• Title/Summary/Keyword: 접합방식

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염색체 미세절단과 형광 접합법을 이용한 소 성염색체 library의 개발

  • 이종호
    • Journal of Embryo Transfer
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    • v.14 no.1
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    • pp.3-5
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    • 1999
  • 이식 전 수정란의 성판정은 많은 축산인의 소망이었다. 많은 방법이 제기되었지만, 세포유전학적 분석방법은 ET의 상용화에 거의 무의미할 정도로 한계가 많았고, 이후 개발된 응성 특이적 항원이나 X-염색체에서 발현되는 효소의 활동을 통한 성판정 방법은 흥미롭기는 하나 산업적 적용에 제약이 많았다. 80년대 중반 Y-염색체 특이적인 DNA 탐침자의 개발과 PCR을 통한 DNA증폭기술의 개발은 수정란 성판정을 획기적으로 개선시켰다. DNA기술을 통한 수정란의 성판정은 분자생물학을 현장으로 진출시킨 첫 경우이기도 했다. 90년대에 세포유전학적 분석에서 FISH가 소개되었으며, 염색체 특이적 library는 다른 기초적이고 응용세포유전학적 연구에 유용한 도구가 되었다. 최근에는 사람에서는 착상전 수정란의 성판별 및 유전진단을 위해 실시되고 있는 형광직접접합법(fluorescent in situ hybridization, FISH)은 효소적 유전자 증폭(polymerase chain reaction; PCR)에 비해 높은 민감도와 정확성을 보이고 있으나 hybridization 및 washing 과정에 매우 긴 시간이 소요되고, 절차도 까다로워 현장에서의 적용이 용이치 않았다. 그러나 direct labelled probe의 이용, heat programmable instrument의 개발, denaturing chemical의 사용배제 등을 통해 소요시간 및 절차의 대폭적인 간소화를 이루어 현장의 적용 가능성을 한층 높이고 있다. 현재 사람의 세포유전학 및 종양학에서는 FISH의 다양한 기술이 많이 이용하고 있으나 소에서는 탐침자(probe)가 개발되어 있지 않아 그 이용이 미미하다. 본 연구는 FISH를 이용하기 위한 탐침자의 개발을 궁극적인 목표로 삼았으며, 이를 위해 접근이 용이한 방식을 개발하여 기존의 방식과는 다른 소 배아세포의 성을 판별 할 수 있는 접근방법을 소개 하고자한다.

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Case Studies of Domestic and Foreign Photovoltaic Integrated Projects (국내.외 PV 통합 프로젝트의 사례분석)

  • Yoon, Sung-Won;Kim, Hye-Seong
    • Journal of Korean Association for Spatial Structures
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    • v.8 no.6
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    • pp.85-94
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    • 2008
  • This paper is concerned with the domestic and foreign PV integrated projects in view of structural design. These projects are classified with public and display 6 facilities. The evaluated items are natural integration with building, engineering details and innovative new design. 6 domestic public and display facilities are frequently using isolated supporting system at roof, but show weakness in design integration. But the cases, using the PV panel in the stage of design some display facilities, are increasing. In the other hand, foreign public and display facilities are using the modul connection majorly and the structural and design integration are obtained.

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A study on chemical bonding characteristics of the interface between curved FRP panels for consecutive structural assembly (곡면 FRP 패널 부재 연속시공을 위한 연결부 화학적 접합 특성에 관한 연구)

  • Lee, Gyu-Phil;Shin, Hyu-Soung;Jung, Woo-Tai
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.14 no.1
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    • pp.79-91
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    • 2012
  • A curved fiber reinforced polymer (FRP) panel is produced with a certain width depending on allowances of manufacturing processes and facilities. An targeted arch-shaped structure could be built by sequential connection of series of the FRP panels. The connection manner between the FRP panels could be given by chemical treatment, mechanical treatment and hybrid method. Among those, the connection between the panels by chemical treatment is commonly adopted. Therefore, For an optimized design of the connected part between FRP pannels, a number of direct shear tests have been undertaken in terms of a number of parameters: surface treatment conditions, bonding materials, etc.. As results, surface grinding condition by sand paper or surface treatment by sand blasting appear properly acceptable methods, and epoxy and acryl resins are shown to be effective bonding materials for the purpose in this study.

Electrode bonding method and characteristic of high density rechargeable battery using induction heating system (유도 가열 접합 시스템을 이용한 대용량 이차전지 전극의 접합 방법 및 특성)

  • Kim, Eun-Min;Kim, Shin-Hyo;Hong, Won-Hee;Cho, Dae-Kweon
    • Journal of Advanced Marine Engineering and Technology
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    • v.38 no.6
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    • pp.688-697
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    • 2014
  • In this study, electrode bonding technology needed for high density of rechargeable battery is studied, which is recently researched for electric vehicle, the small leisure vessel. For the alternative overcoming the limit of stacking amount able to be stacked by conventional ultrasonic welding, the low temperature bonding method, eligible for minimum of degeneration of chemical activator on the electrode surface which is generated by thermal effect as well as the increase of conductivity and tension strength caused by electrode bonding using filler metal, not using conventional direct heating on the electrode material method, is studied. Specifically to say, recently used more generally the ultrasonic welding and spot welding method are not usable for satisfying stable electric conductivity and bonding strength when much electrode is stacking bonded. If the electrical power is unreasonably increased for the welding, due to the effect of welding temperature, deformation of electrode and activating material degeneration are caused, and after the last packaging, decline of electrical output and generating heat cause to reduce stability of battery. Therefore, in this study, induction heating system bonding method using high frequency heating and differentiated electrode method using filler metal pre-treatment of hot dipping are introduced.

Evaluation of high-velocity impact welding's interfacial morphology between Cu and CP-Ti using SPH numerical analysis method (SPH 해석기법을 이용한 Cu와 CP-Ti 고속 충돌 접합 단면의 형상학적 평가)

  • Park, Ki Hwan;Kang, Beom Soo;Kim, Jeong
    • Journal of Aerospace System Engineering
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    • v.13 no.2
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    • pp.34-42
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    • 2019
  • The existence of different thermodynamic properties results in various undesirable effects, such as thermal deformation and residual stress, in heat-welding processes. The solid-state junction, by using explosive or electromagnetic forces, i.e., high-velocity impact welding without employing heat is advantageous in joining materials with different thermodynamic properties. In the solid-state junction, the joining is performed within a short time, a high velocity and large deformations are accompanied by interfacial surfaces. The numerical analysis models play an important role in the understanding of the mechanism of high-velocity impact welding. However, in the analysis of high velocity and large deformations, the conventional Lagrangian method has low reliability due to the occurrence of entanglements. In this study, high-velocity impact welding between Cu and CP-Ti with different thermodynamic properties was performed using a un-gridded numerical method, SPH (Smoothed Particle Hydrodynamics), and interfacial morphology occurred. As a result of the analysis, the interfacial morphology was confirmed and the compared degree of shape (straight, vortex), period, length, and so on appeared differently depending on the relationship between the parameters (impact angle and speed).

An Experimental Study of Demountable Bolted Shear Connectors for the Easy Dismantling and Reconstruction of Concrete Slabs of Steel-Concrete Composite Bridges (강합성 교량의 콘크리트 바닥판 해체 및 재시공이 용이한 분리식 볼트접합 전단연결재에 관한 실험적 연구)

  • Jung, Dae Sung;Park, Se-Hyun;Kim, Tae Hyeong;Kim, Chul Young
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.42 no.6
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    • pp.751-762
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    • 2022
  • Welded head studs are mainly used as shear connectors to bond steel girders and concrete slabs in steel-concrete composite bridges. For welded shear connectors, environmental problems include noise and scattering dust which are generated during the removal of damaged or aged slabs. Therefore, it is necessary to develop demountable shear connectors that can easily replace aged concrete slabs for efficient maintenance and thus for better management of environmental problems and life cycle costs. The buried nut method is commonly studied in relation to bolted shear connectors, but this method is not used in civil structures such as bridges due to low rigidity, low shear resistance, and increased initial slip. In this study, in order to mitigate these problems, a demountable bolted shear connector is proposed in which the buried nut is integrated into the stud column and has a tapered shape at the bottom of an enlarged column shank. To verify the performance of the proposed demountable stud bolts in terms of static shear strength and slip displacement, a horizontal shear test was conducted, with the performance outcomes compared to those of conventional welded studs. It was confirmed that the proposed demountable bolted shear connector is capable of excellent shear performance and that it satisfies the slip displacement and ductility design criteria, meaning that it is feasible as a replacement for existing welding studs.

초고진공 펌프개발 -크라이오 펌프와 진공 브레이징의 이해-

  • Yu, Jae-Gyeong;Gang, Min-Jeong
    • Proceedings of the Korean Vacuum Society Conference
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    • 2010.08a
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    • pp.13-13
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    • 2010
  • 개요- 반도체 시장의 확대 및 소자의 고집적화와 장비의 초정밀화 등 고정도 생산성을 요구하는 현대 진공산업 시장에서는 정밀한 제품을 대량으로 생산할 수 있도록 지원할 수 있는 대용량 장비의 수요가 급증하고 있다. 서론- 하루가 다르게 발전하는 기술 경쟁 사회에서는 보다 성능이 좋은 제품을 다른 국가 또는 다른 업체와 차별화된 기술적 우위를 선점해야 한다. 본문-이에따라, 고신뢰도, 고생산성이 요구되는 진공산업 시장에 발맞추기 위한 일환으로 크라이오 펌프의 재생시간을 단축시켜 생산성 향상을 높이고, 기존의 제품에서 소비자가 느꼈던 진동을 최소로 줄일 수 있도록 저진동 제품의 설계를 통한 고객 만족의 제품을 개발함과 동시에, 맥동관 냉동기의 기술상업화를 꾀한 극저온기술 영역의 발전을 함께 도모하고자 한다. 현재까지는 국내의 일부 정부출연 연구소와 학교, 일부 특수산업 분야에서만 크라이오 펌프를 사용하고 있으나, 상업적인 설계 및 생산은 완전히 전무한 상태이다. 이는 진공시장의 잠재적인 성장을 고려할 때 국산화가 시급한 상황이다. 이러한, 상황을 고려하여 현재 기계연구원과 우성진공, 국민대학교가 맥동관 냉동기를 접목시킨 급속재생형 저진동 크라이오 펌프를 지난 2008년부터 산학연 협동으로 개발 진행 중에 있다. 크라이오 펌프의 핵심 기술은 냉동기의 효율에 있다고 볼 수 있으며, 이러한 냉동기의 효과를 높이기 위하여, 제작 공정에 진공 브레이징을 사용하여, 열교환기와 교환기 내의 충진재들과의 정확한 간극을 유지하면서 접합을 할 수 있는 환경요건을 지속적인 실험을 통해 지식을 습득하고 있다. 진공 브레이징은 외관을 아름답고 정교하게 접합할 수 있으므로, 추후 상품으로의 가치를 높일 수 있으며, 서로 다른 이종금속 간의 접합이 가능하여 열교환기의 무산소동과 스테인레스 튜브와의 접합을 가능하게 할 수 있었다. 진공브레이징에 쓰이는 모재는 여러가지가 있으며, 접합의 환경 또한 일반 용접보다 다양하므로 여러번의 실험을 통한다면, 성능은 우수하면서 외관으로 손색이 없는 개발품이 나올수 있겠다는 의견이 모아지고 있는 중이다. 본론- 아직까지는 미국의 CTI사나 일본의 ULVAC 등의 크라이오 펌프의 선진 기업에 비해 기술력이 많이 부족하기는 하지만, 아직 많은 부분이 미개발된 분야이고, 향후 후발국과의 차별을 두어 선진기술을 확보하기 위해서는 기존의 방식을 단순히 따라하는 것이 아닌 남들과 다른 원천기술의 확보가 더 필요하며, 이를 위해 부단한 노력과 시행착오를 거쳐 맥동관 냉동기형 크라이오 펌프라는 기술을 확보할 예정이다.

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Assessment of Optimum Reinforcement of Rebar for Joint of PHC Pile and Foundation Plate (고강도 콘크리트 말뚝과 기초판 접합부의 최적 철근보강량 산정)

  • Park, Jong-Bae;Sim, Young-Jong;Chun, Young-Soo;Park, Seong-Sik;Park, Yong-Boo
    • Land and Housing Review
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    • v.1 no.1
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    • pp.67-73
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    • 2010
  • Method of protruding steel bar embedded in PHC pile for connecting with foundation plate is an intermediate form of fixed and hinged connection and has often been used in architectural structures such as apartment complex. However, mechanical properties of this method have not been proved and its construction process is not simple. In this study, therefore, by analyzing previous research and by considering ratio of steel bar and concrete in PHC pile, which is minimum reinforcement of rebar, the newly optimized method of reinforcing joint of PHC pile and foundation plate is suggested with respect to PHC pile type (PHC 450, PHC 500, and PHC 600). To assess mechanical properties (ultimate tensile and shear strength) of joint of PHC pile and foundation plate, full scale experimental tests are performed. As a result, all cases are satisfied with required design criteria and can be practically applied. Our results indicate that reduction of rebar reinforcement compared to previous method would lead cost saving in PHC pile construction.

Prediction of Welding Pressure in the Non Steady state Porthole Die Extrusion of AI7003 Tubes (포트홀 다이 압출방식에 의한 AI7003 튜브의 접합강도예측)

  • Jo, Hyung-Ho;Lee, Sang-Gon;Lee, Seon-Bong;Kim, Byung-Min
    • Journal of the Korean Society for Precision Engineering
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    • v.18 no.7
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    • pp.179-185
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    • 2001
  • Porthole die extrusion is profitable to manufacture long tube with hollow section. The material through portholes is gathered within chamber and welded under high pressure. This weldability which classifies the quality of tube product is affected by several variables and die shape. But, porthole die extrusion has been executed on the experience of experts due to the complicated die assembly and complexity of metal flow. Analytic approaches that are useful in profitable die design and in the improvement of productivity are inevitably demanded. Therefore, the objective of this study is respectively to analyze the behavior of metal flow and to determine welding pressure of hot extrusion product according to the various billet temperature, bearing length and tube thickness by FE analysis and its results are compared with tube expanding tests.

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A Study on Wooden Assembly Methods Appeared in Kuma, Kengo's Works (쿠마 켄코의 건축에 나타나는 목재 접합방식에 관한 연구)

  • Uhm, Heechoon;Baik, Seung-Yeon;Park, Jin-Ho
    • Korean Institute of Interior Design Journal
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    • v.24 no.4
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    • pp.14-22
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    • 2015
  • This study deals with a comparative analysis of wooden assembly methods between Japanese traditional architecture and Kuma, Kengo's works. Kuma, Kengo has known as an architect who pursues Japanese architectural traditions with integrated solutions for built and natural environments, and expresses a deep commitment to unexpected use of materials such as stone and wood with the clarity of structural solutions. Accordingly, his architecture focuses on the appreciation of the spatial relationship with the surrounding, its local characters, and the selection of materials. This article first examines two of wooden assembly methods that have been shown in Japanese traditional architecture such as 'two-way system' and 'one-way system'. And then, Kuma, Kengo's wooden assembly methods in his works are analyzed and compared in relation to Japanese wooden assembly methods to find out similarities and differences between them. Then, it concludes that Kuma, Kengo's approach is not only relying on the methods of Japanese traditional architecture, but also exploring creative ways beyond traditional assembly methods.