• Korean Journal of Ichthyology
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• v.22 no.2
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• pp.115-120
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• 2010

Genetic variation and population structure of the slender bitterling Acheilognathus lanceolatus of Korea (the Han, Geum, Dongjin, Seomjin and Nakdong Rivers) and Japan (the Katsura River) were assessed by amplified fragment length polymorphism (AFLP) analysis. Five combinations of selective primers generated 345~374 DNA fragments, of which 55~131 were polymorphic. The Nakdong River population had the highest genetic diversity and the Han River population had the lowest genetic diversity. Dendrogram based on the distance matrix revealed that individuals from each population consistently clustered together and bifurcated into two distinct clades (or population groups) composed of the Han, Geum, Dongjin and Seomjin River populations and of the Nakdong and Katsura River populations, supported with high bootstrap values. The pairwise genetic differentiation ($F_{ST}$) estimates showed that the six populations were genetically well differentiated (P<0.01). The analysis of molecular variance (AMOVA) after partitioning the six populations into two population groups revealed very strong biogeographic structuring between them with 25.49% of total variance (P<0.01). Taken together, the AFLP markers clearly divided six A. lanceolatus populations into two population groups.

• Investigative Magnetic Resonance Imaging
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• v.16 no.1
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• pp.47-54
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• 2012

Purpose : This study was performed to evaluate the characteristics of rat mesenchymal stem cells (RMSCs) transduced with human ferritin gene and investigate $in$ $vitro$ MRI detectability of ferritin-transduced RMSCs. Materials and Methods: The RMSCs expressing both myc-tagged human ferritin heavy chain subunit (myc-FTH) and green fluorescence protein (GFP) were transduced with lentiviurs. Transduced cells were sorted by GFP expression using a fluorescence-activated cell sorter. Myc-FTH and GFP expression in transduced cells were detected by immunofluorescence staining. The cell proliferative ability and viability were assessed by MTT assay. The RMSC surface markers (CD29+/CD45-) were analyzed by flow cytometry. The intracellular iron amount was measured spectrophotometically and the presence of ferritin-iron accumulation was detected by Prussian blue staining. $In$ $vitro$ magnetic resonance imaging (MRI) study of cell phantoms was done on 9.4 T MR scanner to evaluate the feasibility of imaging the ferritin-transduced RMSCs. Results: The myc-FTH and GFP genes were stably transduced into RMSCs. No significant differences were observed in terms of biologic properties in transduced RMSCs compared with non-transduced RMSCs. Ferritin-transduced RMSCs exhibited increased iron accumulation ability and showed significantly lower $T_2$ relaxation time than non-transduced RMSCs. Conclusion: Ferritin gene as MR reporter gene could be used for non-invasive tracking and visualization of therapeutic mesenchymal stem cells by MRI.

• Journal of Korean Society of Environmental Engineers
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• v.36 no.3
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• pp.153-161
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• 2014

The purpose of this study is to evaluate effect of heavy metals (i.e., $Cu^{2+}$, $Zn^{2+}$, $Cr^{6+}$, $Cd^{2+}$, $Hg^{2+}$, and $Pb^{2+}$) to toxicity of Triclosan as binary mixture. The individual toxicity and combined toxic effects of Triclosan with heavy metals were evaluated by Vibrio fischeri assay. In individual toxicity, the $Hg^{2+}$ was found to be most toxic followed by Triclosan, $Pb^{2+}$, $Cr^{6+}$, $Cu^{2+}$, $Zn^{2+}$, and $Cd^{2+}$, respectively. To evaluate combined toxic effect, correlation analysis of 'predicted value' calculated by Concentration addition (CA) model and Independent action (IA) model with 'experimental value' were performed based on the toxicity of individual compound. As a result, all of the combinations showed that IA model were more correlated with experimental value than CA model. On the basis of the median effect concentration of combination ($EC_{50mix}$) predicted by IA model, experimental $EC_{50mix}$ of Triclosan + Cu, Triclosan + Zn, Triclosan + Pb, Triclosan + Hg, Triclosan + Cd, and Triclosan + Cr were 191%, 226%, 138%, 137%, 209%, and 138% of $EC_{50mix}$ predicted by IA model, respectively, indicating that all of the combinations produced antagonistic effect.

• Cartoon and Animation Studies
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• s.33
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• pp.55-78
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• 2013

The issues on the representation of digital images, the uncanny is not considered aesthetically but must be overcome. It seems that the future of visual media using digital images, depends on the development and advancement of the digital technology. However, the Brothers Quay regarded as unique and experimental animators, focus their attention towards the past rather than the future. They trace back to the origin of the media, reproducing, manipulating, and representing gestures in oder to restore the shock and thrill delivered in film and animation. The Brothers Quay explore the uncanny of gestures through the mechanism of medium. They work in a wide range of genres, from animation, live action to dance film. The creaking spasms, which create the feeling that the movement does not belong to this world, and the strange gestures, reveals the flicker of the still projection and make audiences recognize gestures in the true meaning of the word. While they animate live actors and dead objects like automata, the hidden desires are revealed over screen. In this paper, focusing on the works of Brothers Quay, I tried to explore the uncanny of gesture based on Freud's and Jentsch' theory of uncanny.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.4 no.2
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• pp.155-160
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• 2001

Purpose: Helicobacter pylori has been known to have diverse vacA allelic types. The purpose of the study was to identify vacA diversity in Korea and design new primers for signal sequence alleles indigenous to Korea. Methods: Fifty antral biopsy specimens, which had been proven to be H. pylori-positive, were examined for vacA status; signal sequence and mid-region. After PCR amplification and DNA sequencing, vacA alleles of Korean H. pylori strains were compared with those from other countries. Results: Among Korean H. pylori strains vacA alleles with all combinations of signal sequence and mid-region were found, with the exception of s1b or s2. vacA genotype s1c/m1 was predominant in Korea. We found that GGGAGCGTTR in s1a and GGGGYTATTG in s1c were the indigenous sequences to Korea and constructed the new Korean specific primers for the vacA signal sequence; VASK-F, VASK-R, S1AK-F, and S1CK-F. Conclusion: This study showed that s1c/m1 is the predominant type of vacA allele in Korea. We designed new primers for the vacA signal sequence.

• Journal of Chest Surgery
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• v.43 no.6
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• pp.565-575
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• 2010

Background: Various decellularization methods have been studied in order to develop tissue graft which is less immunogenic and more durable. This study was performed to investigate the physico-dynamic and histological effect of trypsin pretreatment on decellularization protocols. Material and Method: Two groups of bovine pericardium specimen each underwent decellularization process based on SDS and Triton X-100 or N-lauroylsarcosinate and Triton X-100. Two more groups additionally underwent pretreatment with 0.1% Trypsin/0.1% EDTA. After decellularization process, mechanical tensile strength was tested, then biomechanical test of permeability and compliance was tested before and after fatigue test. Light microscopy and electron microscopy was performed to observe histological findings. Result: There was no difference in mechanical tensile strength between groups, but permeability and compliance was decreased in trypsin pretreated groups. Light microscopic and electron microscopic findings revealed damage of the extracellular matrix in trypsin pretreated groups and in groups which underwent the fatigue test also. Conclusion: Trypsin pretreatment in decellularizing process of bovine pericardium damages extracellular matrix and increases permeability and compliance of the bovine pericardium, but did not decrease tensile strength. Further studies are needed to use enzymatic treatments in decellularization protocols.

• Journal of Korea Technology Innovation Society
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• v.16 no.3
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• pp.650-671
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• 2013

Products elicit the consumer's purchasing behavior by satisfying their needs and are cognized as the combination of various needs attributes. Also R&D is referred as a series of technical development activities to meet the consumer's needs attributes. In particular, in the market-oriented R&D era, it could obtain the legitimacy by developing the R&D based on the needs attributes. In this study, we aimed to investigate the priority setting in R&D field, considering consumer's needs attributes. To be concrete, we tried to present the evolutional direction of desirable phased R&D according to 'the importance degree for consumers on the attributes (functions) of the certain products' and 'the urgency degree of technical quality to fulfill its needs'. To achieve this, we targeted SMART TV, the convergence product, which contains the uncertainty in terms of marketability and technological aspect, and analyzed the priority of the R&D in SMART TV field. Based on the result of the analysis, 4-steps product concept (ultra high definition TV, interactive TV, 3D/immersive TV, personalized TV) is derived by analyzing the evolutional direction of R&D in SMART TV field. This finding implies that the success possibilities of product could be enhanced during the process of the evolution of products that have multiple needs attributes, by pursuing the R&D which fulfills the needs attribute first required in the market. In addition, it provides a useful framework to design the R&D roadmap in an aspect of R&D strategy.

• Korean Journal of Child Education & Care
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• v.18 no.3
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• pp.105-121
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• 2018

Objective: The objective of this study was to explore the perceptions of pre-service teachers regarding their self-image as early childhood teachers, according to their grade. The subject of this study were 167 university students in early childhood department in Jeonnam province. Methods: In order to examine self-image as early childhood teachers, semantic differential meaning scale that was consist of 40 bipolar adjective items was administrated. Data were analysed by Multivariate Analysis of Variance (MANOVA). Results: First, pre-service teachers had positive self image as early childhood teacher. Second, according to grades the appreciation self image as early childhood teacher showed significant difference. To be more specific and are revealed significantly distinctness. But, score was not distinct by grade, and mean score of was lower than mean score of other area scores. Third, self image as early childhood teacher displayed the positively developing, that is to say, the image of early childhood teachers definitely changed by grade in teacher training course. Conclusion/Implications: The effect brought about this study indicate that the need of support socio-political concern for early childhood teachers and try to form positive teachers' image through organized in-service education.

• Journal of the Korean Association of Geographic Information Studies
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• v.8 no.4
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• pp.1-13
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• 2005

In the process of a hydrology circulation, evapotranspiration is considered a very important factor to build a plan for the development of water resources and to operate water resources system. This study purposes to estimate daily potential evapotranspiration quantity in consideration of energy factors of the surface by using spatial information such as Landsat TM (ETM+) data, DEM and Landcover. Kyounan-cheon, Han River is selected as a target area, and landcover is divided by vegetation and non-vegetation covered area. Penman-Monteith equation which considers leaf-area index is used to estimate potential evapotranspiration quantity of vegetation covered area. The combination method (energy burget and aerodynamic method) is used in non-vegetation covered area. Among the input data for estimating potential evapotranspiration, NDVI, SR and Albedo is formed by Landsat, TM and ETM+ from 1986 through 2002. ground heat flux is estimated by using NDVI distribution map, LAI distribution map is drawn by using SR distribution map. The result of estimation shows that the average potential evapotranspiration in the whole basin is about 1.8-3.2mm/day per each cell. THe results of estimating potential evapotranspiration quantity by each landcover are as follows; water surface 3.6-4.9mm/day, city 1.4-3.1mm/day, bareland 1.4-3.5mm/day, grassland 1.7-3.7mm/day, forest 1.7-3.0mm/day and farmland 1.8-3.6mm/day. The potential evapotranspiration quantity is underestimated in comparison with observed evaporation data by evaporation pan, but it is considered that it has physical propriety.

• The Journal of Dong Guk Oriental Medicine
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• v.7 no.2
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• pp.141-148
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• 1999

A simple and rapid FoLT(formamide low temperature)-PCR, whereby human genomic DNA from blood can be amplified without DNA preparative stps, is described using human insulin genes. By applicatin of FoLT-PCR in human insulin genes, intragenic polymorphism in non-coding regions of the human insulin gene was shown after amplification and analysis by restriction enzyme digestion. All nucleotide sequences were the same as the reported, and four necleotides, at 4 different positions were polymorphic, and polymorphic alleles ${\alpha}4$, ${\alpha}5$, ${\alpha}6$, and ${\beta}2$ were identified. The new alleles were originated from homologous recombination between the ${\alpha}1$ and ${\beta}1$ alleles, and the alleles were founded in heterozygotes only. Although allele ${\alpha}1$ was dominant, the new alleles and ${\beta}1$ were recessive. From the results, it was suggested that the new method of FoLT-PCR was highly applicable in genetic variation analysis.