• Applied Biological Chemistry
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• v.36 no.3
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• pp.139-145
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• 1993

The methods of isoelectric focusing of 4 isozymes in polyacrylamide horizontal slab gels and restriction fragment length polymorphisms (RFLPs) were applied to characterize the biochemical phenotypes of 19 cultivars of barley. Among 19 barley cultivars screened, 7 esterase, 3 phosphoglucose isomerase, 4 peroxidase and 2 alcohol dehydrogenase isozyme phenotypes were distinguished by isoelectric focusing. When purified DNA of each cultivar was digested with restriction enzyme EcoRV and analyzed its RFLPs with barley DNA markers pMSU 51 or pMSU 71, two distinct RFLP patterns were shown. Based on the four isozymes and two RELP polymorphisms, 19 cultivars of barley were classified into 13 biochemical phenotypes. Phylogenetical relationships among 13 biochemical phenotypes classified were determined using Nei's F-statistics and the phylogenetic dendrogram was constructed.

• Applied Biological Chemistry
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• v.42 no.1
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• pp.34-38
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• 1999

We observed the structure of phenylalanine ammonia-lyase gene (PAL) which is one of the best studied plant defense-related genes responding to pathogen infection by producing suberin, lignin, and phytoalexins. In tomato, at least 5 different genetic loci have been identified by genomic southern blot hybridization and nucleotide sequence analyses of partially cloned gene fragments (Lee et al. 1992). However, our results suggest that two other isoforms designated as PAL X1 and PAL X2 are located on the chromosome in tomato plant. Furthermore, the preliminary results obtained from southern blot hybridization analyses of subcloned fragment digested with several restriction endonuclease indicated that PAL X1 and PAL X2 clones contain at least two copies of PAL gene and partial nucleotide sequence analyses of each subcloned fragment with the same primer taken from known nucleotide sequence of PAL5 gene indicated that they are located side by side on the same chromosome.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1986.12a
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• pp.518.2-519
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• 1986

토양으로부터 분리한 질소고정균인 Klebsiella pneumoniae NFB320의 chromosomal DNA를 BamHI으로 절단하여 동일한 제한효소로 절단한 pBR322에 ligation시켜 E. coli HB101에 형질전환을 행하여 pullulanase activity를 나타내는 clone을 얻어내었다. 이 형질 전환체로부터 분리한 pullulanase 유전자가 재조합된 plasmid DNA는 약 10kb의 DNA단편을 가지고 있었으며, 재조합된 plasmid로부터 생산되는 pullulanase의 특성은 최적 활성 pH가 6.0이며, 효소의 pH안정성은 5-10이었다. 또한 형질 전환체로부터 생산되는 pullulanase의 localization,효소활성에 영향을 미치는 온도안정성 둥을 조사하였다.

• 한국디지털정책학회:학술대회논문집
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• 2005.11a
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• pp.27-43
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• 2005

정보시스템 감리 시 시간적인 제한과 제한적인 검토로 인하여 문제점 도출에 한계가 있다. 이로 인하여 정보화계획 수립, 분석, 설계, 구현단계 산출물과 시스템에 대한 지적사항이 단편적으로 된다. 본 연구에서는, 정보시스템 감리 보고서에서 지적되었던 프로젝트관리에 대한 검토사항 및 지적사항을 유형별로 분석 하여, 체크리스트를 개선하였고, 이를 적용하여 프로젝트 관리를 효과적으로 하도록 제안하였다. 개선된 체크리스트를 이용하여 정보화 사업관리자가 관리의 포인트로 활용할 수 있고, 감리 시 생산성을 높이며 정보시스템 감리의 품질이 향상 될 것이다. 본 연구에서 제안한 개선된 체크리스트는 향후 자료를 보완하고 유형별 사례를 보완하면 프로젝트 관리 성과 측정 툴로서도 활용될 수 있을 것으로 기대한다.

• Journal of KIISE:Computer Systems and Theory
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• v.33 no.9
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• pp.626-633
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• 2006

The wireless sensor networks are sensing, computing and communication infrastructures that allow us to monitor, instrument, observe, and respond to phenomena in the harsh environment. Sensor operating systems that run on tiny sensor nodes are the key to the performance of the distributed computing environment for the wireless sensor networks. Therefore, sensor operating systems should be able to operate efficiently in terms of energy consumption and resource management. In this paper, we present an efficient memory allocation scheme to improve the time and space efficiency of memory management for the sensor operating systems. Our experimental results show that the proposed scheme performs efficiently in both time and space compared with existing memory allocation mechanisms.

• Korean Journal of Microbiology
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• v.39 no.3
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• pp.141-147
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• 2003

The simultaneous expression levels of antifungal activity related genes was analyzed by DNA microarray. We constructed DNA chips contained 2,000 randomly digested genome spots of the antifungal bacterium of Bacillus lentimorbus WJ5 and compared its quantitative aspect with 7 antifungal activity deficient mutants induced by gamma radiation ($^{60}Co$). From the analysis of microarray hybridization by the Gene Cluster (Michael Eisen, Stanford Univ.), totally 408 genes were expressed and 20 genes among them were significantly suppressed in mutants. pbuX (xanthine permease, K222), ywbA (phosphotransferase system enzyme II, K393), ptsG (PTS glucose specific enzyme II ABC component, K877), yufO (ABC transporter (ATP-binding protein), K130l), and ftsY (signal recognition particle (docking protein), K868) were simultaneously down-regulated in all mutants. It suggested that they were supposed to be related to the antifungal activity of B. lentimorbus WJ5.

• The Journal of Natural Sciences
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• v.5 no.1
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• pp.37-45
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• 1992

The gene coding for $\beta$-galactosidase of Neisseria lactamica 2118 was cloned into Escherichia coli MC 1061. The isolated 6.5 Kb EcoR I fragement and 7.2 Kb BamH I fragment of chromosomal DNA in Southern hybridization were ligated to a vector plasmid pBR322 and then transformed into Escherichia coli MC 1061 cells. Finally, I obtained three clones as $\beta$-galactosidase positive clone by colony hybridization and Southern hybridization($\beta$-galactosidase probe: lac Z gene of pMC1871). Three recombinant plasmids(pNL.13. 17 and 24) were found to contain the 7.2Kb BamH I fragment originated from Neisseria lactamica 2118 chromosomal DNA by Southern hybridization and pNL 24 was showed high homology to probe especially and also its physical map was constructed.

• Journal of KIISE:Databases
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• v.28 no.3
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• pp.315-323
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• 2001

The concept of vertical partitioning has been discussed so far in an objective of improving the performance of query execution and system throughput. It can be applied to the areas where the match between data and queries affects performance, which includes partitioning of individual files in centralized environments, data distribution in distributed databases, dividing data among different levels of memory hierarchies, and so on. In general, a vertical partitioning algorithm should support n-ary partitioning as well as a globally optimal solution for the generation of all meaningful fragments. Most previous methods, however, have some limitations to support both of them efficiently. Because the vertical partitioning problem basically includes the fuzziness property, the proper management is required for the fuzziness problem. In this paper we propose an efficient vertical $\alpha$-partitioning algorithm which is based on the fuzzy theory. The method can not only generate all meaningful fragments but also support n-ary partitioning without any complex mathematical computations.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.29 no.4
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• pp.835-845
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• 2019

File Carving is a technique for attempting to recover a file without metadata, such as a formated storage media or a damaged file system, and generally looks for a specific header / footer signature and data structure of the file. However, file carving is faced with the problem of recovering fragmented files for a long time, and it is very important to propose a solution for digital forensics because important files are relatively fragmented. To overcome these limitations, various carving techniques and tools are continuously being developed, and data sets from various researches and institutions are provided for functional verification. However, existing data sets are ineffective in verifying tools because of their limited environmental conditions. Therefore, this paper refers to the importance of fragmented file carving and develops 16 images for carving tool verification based on scenarios. The developed images' carving rate and accuracy of each media is shown through Foremost which is well known as a commercial carving tool.

• Korean Journal of Microbiology
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• v.40 no.2
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• pp.172-177
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• 2004

Photosynthetic bacteria, Rhodospirillum rubrum, have been reported to change their metabolic patterns depend-ing on the light condition. The genetic approach for such a metabolic change is one of main subject in pho-tosynthetic bacteria. It has been reported that the extrachromosomal plasmid might be related to this metabolic regulation. In this study, we have determined the partial sequences of R. rubrum plasmid pKYl with HindIII fragments and the predicted pKYl ORFs and physical map. We found the 8 putative proteins related to the genetic recombination of bacterium, which is reported to the alternative gene expression. Our results suggest that the genes located in pKYl are possibly involved in the metabolic switch according to the photocondition.