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BCL2L10 Protein Induces Apoptosis in KGN-Human Granulosa Cells  

Kim, Jae-Hong (Dept. of Pharmacy, College of Pharmacy, CHA University)
Lee, Kyung-Ah (Dept. of Biomedical Science, College of Biomedical Science, CHA University)
Bae, Jee-Hyeon (Dept. of Pharmacy, College of Pharmacy, CHA University)
Publication Information
Development and Reproduction / v.15, no.2, 2011 , pp. 113-120 More about this Journal
Abstract
BCL-2 family essential proteins to play a pivotal role to perform in apoptosis signaling pathways and essential proteins for the regulation of cell death. BCL2L10 protein is a member of BCL-2 family and it regulates both anti-apoptotic and pro-apoptotic function of specific tissue or cell line. BCL2L10 of function and expression is not reported in ovary cell lines. In this study we reported that BCL2L10 were significant expression of KGN cell line. Ectopic expression of BCL2L10 induced cell death, and its cells killing effect was blocked by pan-caspase inhibitor of the Z-VAD-fmk. Ectopic expression of BCL2L10 protein led to the activation of caspase 9 and caspase 3, suggesting apoptotic cell death, and confocal microscopic analyses showed that BCL2L10 was partially localized in mitochondria. Thus, we provide a novel function of BCL2L10 in KGN cells, which was involved in the intrinsic cell death pathway.
Keywords
Apoptosis; BCL2 family; BCL2L10; Granulosa tumor cell (KGN);
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1 Guillemin Y, Lalle P, Gillet G, François Guerin J, Hamamah S, Aouacheria A (2009) Oocytes and early embryos selectively express the survival factor BCL2L10. Mol Med 87:923-940.   DOI   ScienceOn
2 de Moraes-Ruehsen M, Jones GS (1967) Premature ovarian failure. Fertil Steril 18:440-461.   DOI
3 Arnoult D (2008) Apoptosis-associated mitochondrial outer membrane permeabilization assays. Methods 44:229- 234.   DOI   ScienceOn
4 Assou S, Anahory T, Pantesco V, Le Carrour T, Pellestor F, Klein B, Reyftmann L, Dechaud H, De Vos J, Hamamah S (2006) The human cumulus-oocyte complex gene-expression profile. Hum Reprod 21:1705-1719.   DOI   ScienceOn
5 Yoon SJ, Kim EY, Kim YS, Lee HS, Kim KH, Bae J, Lee KA (2009) Role of Bcl2-like 10 (Bcl2l10) in Regulating Mouse Oocyte Maturation. Biol Reprod 81:497-506.   DOI   ScienceOn
6 Zhang H, Holzgreve W, De Geyter C (2001) Bcl2-L-10, a novel anti-apoptotic member of the Bcl-2 family, blocks apoptosis in the mitochondria death pathway but not in the death receptor pathway. Hum Mol Genet 10:2329-2339.   DOI
7 Zou H, Li Y, Liu X, Wang X (1999) An APAF-1. cytochrome c multimeric complex is a functional apoptosome that activates procaspase-9. J Biol Chem 274:11549-11556.   DOI
8 Thompson CB (1995) Apoptosis in the pathogenesis and treatment of disease. Science 267:1456-1462.   DOI
9 Chipuk JE, Bouchier-Hayes L, Green DR (2006) Mitochondrial outer membrane permeabilization during apoptosis: the innocent bystander scenario. Cell Death Differ 13:1396-1402.   DOI   ScienceOn
10 Wang P, Yu J, Zhang L (2007) The nuclear function of p53 is required for PUMA-mediated apoptosis induced by DNA damage. Proc Natl Acad Sci USA 104:4054- 4059.   DOI   ScienceOn
11 Simmons MJ, Fan G, Zong WX, Degenhardt K, White E, Gelinas C (2008). Bfl-1/A1 functions, similar to Mcl-1, as a selective tBid and Bak antagonist. Oncogene 27:1421-1428.   DOI   ScienceOn
12 Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X (1997) Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 91:479-489.   DOI   ScienceOn
13 Song Q, Kuang Y, Dixit VM, Vincenz C (1999) Boo, a novel negative regulator of cell death, interacts with Apaf-1. EMBO J 18:167-178.   DOI
14 Nishi Y, Yanase T, Mu Y, Oba K, Ichino I, Saito M, Nomura M, Mukasa C, Okabe T, Goto K, Takayanagi R, Kashimura Y, Haji M, Nawata H (2001) Establishment and characterization of a steroidogenic human granulosalike tumor cell line, KGN, that expresses functional follicle-stimulating hormone receptor. Endocrinology 142:437-445.   DOI   ScienceOn
15 Reers M, Smiley ST, Mottola-Hartshorn C, Chen A, Lin M, Chen LB (1995) Mitochondrial membrane potential monitored by JC-1 dye. Methods Enzymol 260:406-417.
16 Shah SP, Kobel M, Senz J, Morin RD, Clarke BA, Wiegand KC, Leung G, Zayed A, Mehl E, Kalloger SE, Sun M, Giuliany R, Yorida E, Jones S, Varhol R, Swenerton KD, Miller D, Clement PB, Crane C, Madore J, Provencher D, Leung P, DeFazio A, Khattra J, Turashvili G, Zhao Y, Zeng T, Glover JN, Vanderhyden B, Zhao C, Parkinson CA, Jimenez-Linan M, Bowtell DD, Mes-Masson AM, Brenton JD, Aparicio SA, Boyd N, Hirst M, Gilks CB, Marra M, Huntsman D (2009) Mutation of FOXL2 in granulosa-cell tumors of the ovary. N Engl J Med 360:2719-2729.   DOI   ScienceOn
17 Kuwana T, Bouchier-Hayes L, Chipuk JE, Bonzon C, Sullivan BA, Green DR, Newmeyer DD (2005) BH3 domains of BH3-only proteins differentially regulate Bax-mediated mitochondrial membrane permeabilization both directly and indirectly. Mol Cell 17:525-535.   DOI   ScienceOn
18 Kim JH, Yoon S, Won M, Sim SH, Ko JJ, Han S, Lee KA, Lee K, Bae J (2009) HIP1R interacts with a member of Bcl-2 family, BCL2L10, and induces BAKdependent cell death. Cell Physiol Biochem 23:43-52.   DOI   ScienceOn
19 Melka M, Rings F, Holker M, Tholen E, Havlicek V, Besenfelder U, Schellander K, Tesfaye D (2010) Expression of apoptosis regulatory genes and incidence of apoptosis in different morphological quality groups of In Vitro-produced bovine pre-implantation embryos. Reprod Domest Anim 45(5):915-921.
20 Kim H, Rafiuddin-Shah M, Tu HC, Jeffers JR, Zambetti GP, Hsieh JJ, Cheng EH (2006) Hierarchical regulation of mitochondrion-dependent apoptosis by BCL-2 subfamilies. Nat Cell Biol 8: 1348-1358.   DOI   ScienceOn
21 Jurisicova A, Antenos M, Varmuza S, Tilly JL, Casper RF (2003) Expression of apoptosis-related genes during human preimplantation embryo development: potential roles for the Harakiri gene product and Caspase-3 in blastomere fragmentation. Mol Hum Reprod 9:133-141.   DOI
22 Hoek A, Schoemaker J, Drexhage HA (1997) Premature ovarian failure and ovarian autoimmunity. Endocr Rev 18:107-134.   DOI   ScienceOn
23 Kang Y, et al (2007) NM23-H2 involves in negative regulation of Diva and Bcl2L10 in apoptosis signaling. Biochem Biophys Res Commun 359:76-82.   DOI   ScienceOn
24 Ke N, Godzik A, Reed JC (2001) Bcl-B, a novel Bcl-2 family member that differentially binds and regulates Bax and Bak. J Biol Chem 276:12481-12484.   DOI
25 Hamamah S, Matha V, Berthenet C, Anahory T, Loup V, Dechaud H, Hedon B, Fernandez A, Lamb N (2006) Comparative protein expression profiling in human cumulus cells in relation to oocyte fertilization and ovarian stimulation protocol. Reprod Biomed Online 13:807-814.   DOI   ScienceOn
26 Hamatani T, Falco G, Carter MG, Akutsu H, Stagg CA, Sharov AA, Dudekula DB, VanBuren V, Ko MS (2004) Age-associated alteration of gene expression patterns in mouse oocytes. Hum Mol Genet 13:2263-2278.   DOI
27 Hartley PS, Bayne RA, Robinson LL, Fulton N, Anderson RA (2002) Developmental changes in expression of myeloid cell leukemia-1 in human germ cells during oogenesis and early folliculogenesis. J Clin Endocrinol Metab 87:3417-3427.   DOI   ScienceOn
28 Inohara N, Gourley TS, Carrio R, Muniz M, Merino J, Garcia I, Koseki T, Hu Y, Chen S, Nunez G (1998) Diva, a Bcl-2 homologue that binds directly to Apaf-1 and induces BH3-independent cell death. J Biol Chem 273:32479-32486.   DOI
29 Grondahl ML, Nielsen ME, Dal Canto MB, Fadini R, Rasmussen IA, Westergaard LG, Kristensen SG, Yding Andersen C (2007). Anti-Mullerian hormone remains highly expressed in human cumulus cells during the final stages of folliculogenesis. Reprod Biomed Online.