• Fashion & Textile Research Journal
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• v.15 no.1
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• pp.138-146
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• 2013

Chemical compositions and biological functions of brown colorants extracted from pine bark(Pinus densiflora) have been studied. Dyeing test using multifiber fabrics with extracted colorants were preliminary carried out. Dyeing conditions and fastness tests of selected fabrics have been also studied. The brown colorants were produced 1.5% concentrations by solvent extraction from milled pine bark using methanol. The colorants were extracted with 80% methanol as best choice by a criteria of solid quantity and dyeability on fabrics. The chemical compositions were identified as mixtures of taxifolin epicatechin and procyanidin by LC/MS analysis. The brown colorants could be dyed not only natural fibers such as cotton, silk and wool but also synthetic fiber as nylon and semi-synthetic fiber as viscose rayon. Maximum K/S values was shown at 400 nm according to different fiber with color appearance of redish brown. Optimum pH and temperature of dyeing conditions was 4 and above $80^{\circ}C$, respectively. The brown colorants had a strong antioxidant activity compared to Butylated hydroxyanisole as standard and weak antimicrobial activity against E. coli. compared to kanamycin. Washing, rubbing, perspiration, dry cleaning and light fastness for cotton, nylon and silk dyed with the brown colorants were carried out by KS K method. Most of color fastness such as washing, rubbing, perspiration, and dry cleaning were represented as 4-5 grade. However, light fastness was reported as 2-3 grade. From this studies, brown colorants produced pine bark have a high potentials for natural dyeing on fabrics with antioxidant activity.

• Applied Biological Chemistry
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• v.41 no.1
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• pp.95-98
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• 1998

Methanol extracts of 54 plant species in 32 families were tested for their antignawing activities against mice by wire-dipping method. The activity varied with plant species. Potent antignawing activity was observed in extracts from roots of Aucklandia lappa (Compositae), barks of Cinnamomum cassia (Lauraceae), fruits of Illicium verum (Magnoliaeceae), fruits of Piper nigrum (Piperaceae), rhizomes of Rheum officinale (Polygonaceae), and leaves of Pinus densiflora (Pinaceae). As naturally occurring rodent repellents, these plant-derived materials could be useful as a preventive agent against various kinds of damage caused by rodents.

• Journal of Korean Society of Forest Science
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• v.67 no.1
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• pp.31-41
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• 1984

This study was examined for purposes of identifing woods of the hull construction collected from a sunken old-ship by the anatomical wood features, and then from the result the geographical distribution ranges of the species were described. Most woods of the hull construction, i.e. shell plating, bulkhead, floor, bulwark, deck and kneel were identified with Chinese red pine (Pinus massoniana), but the fenders were idendified with Chinese fir (Cunninghamia lanceolata). These two species are known to be the endemic species in the south region of Yangtze River. As the other materials, bulwark stays and liquid tank stays were respectively identified with Cinnamomum spp., Distylium spp., and the geographical distributions of these species are the south China, the south coast of Korea and the Japanese Islands.

• The Korean Journal of Mycology
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• v.8 no.1
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• pp.7-11
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• 1980

To increase production of Tricholoma matsutake, experiments were carried out in a mountain located in Keumma 2-ri, Joocheon-myun, Yongwol-gun, Kangwon-do, Korea during the period from April to November 1979. The area of the experiment showed pH 6.55 of soil acidity and the rock was found to be granite. Among its vegetation, the major growths were Pinus densiflora Siebold et Zuccarini, Rhododendron mucronulatum Turcz., Rhododendron schlippenhachii Max., Lespedeza bicolor and Pteridium aquilinum (Linne) Kuhn var. japonicum Nakai. Of two equally divided areas of A and B in which pine mushrooms grew, artificial methods such as thinning the upper portion of land raking and water-spraying were applied to area B only, where 21% increase of the production was obtained as compared with the control area A.

• Korean Journal of Pharmacognosy
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• v.47 no.3
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• pp.246-250
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• 2016

Pinus densiflora contained diverse phenol compounds like flavonoid, phenylpropanoid and tannin. PineXol$^{(R)}$ is nutraceutical preparation which was treated from bark of Pinus densiflora. Validation and contents determination of taxifolin, (+)-catechin and procyanidion B1 for the preparation of Pinus densiflora (PineXol$^{(R)}$) were confirmed using High-Performance Liquid Chromatography (HPLC). As a result, content of taxifolin, (+)-catechin and procyanidin B1 were, respectively 4.90%, 2.35% and 8.19%. These analysis method and results could be used as important basic data for the preparation of Pinus densiflora.

• Korean Journal of Food Science and Technology
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• v.30 no.1
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• pp.213-217
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• 1998

This study was performed to observe cytotoxic effect of the pine needle extracts against cancer cell lines including human gastric carcinoma (KATOIII), human lung carcinoma (A549), human hepatocellular carcinoma (Hep3B) and human breast adenocarcinoma (MCF-7) using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) and SRB (sulforhodamine B) method. The extracts were prepared by step-wise fractionation of ethanol extract of pine needles using diethylether, chloroform, ethylacetate, butanol and water. The growth of the cancer cells in medium containing pine needle extracts were significantly inhibited degree in proportion to the increase of the extract concentration. A significant shrinkage of Hep3B cells was observed when the cells were exposed into 0.5, 1 mg/mL of pinus rigida extract.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.30 no.3
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• pp.1-11
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• 2012

This study examines the implementation process of landscaping projects in Japanese colonial era focusing on landscaping of Keijo Imperial University and draws conclusions as follows. First, landscaping of Keijo Imperial University in Japanese colonial era progressed based on Hoegyebeop and it provides important materials including the floor plan of the garden, detailed statement and other documents required for the construction. Second, it was all Japanese enterprises that took part in landscaping of Keijo Imperial University and the construction progressed under a private contract by lowest-price contract-awarding system after five enterprises' submitting estimates. Third, according to the floor plan for the landscaping, the garden had a shape of a quadrant and its boundaries were utilized as side gutters and connected to a drainage system. Fourth, though the floor plan doesn't clarify specific names of trees, detailed statement shows sorts of trees of the time. Trees of Chosun were plentifully introduced due to regional features and pines were the most expensive one when they were voluminous. This study has significance that it conducts an empirical research on main materials and techniques of landscaping projects in Japanese colonial era.

• Korean Journal of Heritage: History & Science
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• v.47 no.1
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• pp.152-163
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• 2014

In this study, Gwanghanru Pavilion (Namwon-si, Cheonbuk Province) was dated by tree rings of pines (Pinus densiflora Sieb. et Zucc., 'sonamu' in Korean). The present Gwanghanru Pavilion is composed of three parts: main, wing and entrance parts. The main part is known to have been built in 1626 and the entrance hall in 1879, but the date of construction of the wing part is still unknown. We dated a total of 55 wood samples which were replaced during the repair process in 2001~2002. Tree-ring dates indicated that both main and wing parts were built in 1626. Tree rings also revealed that the entrance hall was constructed in 1880~1881, and both main and wing parts were heavily repaired together while appending the entrance hall. In conclusion, dendrochronology, i.e., tree-ring dating, was a useful and accurate method to identify the critical dates-such as the dates of original construction, reconstruction, repairs and extensions-for the history of Korean traditional buildings.

• Journal of the Korean Applied Science and Technology
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• v.40 no.1
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• pp.103-112
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• 2023

This study selected 9 species of domestic native plants (Baechohyang, Red Pine, Turmeric, Ginger, San parsley seed, Bulsugam, Hwangchil, Tangja, Valerian) that have been proven effective for depression, and based on the analysis results of each fragrance component, 9 species were selected. Antioxidant (DPPH, ABTS), cytotoxicity (MTS), and anti-inflammatory (Nitric oxide) experiments were performed by combining essential oils. As a result of the analysis of fragrance components, DL-Limonene (38.44%), g-Terpinene (8.9%), Estragole (5.18%), and a-Pinene (1.73%), which were previously studied in combination oil, were identified. DPPH radical scavenging ability, which is an antioxidant activity, showed 75.4%, ABTS radical scavenging ability was 74.04%, and NO production inhibition was 33.14% at a concentration of 5uL/ml with no cytotoxicity confirmed. Through this, the effect of domestic blending essential oils on the improvement or prevention of depression is verified, and scientific efficacy and ingredient studies are conducted in a mutually cooperative manner to seek solutions to depression and provide basic data to confirm whether or not depressive symptoms are improved. want to do.

• Journal of Life Science
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• v.20 no.4
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• pp.607-613
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• 2010

Osteoporosis is a disease involving a decrease in bone mineral density and an increased risk of fractures. The MC3T3-E1 pre-osteoblastic cell line is a well-accepted model of osteogenesis in vitro. Pine needles have long been used as a traditional health-promoting medicinal food in Korea. In this study, MTT assay, the alkaline phosphatase (ALP) activity and collagen synthesis of osteoblast cells were investigated to determine the effects of pine needle extracts on cell proliferation and differentiation. Pine needle extracts were prepared using hexane, ethanol and water. The effects of the pine needle extracts were examined by comparing the results with those of commercial agents, such as proanthocyanidin. The MC3T3-E1 cells exposed to proanthocyanidin showed increased proliferation in a concentration-dependent manner. The cells exposed to the hexane extract showed a similar increase in proliferation to that observed with proanthocyanidin. The hexane extract showed the highest ALP activity. Moreover, a supplement of pine needle extracts induced collagen synthesis in MC3T3-E1 cells. The pine needle extract produced the highest level of collagen synthesis at concentrations of $10{\sim}50\;{\mu}g/ml$. These results indicate that pine needle extracts have an anabolic effect on bone by promoting osteoblastic differentiation, and may be used in the treatment of common metabolic bone diseases.