• Journal of Life Science
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• v.14 no.5
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• pp.821-828
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• 2004

The increasing apparent photosynthetic rate per leaf area may improve seed yield in soybean. Leaf area, length and width are related to the photosynthetic capability of the plant. In this study, two populations derived from the cross of Keunolkong, Shinpaldalkong and Iksanl0 were evaluated with simple sequence repeat (SSR) markers to identify length, width and length/width ratio of leaf. Leaf length/width ratio were significantly negative correlation with leaf width in K/S and K/I populations. In the K/S population, two minor QTLs for leaf length (LL) were found on LG Dlb+W and 1. Two QTLs on LG J and L were related to LL in K/I population. Two and three minor QTLs were identified in leaf width with total phenotypic variation of 13% and 18.04 in K/S and K/I populations, respectively. The leaf length/width ratio, two QTLs on LG I and L, and three QTLs on LG Cl, E and L were related to K/S and K/I populations, respectively. Thus it is assumed that the leaf traits are very much dependent on the genotype used and different breeding approach should be considered for the selection of favorite leaf traits in soybean breeding programs.

• Microbiology and Biotechnology Letters
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• v.35 no.4
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• pp.272-277
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• 2007

The physiological responses of microorganisms to specific nutrient limitation can be regulated at the transcriptional levels. In this study, in order to develop the Pichia pastoris-derived promoter inducible by nutrient-limited condition, we constructed cDNA libraries using RT-PCR of total RNA from P. pastoris in steady-states of phosphate-limited chemostat with different dilution rates. Various genes were detected from cDNA library. Among these genes, the gene encoding putative sodium/phosphate ($Na^+$/Pi) symporter (NPS), high affinity transporter of phosphate, was detected. It was observed that expression of NPS increased in a manner specific to phosphate-limited condition through Northern blot. Therefore, it is thought that the promoter from NPS gene may have the potential as auto-inducible promoter by phosphate-limited culture condition without inducer.

• Journal of Life Science
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• v.31 no.3
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• pp.356-365
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• 2021

Recently, we sequenced the entire genome of a freshwater agar-degrading bacterium Cellvibrio sp. KY-GH-1 (KCTC13629BP) to explore genetic information encoding agarases that hydrolyze agarose into monomers 3,6-anhydro-L-galactose (L-AHG) and D-galactose. The KY-GH-1 strain appeared to possess nine β-agarase genes and two α-neoagarobiose hydrolase (α-NABH) genes in a 77-kb agarase gene cluster. Based on these genetic information, the KY-GH-1 strain-caused agarose degradation into L-AHG and D-galactose was predicted to be initiated by both endolytic GH16 and GH86 β-agarases to generate NAOS (NA4/NA6/NA8), and further processed by exolytic GH50 β-agarases to generate NA2, and then terminated by GH117 α-NABHs which degrade NA2 into L-AHG and D-galactose. More recently, by employing E. coli expression system with pET-30a vector we obtained three recombinant His-tagged GH50 family β-agarases (GH50A, GH50B, and GH50C) derived from Cellvibrio sp. KY-GH-1 to compare their enzymatic properties. GH50A β-agarase turned out to have the highest exolytic β-agarase activity among the three GH50 isozymes, catalyzing efficient NA2 production from the substrate (agarose, NAOS or AOS). Additionally, we determined that GH117A α-NABH, but not GH117B α-NABH, could potently degrade NA2 into L-AHG and D-galactose. Sequentially, we examined the enzymatic characteristics of GH50A β-agarase and GH117A α-NABH, and assessed their efficiency for NA2 production from agarose and for production of L-AHG and D-galactose from NA2, respectively. In this review, we describe the benefits of recombinant GH50A β-agarase and GH117A α-NABH originated from Cellvibrio sp. KY-GH-1, which may be useful for the enzymatic hydrolysis of agarose for mass production of L-AHG and D-galactose.

• Journal of Plant Biotechnology
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• v.50
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• pp.155-162
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• 2023

Radish (Raphanus sativus L.), a root vegetable grown worldwide, is consumed in several ways. In the cross between parental lines to produce F1 seeds of radish, the problem of low purity may arise because of pollen contamination. Therefore, we aimed to establish conditions for callus induction and regeneration so that in vitro cultured plants could be used for the propagation of stock seeds. The most effective hormone combination containing various concentrations of 2,4-D, TDZ, and kinetin was selected for callus induction using radish hypocotyl, and the induced calli were transferred to two types of hormone media to investigate the optimal conditions for shoot regeneration of the callus. The combination of 1 mg/L 2,4-D + 0.05 mg/L kin was the most effective for callus induction of RA2 and RA10, 1 mg/L 2,4-D + 0.1 mg/L kin + 0.025 mg/L TDZ of RA4, and 1 mg/L 2,4-D + 0.2 mg/L kin of RA30. Shoot regeneration of the RA4 callus occurred in both shoot regeneration media, but the frequency was much higher in the 5H+1B medium (1 mg/L NAA + 0.1 mg/L 2,4-D + 1 mg/L IPA + 0.02 mg/L GA3 + 2 mg/L zeatin + 1 mg/L BA). For the in vitro micropropagation of radish, the conditions selected in this study can assist in the propagation and maintenance of stock seeds to produce F1 seeds.

• Journal of Korea Multimedia Society
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• v.7 no.1
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• pp.113-125
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• 2004

Active networks aim to provide a software framework that enables active network applications to customize the processing their communications. Active security component architecture focuses on the support of reuse system by active security component. The architecture is standard layer to acquire, understand, and assemble component, and it has to support a guideline for component identification, search and customization. In this paper we present the active security architecture as a standard model of discrete active network solution, and we propose the method for component classification and specification.

• Proceedings of the Korea Information Processing Society Conference
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• 2014.04a
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• pp.664-667
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• 2014

기존 온톨로지들을 공유 및 재사용하기 위하여 온톨로지 정렬이 연구되고 있다. 기존 정렬 시스템은 온톨로지 데이터 양에 따라 매트릭스를 생성하고 과도한 계산을 통해 처리하여 대용량 데이터 집합에 대하여 공간적 및 계산적으로 부하를 발생하여 효율적이지 않다. 이를 해결하기 위하여 온톨로지 정렬을 휴리스틱 알고리즘을 적용하여 연구 진행하였다. 기존 휴리스틱 알고리즘은 계산이 간단하지만 조율해야 하는 파라미터가 많기에 특정 도메인에 최적 조합이 필요하며 만족한 성능을 얻지 못하였다. 이 논문에서는 Discrete Cuckoo Search(DCS) 기반 온톨로지 정렬 알고리즘을 제안한다. 제안한 알고리즘은 조율해야 하는 파라미터의 개수가 적고 Levy Flight 분포에 따라 탐색하여 계산이 간단하다. 제안된 알고리즘의 성능을 평가하기 위해 OAEI(Ontology Alignment Evaluation Initiative)에서 제공하는 벤치마크 데이터를 사용하여 정확률(Precision)과 재현율(Recall)을 구하고 기존 휴리스틱 정렬 알고리즘과 비교 평가하였다.

• Microbiology and Biotechnology Letters
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• v.33 no.2
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• pp.96-105
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• 2005

For screening of autoregulator receptor gene from Streptomyces longwoodensis, PCR was performed with primers of receptor gene designed on the basis of amino acid sequences of autoregulator receptor proteins with known function. PCR products were subcloned into the BamHIsite of pUC19 and transformed into the E. coli $DH5{\alpha}$. The isolated plasmid from transformant contained the fragment of 100 bp, which was detected on $2\%$ gel after BamHI treatment. The insert, 100 bp PCR product, was confirmed as the expected internal segment of gene encoding autoregulator receptor protein by sequencing. Southern and colony hybridizations with the 100 bp fragment as a probe allowed to select a genomic clone of S. longwoodensis, pSLT harboring a 4.4 kb SphI fragment. Nucleotide sequencing analyses revealed a 651 bp open reading frame(ORF) were isolated protein showing moderate homology ($35{\sim}46\%$) with the ${\Gamma}$-butyrolactone autoregulator receptors from Streptomyces sp., and this ORF was named sltR The sltR/pET-17b plasmid was constructed to overexpress the recombinant SltR protein (rSltR) in E. coli BL21 (DE3)/pLysS, and the rSltR protein was purified to homogeneity by DEAE-Sephacel column chromatography, and DEAE-5PW chromatography (HPLC). The molecular mass of the purified rSltR protein was 55 kDa by HPLC gel-filtration chromatography and 28 kDa by SDS-PAGE, indicating that the rSltR protein is present as a dimer. A binding assay with tritium-labeled autoregulators revealed that the rSltR has clear binding activity with a A-factor type autoregulator as the most effective ligand.

• Microbiology and Biotechnology Letters
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• v.29 no.1
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• pp.18-24
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• 2001

Expression of the baculovirus major envelope glycoprotein gene(gp64) is regulated by transcription from botha early and late promoters. To develop a transient expression vector under the control of gp64 gene promoter, the gp64 gene of Bombyx mori nucleopolyhedrovirus-K1(BmNPV-K1) was characterized. The gp64 gene was local-ized at EcoR I-Pst I 7.38-kb fragment of the BmNPV-K1 genome. The EcorR 1-Pst I 7.38-kb fragment was cloned and the nucleotide sequence of 2,277 bases including the coding region of gp64 gene was determined. Based on these results, transient expression vector using gp64 gene promoter was constructed and named as pBm64. E.coli lacZ gene was introduced onto pBm64 as a reporter gene and expressed transiently in B. mori 5(Bm 5) cells. The expression vector transfected into the cells was maintained stably for 1 to 5 days. In order to confirm the expression of the reporter gene by gp64 promoter, recombinant virus was constructed. The recombinant virus has two independent transcription units in opposite orientations with two promoters; gp64 and polyhedrin gene promoters each initiating transcription of $\beta$-galactosidase and polyhedrin, respectively. Polyhedra formation and expression of $\beta$-galactosidase in Bm5 cells infected with the recombinant virus were observed with phase contrast microscope and in situ staining.

• Journal of KIISE:Computer Systems and Theory
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• v.34 no.8
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• pp.327-336
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• 2007

Lots of effort toward design optimizations have been paid for a cost-effective system design in various ways from a transistor level to RTL designs. In this paper, we propose a bit level optimization of an adder design for expanding its design space. For the bit-level optimization, a heterogeneous adder organization utilizing a mixture of carry propagation schemes is proposed to design a delay-area efficient adder which were not available in an ordinary design space. Then, we develop an optimization method based on Integer Linear Programming to search the expanded design space of the heterogeneous adder. The novelty of the Proposed architecture and optimization method is introducing a bit level reconstruction/recombination of IPs which have same functionality but different speed and area characteristics for producing more find-grained delay-area optimization.

• Journal of the Korea Society of Computer and Information
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• v.10 no.1 s.33
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• pp.201-208
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• 2005

Recently, the container terminal requires the study of method to increase efficiency through change of its operation method. Loading plan is a very important part to increase the efficiency of container terminal. Loading Plan is largely divided into two cases, deciding loading location and loading scheduling and this Paper proposes a more efficient method of container loading scheduling. Container loading scheduling is a problem of combination optimization to consider several items of loading location and operation equipments. etc. An existing method of cluster composition that decides the order of container loading scheduling has a restriction to increase the efficiency of work owing to rehandling problem. Therefore, we Propose a more efficient method of container loading scheduling which composes containers with identical attribution, based on ship loading list and yard map, into stack units of cluster, applying to hierarchical clustering method, and defines the restriction of working order. In this process, we can see a possible working path among clusters by defining the restriction of working order and search efficiency will be increased because of restricted search for working path.