• Title/Summary/Keyword: 재처리 기간

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Comparative Study of DNA Fragment According to Steps of Genetically Modified Soybean Processed Food (대두 가공식품의 공정단계별 유전자재조합체(GMO) 단편의 검출확인 및 비교 연구)

  • Hwang, Sun-Wook;Lee, Cheol-Su;Nam, Young-Suk;Kim, Su-Bok;Oh, Duk-Hwan;Kim, Young-Chan
    • Journal of Food Hygiene and Safety
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    • v.18 no.4
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    • pp.211-217
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    • 2003
  • To discriminate between the genetically modified soybean processed foods which were Korean traditional foods as Beansprout, doenjang, Beancurd (Tofu) and the unmodified one. we had analyzed comparatively that the loss degree of inner DNA about denaturalization factors in process step as heat or pressure and decision of suitable PCR primer by size. As a result of having compared about ${\beta}$-actin was 160bp, 335 promotor was 130 bp and NOS terminator was 132 bp effective. As a result of having checked a loss degree of a gene, as for the bean curd, DNA was mostly preserved well, and the loss of DNA along the processing process was hardly observed by a processing process. Most DNA of beansprout have moved to trunk after germination stage, and the appropriate analysis part was judged as the trunk. And the doenjang showed a detection difference of DNA by an operation of an enzye among self-life periods. Besides, after 50days, insertion gene was destoryed entrely so that detection was not possible.

A study on the excavation cycle by the drill-and-blast method for a room-and-pillar underground structure (주방식 지하구조물의 발파 굴착공정 분석 연구)

  • Lee, Chul-Ho;Hyun, Young-Hwan;Hwang, Je-Don;Choi, Soon-Wook;Kang, Tae-Ho;Chang, Soo-Ho
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.18 no.6
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    • pp.511-524
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    • 2016
  • Since a room-and-pillar underground structure is characterized by its grid-type array of room and pillar, its economical efficiency can be governed by excavation sequence. In this study, the construction period by the drill-and-blast method as a excavation method for a room-and-pillar underground structure was examined. In addition, the parallel excavation sequence was considered as the main sequence of a room-and-pillar underground structure. Sequences of mucking and support installation were derived to estimate the total excavation cycle by taking the case of a road tunnel into consideration. From the excavation cycle of room-and-pillar underground structure, the relationship between available maximum and minimum numbers of jumbo drill machines depending on the number of faces in operation was suggested.

무순 추출물의 생리활성 효과

  • 한진희;문혜경;김종국;김귀영;강우원
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 2003.04a
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    • pp.98-98
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    • 2003
  • 무순에는 비타민 C가 많이 들어 있어 겨울철 비타민 공급원뿐만 아니라 디아스타제라는 효소가 들어 있어 소화를 촉진시키는 역할을 한다. 그 외에도 거담제 및 건위제 작용을 하고 음주로 인한 토혈해소, 천식에도 좋아 약용하기도 한다. 본 연구에서는 이용가치는 적지만 농가 소득증대에 기여 할 수 있으며 소화를 촉진시키는 무순, 또는 무싹기름이라고 일컬어지는 무순을 추출용매에 따라 생리활성 효과 분석하고 영양학적 가치가 가장 높은 시기의 무순을 선택함으로써 올바른 섭취의 기초자료를 마련하고 그 기능성을 확인하여 기능성 식품소재 및 기능성 화장품 소재로써의 활용을 검토하고자 하고자 한다. 무순을 4일, 8일, 12일에 따라 incubator에 배양하여 시기별로 채취하여 동결건조 한 후 70% Ethanol, 80% Methanol, 75% acetone, 열수로 환류 추출한 후 시료로 사용하였다. 각 용매 추출물에 대해 DPPH free radical 소거능 실험에서는 acetone 추출물에서 89.18%로 가장 높은 전자공여능을 나타냈으며 각각의 추출용매에서 성장 4일과 12일의 무순에서 높은 전자공여능을 보였다. 아질산염 소거능에서는 pH 1.2의 조건에서 가장 높은 아질산염 소거능을 보였고, 열수 추출물에서 89.70%로 가장 높은 소거능을 보였다. pH 4.2조건에서는 열수추출물의 소거능이 가장 좋았고, pH 6.0 조건에서는 가장 낮은 소거능을 보였으며, Ethanol 과 Methanol 추출물에서 23.55∼37.41%의 소거능을 보였다. SOD유사활성은 성장 8일에서 모두 낮은 활성을 보였으며, 성장 4일과 성장 12일의 무순에서는 큰 차이를 보이지 않았지만, Methanol 추출물중 성장 12일에서 27.41%의 SOD유사활성을 보였다.ic acid는 28.8∼51.7 mg%, 미강에서 321.4∼438.4 mg% 범위로 나타났다. 현미, 백미 및 미강에 함유된 총 폴리페놀의 함량을 표준 페놀화합물로 카테친을 사용하고 비색법에 의하여 측정하였을 때 오대 현미의 폴리페놀 함량은 78.4 mg%, 남평 현미 88.8 mg% 였다. 도정한 백미 중의 총 폴리페놀 함량은 30.3∼56.9 mg%, 미강이 541.5∼472.6 mg%의 범위였다. 이상과 같이 쌀에는 phenolic acid 및 총 폴리페놀이 상당량 함유되어 있으며 특히 배유보다는 강층에 많이 존재하므로 이들 성분의 효율적인 이용을 위한 쌀의 섭취방안이 필요한 것으로 나타났다. 유의적인 상관관계를 나타내고 있어 백편의 조직감은 Compression force 와 Work ratio로 대치할 수 있을 것이라고 사료된다. 수분함량은 기계적 검사보다 관능검사와 더욱 높은 상관관계를 나타냈다.내었다. 항균활성이 우수한 생약재를 농도별로 활성을 조사한 결과, 물 추출물과 10% Ethanol 추출물 모두 낮은 농도에서도 우수한 항균활성을 나타내었다.취와 함께 점질성 갈변물질이 생성되었다. 이와 같은 결과로 볼 때, BAAG의 처리는 BAAC의 경우보다 가격은 저렴하면서도 항균력은 우수한 천연 항균복합제재로써 농산물 식품원료에 적용하여 선도유지 기간을 연장할 수 있는 효과를 기대할 수 있었다. 과일 등의 포장제로서 이용할 가능성을 확인하였다.로 [-wh] 겹의문사는 복수 의미를 지닐 수 없 다. 그러면 단수 의미는 어떻게 생성되는가\ulcorner 본 논문에서는 표면적 형태에도 불구하고 [-wh]의미의 겹의문사는 병렬적 관계의 합성어가 아니라 내부구조를 지니지 않은 단순한 단어(minimal $X^{0}$ elem

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Comparison of Quality of Peeled Lotus Roots Stored in Various Immersion Liquids during Storage (침지액을 달리한 박피 연근의 저장중 품질 비교)

  • Jeong, Jin-Woong;Park, Kee-Jai;Sung, Jung-Min;Kim, Jong-Hoon;Kwon, Ki-Hyun
    • Korean Journal of Food Science and Technology
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    • v.38 no.4
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    • pp.526-533
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    • 2006
  • This study was carried out to investigate the efficacy of strong acidic electrolyzed acid water (SAEW; Strong acidic electrolyzed water, pH 2.76, ORP 1,128 mV, HClO 105.0 ppm) and low alkaline electrolyzed water (LAEW; Low alkaline electrolyzed water, pH 8.56, ORP 660 mV, HClO 73.8 ppm) as storing liquid for peeled lotus root. During storage at $5^{\circ}C$, it was showed that SAEW and LAEW inhibit growth of microorganisms until at least 5 days of storage. Total phenolic contents, polyphenol oxidase (PPO) activity, and color differences value $({\Delta}E)$ of peeled lotus roots stored in SAEW and LAEW were lower than that of one stored in TW (tap water). The hardness decrement of lotus roots stored in SAEW and LAEW were lower than that of lotus root stored in TW too. Contents of moisture crude protein crude fat, crude ash, crude fiber, and total sugars were gradually decreased during storage. Whereas vitamin C content of lotus root stored in 0.6% acetic acid was most rapidly decreased to 25% as compared with one of initial days of storage that of lotus roots stored in SAEW and LAEW was not decreased significantly as compared with one stored in TW. Sensory characteristic during storage was preferable on lotus root stored in SAEW to the other treatments.

Discussion about the Self Disposal Guideline of Medical Radioactive Waste (의료용 방사성폐기물 자체처분 가이드라인에 관한 고찰)

  • Lee, Kyung-Jae;Sul, Jin-Hyung;Lee, In-Won;Park, Young-Jae
    • The Korean Journal of Nuclear Medicine Technology
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    • v.21 no.2
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    • pp.13-27
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    • 2017
  • Purpose In the procedure of domestic medical radioactive self-disposal, there are many requests of supplementation and difficulties on the screening process. In this regard, presentation of basic guideline will improve the work processing efficiency of medical institution radioactive waste. From 2015 to 2016, We reviewed and compared a supplementary requests of domestic fifteen medical institution radioactive self-disposal Plan & Procedure manual. In connection with this, we derive the details of the radioactive waste document based on the relative regulation of nuclear safety Act. The representative supplementary requests of Korea Institute of Nuclear Safety are disposal method of non-flammability radioactive waste, storage method of scheduled self-disposal waste, the legitimacy of self-disposal and pre-treatment of self-disposal, reference radioactivity of disused filter and output of storage period, attachment the evidential matter of measurement efficiency when using a gamma counter. Through establishing a medical radioactive waste guideline, we can clearly suggest a classification standard of radioactive nuclide and the type of occurrence. As a result, we can confirm the reduction of examination processing period while preparing a self-disposal document and there is no spending expenses for business agency. Also, the storage efficiency of facility will better and reduce the economic expenses. On the basis of this guideline, we will expect a contribution to the improvement of work efficiency for officials who has a working-level difficulty of radioactive waste self-disposal.

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Biological Control of Major Pests in Eggplant Greenhouse (시설하우스 가지의 주요해충과 생물적 방제)

  • Paik, Chae-Hoon;Lee, Geon-Hwi;Kim, Doo-Ho;Choi, Man-Young;Kim, Sang-Soo
    • Korean Journal of Organic Agriculture
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    • v.17 no.2
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    • pp.227-236
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    • 2009
  • This research was carried out to investigate the seasonal occurrence of major pests and the effects of releases of natural enemies for biological control of the major pests in eggplant greenhouse. A total of 8 pest species in 7 families were identified. Among these pests, Myzus persicae, Aphis gossypii, Frankliniella occidentalis, Tetranychus urticae and Trialeurodes vaporariorum were the dominant species in eggplant greenhouse. Two aphid species occurred mainly from May to June, but their populations decreased rapidly from July. The population density of F. occidentalis was high from June to July and T. urticae and T. vaporariorum were abundant from July to September during the growing season of eggplant. In the trials of biological control of pests, aphids could be suppressed within the range of $87{\sim}97%$ by two times releases of Chrysopa pallens eggs. F. occidentalis and T. urticae could be suppressed within the range of $76{\sim}90\;and\;87{\sim}91%$ by three times releases of Orius sauteri adults, respectively. The releases of C. pallens eggs and O. sauteri adults were as effective as three times applications of insecticides for the control of aphids, F occidentalis and T. urticae from May to September.

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An Efficient Snapshot Technique for Shared Storage Systems supporting Large Capacity (대용량 공유 스토리지 시스템을 위한 효율적인 스냅샷 기법)

  • 김영호;강동재;박유현;김창수;김명준
    • Journal of KIISE:Databases
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    • v.31 no.2
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    • pp.108-121
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    • 2004
  • In this paper, we propose an enhanced snapshot technique that solves performance degradation when snapshot is initiated for the storage cluster system. However, traditional snapshot technique has some limits adapted to large amount storage shared by multi-hosts in the following aspects. As volume size grows, (1) it deteriorates crucially the performance of write operations due to additional disk access to verify COW is performed. (2) Also it increases excessively the blocking time of write operation performed during the snapshot creation time. (3)Finally, it deteriorates the performance of write operations due to additional disk I/O for mapping block caused by the verification of COW. In this paper, we propose an efficient snapshot technique for large amount storage shared by multi-hosts in SAN Environments. We eliminate the blocking time of write operation caused by freezing while a snapshot creation is performing. Also to improve the performance of write operation when snapshot is taken, we introduce First Allocation Bit(FAB) and Snapshot Status Bit(SSB). It improves performance of write operation by reducing an additional disk access to volume disk for getting snapshot mapping block. We design and implement an efficient snapshot technique, while the snapshot deletion time, improve performance by deallocation of COW data block using SSB of original mapping entry without snapshot mapping entry obtained mapping block read from the shared disk.

Functional Genomics for Mass Analysis of Useful Genes in Panax ginseng C.A. Meyer (인삼의 유용유전자원 확보를 위한 기능 유전체연구)

  • Yang, Deok-Chun
    • Proceedings of the Ginseng society Conference
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    • 2004.05a
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    • pp.17-28
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    • 2004
  • As Korean ginseng is hybrid, an individual variation is very severe, and it takes long times in new breeding because it is required 4 years to pick the seed. But, transformation technique makes the high-functional breeding in short time. The focus of these ginseng studies is to find and secure the useful gene. And it is urgent to accumulate the fundamental data for the molecular breeding and secure the useful genes. Therefore, transformation and soil acclimatization technique are necessary to molecular breeding in use of the introduction of functional genes. In this study, it add to secure of new regulation gene and useful gene as to accumulate the fundamental data for the place where it will contribute to raise the national competitive power. To analyze the useful genes in large scale, we constructed CDNA libraries with various tissues, species, and treated tissue. EST analysis of ginseng perform in large scale and build the EST database of ginseng. We perform the full length sequencing about the selected lots of clones that include the entire open reading frame of the amino acid residues and construct cDNA chip with the parental EST clones. Establishment of the transformation and a soil acclimatization system throuth the re-introduction of the selected ginseng gene that related with the secondary metabolism and anti-stress into the ginseng.

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Effect of manufacturing process using superheated steam on the quality improvement of pickled radish product (과열증기를 이용한 무우절임 제품의 제조공정 설정 및 품질 개선효과)

  • Kim, Eunmi;Lim, Jeong-Ho;Choi, Yun-Sang;Jeon, Ki-Hong
    • Food Science and Preservation
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    • v.24 no.5
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    • pp.600-607
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    • 2017
  • This study was conducted to develop radish as a food product for home meal replacement using superheated steam (SHS). Also, the change of quality characteristics was studied during their storage. The radish cuts were treated with SHS for 0, 3, 5, and 7 min, respectively, followed by complete drying at $80^{\circ}C$ for 6 hours. The results showed that radishes restored with mixed solution (drinking water:sugar:vinegar:salt=2:1:0.8:0.1) were harder than those restored with drinking water. All radishes were stored at 5, 10 and $15^{\circ}C$ for 56 days to investigate the changes of quality characteristics during the storage. Radishes in the control group, restored with drinking water and stored at $15^{\circ}C$, were spoiled after 7 days of storage. The radish in the experimental group did not show any change in the water content; except an increase on the first day of storage. The hardness of radish decreased with an increase in the storage period. It was found that microbial growth was inhibited due to low pH of the mixed solution, in which radishes of the experimental group were immerse.

A Retrospective study of the Cumulative Survival Rate and change of peri-implant marginal bone around implants associated with maxillary sinus augmentation (상악동 골 이식술을 동반하여 식립된 임플란트의 변연골변화와 생존률에 관한 후향적 연구)

  • Ryu, Hwa-Suk;Kim, Sun-Jong;Park, Eun-Jin;Kim, Myung-Rae
    • The Journal of Korean Academy of Prosthodontics
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    • v.47 no.2
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    • pp.240-246
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    • 2009
  • Statement of problem: Insertion of endosseous implants in the atrophic maxilla is often complicated because of lack of supporting bone. Augmentation of the floor of the maxillary sinus with autogenous bone & bone substitute graft has been proven to be a reliable treatment modality, at least in the short term. The aim of this study is to evaluate the factors of implant survival rate associated with maxillary sinus lift with grafts. Material and methods: The sinus floor was augmented with bone grafts derived from modified Caldwell-luctechnique(71 subject, 93 sinus, 180 implants), the autogenous bone or autogenous+Bio-oss. Before implant installation the width and height of the alveolar crest were increased in the first stage procedure in 10 patients while in the other 61 patients augmentation and implant installation could be performed simultaneously width and height of the alveolar crest > 4 mm) or delayed installation. Results: In all case bone volume was sufficients for implant insertion. 14 of 180 inserted implants were lost during follow up and the healing period Patient received implant supported overdenture(5 patients) or fixed bridge(62 patients). Conclusion: Within the limit of the result of this study, we conclude that bone grafting of the floor of the maxillary sinus floor with bone for the insertion of implants might be a reliable treatment modality and the autogenous bone graft and delayed installation method might be the factors for good results.