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http://dx.doi.org/10.9713/kcer.2018.56.1.79

Delivery of Protein into Microalgae by the Digital Electroporation  

Im, Do Jin (Department of Chemical Engineering, Pukyong National University)
Publication Information
Korean Chemical Engineering Research / v.56, no.1, 2018 , pp. 79-84 More about this Journal
Abstract
In the present study, we performed electroporation to deliver protein into microalgae using previously developed digital electroporation system. Green fluorescence protein was successfully delivered into a live microalgae cell nucleus without cell wall removal. By investigating the effects of applied voltage on the protein delivery efficiency, optimal electroporation electric field condition was found (960 V/cm). We also investigated the delivery of Yo-Pro-1 into cell to examine the size effects of delivered materials and found that there is little size effects on the optimal condition. Finally, the implications of the present results and future work are discussed.
Keywords
Digital electroporation; Microalgae; Protein delivery; CRISPR/CAS9;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
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