• Development and Reproduction
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• v.5 no.2
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• pp.131-136
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• 2001

The developmental profiles of rpr, grim, dcp-1, diapl, diap2 transcripts, which were involved in programmed cell death, were analyzed using competitive RT-PCR in whole animals during Drosophila development. The fluctuation patterns of transcript levels of the apoptotic initiators(rpr and grim) were similar to those of the ecdysone titer in Drosophila life cycle. The transcript of dcp-1, which is considered as effector caspase, was expressed strong1y at early embryo and female adult stages. However, the transcript levels of anti-apoptotic factors diap1 and diap2, showed the reverse pattern comparing with those of apoptotic factors(rpr and grim). Also, the transcript levels of rpr, diap2 and dcp-1 were quantified in the salivary glands and wing discs dissected from the wandering late third instar larva. The transcript levels of rpr and diap2 were changed reversely each other in both tissues from wandering stage to puparium formation. These results suggest that the expressions of cell death related genes are regulated by the ecdysone signals during normal development.

• Tuberculosis and Respiratory Diseases
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• v.57 no.3
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• pp.250-256
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• 2004

Background : Bacterial infections in diabetic patients are an important cause of increased morbidity and mortality. It has been reported that bacterial infections in diabetics showed more impaired PMN functions such as reduced PMN respiratory burst and decreased microbicidal activity in inflammed tissues. Also, apoptosis(programmed cell death) is postulated to be a key mechanism for neutrophil elimination. It is very important that PMN apoptosis keeps the balance from an area of inflammation. Actuallly, as little was known about PMN apoptosis and respiratory burst in diabetes, we investigated PMN apoptosis and hydrogen peroxide production after endotoxin exposure. Methods : Peripheral venous blood samples were collected by routine venipuncture from healthy volunteers and diabetics to harvest neutrophils. We respectively measured the PMN apoptosis, the production of hydrogen peroxide, and the cell viability. Results : Normal neutrophils showed a tendency to decreased apoptosis after endotoxin treatment. In patients with diabetes, PMN apoptosis was significantly decreased compared with healthy controls. In addition, the LPS-induced neutrophils in diabetics demonstrated more decreased apoptosis. However, the production of hydrogen peroxide was not different between groups. Conclusion : These observations suggest that the decreased PMN apoptosis in diabetics with endotoxin exposure may also affect the increased susceptibility and severity of infections.

• Journal of Life Science
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• v.18 no.4
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• pp.461-466
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• 2008

Transforming growth $factor-{\beta}$ ($TGF-{\beta}$)-dependent apoptosis is important in the elimination of damaged or abnormal cells from normal tissues in vivo. Gadd45b has been known to participate in $TGF-{\beta}-induced$ apoptosis by the activation of p38 kinase. In this report, we show that Gadd45b is an immediate-early response gene for $TGF-{\beta}$ during apoptosis in EpH4 cells. To elucidate the molecular mechanism of $TGF-{\beta}-induced$ Gadd45b gene expression, we cloned the 5'-flanking region of the mouse Gadd45b gene. When transfected into EpH4 cells, this 5'-flanking region conferred promoter activity and inducibility by $TGF-{\beta}$. Deletion analyses demonstrated that the minimal promoter activity was detected in the proximal region 220 bp upstream of the transcription initiation site. We also found that the proximal Gadd45b promoter is activated by $TGF-{\beta}$ through the action of Smad2, Smad3, and Smad4. Finally, we show that the expression of Gadd45b gene by $TGF-{\beta}$ is suppressed in EpRas cells in which $TGF-{\beta}$ could not induce apoptosis, suggesting that Gadd45b may be a crucial target for $TGF-{\beta}-induced$ apoptosis in EpH4 cells.

• Journal of dental hygiene science
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• v.14 no.1
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• pp.1-6
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• 2014

The purpose of this study was to investigate the killing effect of Candida albicans on hairless mouse-2 (HRM-2) mouse tissues. We tested the effectiveness of a non-thermal atmospheric pressure plasma in killing C. albicans strains. The viability of C. albicans was determined by counting the colony forming units (CFU), after non-thermal atmospheric pressure plasma treatment. When non-thermal atmospheric pressure plasma was repeatedly treated on mouse skin which inoculated with C. albicans. The C. albicans cells were planted on skin tissue, and then the infected mouse tissue was exposed to non-thermal atmospheric pressure plasma for 0 sec, 60 sec, 180 sec and 300 sec. The death rate of C. albicans was increased in dependent with treatment times. The three times of non-thermal atmospheric pressure plasma at the interval of 10 minutes significantly showed the 6 log CFU/ml reduction of death rate on HRM-2 mouse tissues. Thus, non-thermal atmospheric pressure plasma could be used for the disinfection of C. albicans on oral surface.

• Journal of Life Science
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• v.22 no.11
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• pp.1500-1506
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• 2012

Transforming growth factor (TGF)-${\beta}$-dependent apoptosis is important in the elimination of damaged or abnormal cells from normal tissues, especially in liver, in vivo. To investigate which gene expressions are critical for TGF-${\beta}$-induced apoptosis in hepatocytes, gene expression profiling experiments were performed with TGF-${\beta}$-treated and non-treated mouse hepatocytes AML12 cells. Findings showed that serum and glucocorticoid-inducible protein kinase1 (SGK1) expression is markedly downregulated during TGF-${\beta}$-induced apoptosis. Findings confirmed that expression of SGK1 protein, as well as mRNA, is also markedly decreased with TGF-${\beta}$ treatment. Infection of adenoviral vector encoding constitutively active SGK1 (CA-SGK1), but not kinase dead SGK1 (KD-SGK1), attenuated TGF-${\beta}$-induced apoptosis. All of these results suggest that downregulation of SGK1 expression is critical for TGF-${\beta}$-induced apoptosis in AML12 cells.

• Development and Reproduction
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• v.15 no.2
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• pp.113-120
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• 2011

BCL-2 family essential proteins to play a pivotal role to perform in apoptosis signaling pathways and essential proteins for the regulation of cell death. BCL2L10 protein is a member of BCL-2 family and it regulates both anti-apoptotic and pro-apoptotic function of specific tissue or cell line. BCL2L10 of function and expression is not reported in ovary cell lines. In this study we reported that BCL2L10 were significant expression of KGN cell line. Ectopic expression of BCL2L10 induced cell death, and its cells killing effect was blocked by pan-caspase inhibitor of the Z-VAD-fmk. Ectopic expression of BCL2L10 protein led to the activation of caspase 9 and caspase 3, suggesting apoptotic cell death, and confocal microscopic analyses showed that BCL2L10 was partially localized in mitochondria. Thus, we provide a novel function of BCL2L10 in KGN cells, which was involved in the intrinsic cell death pathway.

• The Journal of the Korean bone and joint tumor society
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• v.11 no.2
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• pp.118-125
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• 2005

Purpose: The expression of apoptosis-related genes, such as survivin, bcl-2, and bax has been examined in the human osteosarcoma and then evaluated the correlation with clinical data of patients. Materials and Methods: Fifty human osteosarcoma specimens were established from incisional biopsy and examination of survivin, bcl-2, and bax by immunohistochemical study was performed. We investigated the correlation of survivin, bcl-2, bax and their two or three combined expressions with clinical data including the response of chemotherapy, local recurrence, distance metastasis, and oncologic outcome. Results: Survivin was showed in 26 cases (52%), bcl-2 in 23 cases (46%), and bax in 21 cases (42%) osteosarcoma. And coexpression of survivin and bcl-2 was showed in 19 cases (38%), survivin and bax in 13 cases (26%), bcl-2 and bax in 8 cases (16%), and all three expression was showed in 8 cases (16%). There was no correlation between their apoptosis related gene and histologic difference, the presence of local recurrence and distant metastasis. Whereas neoadjuvant chemotherapy response correlated with bcl-2 expression (P=0.04), and survivin and bcl-2 coexpression (P=0.044) with poor chemoresponse. The rate of died of disease was correlated with bcl-2 (P=0.001), survivin and bcl-2 coexpression (P=0.027) with bad outcome. Survival curves of bcl-2 (P=0.0075), survivin and bcl-2 (P=0.0012) was showed negative correlation in the Kaplan-Meier method. Conclusion: The apoptosis related gene expression was relatively high in osteosarcoma, bcl-2 expression was correlated with poor chemotherapy response and poor survival rate, but survivin was correlated with this oncologic outcome only in the bcl-2 coexpression. The examination of immunohistochemical stain of apoptosis related gene in osteosarcoma could be helpful in the judgment of osteosarcoma prognosis.

• Journal of Life Science
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• v.19 no.12
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• pp.1705-1711
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• 2009

Carcinoma cells that had acquired resistance to a chemotherapeutic drug often show cross-resistance to various other cytotoxic drugs. In the present study, we explored the effect of heat shock in cisplatin-resistant gastric cancer cells SNU601/Cis2 to figure out the efficacy of hyperthermia in drug-resistant carcinoma. While SNU601/WT cells showed a high-sensitivity response to heat shock by dying through apoptosis, SNU601/Cis2 cells were considerably resistant to mild heat shock, but died by necrosis upon treatment with harsh heat shock. The occurrence of necrosis in SNU601/Cis2 cells was linked to the suppression of both JNK1/2 activation and HSP27 induction in response to heat shock. Since necrosis is closely associated with tumor malignancy and poor prognosis through inflammatory responses, our result suggests that hyperthermic treatment should be carefully applied when it is combined with chemotherapy.

• Proceedings of the Korea Database Society Conference
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• 1999.06a
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• pp.415-421
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• 1999

Altman의 연구(1965, 1977)나 Beaver의 연구(1986)와 같은 전통적 예측모형은 분석자의 판단에 따른 예측도가 높은 재무비율을 선정하여 다변량판별분석(MDA： multiple discriminant analysis), 로지스틱회귀분석 등과 같은 통계기법을 주로 이용해 왔으나 1980년 후반부터 인공지능 기법인 귀납적 학습방법, 인공신경망모형, 유전모형 둥이 부실기업예측에 응용되기 시작했다. 최근 연구에서는 인공신경망을 활용한 변수 및 모형개발에 관한 보고가 있다. 그러나 지금까지의 연구가 주로 기업의 재무적 비율지표를 고려한 모형에 치중되었으며 정성적 자료인 비재무지표에 대한 검증과 선정이 자의적으로 이루어져온 경향이었다. 또한 너무 많은 입력변수를 사용할 경우 다중공선성 문제를 유발시킬 위험을 내포하고 있다. 본 연구에서는 부실기업예측모형을 수립하기 위하여 정량적 요인인 재무적 지표변수와 정성적요인인 비재무적 지표변수를 모두 고려하였다. 재무적 지표변수는 상관분석 및 요인분석들을 통하여 유의한 변수들을 도출하였으며 비재무적 지표변수는 조직생태학내에서의 조직군내 조직사멸과 관련된 생태적 과정에 대한 요인들 중 조직군 내적요인으로 조직의 연령, 조직의 규모, 조직의 산업밀도를 도출하여 4개의 실험집단으로 분류하여 비재무적 지표변수를 보완하였다. 인공신경망은 다층퍼셉트론(multi-layer perceptrons)과 역방향 학습(back-propagation )알고리듬으로 입력변수와 출력변수, 그리고 하나의 은닉층을 가지는 3층 퍼셉트론(three layer perceptron)을 사용하였으며 은닉충의 노드(node)수는 3개를 사용하였다. 입력변수로 안정성, 활동성, 수익성, 성장성을 나타내는 재무적 지표변수와 조직규모, 조직연령, 그 조직이 속한 산업의 밀도를 비재무적 지표변수로 산정하여 로지스틱회귀 분석과 인공신경망 기법으로 검증하였다. 로지스틱회귀분석 결과에서는 재무적 지표변수 모형의 전체적 예측적중률이 87.50%인 반면에 재무/비재무적 지표모형은 90.18%로서 비재무적 지표변수 사용에 대한 개선의 효과가 나타났다. 표본기업들을 훈련과 시험용으로 구분하여 분석한 결과는 전체적으로 재무/비재무적 지표를 고려한 인공신경망기법의 예측적중률이 높은 것으로 나타났다. 즉, 로지스틱회귀분석의 재무적 지표모형은 훈련, 시험용이 84.45%, 85.10%인 반면, 재무/비재무적 지표모형은 84.45%, 85.08%로서 거의 동일한 예측적중률을 가졌으나 인공신경망기법 분석에서는 재무적 지표모형이 92.23%, 85.10%인 반면, 재무/비재무적 지표모형에서는 91.12%, 88.06%로서 향상된 예측적 중률을 나타내었다.

• Proceedings of the Korea Inteligent Information System Society Conference
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• 1999.03a
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• pp.415-421
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• 1999

Altman의 연구(1965, 1977)나 Beaver의 연구(1986)와 같은 전통적 예측모형은 분석자의 판단에 따른 예측도가 높은 재무비율을 선정하여 다변량판별분석(MDA:multiple discriminant analysis), 로지스틱회귀분석 등과 같은 통계기법을 주로 이용해 왔으나 1980년 후반부터 인공지능 기법인 귀납적 학습방법, 인공신경망모형, 유전모형 등이 부실기업예측에 응용되기 시작했다. 최근 연구에서는 인공신경망을 활용한 변수 및 모형개발에 관한 보고가 있다. 그러나 지금까지의 연구가 주로 기업의 재무적 비율지표를 고려한 모형에 치중되었으며 정성적 자료인 비재무지표에 대한 검증과 선정이 자의적으로 이루어져온 경향이었다. 또한 너무 많은 입력변수를 사용할 경우 다중공선성 문제를 유발시킬 위험을 내포하고 있다. 본 연구에서는 부실기업예측모형을 수립하기 위하여 정량적 요인인 재무적 지표변수와 정성적 요인인 비재무적 지표변수를 모두 고려하였다. 재무적 지표변수는 상관분석 및 요인분석들을 통하여 유의한 변수들을 도출하였으며 비재무적 지표변수는 조직생태학내에서의 조직군내 조직사멸과 관련된 생태적 과정에 대한 요인들 중 조직군 내적요인으로 조직의 연령, 조직의 규모, 조직의 산업밀도를 도출하여 4개의 실험집단으로 분류하여 비재무적 지표변수를 보완하였다. 인공신경망은 다층퍼셉트론(multi-layer perceptrons)과 역방향 학습(back-propagation)알고리듬으로 입력변수와 출력변수, 그리고 하나의 은닉층을 가지는 3층 퍼셉트론(three layer perceptron)을 사용하였으며 은닉층의 노드(node)수는 3개를 사용하였다. 입력변수로 안정성, 활동성, 수익성, 성장성을 나타내는 재무적 지표변수와 조직규모, 조직연령, 그 조직이 속한 산업의 밀도를 비재무적 지표변수로 산정하여 로지스틱회귀 분석과 인공신경망 기법으로 검증하였다. 로지스틱회귀분석 결과에서는 재무적 지표변수 모형의 전체적 예측적중률이 87.50%인 반면에 재무/비재무적 지표모형은 90.18%로서 비재무적 지표변수 사용에 대한 개선의 효과가 나타났다. 표본기업들을 훈련과 시험용으로 구분하여 분석한 결과는 전체적으로 재무/비재무적 지표를 고려한 인공신경망기법의 예측적중률이 높은 것으로 나타났다. 즉, 로지스틱회귀 분석의 재무적 지표모형은 훈련, 시험용이 84.45%, 85.10%인 반면, 재무/비재무적 지표모형은 84.45%, 85.08%로서 거의 동일한 예측적중률을 가졌으나 인공신경망기법 분석에서는 재무적 지표모형이 92.23%, 85.10%인 반면, 재무/비재무적 지표모형에서는 91.12%, 88.06%로서 향상된 예측적중률을 나타내었다.