• Journal of the Korean Society for Library and Information Science
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• v.57 no.2
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• pp.123-150
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• 2023

This study aimed to propose the core values of librarians by investigating the core values of domestic and foreign libraries, including ALA's core values, and analyzing related research by domestic and foreign researchers. The research process included literature analysis, case studies, and surveys. The results showed that although the recognition of the need for setting core values for librarians was high (4.48), the awareness and understanding of core values were low. The reasons for recognizing the need for setting core values included improving the recognition and value of librarians, enhancing their professionalism, and establishing the value of libraries. The study proposed approximately 20 core values for librarians, with only two items scoring below 4 points, and 18 items showing high agreement with scores of 4 points or above. Particularly, professionalism, accessibility, diversity, service, literacy, and public interest all scored above 4.4. Analysis of perception differences between different types of libraries revealed significant differences for almost all items, with school libraries showing the most significant differences. The study recommends conducting in-depth research at the national level to reduce the number of core values to within 10 and share them with the nation.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2002.10a
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• pp.159-159
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• 2002

양서류를 이용한 내분비계장애물질의 환경독성시험기법을 확립하기 위해 Xenopus laevis의 변태 과정에 thyroid계 물질을 처리하여 이들의 효과에 의한 내분비계 장애물질의 판정 기법을 확립하고자 하였다. 올챙이의 발달 단계에 의한 내분비계 장애물질에 대한 민감도를 고려하여, embryo 단계에서 tail resorption 과정의 전단계인 stage 11로부터 30일간(방법1), stage 49로부터 28일간(방법2)와 tail resorption 과정 및 이후 stage 57로부터 17 내지 21일간(방법3)으로 구분하여 시험물질을 노출시켰으며 대조군과 비교하여, 시험물질에 노출된 올챙이의 기형적 변태 정도는 developmental stage 변화 속도, head-tail length 차이, 및 body weight 변화 등을 endpoints로 하였다.

• Journal of Energy Engineering
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• v.4 no.3
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• pp.315-323
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• 1995

차세대의 새로운 발전방식인 인산형 연료전지 발전시스템의 운용기술의 확립을 위하여 50 kW급 실증설비를 도입하고 주변기기를 설계, 설치하여 장기운전 특성시험을 실시하였다. 이 설비는 1993년 8월부터 1994년 11월 말까지 총 6,003시간의 운전기간 동안 187,190 kWh의 발전전력량을 기록하였고, 이 기간 동안의 발전운전시간 이용율은 63.01%, 평균출력은 33.5 kW였다. 설비의 운전특성에 있어서 정부하시 평균전압 및 전류는 각각 DC 130 V와 461 A였으며, 경시전압감소율은 1,000시간당 3.8 mV 정도로 나타났다. 설비의 기동·정지 특성중 기동시간은 냉간기동시 평균 4시간 50분, 난기동시 2시간 34분 정도가 소요되었고, 발전효율은 정부하시 전기효율이 37.46%, 배열이용효율은 43.8%로 전체효율 81.26%를 나타냈다. 환경 특성중 배가스중의 NOx 농도는 1 ppm 미만이었고, 소음도 63 dB 정도로 양호한 특성을 보여주었다. 운전기간 중 발생한 사고는 총23회로, 외부운전조건에 대한 민감성이 큰 것으로 나타났으며, PAFC 시스템의 상용화를 위해서는 스택수명 및 운전신뢰성을 향상시키는 것이 필요하다.

• Research in Plant Disease
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• v.17 no.2
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• pp.161-168
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• 2011

To establish simple and reliable screening method for resistant radish to Plasmodiophora brassicae Woron. using soil-drenching inoculation, the development of clubroot on radish seedlings inoculated with P. brassicae GN-1 isolate according to several conditions such as inoculum concentration, plant growth stage and incubation period after inoculation was studied. To select resistant radish against clubroot, 10-day-old seedlings were inoculated with P. brassicae by drenching the roots with the spore suspension of the pathogen to give $1{\times}10^9$ spores/pot. The inoculated seedlings were incubated in a growth chamber at $20^{\circ}C$ for 3 days then cultivated in a greenhouse ($20{\pm}5^{\circ}C$) for 6 weeks. Under the optimum conditions, 46 commercial cultivars of radish were tested for resistance to YC-1 (infecting 15 clubroot-resistant cultivars of Chinese cabbage) and GN-1 (wild type) isolates of P. brassicae. Among them, thirty-five cultivars showed resistance to both isolates and one cultivar represented susceptible response to the pathogens. On the other hand, the other cultivars showed different responses against the tested P. brassicae pathogens. The results suggest that this method is an efficient system for screening radish with resistance to clubroot.

• Journal of Life Science
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• v.25 no.2
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• pp.158-163
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• 2015

Rice embryo is more abundant than endosperms in nutrients such as proteins, lipids, and vitamin B1. In this study, we constructed p53 plasmid that could be expressed in a plant system, and investigated optimal germination conditions in a variety of media. For construction of p53 plasmid, we performed p53 amplification from pCDNA-p53, subcloned to TA cloning vector, and then reconstructed into pGEM-CaMV plant expression vector. On the other hand, we prepared a variety of imbibition buffers and complete media for efficient germination of the rice embryo. Imbibition buffers prepared with different concentrations of salt or detergent showed no significant effect on germination efficiency. We prepared further culture media, such as solid agar, liquid media, and paper towel to establish the optimal conditions. Rice embryo showed germination rates of more than 70% in the solid medium, more than 60% in the paper towel medium, but less than 25% in liquid media, although germination rate did not differ with varying concentrations of salt and sucrose in culture media. Under the optimal germination conditions, we introduced the p53 plasmid using imbibition method, and finally detected human p53 gene expression in the germinated rice embryo. This method might present a novel, practical approach for evaluating efficient gene expression utilizing imbibition method in rice embryo.

• Journal of Korea Entertainment Industry Association
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• v.14 no.8
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• pp.265-276
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• 2020

The purpose of this study was to investigate major satisfaction, communication skills, image of nurses and nursing professionalism, and to identify the influencing factors on nursing professionalism of nursing students. Data were collected using questionnaires from 214 students who were 3rd years in the nursing university in Gwangju, June 2019. The collected data was analyzed by descriptive statistics, pearson's correlation coefficient and stepwise multiple regression using IBM SPSS 21.0 program. The mean scores for the major satisfaction(3.71±0.53), communication skills(3.50±0.38), image of nurses(3.80±0.52), nursing professionalism(3.70±0.56) were above average. It was found that there were significant differences in the nursing professionalism depending on age, motivation for nursing clinical practice. Significant relationships were found among major satisfaction(r=.531, p=.000), communication skills(r=.531, p=.000), image of nurses(r=.836, p=.000) and nursing professionalism in nursing students. The results of stepwise multiple regression indicates that image of nurses(β=.705, p=.000) and communication skill(β=.136, p=.001) and major satisfaction(β=.133, p=.002) and predicts 72.8% in clinical practice competency(F=191.248, p<.001). In conclusion, to enhance nursing professionalism for nursing students, it is necessary to develop and utilize a program that enhances image of nurses, communication skills and major satisfaction.

• Development and Reproduction
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• v.3 no.1
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• pp.45-51
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• 1999

It is crucial to remove trophectoderm (TE) cells of blastocysts for an efficient isolation of pluripotent embryonic stem (ES)-like cells from bovine blastocysts. We evaluated the effectiveness of chemosurgery using calcium ionophore A23l87 (CIPA) by investigating the viability and pluripotency of ES-like cell lines isolated from in vitro-produced bovine blastocysts after CIPA treatment. The blastocysts treated with 50 $\mu$M CIPA for 25 min colonized most efficiently (51% of blastocysts) and developed to ES-like cell lines through 10 passages (4.8% of blastocysts) among CIPA-treated groups with different concentration and duration. In comparison with CIPA-untreated blastocysts, the colonization rate and overall viability of the CIPA-treated blastocysts were five times higher, suggesting that CIPA treatment condition defined in this study was highly efficient for establishing ES-like cell lines without apparent toxicity of CIPA. We evaluated in vitro pluripotency of the established three ES-like cell lines by examining alkaline phosphatase (AP) activity, capability of embryoid body formation, and chromosomal euploidity of the cells. Our cells showed a heterogeneous AP activity similarly to other reports. The cells were able to form simple embryoid bodies during suspension culture and majority of them showed a normal chromosome number of 60, the euploid chromosomal complement of bovine Therefore, our data suggest that CIPA treatment can be safely used for an efficient isolation of ES-like cell lines from bovine blastocysts.

• Korean Journal of Food Science and Technology
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• v.38 no.2
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• pp.176-182
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• 2006

Sensitive and specific monoclonal antibody (MAb) was produced from hybridoma (1H11-5) obtained by fusion of myeloma cell (V653) and spleen cell isolated from mouse immunized sulfamthazine (SMZ)-HG-KLH. Direct competitive ELISA was developed for rapid detection of SMZ in milk samples using MAb against SMZ with optimized conditions between MAb and SMZ-HG-HRP conjugate, and applicable conditions for analysis of milk samples were established. Detection range of immunoassay was 0.1 to 100 ppb. Recoveries from spiked raw milk and processed milk samples averaged 82.1-120.7 and 82.1-97.1%, respectively.

• ETRI Journal
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• v.10 no.1
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• pp.64-75
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• 1988

GaAs 등 화합물 반도체는 그 표면구조가 아직 확립되어 있지 않고, 표면조건이 소자특성에 큰 영향을 미친다. 소자공정중 이온주입 공정은 self-aligned MESFET(Metal Semiconductor Field Effect Transistor) 제작에 필수적인 기술이나, 이온 주입시 수 $\AA$ 크기의 vacancy 등 격자결함이 발생하며 이들 결함을 제어할 수 있는 기술이 필요하다. 에너지 가변 양전자 소멸기술은 표면에서 $1\mum$정도내에 존재하는 vacancy 형태의 격자결함을 감지해 낼 수 있으며 이들 격자결함의 depth profiling을 할 수 있는 기술이다. 본 고에서는 에너지 가변 양전자 소멸기술의 원리 및 최근 연구결과에 대해서 살펴보기로 한다.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2003.05a
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• pp.122-123
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• 2003

물리적인 건식 방법으로 소각 및 매립되는 생활계 폐플라스틱에 포함된 이물질을 제거하는 공정을 확립 연구를 수행하였다. 수집 운반되는 생활계 폐플라스틱 시료의 발생 장소, 위치 및 시간에 따라 포함된 이물질 종류가 다르고 수분의 함량에 있어 차이가 많이 있어 투입되는 시료에 대한 일차 이물질 분석이 필요하다. 이물질 분리 공정은 운반 $\rightarrow$ 파쇄 $\rightarrow$ 선별 분리(자력, 와류, 스크린) $\rightarrow$ 저장의 순서로 진행하였으며 파쇄시 흙 종류가 많이 포함된 경우에는 -0.8cm로 파쇄할 경우 효과적이나 일반적으로 -1.2cm 정도로 파쇄하여 이물질을 선별 분리하여도 이물질이 함유량이 3%이하이며, 회수율은 97% 이상이었다.