• Microbiology and Biotechnology Letters
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• v.39 no.4
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• pp.364-373
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• 2011

Human embryonic stem cells have been highlighted as a valuable cellular source in the regenerative medicine field, due to their pluripotency. However, there is the challenge of the establishment of specific functional cell type forms of undifferentiated human embryonic stem cells (hESC). To establish and purify functional cell types from hESCs, we differentiated undifferentiated hESCs into vascular lineage cells and sorted the specific cell population from the whole cell population, depending on their cell volume, and compared them with the non-sorted cell population. We observed that about 10% of the PECAM positive population existed in the VEGF induced differentiating human embryoid body (hEB), and differentiated hEBs were made into single cells for cell transplantation. After making single cells, we performed cell sorting using a fluorescence-activated cell sorter (FACs), according to their cell volume on the basis of FSC region gating, and compared their therapeutic capacity with the non-sorted cell population through cell transplantation into hindlimb ischemic disease model mice. 4 Weeks after cell transplantation, the recovery rate of blood perfusion reached 54% and 17% in the FSC regions of sorted cells- and non-sorted cells, respectively. This result suggests that derivation of a functional cell population from hESCs can be performed through cell sorting on the basis of cell volume after preliminary differentiation induction. This approach may then greatly contribute to overcoming the limitations of marker sorting.

• Journal of the Korean Society for Marine Environment & Energy
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• v.16 no.4
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• pp.239-247
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• 2013

Recently renewable energy such as offshore wind energy takes a higher interest due to the depletion of fossil fuel and the environmental pollution. This paper deals with multi-body dynamics (MBD) analysis technique for offshore wind turbine system considering aerodynamic loads and Thevenin equation used for determination of electric generator torque. Dynamic responses of 5MW offshore wind turbine system are evaluated via the MBD analysis, and the system is the horizontal axis wind turbine (HAWT) which generates electricity from the three blades horizontally installed at upwind direction. The aerodynamic loads acting on the blades are computed by AeroDyn code, which is capable of accommodating a generalized dynamic wake using blade element momentum (BEM) theory. In order that the characteristics of dynamic loads and torques on the main joint parts of offshore wind turbine system are simulated similarly such an actual system, flexible body modeling including the actual structural properties are applied for both blade and tower in the multi-body dynamics model.

• The Journal of the Korea Contents Association
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• v.17 no.7
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• pp.546-556
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• 2017

This study aims to find out the key factors that could influence on regional development strategy to use cultural resources. The methods employed are Meta-analysis with 61 case studies in order to draw related factors and Analytic Hierarchy Process with 68 experts in order to analyze the relative importance of important factors. The main findings are as follows: 26 critical factors are drawn from the case studies and they could be classified into four categories, named as 'the capacity building to excavate and utilize cultural resources which are having potential power', 'the establishment of institutional basis for making projects good progress', 'the boosting of internal capability of locals in the process of project', and 'the preparation of cultural governance and multidirectional networking strategies among persons concerned or stakeholder'. The analysis of relative importance of factors shows that among others, 'the capacity building to excavate and utilize cultural resources which are having potential power' is the most fundamental factors to achieve policy goals. There are few studies that have drawn factors from multi-case studies. In this respect, it is far from being complete but meaningful to a little more enhance the generalization of research findings.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.11
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• pp.725-730
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• 2017

The strength ratio of the main structures of buildings gradually increasing, due to the advances made in analysis and cost saving techniques. In this study, to examine the stability of industry buildings using sandwich panels as roof assemblies, we examine the changes in the moment strength ratio of the main structures caused by increasing the roof load. This study adopts the PEB structure and three H-steel structure as the structural analysis models. In the case where the additional load exceeds about 11% of the roof design load, the strength ratio exceeds 1 for the main structure. In the case where the additional load exceeds about 36%(of the roof design load), the working moment exceeds the plastic moments, which leads to major damage to the structure. This study compares 1) the maximum load according to the purlin spaces, 2) the maximum load by KS, and 3) the maximum load calculated from the test results of the manufacturer.The maximum bearing load of the panels determined by all three methods exceeds the structure failure threshold load of the main structure. This study provides evidence that an unexpected increase in the roof load might cause the whole structure to collapse, due to the failure of the main structural members, before the failure of the roof assemblies. Therefore, information on the structural performance of the sandwich panels is required for the structural design, and the sandwich panels should be considered to be an integral part of the overall structural design.

• Journal of KIISE:Databases
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• v.35 no.3
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• pp.286-295
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• 2008

Multimedia consumer electronics(CE) devices, such as MP3 players, PMPs, and digital cameras, are electronic equipments used to record, play or create multimedia data. Most multimedia CE devices provide uses with the ability to search multimedia stored in the device and browse the search results. One of the unique requirements in multimedia CE devices is that the search results displayed in the screen must be restored quickly when the device powers off and later back on. For this purpose, the existing methods (1) re-execute the original search query, and (2) move the cursor to the original position in the search results. However, this approach may be inefficient when the number of records in the result set is large. In this paper, we propose an efficient method for multimedia CE devices that can quickly restore the search results displayed in the screen when the device powers off and later back on. The proposed method can retrieve the original search results in the screen quickly by saving and loading some information about the query evaluation plan. Though the performance evaluation, we show that the proposed method provides excellent performance regardless of the number of records in tile result set or the original cursor position.

• Journal of KIISE:Information Networking
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• v.34 no.6
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• pp.493-504
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• 2007

With the advance and proliferation of computer and wireless network technology, it is common to access to network through the wireless network using mobile device. The ratio of using the streaming media out of many applications through the network is increasing not only in the wired network but also in the wireless network. The streaming media is much bigger than other contents and requires more network bandwidth to communicate and more computing resources to process. However the mobile devices have relatively poor computing resource and low network bandwidth. If the streaming media service is provided for mobile devices without any consideration about the network bandwidth and computing power, it is difficult for the client to get the service of high quality. Since especially mobile devices are supported with very limited energy capacity from the battery, the streaming media service should be adjusted to the varying energy state of mobile devices to ensure the complete playback of streaming media. In this paper, we propose a new method to guarantee the complete playback time of the streaming media for the mobile clients by dynamically controlling transmitted frame rate to the client according to the estimated available time of mobile device using battery model reflecting the characteristic of the battery. Since the proposed method controls the number of frames transmitting to the client according to the energy state of the mobile device, the complete playback time is guaranteed to mobile clients.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.47 no.1
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• pp.83-90
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• 2010

Cancelable biometrics is a robust and secure biometric recognition method using revocable biometric template in order to prevent possible compromisation of the original biometric data. In this paper, we present a new cancelable bits extraction method for the facial data. We use our previous cancelable feature template for the bits extraction. The adopted cancelable template is generated from two different original face feature vectors extracted from two different appearance-based approaches. Each element of feature vectors is re-ordered, and the scrambled features are added. With the added feature, biometric bits string is extracted using helper data based method. In this technique, helper data is generated using statistical property of the added feature vector, which can be easily replaced with straightforward revocation. Because, the helper data only utilizes partial information of the added feature, our proposed method is a more secure method than our previous one. The proposed method utilizes the helper data to reduce feature variance within the same individual and increase the distinctiveness of bit strings of different individuals for good recognition performance. For a security evaluation of our proposed method, a scenario in which the system is compromised by an adversary is also considered. In our experiments, we analyze the proposed method with respect to performance and security using the extended YALEB face database

• The Korean Journal of Ecology
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• v.27 no.2
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• pp.99-106
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• 2004

We developed the estimation model for the vegetation developmental processes on the severely burned slope areas after forest fire in the east coastal region, Korea. And we calculated the vegetation indices as a useful parameter for the development of land management technique in the burned area and suggested the changes of the vegetation indices after forest fire. In order to estimate the woody standing biomass in the burned area, allometric equations of the 17 woody species regenerated by sprouter were investigated. According to the our results, twenty year after forest fire need for the development to the normal forest formed by 4 stratum structure, tree, sub-tree, shrub and herb layer. The height of top vegetation layer, basal area and standing biomass of woody species show a tendency to increase linearly, and the ground vegetation coverage and litter layer show a tendency to increase logarithmically after forest fire. Among vegetation indices, Ive and Ivcd show a tendency to increase logarithmically, and Hcl and Hcdl show a tendency to increase linearly after forest fire. The spatial variation of the most vegetation factors was observed in the developmental stages less than the first 5 years which were estimated secondary disaster by soil erosion after forest fire. Among vegetation indices, Ivc and Ivcd were the good indices for the representation of the spatial heterogeneity in the earlier developmental stages, and Hcl and Hcdl were the useful indices for the long-term estimation of the vegetation development after forest fire.

• Journal of Korea Multimedia Society
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• v.7 no.7
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• pp.896-904
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• 2004

In this paper, We present an efficient variable rate speech coder for spectral efficiency and packet processing technique for packet loss compensation of a voice codec with 10msec frame in VoIP service. Through disconnecting the users from the spectral resource during silence interval of about 60% period, a variable rate voice coder based on a voice activity detection(VAD) can increase spectral gain by two times. The performance of the method was analyzed by variation of detected voice activity factor and degraded speech frame ratio under various background noise level, and compared those of G.729B of ITU-T 8kbps standard speech codec. A method to compensate lost packets utilized addition of recovery data to a main stream and error concealment scheme for speech quality enhancement, the performance is verified by reconstructed speech quality. The proposed scheme can achieve spectral gain by two times or enhance speech quality by 3dB through reserved bandwidth of VAD. Therefore, the proposed method can enhance a spectral efficiency or speech quality of VoIP.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.12
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• pp.521-528
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• 2017

Excessive alcohol consumption is one of the leading causes of many neurological diseases, such as dementia and Alzheimer's disease, and many efforts are under way to solve them. Red ginseng is known to enhance neuronal survival, inhibit apoptosis, and promote nerve regeneration of nerve cells. This study examined whether Rg3-enriched Korean red ginseng extract (KRG) inhibits the apoptosis of PC12 cells caused by alcohol-induced neurotoxicity and how KRG regulates several factors related to the caspase mediated pathway. In this way, the cell survival rate and apoptosis rate of PC12 cells were measured using an EZ-Cytox cell viability assay kit and flow cytometry, respectively. The expression of the apoptosis-related proteins (Bcl-2, Bid, Bax and caspase-3) were analyzed by western blotting, and the significance of the measured results was confirmed using the ANOVA method. As a result, KRG increased the expression of Bcl-2; inhibited the expression of Bid, Bax, and caspase-3; and inhibited the apoptosis of alcohol-induced PC12 cells. These results mean that the KRG-induced increase in Bcl-2 expression and down-regulation of Bid and Bax expression down-regulate caspase-3 expression, which in turn inhibits the mitochondrial apoptotic pathways. This study suggests that KRG is worth developing as a neuroprotective agent candidate.