• Title/Summary/Keyword: 전자현미경적 미세구조

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Ultrastructure of Cryptococcus neoformans in the Skin Tissue (조직내 Cryptococcus neoformans의 전자현미경적 관찰)

  • Seo, Young-Hoon;Kwon, Tae-Jung;Kim, Chung-Sook
    • Applied Microscopy
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    • v.12 no.1
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    • pp.49-56
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    • 1982
  • A case of systemic cryptococcosis developed in 4 year old boy was described and illustrated by light and electron microscope. Light microscopically, the upper dermis of the skin showed chronic nonspecific inflammation with numerous spherical spores surrounded by a clear halo created by the wide gelatinous capsule. Ultrastructurally, the C. neoformans showed the wide capsule containing microfibrils that appeared to radiate from the cell wall and to coil and interwine in various directions. The cell was uninucleate with a single nucleolus. Along the inner nuclear envelope, numerous small vesicles were present. In addition, C. neoformans presented membranous organelles derived from the plasma membrane and comparable to bacterial mesosomes.

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Electron Microscopic Study on Detoxication Effect of Red Ginseng Extract on Mouse Liver Injury induced by Methyl Mercury (메틸수은(水銀)으로 손상(損傷)된 생쥐 간장(肝臟)에 대한 홍삼(紅蔘) 추출물(抽出物)의 해독효과에 관한 전자현미경적(電子顯微鏡的) 연구(硏究))

  • Chung, Hee-Won
    • Applied Microscopy
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    • v.17 no.1
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    • pp.76-82
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    • 1987
  • Detoxication effect of red ginseng extract against toxicity of methly mercury on ultrastructure of mouse liver was studied by electron microscopy. The hepatocyte of methyl mercury treatment group showed partial rupture of nucleus, mitochondrial swelling, decrease of glycogen content, and appearance of a great number of large vacuoles. While, the hepatocyte of methyl mercury-red ginseng extract treatment group showed slight mitochondrial swelling and decrease of vacuoles in size and number than those of methyl mercury treatment group.

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An Electron Microscopic Study on the Changes of Rat Respiratory Mucosa by Passive Smoking (간접흡연으로 인한 흰쥐 호흡기점막의 변화에 대한 전자현미경적 연구)

  • 구본철;전진석
    • Biomedical Science Letters
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    • v.6 no.2
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    • pp.109-118
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    • 2000
  • The bronchus and alveoli from young rats have been examined by electron microscope following the exposure of cigarette smoking. Experimental animals were exposed to the sidestream smoke in an experimentally designed cage for 45 minutes per day during four weeks. In the smoking group, transmission electron micrographs of lung tissues showed a large number of neutrophils with electron densed several lysosomes, numerous macrophages with many small lysosomes, and many residual bodies in alveolar space. Scanning electron micrographs revealed that the ciliated epithelial cells in bronchus of smoking group were replaced by goblet cells including loss of cilia, and increased cell size of many goblet cells in bronchus. These results depicted that the ultrastructural changes are due to the passive smoking, involving airway cell Injury.

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경북동해안산화석규조의 미세구조에 관한 전자현미경적관찰

  • 안영필
    • Journal of Plant Biology
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    • v.3 no.2
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    • pp.26-28
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    • 1960
  • Electron-Microscopical Observalion on Fine Structure of Diatom Frustules which is collected in the East Coast of Kyung-Pook Province. Kor. Jour. Bot. 3(2) 26-28 lllus. 1960 The fine structure of Diatom frustules which is collected in the east coast of Yungil and Wul Sung, Kyung-Pook Province, is ovserved by the light microscope and hte electron-microscope, and the materials collected by auther are 1 family, 2 genus and 2 species. Coscinodiscus elegans. Greville Actinoptychus undulatus Rals Those were havitated in sea water as the plankton which were found in diatomite.

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인삼 캘러스로부터 분이 및 배양된 원형질체의 전자현미경적 연구

  • 박종범
    • Journal of Plant Biology
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    • v.37 no.2
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    • pp.183-193
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    • 1994
  • 인삼(Panax ginseng C.A. Meyer)의 뇌두 캘러스로부터 분리한 원형질체와 배양된 원형질체의 미세구조 변화와 원형질막 표면을 투과 및 주사전자현미경으로 조사하였다. 분리 직후의 원형질체에서는 캘러스세포에서보다 작은 액포들이 많이 형성되어 있었다. 또한 활면소포체의 수가 증가하였으며 이들은 원형질막과 평행으로 배열하였다. 활면소포체는 가끔 세포질을 둘러싸서 세포질분리체(cytosegresome)를 형성하였고, 이 구조는 세포질을 분해시킨 후 액포로 변화하기도 하였다. 배양된 원형질체에서는 분리 직후의 원형질체와 비교하여 조면소포체, 딕티오좀, 리보좀, 미토콘드리아, proplastid 및 액포 등의 수가 현저하게 증가하였다. 딕티오좀으로부터 많은 소낭들이 형성되었고 이들은 세포질 전반에 걸쳐 존재하였다. 때로는 소낭들이 원형질막 바깥으로 돌출되어 돌기를 형성하기도 하였다. 배양된 원형질체의 표면에는 섬유소로 구성되어 있을 것으로 추정되는 섬유상 구조들이 형성되어 있었다.

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Ultrastructural Study of Drosophila Ocellar Visual System by Osmium Impregnation (오스뮴침착법에 의한 초파리 단안시각계의 미세구조)

  • Yoon, Chun-Sik
    • Applied Microscopy
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    • v.29 no.4
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    • pp.451-457
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    • 1999
  • Ultrastructure of adult Drosophila ocellus was compared with conventional electron microscopic method and osmium impregnation. When osmium impregnation was applied, some organelles of cells were strongly stained. Especially, subrhabdomeric cisternae (SRC) were strongly stained and showed network-iike structure as in compound eye. Other organelles including SSC, ER, nuclear envelope, pigment granules and mitochondria were also strongly stained. These organelles are known as a general calcium ion reservoir. In conclusion, the strong effect of light and shade by osmium impregnation was regarded as a result of strong binding between calcium ion and osmium tetroxide. Thus, we agree to the opinion that osmium impregnation is very useful methods to the comparative morphology of cell organelles.

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Effects of Di-(2-ethylhexyl) phthalate (DEHP) on Ultrastructure of Rat Seminal Vesicle (Di-(2-ethylhexyl)phthalate (DEHP)가 흰쥐 저정낭의 미세구조에 미치는 영향)

  • Kil, Young-Chun;Kim, Wan-Jong;Shin, Kil-Sang
    • Applied Microscopy
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    • v.30 no.1
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    • pp.73-80
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    • 2000
  • Di-(2-ethylhexyl)phthalate (DEHP) has been known as one of endocrine disruptors. The present study was carried out to investigate the alterations of fine structure in rat seminal vesicle after oral intubation of DEHP in dosages of 1g/kg/day, 2g/kg/day or 3g/kg/day respectively in 0.5 ml of corn oil for If days. In rats treated with DEHP for 15 days, seminal vesicle exhibited extensive histological alterations compared to those observed in control groups. The size of the seminal vesicle and the mucosal folds decreased, but the lamina propria was considerably thickened. The ultrastructural changes of epithelial cells in seminal vesicle of rat treated with DEHP were characterized by the high nuclear/cytoplasmic ratio and the increased beterochromatin within irregular nuclear envelope. And also, the rough endoplasmic reticulum, Golgi complex and secretory vesicles were poorly developed. In conclusion, DEHP caused the ultrastructural and functional alterations of seminal vesicle in rats dose-dependently. It is suggested that these detrimental effects of DEHP on seminal vesicle are derived from the decrease level of testosterone.

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