• Title/Summary/Keyword: 조직병리학

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Differentiation of Fusarium oxysporum f. sp. fragariae Isolates by Random amplified Polymorphic DNA (RAPD) Analysis. (Random Amplified Polymorphic DNA(RAPD)를 이용한 딸기 시들음병균(Fusarium oxysporum f. sp. fragariae)의 분류)

  • 현재욱;박원목
    • Korean Journal Plant Pathology
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    • v.12 no.1
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    • pp.41-46
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    • 1996
  • 본 실험은 이병 딸기의 조직에서 분리 동정된 시들음병균(Fusarium oxysporum f. sp. fragariae) 균주들의 유?거 변이를 random amplified polymorphic DNA(RAPD) marker들을 이용하여 조사하였다. 총 24개의 딸기 시들음병 균주들의 DNA를 주형으로 하여 16개의 random 10-mer primer들을 사용하여 증폭시킨 결과 총 231개의 marker들을 이용하여 유전적 변이를 조사해 본 결과 크게 RAPD I과 RAPD II의 2개 그룹으로 나눌 수 있었다. RAPD I그룹에 속하는 균주는 VCG A에 속하는 Y1, K1, K2, K3, K4, N2, N3, N4-1, N6-1, N6-2, N8, N9, N10, M1-2-1 균주, VCG B에 속하는 M4-1 균주 그리고 VCG C에 속하는 N1, Y2 균주들이었고, RAPD II그룹에는 VCG B에 속하는 M1-1, M2-2-1, M2-4-2, M3-2, M3-3-2 균주와 VCG D에 속하는 N1 1 균주가 속하였다. 이들 2그룹 간에는 31%의 유사성이 있었다.

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Occurrence of Bacterial Soft Rot of Melon Caused by Erwinia carotovora subsp. carotovora (Erwinia carotovora subsp. carotovora에 의한 메론의 세균성무름병 발생)

  • 이영근;김령희
    • Korean Journal Plant Pathology
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    • v.12 no.1
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    • pp.116-120
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    • 1996
  • Water-soaked spots or lesions were observed on fruits or stems of melon plants at house-melon farms in Andong, Korea. The symptoms developed to soft rot of the fruits of wilting of the whole plants. The causal organism isolated from the water-soaked le-sions was identified as Erwinia carotovora subsp. carotovora based on the morphological and physiological characteristics. The causal bacterium was susceptible to not only two kinds of medical antibiotics but also two kinds of agrochemicals tested. Since the bacterial soft rot is a first described bacterial disease in melon in Korea, we propose to name the disease as "bacterial soft rot of melon".

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An Effective Detection of Potato Virus Y Using RT-PCR Technique (RT-PCR 기법을 이용한 효과적인 감자바이러스 Y의 검정)

  • Joung, Young-Hee;Jeon, Jae-Heung;Choi, Kyung-Hwa;Kim, Hyun-Soon;Yi, Yong-Sub;Joung, Hyouk
    • Korean Journal Plant Pathology
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    • v.13 no.4
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    • pp.219-224
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    • 1997
  • A PT-PCR (reverse transcription-polymerase chain reaction) diagnostic method for potato virus Y (PVY) was developed using primer pair derived from conserved region of coat protein genes of several PVY strains, A 764 bp PCR product was detected from several lines of potato cv. Atlantic. We could prove that the 764 bp DNA fragment was indeed the PVY gene by sequencing analysis. PVY detection method using RT-PCR technique was about tuber tissue.

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Peroxidase Activity in Leaf Tissue of Rice Infected by Pyricularia oryzae (도열병에 감염된 벼의 엽조직에서 Peroxidase의 활성)

  • Park Won Mok;Lee Yong Se;Park Sang Ho
    • Korean Journal Plant Pathology
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    • v.1 no.3
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    • pp.178-183
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    • 1985
  • The present researches were carried out to investigate the peroxidase activity in association with the reactions of the 4 cultivars of rice plant, Nagdong, Jinheung, Nongbaek and Taebaek to Pyricularia oryzae race KJ-I0l and KJ-301. Although the peroxidase activity was increased during the growth of the rice seedlings, the significant difference in the activity was not found among 4 cultivars. After inoculation of the fungus, the peroxidase activity was enhanced in diseased leaves, being considerably higher in the compatible than in the incompatible cultivars. The isozyme bands of peroxidases observed in mycelium of rice blast fungus were not found in the diseased leaves on the gel electrophoresis. The peroxidase activity was not affected by the increased application of nitrogenous fertilizer.

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The Detection of Bovine Respiratory Syncytial Virus Using Immunohistochemistry and pathologic Findings (면역조직화학염색법을 이용한 소 합포체성 폐렴바이러스의 검출 및 병리학적 소견)

  • Yoon, Soon-Seek;Hwang, Eui-Kyung;Moon, Oun-Kyong;Bae, You-Chan;Kim, Jae-Hoon;Eom, Hyun-Jong;Cho, Jae-Jin;Jean, Young-Hwa;Choi, Sang-Ho
    • Korean Journal of Veterinary Pathology
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    • v.1 no.2
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    • pp.157-160
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    • 1997
  • Two 2 and 7 months-old holstin and one 3 months-old native Korean calf suffering from respiratory illness were submitted to the National Veterinary Research Institute for euthanasia and pathologic examination. At necropsy diffuse lobar pneumonia was present in apical cardiac and diaphragmatic lobes in all calves. Microscopically acute multifocal bronchiolitis and interstitial syncytial cell formation were frequently observed. in addition occlusion of bronchiolar lumen due to cellular proliferation and fibrosis accompanied by interlobular septal emphysema were also present. Immunohistochemically bovine respiratory syncytial virus antigen were positively identified in lung lesions including bronchial and bronchiolar epithelium alveolar macrophages and lymphocytes.

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Fine Needle Aspiration Cytology of Carotid Body Paraganglioma - A Case Report - (부신경절종의 세침 흡인 세포학적 소견 - 1례 보고 -)

  • Kim, Joon-Mee;Chu, Young-Chae
    • The Korean Journal of Cytopathology
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    • v.4 no.1
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    • pp.77-80
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    • 1993
  • Paraganglioma is a benign tumor arising in the paraganglion system scattered throughout the body, but its cytopathologic findings arenot well known. We experienced a case of paraganglioma of carotid body diagnosed by fine needle aspiration. The patient was a 30 year-old female who suffered from the left neck mass for 3 years. The mass was $3\times3cm$ in size without pulsation or bruit. Cytologically, the smear revealed aggregated and singly scattered tumor cells haying abundant pale cytoplasm and indistinct cell borders. Their nuclei were round to oval, but enlarged nuclei were occasionally observed. The nuclear membrane was smooth with fine clumping of chromatin. Differentiation from metastatic follicular carcinoma of the thyroid gland was difficult.

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Histological Analysis Effect of 'Sexiang Shuhuo Jing' for after Skeletal Muscle in Rats (골격근 손상에 대한 '사향서활정(麝香舒活精)' 치료 효과에 대한 조직형태학적 관찰)

  • Kim, Jin-Hang;Song, Je-Ho
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.20 no.6
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    • pp.1543-1547
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    • 2006
  • The aim of this experiment was to observe and analysis clinical effect of the 'Sexiang shuhuo Jing' on histological change for 14days after skeletal muscle injury in rats. The gastrocnemius muscles of rats were damaged by electromechanical and serial cryosections of the damaged muscle were prepared at 1, 5, 10, 14 days after injury. Muscle sample of the both control and 'Sexiang Shuhuo Jing' treated group were prepared for histological analysis by optical microscope and electron microscopy. 'Sexiang Shuhuo Jing' treatment group's skeletal muscle recovery was much more faster than control group. After 5 day's 'Sexiang Shuhuo Jing' treatment group's basically recovery normal structure of muscle fiber. After 14 day's control group's damaged muscle were basically recovery structure of muscle fiber but still has some factor of pathological impression but in Sexiang Shuhuo Jing treatment group's can't be found that.

Clinical Study on the Relations of the Refineness and the Tactile of Back Skin of the Hand to Sasang Constitutions depending on sex and age (연령 및 성별에 따른 사상체질별 손등 피부의 조직 세밀도 및 감촉 특성에 대한 임상 연구)

  • Lee, Su-Heon;Joo, Jong-Cheon;Yoon, Yoo-Sik;Kim, Jong-Yeol
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.19 no.2
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    • pp.536-543
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    • 2005
  • We statistically analyzed the relationship between the constitution and the refineness and tactile of skin depending on sex and age, using 1079 clinical data registered to SCIB(Sasang constitution Information Bank), and the following results are obtained: The thickness of skin has big discrimination ability in classification of Taeeumin and Soyangin, especially in women and in ages 21 or more. The stiffness of skin also has big discrimination ability in classification of Taeeumin and Soeumin, especially in Taeumin women and Soeumin man and in ages 21-60. The differences stated above have been proved to be meaningful enough by Chi-square test.

Effects of Platycodi Radix on Passive Smoking in Rats (간접흡연에 노출시킨 흰쥐의 폐조직 손상에 대한 길경의 보호효과)

  • Kim, Bum-Hoi
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.26 no.1
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    • pp.40-46
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    • 2012
  • Cigarette smoking is known to be associated with various chronic pulmonary and cardiovascular diseases ranging from inflammation to cancer. Not only first-hand smoke but also second-hand smoke is suggested to be a factor of health risk. This study was to investigate whether Platycodi Radix extract administration would alter oxidative stress in lung leading to protection of cigarette smoke-induced lung damage. Sprague-Dawley rats were randomly divided into 3 groups; Intact, Smoke+PR and Smoke+Vehicle. In Smoke+PR and Smoke+Vehicle group, the exposure to cigarette smoke was performed for 15 min/day for 4 weeks in ventilated smoking chamber. The Platycodi Radix extract and saline were orally administrated to Smoke+PR and Smoke+Vehicle group each. The rats of Intact group were just kept in ventilated chamber without cigarette smoke. After the experiment for 4 weeks, the lung tissues were collected for histological observation and immunohistochemistry. In Results, airspace enlargement and goblet cell hyperplasia were observed after 4 weeks' exposure to cigarette smoke. Whereas, the oral administration of Platycodi Radix extract for 4 weeks reduced airspace enlargement and goblet cell hyperplasia. Moreover, the alterations of BAX/Bcl-2 proteins in lung tissues were observed. These results suggest that Platycodi Radix extract ameliorates lung damage in cigarette smoke-exposed rats and has protective effects on second-hand smoke injury.

Primary Synovial Chondromatosis Presented as Non-Calcified Loose Bodies in the Popliteal Cyst (슬와 낭종 내부의 비석회화성 유리체로 발현된 원발성 활액막성 연골종증)

  • Cha, Jae-Ryong;Suh, Jae-Hee;Chang, Sung-Who;Park, Ki-Bong
    • Journal of the Korean Orthopaedic Association
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    • v.56 no.4
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    • pp.346-350
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    • 2021
  • A magnetic resonance imaging test was performed on a 56-year-old male patient, who visited with a one-month history of painless swelling at the popliteal area of the left knee. A popliteal cyst and non-calcified loose body in the cyst were identified. Synovial chondromatosis was diagnosed from a histology examination by excision and biopsy. This paper reports this case of extra-articular synovial chondromatosis of the knee with a review of the relevant literature.