactivity and stability of medicine. Milk of pig has been used as bioreactor to produce various pharmaceutical proteins. Therefore, it is necessary to modify the glycan chain in pig mammary grand. β-1,4-N-Acetylglucosaminyltransferase A (pMGAT4A) is one of the essential enzymes for increase of sialic acid content, but pig MGAT4A is unclear. In this study, the pMGAT4A was identified and characterized. The pMGAT4A has 1638 nucleotides encoding 535 amino acids and type II membrane topology, which is one of the common features in many glycosyltransferases. The gene was strongly expressed in liver and mammary gland, whereas was weakly expressed in small intestine, stomach and bladder. For functional test, HA-tagged MGAT4A was over-expressed in porcine kidney (PK-15) cell line. Forced expression of pMGAT4A gene was identified by qPCR, and we identified that pMGAT4A is located in Golgi complex by co- staining with HA antibody and BODIPY TR ceramide. In addition, we identified the increase of mannose-β-1,4-N-acetylglucosamine structure by ELISA and immunofluorescence using Datura stramonium agglutinin (DSA), which recognizes mannose-β-1,4-Nacetylglucosamine. Through the specific activity analysis, we showed that pMGAT4A modified bi-antennary to tri-antennary. This event affects sialic acid content. Therefore, we thought that over-expression of pMGAT4A will be necessary in pig mammary grand for improved medicine.

• Journal of Animal Science and Technology
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• v.44 no.2
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• pp.239-250
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• 2002

Evaluation works of oat in mixture with rye on forage yield have not been reported. The objective of this study was to determine yield and quality of forage mixture as affected by maturity of rye cultivar and oat-rye seeding rate at Suweon from 1999 to 2000. The experiment was arranged in a split plot design with three replications. Main plots consisted of maturity of rye. such as early(cv. Koolgrazer) and late(cv. Kodiak)maturing cultivars. Sub-plots consisted of seeding rate (T1: Oat 2000 and rye 0kg/ha, Ts: Oat 150 and rye 40kg/ha, T3: Oat100 and rye 80kg/ha, T4: Oat50 and rye 120kg/ha. and T5: Oat0 and rye 160kg/ha). Crude protein(CP) content of oat-rye mixture harvested in the fall was not influenced by maturity of rye cultivar, but that of oat-rye mixture was increased from 13.6 to 19.3% as the seeding rate of rye increased(P<0.05), however, maturity of rye cultivar significantly affected CP content of oat-rye mixture in the spring(P<0.01). Acid detergent fiber(ADF) content of oat-rye mixture harvested in the spring was not significantly affected by rye cultivar, but the ADF was decreased from 27.8 to 20.7% as the seeding rate of rye increased(P$<$0.01). When rye was harveste in the spring, ADF content of late maturing cultivar 'Kodiak' was shown as 28.0%. This was lower than that of early maturing cultivar 'Koolgrazer' which was shown as 35.8%(P$<$0.01). Among treatments, neutral detergent fiber(NDF) and in vitro dry matter digestibility(IVDMD) of oat-rye mixture showed a similar trend made on ADF. In this experiment, the highest forage yield (12.356kg/ha) was obtained from early maturing rye cultivar and seeding rates of 100kg/ha of oat and 80kg/ha of rye mixture. A significant interaction between maturity of rye cultivar and seeding rate was found(P$<$0.01). The above results indicate that an early maturing rye cultivar at the seeding rate of 100kd/ha in mixtures with 80kg/ha of oat could be recommended as a succeeding cropping system after corn for silage.

• Journal of Animal Science and Technology
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• v.44 no.2
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• pp.251-260
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• 2002

The corn (Zea mays L) planting date in a double-cropping system is delayed until mid-May due to delayed rye harvest on May. This experiment was conducted to determine the optimum harvesting time for high yield and the best quality of silage corn at late planting date after harvesting winter rye. Corns were planted on 21 May and harvested at eight different maturity stage at Seoul National University Experimental Livestock Farm, Suwon in 1997. Maturities were B (blister; 16 days after silking), M (milk; 20 days), LM (late milk; 24 days), SD (soft dough; 28 days), ED (early dent; 33 days), FD (full dent; 38 days), LD (late dent; 44 days) and PM (physiological maturity; 53 days) stages. The percentage of whole plant dry matter (DM) showed optimum range for silage making (29.0 to 38.5%) when corn plant was harvested at between ED and LD stages. Maximum whole plant DM (14,831 kg/ha) and total digestible nutrients (TDN) yields (10,675 kg/ha) reached at full dent stage. The percentage of whole plant acid detergent fiber (ADF) was decreased from 35.4 to 22.1%, and that of neutral detergent fiber (NDF) was also decreased from 63.8 to 46.0% as harvest stage progressed. These changes in chemical compositions were associated with changes in plant part composition. A progressive increase in total ear, and the decrease in stover portion in the plant were observed with advance in harvest stage. Calculated on net energy for lactation (NEL) and TDN values based on ADF percentage of stover plant decreased by ED stage and then increased by PM stage. But NEL and TDN values of ear and whole plant increased as harvest stage progressed. While in vitro dry matter digestibility of stover was decreased from 61.1 to 49.7%, whole plant was increased from 58.3 to 65.7% as maturity advanced (P$<$0.05). The results of this study indicate that corn can be harvested for silage at full (1/2 milkline) and late dent (2/3 milkline) stages for maximum yield and optimum quality at late planting. And days after silking at late planting was 38 and 44 days.

• Journal of Animal Science and Technology
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• v.51 no.3
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• pp.201-206
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• 2009

Seventeen porcine microsatellite (MS) markers recommended by the EID+DNA Tracing EU project, ISAG and Roslin institute were selected for the use in porcine individual and brand identification. The MSA, CERVUS, FSTAT, GENEPOP and API-CALC programs were applied for calculating heterozygosity indices. By considering the hetreozygosity value and PCR product size of each marker, we established a MS marker set composed of 13 MS markers (SW936, SW951, SW787, S00090, S0026, SW122, SW857, S0005, SW72, S0155, S0225, SW24 and SW632) and two sexing markers. The expected probability of identity among genotypes of random individuals (PI), probability of identity among genotypes from random half sibs ($PI_{half-sibs}$) and among genotypes of random individuals, probability of identity among genotypes from random sibs($PI_{sibs}$) were estimated as $2.47\times10^{-18}$, $6.39\times10^{-13}$ and $1.08\times10^{-8}$, respectively. The results indicate that the established marker set can provide a sufficient discriminating power in both individual and parentage identification for the commercial pigs produced in Korea.

• Journal of Bio-Environment Control
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• v.21 no.1
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• pp.66-73
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• 2012

This study was conducted to develope new indoor plants and to investigate the effects of light intensity on the growth of $Distylium$ $racemosum$, $Osmanthus$ $heterophyllus$ and $Damnacanthus$ $indicus$ under 100 lux, 1,000 lux and 2,500 lux fluorescent lighting for six months in the environment-controlled growth chambers. 1. $Distylium$ $racemosum$ : Most of $Distylium$ $racemosum$ under 100 lux light intensity were blighted in two months, whereas it was 100% of survival under 1,000 lux and 2,500 lux after six months. Plant height, number of leaf, leaf width and leaf length became higher as light intensity increased. The plants maintained under 2,500 lux showed the greatest plant height and leaf number. 2. $Osmanthus$ $heterophyllus$ : $Osmanthus$ $heterophyllus$ under 100 lux light intensity were blighted in two months, whereas it was 100% of survival under 1,000 lux and 2,500 lux after six months. However, under 1,000 lux, it paused plant height and was not increased in leaf number any more. A plant growth status showed the highest value under 2,500 lux in all conditions. 3. $Damnacanthus$ $indicus$ : $Damnacanthus$ $indicus$ was defoliated and blighted under 100 lux light intensity in two months, whereas it was grown properly with 1,000 lux or above. However, the growth under 2,500 lux of $Damnacanthus$ $indicus$ was superior to other treatments. But, $Damnacanthus$ $indicus$ under 1,000 lux after 6 months was more favorable chlorophyll contents, leaf length and leaf width than 2,500 lux. As increasing slightly of chlorophyll contents and leaf growth under 1,000 lux, $Damnacanthus$ $indicus$ could be utilized highly to the indoor ornamental plant.

• Journal of Bio-Environment Control
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• v.20 no.3
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• pp.189-196
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• 2011

The objective of this study was to investigate the effects of light intensity and electrical conductivity (EC) of nutrient solution during short day treatment in an ebb and flow systems on the growth and nutrient uptake of potted Kalanchoe blossfeldiana 'Rako' and the nutrient accumulation of growing medium. Nutrient concentrations in the growing medium were also analyzed to investigate the accumulation rates of macro-nutrients such as T-N, P, K, Ca, and Mg, respectively. To achieve the objectives, plants were fed with a nutrient solution with 1.2, 1.8, or $2.4dS{\cdot}m^{-1}$ under three daily photosynthetic photon flux (PPF) of 4.26, 5.51, or $9.75mol{\cdot}m^{-2}{\cdot}d^{-1}$. Both light intensity and EC of nutrient solution significantly influenced the crop growth. The elevation of PPFs resulted in the increase of plant growth. For each light condition, plant growth, such as dry and fresh weight and leaf area, was the highest when the electrical conductivity of nutrient solution was controlled to $2.4dS{\cdot}m^{-1}$. However, growth was acceptable in the EC ranges from 1.8 to $2.4dS{\cdot}m^{-1}$. Both light intensity and EC of nutrient solution significantly influenced the uptake of nutrients in the solution tanks and the accumulation of nutrients in the growing medium. As the EC of nutrient solution was elevated, the absorption rates of $NO_3^-$, $PO_4^{-3}$, $K^+$, and $Mg^{2+}$ by crops and accumulation of those in growing medium increased, but the light intensity did not significantly influence the absorption rates. Based on the above results, the regression models were suggested for anticipating the macro-nutrient accumulations in growing medium.