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Molecular Phylogeny of Phytoplakton Isolated from Red Tides in Southern Coast of Korea (국내 남해안에 발생한 적조원인생물들의 24S rRNA 유전자 염기서열분석)

  • Lee, Soo-Woong;Lee, Hee-Woo;Park, Jong-Gyu;Lee, Jin-Ae;Park, Young-Shik
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.3 no.2
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    • pp.90-93
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    • 1998
  • Cultured isolates of Prorocentrum minimum, P. micans, P. triestinum, P. balticum, Gymnodinium sanguineum, Alexandrium catenella, Scrippsiella trochoidea, and Heterosigma akashiwo from red tides in southern coast of Korea were phylogenetically compared on the basis of their 24S rRNA genes. For each isolate approximately 700 bp of 24S rDNA, encompassing D1 and D2 hypervariable domains, was amplified using the polymerase chain reaction and sequenced. The sequences were aligned with those reported in Genbank by using ClustalW program and phylogenetic tree was generated to show that morphospecies designations in the Alexandrium and Prorocentrum species are congruent with terminal taxa defined by phylogenetic analysis of the 24S rRNA fragment. Our results suggest that the molecular phylogeny approach could facilitate identification of domestic dinoflagellates which have ambiguous morphologies.

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The Influences of Extracts from Ceratium spp, on the Growth of Harmful Microalgae (유해성 적조생물에 대한 Ceratium 추출물 영향 평가)

  • Cho, Eun-Seob
    • Journal of Life Science
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    • v.14 no.4
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    • pp.589-592
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    • 2004
  • The growth response of the fish-killing dinoflagellate Cochlodinium polykrikoides was studied in cultures, using the treatment of Ceratium extracts by a methanol, a water-soluble, and a cell-free medium. The cell-free medium had the most increasing on the growth of C. polykrikoides cultures, enriched with $\geq$ 25% Ceratium, whereas the methanol and water-souble fractions did not affect the growth of C. polykrikoides exposed to even higher concentration. In particular, the cell-free medium also increased the growth of Gyrodinium impudicum and Chaetoceros sp., similar species to C. polykrikoides. In contrast to C. polykrikoides, G. impudicum and Chaetoceros sp., the growth of Alexandrium tamarense was inhibited significantly, and there was no great effect on the growth of Prorocentrum minimum. These results imply that Ceratium extracts may play an important role in the stimulatory effect of C. polykrikoides, and they have to affect the interaction between C. polykrikoides and Ceratium when co-existing.

An Experimental Study on Strength and Ductility of Masonry Buildings Retrofitted by Metal Connectors (조적조 건물의 연결철물보강에 따른 내력 및 연성에 관한 실험적 연구)

  • Park, Byung-Tae;Kwon, Ki-Hyuk
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.19 no.3
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    • pp.113-121
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    • 2015
  • Building using masonry accounts for most of the smaller houses in Korea but due to brittle behavior and low ductility the frequency of usage has decreased in recent years. Despite this, this form of building has been gaining attention overseas for its low cost in construction and environment-friendliness of the materials. As such, many studies are being conducted to resolve the disadvantages in structure. This study produced an specimen for masonry-filled wall and the intersection to confirm the difference in structural movement depends on the existence or lack of expansion joint and verified the reinforcement effect from inserting a connecting steel item (steel plate, stainless steel twist bar). The experiment results show that the specimen with a steel plate inserted saw an increase in durability and an improvement in the strength of the specimens, while the specimen that had stainless steel twists bar inserted saw an increase in ductility that did not cause brittle failure, indicating that the reinforcement effects of inserting a connecting steel item are effective.

An Analysis on the Correlation between Crack Condition and Safety Grades in Masonry Buildings (조적조 건축물의 균열양태 및 등급판정과의 상관성에 관한 연구)

  • Kwon, Ki-Hyuk
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.9 no.3
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    • pp.101-110
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    • 2005
  • In Seoul, structural deterioration is severe in the private masonry buildings which have been built since 1906s. But most of these structures remain without any repair works. As a result, the rate of deterioration is getting faster and these dangerous structures may cause hazardous circumstances to the adjacent structures and neighborhood. The purpose of this study is to investigate the actual conditions of wall cracks among the defect types which occur in private masonry buildings and to analyze the correlation between safety grades and wall cracks for offering the fundamental data. Using these date we can establish basic criteria for safety grades of structures and improve the quality of masonry buildings. The result of this study indicate that there are high correlations between safety grades and the width of crack but much less so with the length. Furthermore, with regard to crack patterns, vertical cracks much more negatively effected the safety grades.

An Estimation of Shear Capacity of Hexagonal Masonry Walls Under Cyclic Loading (반복하중을 받는 육각형 블록 벽체 전단내력평가)

  • Chang, Gug-Kwan;Seo, Dae-Won;Han, Tae-Kyoung
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.14 no.6
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    • pp.205-214
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    • 2010
  • Masonry structures have been used throughout the world for the construction of residential buildings. However, from a structural point of view, the masonry material is characterized by a very low tensile strength. Moreover, the bearing and shear capacity of masonry walls have been found to be vulnerable to earthquakes. In this study, to improve the seismic performance of masonry walls, hexagonal blocks were developed and six masonry walls made with hexagonal block were tested to failure under reversed cyclic lateral loading. This paper focuses on an experimental investigation of different types of wall with hexagonal blocks, i.e. walls with different hexagonal blocks and with different reinforcing bar arrangements, subjected to applied cyclic loads. The cracking, damage patterns and hysteretic feature were evaluated. Results from the hexagonal masonry wall were shown more damage reduction and less brittle failure in comparison to the existing rectangular masonry walls.

Performance Evaluation for Deteriorated Masonry in Military Facilities (조적조 노후 군시설의 성능 평가)

  • Yang Eun-Bum;Shin kyoung-Hee;Hwang Jong-Hyun;Kim In-Ho;Kim Yong-In;Park Tae-keun;Lee Chan Shik
    • Proceedings of the Korean Institute Of Construction Engineering and Management
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    • autumn
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    • pp.434-438
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    • 2001
  • Military facilities with masonry construction have a great portion in the whole military facilities. But lots of them have been used for more than 20 years, the degree of deterioration of the facilities are very serious. Futhermore, as small budget for the facilities maintenance and poor maintenance, the performance of the aged masonry facilities have continually decreased. We suggest a structural performance assesment criteria for the military facility through literature review, interview with experts and questionnaire. The assesment of structural performance includes the inclining and sinking degree of the facilities, durability of materials and resisting force of the structural members.

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Diel migration of Dinoflagellates, Cochlodinium polykrikoides in situ (Dinoflagellates, Cochlodinium polykrikoides의 현장 일부이동)

  • 나기환;박경대;이숙희;김건효;남정배
    • Journal of Aquaculture
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    • v.10 no.4
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    • pp.457-462
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    • 1997
  • During the period of the Cochlodinium polykrikoides red tide occurrence in the southern coast of Korea in 1997, we obseved the vertical migration of red tide organisms in situ, along with environmental factors such as water temperature, dissolved oxygen, pH, chlorophyll $\alpha$ and nutrients for practical application of loess suspension techniques ot diminish fisheries damage. To cope with serious outbreaks of red tide of C. polykrikoides, the finfish culturists resorted with loess suspension to disperse the bloom watermass by turning around it with fishing boats in full speed. Peak in afternoon the cell numbers were diminished in the surface layer after sunset red tide organisms settled down in the middle and bottom layer progressively again after sunrise. Chlorophyll $\alpha$ and dissolved oxygen showed the same trends of variation with the cell number. In the course of controlling the red tide with loess suspension we could not find any severs oxygen deficiency to kill the culture species.

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Study on Detection Technique for Cochlodinium polykrikoides Red tide using Logistic Regression Model and Decision Tree Model (로지스틱 회귀모형과 의사결정나무 모형을 이용한 Cochlodinium polykrikoides 적조 탐지 기법 연구)

  • Bak, Su-Ho;Kim, Heung-Min;Kim, Bum-Kyu;Hwang, Do-Hyun;Unuzaya, Enkhjargal;Yoon, Hong-Joo
    • The Journal of the Korea institute of electronic communication sciences
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    • v.13 no.4
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    • pp.777-786
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    • 2018
  • This study propose a new method to detect Cochlodinium polykrikoides on satellite images using logistic regression and decision tree. We used spectral profiles(918) extracted from red tide, clear water and turbid water as training data. The 70% of the entire data set was extracted and used for model training, and the classification accuracy of the model was evaluated by using the remaining 30%. As a result of the accuracy evaluation, the logistic regression model showed about 97% classification accuracy, and the decision tree model showed about 86% classification accuracy.

Host-Parasite System in a Red Tide Dinoflagellate Prorocentrum minimum: (1) Life Cycle Stages of the Parasitic Dinoflagellate Amoebophrya sp. (적조생물 Prorocentrum minimum의 숙주-기생자 배양체: (1) 기생성 와편모류 Amoebophrya sp.의 생활사 단계)

  • 김영길;박명길;이원호
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.7 no.4
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    • pp.2221-2221
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    • 2002
  • The first laboratory culture of host-parasite system of Prorocentrum minimum- Amoebophrya sp. was established by single cell isolation method. Here, we report the life cycle stages of the parasitic dinoflagellate. Amoebophrya sp. of the red tide dinoflagellate P. minimum as observed by light and epifluorescence microscopy. Infections developed inside the nucleus of P. minimum. The trophont developed to occupy almost all the intracellular space of the host at its late stage. The fully developed trophont finally ruptured through the host cell. “Vermiform stage”, the free-swimming extracellular lift cycle stage is followed by another stage for the sudden release of many individual dinospores. Our laboratory strain of the host-parasite system for P. minimum, a causative species fur the huge red tides in spring and summer in Korean coastal waters, could be a useful living material for the in situ biological control of harmful algal blooms.

Host-Parasite System in a Red Tide Dinoflagellate Prorocentrum minimum:(1) Life Cycle Stages of the Parasitic Dinoflagellate Amoebophrya sp. (적조생물 Prorocentrum minimum의 숙주-기생자 배양체: (1) 기생성 와편모류 Amoebophrya sp.의 생활사 단계)

  • 김영길;박명길;이원호
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.7 no.4
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    • pp.221-225
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    • 2002
  • The first laboratory culture of host-parasite system of Prorocentrum minimum- Amoebophrya sp. was established by single cell isolation method. Here, we report the life cycle stages of the parasitic dinoflagellate. Amoebophrya sp. of the red tide dinoflagellate P. minimum as observed by light and epifluorescence microscopy. Infections developed inside the nucleus of P. minimum. The trophont developed to occupy almost all the intracellular space of the host at its late stage. The fully developed trophont finally ruptured through the host cell. “Vermiform stage”, the free-swimming extracellular lift cycle stage is followed by another stage for the sudden release of many individual dinospores. Our laboratory strain of the host-parasite system for P. minimum, a causative species fur the huge red tides in spring and summer in Korean coastal waters, could be a useful living material for the in situ biological control of harmful algal blooms.