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http://dx.doi.org/10.5352/JLS.2012.22.11.1500

Downregulation of SGK1 Expression is Critical for TGF-β-induced Apoptosis in Mouse Hepatocytes Cells  

Nam, In-Koo (Department of Microbiology/Research Institute of Life Science, College of Natural Sciences, Gyeongsang National University)
Yoo, Jiyun (Department of Microbiology/Research Institute of Life Science, College of Natural Sciences, Gyeongsang National University)
Publication Information
Journal of Life Science / v.22, no.11, 2012 , pp. 1500-1506 More about this Journal
Abstract
Transforming growth factor (TGF)-${\beta}$-dependent apoptosis is important in the elimination of damaged or abnormal cells from normal tissues, especially in liver, in vivo. To investigate which gene expressions are critical for TGF-${\beta}$-induced apoptosis in hepatocytes, gene expression profiling experiments were performed with TGF-${\beta}$-treated and non-treated mouse hepatocytes AML12 cells. Findings showed that serum and glucocorticoid-inducible protein kinase1 (SGK1) expression is markedly downregulated during TGF-${\beta}$-induced apoptosis. Findings confirmed that expression of SGK1 protein, as well as mRNA, is also markedly decreased with TGF-${\beta}$ treatment. Infection of adenoviral vector encoding constitutively active SGK1 (CA-SGK1), but not kinase dead SGK1 (KD-SGK1), attenuated TGF-${\beta}$-induced apoptosis. All of these results suggest that downregulation of SGK1 expression is critical for TGF-${\beta}$-induced apoptosis in AML12 cells.
Keywords
Transforming growth factor-${\beta}$; Apoptosis; hepatocytes; serum and glucocorticoid-inducible protein kinase1 (SGK1); AML12 cells;
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