• Journal of Aquaculture
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• v.10 no.1
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• pp.39-47
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• 1997

Induction of hybrid and allotriploid between female rainbow trout, oncorhynchus mykiss and male cherry salmon, O. masou were performed. Triploid rate in allotriploid was 92% and the survival rates of allotriploid exceeded that of their hybrid counterparts. Karyological studies revealed that the hybrid had exactly the same intermediate diploid karyotypers as that of two parental species, while allotriploid had two sets of the maternal haploid complement and one set of the paternal haploid complement. Hybrid revealed an intermediate size in celluar and nuclear size of erythrocyte, while allotriploid revealed larger cellular and nuclear size of erythrocytes than hybrid. The number of nucleolar organizer regions of hybrid were 1 or 2, while those of allotriploids were 1, 2 or 3. These results proved that allotriploidization can lead to increase cytogenetical characters compared to counterpart hybridization.

• Development and Reproduction
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• v.4 no.1
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• pp.19-28
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• 2000

Diploid and triploid hybrid between female mud loach Misgurnus mizolepis and male cyprinid loach, M. andguillicaudatus were produced, using artificial propagation. The influence of hybridization and triploidization on characteristics of the some tissues of retina optic tectum and trunk kidney in loach was determined histologically. Diploid hybrid have almost size similarities in nucleus and the cell size of some tissues to both parents. In the triploid hybrid the nucleus and／or the cell size in erythrocyte, horizontal cell in inner nuclear layer of retina ganglion cell in ganglion cell layer of retina, neuronal cell in granular layer of optic tectum, and secondary proximal tubule cell of trunk kidney, are much larger than those of the corresponding cells in the diploid hybrid individuals. However, triploid hybrid showed fewer number of cells in outer nuclear layer in retina and nuclei in secondary proximal tubule of trunk kidney than in diploid hybrid. Results of this study suggest that some characteristics in the triploid hybrid exhibiting larger cell and nucleus size with fewer number of cell than diploid hybrid can be useful criteria for the distinction between diploid hybrid and triploid hybrid, and the ploidy level in loach.

• The Korea Swine Journal
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• v.6 no.10 s.62
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• pp.38-43
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• 1984

• Korean Journal of Ichthyology
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• v.29 no.1
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• pp.1-12
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• 2017

A natural hybrid of interspecific between the Coreoleuciscus splendidus and C. aeruginos (Cypriniformes: Cyprinidae) was captured in the Hwang-ji Stream, a tributary of the Nakdong River basin in Korea. An interspecific hybrid between C. splendidus and C. aeruginos was genetically identified based on morphological characteristics and the sequence analysis of nuclear recombination activating gene 1 (RAG1) gene (1,334 bp) and mitochondrial cytochrome c oxidase subunit 1 (CO1) gene (1,551 bp). As a result of morphological variations, the natural hybrid appeared to have an intermediate character between two parental species (C. splendidus and C. aeruginos) in three variations of black array (s) on dorsal, caudal and anal fin rays. Phylogenetic analysis inferred from RAG1 and CO1 sequence data revealed that Coreoleuciscus populations from Hwang-ji stream consist of two pure Coreoleuciscus species and a hybrid individual group. The individuals were clearly identified the cross and reciprocal hybrid by CO1 gene analysis. In RAG1 gene, 13 nucleotide variation loci were detected and the hybrid individuals displayed the double peaks of sequence chromatograms at the 9 diagnostic positions. In this study, molecular data and morphological variations were clearly demonstrated that hybridization did occur between C. splendidus and C. aeruginos. However, F2 hybrid generation and reproductive capacity of F1 hybrid individuals were not demonstrated.

• Korean Journal of Plant Taxonomy
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• v.52 no.2
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• pp.85-96
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• 2022

The plant "Hong-do-go-deul-ppae-gi" has been considered as Crepidiastrum × muratagenii, a hybrid between C. denticulatum and C. lanceolatum, based on its morphological traits and geographical distribution. To reveal the hybrid origin of Hong-do-go-deul-ppae-gi, we examined additional morphological traits of this plant and its putative parents (C. denticulatum, C. lanceolatum, C. platyphyllum) and analyzed one nuclear ribosomal internal transcribed spacer (ITS) region and four chloroplast regions (trnT-L, trnL-F, rpl16 intron, and rps16 intron). As a result of examining the morphological traits, putative hybrid individuals were classified into three types based on the habit, cauline leaf, outer phyllary, and achene beak traits. A molecular analysis found that the ITS sequences of Type 1 and Type 2 individuals showed additive species-specific sites of C. denticulatum and C. lanceolatum. Plastid sequences of Type 1 and Type 2 individuals showed C. denticulatum and C. lanceolatum sequences, respectively. However, Type 3 individuals had ITS and plastid sequences corresponding to C. denticulatum. Accordingly, Type 1 and Type 2 individuals not only share morphological traits with C. denticulatum and C. lanceolatum but also show additive species-specific sites for C. denticulatum and C. lanceolatum, and not C. platyphyllum, supporting its origin as a hybrid between C. denticulatum and C. lanceolatum. Type 3 had morphological traits similar to other hybrid types but was distinguished with respect to outer phyllaries and demonstrated some resemblance to C. denticulatum. In a molecular analysis, Type 3 was found to be identical with regard to the sequence of C. denticulatum and was judged to be an ecological variation of C. denticulatum.

• Korean Journal of Plant Taxonomy
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• v.53 no.2
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• pp.126-147
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• 2023

The taxonomic identity of Elaeagnus ×maritima and E. ×submacrophylla (Elaeagnaceae) in Korea is unclear, yet they are presumed to be hybrid taxa based on their morphology. To determine their hybrid origins, a morphological analysis (field surveys and specimen examinations) and a molecular analysis involving two nuclear ribosomal DNA (nrDNA) regions (internal transcribed spacer and 5S non-transcribed spacer) and one chloroplast DNA (cpDNA) region (matK) were conducted. The morphological analysis revealed that E. ×maritima showed certain morphological similarities to E. glabra, whereas E. ×submacrophylla showed certain morphological similarities to E. pungens. However, the molecular analysis indicated that E. ×maritima exhibited additive species-specific sites of E. glabra and E. macrophylla in the nrDNA regions. Notably, E. ×submacrophylla showed various aspects, with some individuals exhibiting additive species-specific sites of E. pungens and E. macrophylla in the nrDNA and E. macrophylla sequences in the cpDNA regions, some individuals exhibiting E. macrophylla sequences in the nrDNA and E. pungens sequences in the cpDNA regions, and some individuals displaying E. macrophylla sequences in both the nrDNA and cpDNA regions, despite an intermediate morphology between E. pungens and E. macrophylla. These results indicate that these two species are of hybrid origin and frequently cross between parental and hybrid individuals.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.3
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• pp.70-73
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• 1992

사료작물 저온발아성(低溫發芽性)은 수수 $\times$ 수단그라스잡종들이 좋았고 옥수수와 수단그라스는 비교적 저조하였고 PGE에 종자침지처리가 이탈리안라이그라스의 저온발아에 효과적이었다. 간척지의 수수파종시 염해 방지에는 모래를 파종후 피복하여 제염(除鹽) 효과를 얻을수 있었다. 수수류의 토양수분조건에 대한 광합성 연구는 포장용수량의 $60\sim80%$의 조건에서 광합성이 가장 왕성하였고 건물수량도 동일한 결과를 나타내었다. 옥수수의 광이용과 초형과의 관계연구는 이삭상부 엽각의 직립화는 밀식 조건에서 광이용성이 증가되었고 건물 및 종실수량에서 종수가 있었다. 또 밀식화에 따라 직립화 경향이 현저하였으며 옥수수의 다수성초형의 육성이 요청되었다. 질소시비에 대한 청예류의 생장 반응 연구들은 수단그라스에서 10a당 40Kg까지는 건물수량생산을 증가시켰으나 80Kg/10a 사용시는 질산태질소함량이 중독위험치를 초과하였다. 이러한 질산태질소의 중독위험은 6엽기까지 존재하였고 청산중독의 위험농도는 4엽기 이전까지, 또 18/$8^{\circ}C$의 저온조건에서 나타났다. 옥수수의 적정재식 밀도는 10a당 16.600본까지 밀식할수록 증가되었으나 가소화수량과 종실수량은 5.500본과 10Kg/10a 에서 최고치를 나타내었고 질소 추비 효과는 대관령 같은 고산지대에서는 나타나지 않았다. 맥류의 총체(總體)사료 이용성은 호밀, 밀, 보리 순(順)으로 저하하였고 파종시기가 빠를수록 월동성이 향상되었으며 수확적기는 출수후 24일경이었다. 호밀의 청예이용에는 월동후 2회예취와 12cm높이가 건물수량과 TDN수량을 높힐 수 있었다. 수수$\times$ 수단잡종의 출수기 이전 예취는 고사 경비율을 줄일 수 있었고 년 2회 예취와 예취높이 10cm가 가장 좋았다. 유채는 생육기간이 길어질수록 수량은 증가하였고 소화율의 감소도 현저하지 않았다.

• Journal of Practical Agriculture & Fisheries Research
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• v.23 no.2
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• pp.25-32
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• 2021

There is a need to breed and distribute of domestic varieties for Lilium longiflorum because most of the lily bulbs cultivated in Korea were imported from foreign country. The L. longiflorum 'Ace' and 'Nellie White' collected from 1996 had been self-crossed from 1996 to 2010. In 2012, the L. longiflorum 'Bright LoSong' was developed as a F₁ hybrid crossed between 'L₂-17-4' and 'L₂-16-6' that obtained by 7th self-fertilization. The 'Bright LoSong' was tested for homogeneity, regional adaptability test, consumer preference show from 2014 to 2015, and it was registered in 2017. The flower of 'Bright LoSong' is a trumpet shaped single flower with no spots.It has 5.8 flowers per plant, which is a relatively many number compared control 'Mount Carmel', Its flower diamater is 9.8cm, relatively a bit small size. And the flower inflorescence has umbel or raceme while the attitude of longitudinal axis is horizontal. The plant height is 38.4cm with 30.8 leaves per plant and 1.9cm of the leaf width. 'Bright LoSong' has a longer flowering period because of relatively small diameter or many number of flowers than those of the control 'Mount Carmel'. Thus, the 'Bright LoSong' showed a high consumer preference, and the cultivar has the advantage of being able to reproduce since the parents are pure lines.

• FLOWER RESEARCH JOURNAL
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• v.16 no.4
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• pp.304-308
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• 2008

Lilium longiflorum 'Charm' as a $F_1$ hybrid cultivar was released by crossing inbred line '$L_2$-14' and '$L_2$-21' which were obtained from 5 self crosses originated from 'Nellie White', 'Ace' and 'Hinomoto'. The growth and flowering characteristics were evaluated in a greenhouse maintained at a minimum of $13^{\circ}C$ at night during winter in 2006 and 2007. The flower of 'Charm' is white color and horizontal-facing. The flower number of a plant and its diameter is 7.4 and 16.5 cm with 24.5 ornamental(flowering) days. The plant height is 60.3 cm with 70.3 number of leaves. The stem diameter and internode length is 1.2 cm and 1.1 cm, respectively, meaning the plant is compact and sturdy. And the number of seed per a capsule is 251.1. The results of these evaluation, therefore, suggest that seedling Lilium longiflorum 'Charm' can be used as a pot plant due to its short stems, many number of flowers per plant, long ornamental period, strong growth habit with many leaves and thick stem diameter.

• Proceedings of the Botanical Society of Korea Conference
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• 1995.07a
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• pp.109-130
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• 1995

지난 반세기간에 우리나라에 채소 육종은 주요 채소의 주년공급을 가능하게 하였으며, 토지 생산성의 향상, 상품화율의 증대, 품질의 향상 등의 면에서도 괄목할 만한 성과를 거두었고 인공교잡은 물론이고 웅성불임성과 자가불화합성의 활용에 의한 1대잡종 품종의 일반화로 채소 산업의 발전에 크게 기여하였다. 앞으로는 기왕의 업적을 심화시키는 한편, 생산비를 절감하기 위한 생력화, 기계화 재배용 품종 및 내제초제성 품종의 개발 환경보호 및 식품안정성의 확보를 위한 내병층성 품종 개발, 수출시장과 다양화하는 국내의 시장기호에 대응하고 가공 식품의 표준화된 품질관리를 지원할 수 있도록 품질 면에서의 개량과 신작물 또는 신생태형 품종의 개발에도 더욱 노력이 필요하다. 이러한 육종목표를 달성하기 위한 유전자원의 확보는 더욱 어려워질 것이며 유전 양식이 복잡하고, 환경요인의 작용이 상대적으로 크기 때문에 전통적인 육종 방법만으로는 목표달성에 필요한 인적, 물적, 시간적 소요가 훨씬 증가될 전망이다. 유전변이의 창성 및 확대, 유용 대립인자의 도입, 동정 및 선발, 그리고 종자생산을 위한 자가 불화합성 및 웅성불임성과 개화·수정 관련 유전인자의 발현 조절에 분자유전학의 보완적 역할이 기대되며 이렇게 되면 전통육종과 분자유전학간의 잡종강세로 품종 개량의 효율은 크게 높아질 것이다.