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Development of Multiplex Microsatellite Marker Set for Identification of Korean Potato Cultivars  

Cho, Kwang-Soo (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration)
Won, Hong-Sik (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration)
Jeong, Hee-Jin (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration)
Cho, Ji-Hong (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration)
Park, Young-Eun (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration)
Hong, Su-Young (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration)
Publication Information
Horticultural Science & Technology / v.29, no.4, 2011 , pp. 366-373 More about this Journal
Abstract
To analyze the genetic relationships among Korean potato cultivars and to develop cultivar identification method using DNA markers, we carried out genotyping using simple sequence repeats (SSR) analysis and developed multiplex-SSR set. Initially, we designed 92 SSR primer combinations reported previously and applied them to twenty four Korean potato cultivars. Among the 92 SSR markers, we selected 14 SSR markers based on polymorphism information contents (PIC) values. PIC values of the selected 14 markers ranged from 0.48 to 0.89 with an average of 0.76. PIC value of PSSR-29 was the lowest with 0.48 and PSSR-191 was the highest with 0.89. UPGMA clustering analysis based on genetic distances using 14 SSR markers classified 21 potato cultivars into 2 clusters. Cluster I and II included 16 and 5 cultivars, respectively. And 3 cultivars were not classified into major cluster group I and II. These 14 SSR markers generated a total of 121 alleles and the average number of alleles per SSR marker was 10.8 with a range from 3 to 34. Among the selected markers, we combined three SSR markers, PSSR-17, PSSR-24 and PSSR-24, as a multiplex-SSR set. This multiplex-SSR set used in the study can distinguish all the cultivars with one time PCR and PAGE (Polyacrylamide gel electrophoresis) analysis and PIC value of multiplex-SSR set was 0.95.
Keywords
cluster analysis; polymorphism information contents (PIC); Solanum tuberosum; simple sequence repeat (SSR);
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