• Korean Chemical Engineering Research
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• v.58 no.3
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• pp.450-457
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• 2020

In the previous study, there was a big difference between the tendency of the delivery efficiency of Yo-Pro-1 and the expression efficiency of the CFP gene, but there was a problem that could not provide a clue to this problem. Therefore, this study aimed to present a clue to this problem by quantifying and comparing the delivery efficiency after labeling DNA using a fluorescent dye, which was one of the methods for quantifying biomolecules. As a fluorescent dye for labeling, Yo-Pro-1 was used, and the delivery efficiency of the fluorescent dye Yo-Pro-1 and the labeled DNA was compared. The delivery efficiency of Yo-Pro-1 and labeled DNA according to the voltage condition of the digital electroporation system was maximized at 96 V, and the delivery efficiency tended to decrease as the voltage increased further. In the comparison of the delivery efficiency of Yo-Pro-1 and labeled DNA according to the number of voltage application conditions, the delivery efficiency was maximized at the number of 8 voltage application times for both delivery materials, and the delivery efficiency tended to decrease as the number of voltage application increases further. Through the two results, it was confirmed that the delivery efficiency using Yo-Pro-1 in the digital electroporation system represents the delivery efficiency of the system well. In addition, through the results of this study, the difference between the tendency of the delivery efficiency of Yo-Pro-1 and the expression efficiency of the CFP gene shown in the preceding study was not the result of the difference in the delivery efficiency of the delivery material, but it can be predicted to be due to a problem with the expression process of the genetic material that had been delivered.

• Proceedings of the Korea Contents Association Conference
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• 2015.05a
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• pp.313-314
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• 2015

노인복지서비스 전달체계는 서비스 내용이 만들어지고 그것이 수여자에게 전달되는 과정 모두를 포함한 개념이다. 고령화가 급속하게 진행되는 현실에서 무엇보다 필요한 것은 노인복지에 대한 적절한 정책적 대안이 제시되고 전달될 수 있도록 노인복지서비스 전달체계 관리의 효율화가 이루어 져야 한다. 효율적인 노인복지서비스를 전달하기 위해서는 서비스 내용뿐만 아니라 서비스를 전달하는 공급자 중심에서 수요자 중심으로의 전환이 요구되며, 서비스를 전달하는 체계 또한 시대의 흐름에 따라 적절히 대응할 필요가 있다. 본 연구에서는 우리나라 노인복지서비스가 어떻게하면 효율적으로 수요자에게 전달될 수 있을 것인가를 연구하여 제시하고자한다.

• Journal of Korean Society of Steel Construction
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• v.16 no.5 s.72
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• pp.695-703
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• 2004

In this paper, the results of monotonic loading analysis with four steel models and one composite model were shown. The effect that moment transfer efficiency of a web and strain concentration at a steel beam-to-column connections was investigated. Analysis results showed that the moment transfer efficiency of the analytical model with box-column was poor when comparing to model with H-column due to out-of-plane deformation of the box-column flange. The presence of scallop, thin plate of box column and floor slabs was also a reason of the decrease of moment transfer efficiency, which would result in a potential fracture of the steel beam-to-column connections. Analytical results were compared with experimental results based on previous test. As a result, the deformation capacity of connections with a box-column or a floor slab decreased due to the poor moment transfer efficiency and the strain concentration of beam flange in the vicinity of the steel beam-to-column connections based on the experimental data.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2003.05b
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• pp.393-394
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• 2003

최근 들어 질소산화물 (NOx)을 저온 플라즈마로 처리하려는 연구가 활발하게 이루어지고 있다. 플라즈마 공정의 단점 중 하나는 다른 공정에 비해 비교적 에너지 소모가 높다는 것인데, 전원 (wall plug)에서 나오는 전력 중 일부만이 플라즈마를 발생시키는데 이용되어, 에너지 효율이 낮은 것으로 보고되고있다. 따라서 플라즈마 공정을 실제 공정에 적용하기 위해 선결되어야할 과제는 에너지 효율을 높이는 것이다. 이러한 에너지 효율은 가스의 조성과 인가 전압 등에 많은 영향을 받는다. 본 연구에서는 교류 유전체 장벽 방전 (Dielectric Barrier Discharge)의 가스 조성과 인가 전압을 변화시키면서 전원에서 반응기로 전달되는 에너지 전달 효율을 살펴보았으며, 에너지 전달효율과 NO의 전환이 상관관계를 살펴보았다. (중략)

• Journal of the Earthquake Engineering Society of Korea
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• v.10 no.4 s.50
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• pp.67-76
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• 2006

In this paper the moment transfer efficiency of a web and the strain concentration at the RHS (Rectangular Hollow Section) column-to-steel beam connections was evaluated. Initially, non-linear finite element analysis of five bare steel beam models was conducted. The models were designed to have different detail at their beam-to-column connection, so that the flexural moment capacity was different respectively. Analysis results showed that the moment transfer efficiency of the analytical model with RHS-column was poor when comparing to model with WF(Wide Flnage)-column due to out-of-plane deformation of the RHS-column flange. The presence of scallop and thin plate of RHS column was also a reason of the decrease of moment transfer efficiency, which would result in a potential fracture of the steel beam-to-column connections. Analytical results were compared with the previous experimental results. The analytical and the previous experimental results showed that the strain concentration was inversely proportional to the moment transfer efficiency of a beam web and the deformation capacity of connection was poor as their moment transfer efficiency degrades. Further finite element analyses of composite beam with a floor slab revealed that the neutral axis moved toward the top flange and the moment transfer efficiency of a beam web decreased, which led to premature failure of the connection.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.02a
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• pp.242-242
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• 2011

현재 반도체 및 디스플레이 장비들이 공정 매개변수 및 플라즈마 변수를 독립적으로 제어하기 위하여 전원 주파수를 다양하게 사용된다. 플라즈마의 상태나 에너지 전달 효율은 반도체 및 디스플레이 공정에 중요한 요소이다. 따라서 플라즈마 발생장치의 전원 주파수를 바꾸었을 때의 플라즈마 밀도와 에너지 전달 효율에 관하여 연구하였다. 공정용 유도 결합 플라즈마(ICP)를 발생시키기 위하여 신호 발생기에서 전력 증폭기와 임피던스 정합회로(Matcher)를 거쳐 반응 용기에서 플라즈마를 발생시켰다. 6 mTorr의 압력에서 주파수는 13.56 MHz에서부터 80 MHz까지, 15~60 W의 전력을 인가하였다. 플라즈마의 에너지 효율을 측정은 제작한 로고스키코일(Rogowski Coil)을 이용하여 시스템 전반을 등가회로로 계산하였으며, 플라즈마 밀도는 반응용기 중앙에서 부유 탐침법을 적용하여 도출하였다. 같은 전력 조건에서 주파수가 증가함에 따라 플라즈마 밀도가 증가함을 볼 수 있었다. 그러나 플라즈마 에너지 효율은 주파수가 높아짐에 따라 점점 커지다 작아지는 경향을 볼 수 있었다. 에너지 전달 효율의 변화는 정합회로의 표피효과(Skin effect)에 기인하며 플라즈마 밀도의 변화는 이온의 에너지 손실에 기인한다.

• Korean Chemical Engineering Research
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• v.56 no.1
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• pp.79-84
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• 2018

In the present study, we performed electroporation to deliver protein into microalgae using previously developed digital electroporation system. Green fluorescence protein was successfully delivered into a live microalgae cell nucleus without cell wall removal. By investigating the effects of applied voltage on the protein delivery efficiency, optimal electroporation electric field condition was found (960 V/cm). We also investigated the delivery of Yo-Pro-1 into cell to examine the size effects of delivered materials and found that there is little size effects on the optimal condition. Finally, the implications of the present results and future work are discussed.

• Clinical and Experimental Pediatrics
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• v.50 no.10
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• pp.1011-1017
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• 2007

Purpose : We tried to assess the optimal conditions to improve low transduction efficiency and their effect on target cells. Methods : Cultured NIH 3T3 cells were incubated with retroviral vectors bearing an enhanced green fluorescent protein (eGFP) gene. We varied the ratio of viral vectors to target cells (1:1-1:8) and the number of transfections (${\times}1$, ${\times}2$), and compared transduction efficiencies. Also, the effects of polybrene on transduction efficiency and viability of target cells were assessed. Transduction of the eGFP gene was evaluated by observing NIH 3T3 cells under a fluorescence microscope and efficiencies were measured by the percentage of eGFP positive cells using FACscan. Results : As the ratio of retroviral vectors to target cells increased, transduction efficiency was greatly improved, from 7% (1:1) to 38% (1:4). However, transduction efficiency did not increase any more when the ratio increased from 1:4 to 1:8. Cells transfected twice showed higher transduction efficiencies than cells transfected once, at a ratio of 1:8. The eGFP gene transduced to NIH 3T3 cells sustained its expression during repeated passages. However, after the third passage (day 9), the percentage of eGFP positive cells began to decline. The degree of this decline in eGFP expression was lower in cells transfected twice than in cells transfected once (P<0.05). The addition of polybrene did not have any toxic effect on NIH 3T3 cells and greatly increased transduction efficiency (P=0.007). In addition to vector component, transduction efficiency was very sensitive to culture confluence. Cells cultured and transfected in 24-well plate showed higher transduction efficiency, although cells cultured in 6- well plate proliferated more (P=0.024). Conclusion : Our data could be used as a basis for retrovirus-based gene therapy. Further study will follow using human cells as target cells.

• Journal of the Korean Geotechnical Society
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• v.22 no.12
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• pp.33-43
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• 2006

Driving tests using model plastic piles with different hammer cushion materials were performed in order to evaluate the efficiency of energy transfer ratio from the hammer, degree of vibration of the surrounding ground and noise due to impacting. A small pile driving analyzer (PDA) was composed using straingages and Hopkinson bar which is measuring force signal and pile-head velocity. The hammer cushion (cap block) materials used for the model driving tests were commercial Micarta, plywood, polyurethane, rubber (SBR) and silicone rubber. The highest energy transfer ratio was obtained from Micarta in the same soil and driving conditions. Micarta was followed by polyurethane, plywood, rubber and silicone in descending order. The more efficient energy transfdr ratio of the hammer cushion materials became, the bigger average noisy (sound) level was found. In addition, Micarta and polyurethane provided bigger bearing capacities than other materials compared in the same soil and driving conditions in which the static loading tests were performed at the end of driving.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2016.11a
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• pp.122.1-122.1
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• 2016

관상동맥 경화나 말초 혈관 동맥 경화 등이 발생한 경우 스텐트를 이용한 치료나 혈관 접합술(bypass grafting surgery)에 의한 치료를 하게 된다. 그러나 많은 경우 치료 부위에 발생한 혈관 조직에의 손상으로 인해 재협착(restenosis)이나 폐색(occlusion)이 일어나 환자의 생명을 위협하는 치명적인 결과를 유발하는 경우가 종종 발생한다. 이러한 재협착이나 폐색은 혈관민무니근세포(smooth muscle cells)의 이상성장(abnormal growth) 때문인데, 이를 억제하기 위한 다양한 약물이 개발되어 왔으나 치료 대상 부위에 높은 효율로 2~3 주간의 기간 동안 약물 전달하기가 어려운 실정이다. 최근 혈관접합 부위 (anastomosis site)등에 적용할 수 있는 메쉬나 실린더 형상의 약물전달 디바이스들이 개발되어 왔으나 약물 전달의 효율 등에서 더 개선이 절실히 필요한 실정이다. 본 발표에서는 혈관 외벽에 장착되어 혈관 중간막 (tunica media) 조직으로의 약물 전달 효율을 높이기 위해 마이크로니들(microneedles)을 이용한 디바이스들을 개발하고 약물 전달 성능과 치료효과를 소개하고자 한다. 열인장 공정 (thermal drawing)과 트랜스포 몰딩(transfer molding) 등의 마이크로 니들 제작공정을 설명하고 이를 바탕으로 제작된 커프(cuff) 형 및 유연 메쉬 (flexible mesh) 형의 디바이스 개발 과정을 소개하고자 한다. 특히, 이 디바이스들의 동물실험을 통한 약물 전달 효율의 향상 및 치료 효과의 증대에 대한 논의를 하고자 한다.