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Development of Convenient Screening Method for Resistant Radish to Plasmodiophora brassicae

효율적인 무 뿌리혹병 저항성 검정법 확립

  • Jo, Su-Jung (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Jang, Kyoung-Soo (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Choi, Yong-Ho (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Kim, Jin-Cheol (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Choi, Gyung-Ja (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology)
  • 조수정 (한국화학연구원 산업바이오화학연구센터) ;
  • 장경수 (한국화학연구원 산업바이오화학연구센터) ;
  • 최용호 (한국화학연구원 산업바이오화학연구센터) ;
  • 김진철 (한국화학연구원 산업바이오화학연구센터) ;
  • 최경자 (한국화학연구원 산업바이오화학연구센터)
  • Received : 2011.07.12
  • Accepted : 2011.08.17
  • Published : 2011.08.31

Abstract

To establish simple and reliable screening method for resistant radish to Plasmodiophora brassicae Woron. using soil-drenching inoculation, the development of clubroot on radish seedlings inoculated with P. brassicae GN-1 isolate according to several conditions such as inoculum concentration, plant growth stage and incubation period after inoculation was studied. To select resistant radish against clubroot, 10-day-old seedlings were inoculated with P. brassicae by drenching the roots with the spore suspension of the pathogen to give $1{\times}10^9$ spores/pot. The inoculated seedlings were incubated in a growth chamber at $20^{\circ}C$ for 3 days then cultivated in a greenhouse ($20{\pm}5^{\circ}C$) for 6 weeks. Under the optimum conditions, 46 commercial cultivars of radish were tested for resistance to YC-1 (infecting 15 clubroot-resistant cultivars of Chinese cabbage) and GN-1 (wild type) isolates of P. brassicae. Among them, thirty-five cultivars showed resistance to both isolates and one cultivar represented susceptible response to the pathogens. On the other hand, the other cultivars showed different responses against the tested P. brassicae pathogens. The results suggest that this method is an efficient system for screening radish with resistance to clubroot.

Plasmodiophora brassicae Woron.에 의해 발생하는 무 뿌리혹병에 대한 효율적인 저항성 검정법을 확립하기 위하여, GN-1 균주를 사용하여 접종원 농도, 무 생육 시기 및 접종 후 배양 기간 등 발병 조건에 따른 무 뿌리혹병발생을 조사하였다. 종자를 파종하고 10일 동안 재배한 무 유묘에 뿌리혹병균을 포트 당 $1.0{\times}10^9$개의 포자 농도가 되도록 관주하여 접종하였을 때 뿌리혹병이 가장 많이 발생하였다. 그리고 뿌리혹병 발생을 위해서, 접종한 무 유묘는 $20^{\circ}C$ 생육상에서 하루에 12시간 동안 광을 처리하며 3일 동안 배양하고, 그 후 온실($20{\pm}5^{\circ}C$)에서 6주 동안 재배한 후에 발병 정도를 조사하는 것이 효율적이었다. 확립한 무 뿌리혹병 저항성 검정법을 이용하여, 시판 중인 무 품종 46개의 P. brassicae GN-1 균주(저항성 배추 품종을 침입할 수 없는 균주)와 YC-1 균주(15개 저항성 배추 품종에 뿌리혹병을 일으키는 균주)에 대한 저항성 정도를 실험한 결과, 실험한 무 품종 중 35개 품종은 두 균주 모두에 대하여 저항성을, 그리고 1개 품종은 감수성을 나타내었다. 한편, 나머지 10개 품종은 균주에 따라 서로 다른 반응을 나타냈다. 이상의 결과로부터 본 연구에서 확립한 뿌리혹병 저항성 검정법은 뿌리혹병균에 대한 무의 저항성을 조사하기 위한 효율적인 방법임을 알 수 있었다.

Keywords

References

  1. 구경형, 이경아, 김영림, 이명기. 2006. 전처리 방법이 무청의 표면 미생물 변화에 미치는 영향. 한국식물영양과학회지 35: 511-672. https://doi.org/10.3746/jkfn.2006.35.5.649
  2. 이희정, 이유정, 양덕조. 2008. 새로운 세포질 웅성불임 계통 무 (Raphanus sativus L.)의 미토콘드리아 DNA 유전적 특성. 자연과학연구 22: 107-118.
  3. 장세정, 허승환, 장창순, 간성우, 임용표, 김홍기. 2007. 국내 배추 뿌리혹병균, Plasmodiophara brassicae의 race와 그 우점 양상. 식물병연구 13: 45-49.
  4. 조광수, 한영환, 이정태, 허은주, 양태진, 우종규. 2002. 고랭지 지역 배추 무사마귀병원균의 생리형 분화와 저항성 품종 선발. 한국육종학회지 34: 168-173.
  5. 조수정, 장경수, 최용호, 김진철, 최경자. 2010. 관주 접종법을 이용한 효율적인 배추 뿌리혹병 저항성 검정법. 식물병연구 16: 279-284. https://doi.org/10.5423/RPD.2010.16.3.279
  6. 조영환. 2008. 내병성 무 품종 육성: 무. 한국채소종자산업발달사, 서울대학교출판부, pp. 271-280.
  7. Akaba, M., Kaneko, Y., Hatakeyama, K., Ishida M., Bang, S. W. and Matsuzawa, Y. 2009. Identification and evaluation of clubroot resistance of radish chromosome using a Brassica napus-Raphanus sativus monosomic addition line. Breeding Science 59: 203-206. https://doi.org/10.1270/jsbbs.59.203
  8. Akito, K., Masato, T., Nakao, K., Takeshi, H., Ning, W., Tatsuhito, F. and Masashi, H. 2010. QTL mapping of clubroot resistance in radish. Theor. Appl. Genet. 120: 1021-1027. https://doi.org/10.1007/s00122-009-1230-z
  9. Arnold, D. L., Blakesley, D. and Clarkson, J. M. 1996. Evidence for the growth of Plasmodiophora brassicae in vitro. Mycol. Res. 100: 535-540. https://doi.org/10.1016/S0953-7562(96)80005-0
  10. Ashizawa, M., Yoshikawa, H. and Hida, K. 1980. Studies on the breeding of clubroot-resistance in cole crop. II. Screening of cole crops of clubroot-resistance (2). Bull. Vegetable and Ornamental Crops Res. Stn. A. 7: 35-75.
  11. Ayers, G. W. 1957. Races of Plasmodiophora brassicae. Can. J. Bot. 35: 923-932. https://doi.org/10.1139/b57-076
  12. Buczacki, S. T., Toxopeus, H., Mattusch, P., Johnston, T. D., Dixon, G. R. and Hobolth, L. A. 1975. Study of physiologic specialization in Plasmodiophora brassicae: Proposals for attempted rationalization through an international approach. Trans. Br. Mycol. Soc. 65: 295-303. https://doi.org/10.1016/S0007-1536(75)80013-1
  13. Cheah, L. H. and Page, B. B. C. 1995. Trichoderma spp. for potential biocontrol of clubroot of vegetable brassicas. Proc. 50th N. Z. Plant Protection Conf. 150-153.
  14. Cheah, L. H., Veerakone, S. and Kent, G. 2000. Biological control of clubroot on cauliflower with Trichoderma and Streptomyces spp. N. Z. Plant Protection 53: 18-21.
  15. Cho, W. D., Kim, W. G. and Takahashi, K. 2003. Occurrence of clubroot in cruciferous vegetable crops and races of the pathogen in Korea Plant Pathol. J. 19: 64-68. https://doi.org/10.5423/PPJ.2003.19.1.064
  16. Hirai, M., Harada, T., Kubo, N., Tsukada, M., Suwabe, K. and Matsumoto, S. 2004. A novel locus for clubroot resistance in Brassica rapa and its linkage markers. Theor. Appl. Genet. 108: 639-643. https://doi.org/10.1007/s00122-003-1475-x
  17. James, R. V. and Williams, P. H. 1980. Clubroot resistance and linkage in Brassica campestris. Phytopathology 70: 776-779. https://doi.org/10.1094/Phyto-70-776
  18. Johnston, T. D. 1968. Clubroot in Brassicae: A standard inoculation technique and the specification of races. Plant Pathol. 17: 184-187. https://doi.org/10.1111/j.1365-3059.1968.tb00452.x
  19. Kuginuki, Y., Yoshikawa, H. and Hirai, M. 1999. Variation in virulence of Plasmodiophora brassicae in Japan tested with clubroot resistance cultivars of Chinese cabbage (Brassica rapa L. ssp. pekinensis). Eur. J. Plant Pathol. 105: 327-332. https://doi.org/10.1023/A:1008705413127
  20. Kolte, S. J., Bordoloi, D. K. and Awarthi, R. P. 1991. The search for resistance to major diseases of rapeseed and mustard in India. GCIRC Rapeseed Congr. 6: 219-225.
  21. Komyoji, T., Sugimoto, K., Mitani, S., Matsuo, N. and Suzuki, K. 1995. Biological properties of a new fungicide, fluazinam. J. Pestic. Sci. 20: 129-135. https://doi.org/10.1584/jpestics.20.129
  22. Kuginuki, Y., Ajisaka, H., Yui, M., Yoshkawa, H., Hida, K. and Hirai, M. 1997. RAPD markers linked to a clubroot-resistance locus in Brassica rapa L. Euphytica 98: 149-154. https://doi.org/10.1023/A:1003147815692
  23. Kuginuki, Y., Yoshikawa, H. and Hirai, M. 1999. Variation in virulence of Plasmodiophora brassicae in Japan tested with clubroot resistant cultivars of Chinese cabbage (Brassica rapa L. ssp. pekinensis). Eur. J. Plant Pathol. 105: 327-332. https://doi.org/10.1023/A:1008705413127
  24. Matsumoto, E., Yasui, C., Ohi, M. and Tsukada, M. 1998. Linkage analysis of RFLP markers for clubroot resistance and pigmentation in Chinese cabbage. Euphytica 104: 79-86. https://doi.org/10.1023/A:1018370418201
  25. Mitani, S., Sugimoto, K., Hayashi, H., Takii, Y., Ohshima, T. and Matsuo, N. 2003. Effects of cyazofamid against Plasmodiophora brssicae Woronin on Chinese cabbage. Pest Mnag. Sci. 59: 287-293. https://doi.org/10.1002/ps.627
  26. Murakami, H., Tsushima, S., Akimoto, T., Murakami, K., Goto, I. and Shishido, Y. 2000. Effects of growing leafy daikon (Raphanus sativus) on population of Plasmodiophora brassicae (Clubroot). Plant Pathol. 49: 584-589. https://doi.org/10.1046/j.1365-3059.2000.00495.x
  27. Nakata, K. and Takimoto, K. 1928. List of disease of cultivated plants in Korea. J. Agric. Exp. Stn., Govern-Gen. Chosen 15: 77-78. (In Japanese)
  28. Peterka, H., Budahn, H., Schrader, O., Ahne, R. and Schutze, W. 2004. Transfer of resistance against the beet cyst nematode from radish (Raphanus sativus) to rape (Brassica napus) by monosomic chromosome addition. Theor. Appl. Genet. 109:30-41. https://doi.org/10.1007/s00122-004-1611-2
  29. Piao, Z. Y., Deng, Y. Q., Choi, S. R., Park, Y. J. and Lim, Y. P. 2004. SCAR and CAPS mapping of CRb, a gene conferring resistance to Plasmodiophora brassicae in Chinese cabbage (Brassica rapa ssp. pekinensis). Theor. Appl. Genet. 108: 1458-1465. https://doi.org/10.1007/s00122-003-1577-5
  30. Scheijgrond, W. and Vos, H. 1954. Investigation on the susceptibility to clubroot. Euphytica 3: 125-139. https://doi.org/10.1007/BF00029959
  31. Shimotori, H., Yanagida, H., Enomoto, Y., Igarashi, K., Yoshinari, M. and Umemoto, M. 1996. Evaluation of benzenesulfonanilide derivatives for the control of crucifers clubroot. J. Pestic. Sci. 21: 31-35. https://doi.org/10.1584/jpestics.21.31
  32. Suwabe, K., Tsukada, H., Iketani, H., Hatakeyama, K., Fujimura, M., Nunome, T., Fukuoka, H., Matsumoto, S. and Hirai, M. 2003. Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in Brassica rapa L. Theor. Appl. Genet. 107: 997-1002. https://doi.org/10.1007/s00122-003-1309-x
  33. Tanaka, S., Fujiyama, S., Shigemori, S., Nakayama, A., Ito, S. and Kameya, I. M. 1998. Pathogenesis of isolates of Plasmodiophora brassicae from Japan. (1) Race and pathogenesis in clubroot resistant cultivars. Proc. Assoc. Plant Protect. Kyushu 44: 15- 19. https://doi.org/10.4241/kyubyochu.44.15
  34. Tanaka, S., Kochi, S., Kunita, H., Ito, S. and Kameya, I. M. 1999. Biological mode of action of the fungicide, flusulfamide, against Plasmodiophora brassicae (clubroot). Eur. J. Plant Pathol. 105: 577-584. https://doi.org/10.1023/A:1008722005570
  35. Voorrips, R. E. 1996. Production and characterization of singlespore isolates of Plasmodiophora brassicae. Eur. J. Plant Pathol. 102: 377-383. https://doi.org/10.1007/BF01878132
  36. Williams, P. H. 1966. A system for the determination of races of Plasmodiophora brassicae that infect cabbage and rutabaga. Phytopathology 56: 624-626.
  37. Yoshikawa, H. 1981. Breeding for clubroot resistance in Chinese cabbage. In : Chinese Cabbage, ed. by N. S. Talekar and T. D. Griggs, pp. 405-413. Proc. 1st Int. Symp., Tsukuba, Japan.
  38. Yoshikawa, H. 1993. Studies on breeding of clubroot resistance in cole (Cruciferae) crops. Bull. Natl. Res. Inst. Veg. Ornam. Plants Tea. Japan. Ser. A. 7: 1-16.

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