• Microbiology and Biotechnology Letters
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• v.49 no.1
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• pp.101-110
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• 2021

Interactions between microalgae and heterotrophic bacteria are common in natural environments. This study investigated the effect of heterotrophic bacteria on the activity of the photosynthetic eukaryotic alga Chlorella sp. MF1907 when cocultured. A total of 31 heterotrophic bacterial isolates belonging to different genera were cocultured with MF1907. Interactions of the alga with Agromyces, Rhodococcus, Sphingomonas, Hyphomicrobium, Rhizobium, and Pseudomonas were positive, while those with Burkholderia, Paraburkholderia, Micrococcus, Arthrobacter, Mycobacterium, Streptomyces, Pedobacter, Mucilaginibacter, Fictibacillus, Tumebacillus, Sphingopyxis, and Erythrobacter were negative (p < 0.05). A turnover experiment demonstrating a switch from heterotrophic to autotrophic activity of MF1907 was performed using 16 isolates exhibiting apparent effects (positive, negative, or neutral). Compared with the results of the coculture experiment, eight isolates exhibited the same outcomes, while the others did not. Consistently, Pseudomonas and Agromyces showed a remarkable positive effect on MF1907 activity, and Burkholderia, Streptomyces, and Erythrobacter had a marked negative effect. Our results suggest that it may be possible to use the isolates for controlling populations of microalgae in natural and engineered environments.

• Korean Journal of Environment and Ecology
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• v.35 no.3
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• pp.237-255
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• 2021

Lychnis wilfordii (Regel) Maxim. is one of the wetland specialists mainly distributed in peatlands at high latitudes. In Korea, it is isolated in two regions. This study investigated habitats, growth traits, and self-compatibility of L. wilfordii and assessed the internal dynamics of its population persistence. Its population has remained stable in the Yongneup Wetland Protected Area (YWPA). There was a clear difference in vegetation environment between YWPA and the distribution area in Daegwallyeong (DWL), Pyeongchang-gun. It has self-compatibility while pollinators facilitate its seed production. It produces a large number of hibernacles and bears the maximum number of branches and fruits in soil with rich organic contents. However, it grows and bears fruits even under the condition of low organic contents. In YWPA, L. wilfordii is not distributed in high moor but widely distributed in low moor where tussocks by Carex thunbergii var. appendiculata are developed. It is mainly distributed on the top of tussocks also. Therefore, it is judged that the formation, growth, and extinction of tussocks by C. thunbergii var. appendiculata is closely related to the establishment, growth, and extinction of plants distributed in this space. It is assessed that the current YWPA has well-developed tussocks in which L. wilfordii is widely distributed, and extinction and re-establishment progress well. Accordingly, the L. wilfordii population is expected to be sustainable in the long term given if its current ecological process is maintained well.

• Journal of The Korean Society of Grassland and Forage Science
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• v.40 no.4
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• pp.244-250
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• 2020

This study investigated the effects of silage additives on rumen fermentation characteristics of rye silage. Rye was harvested at ripening stage and treated with different additives in quadruplicate following: without additive (control), with either lactic acid bacteria inoculant (LAB), formic acid (FA), or Ca-formate (Ca-FA). Overall, ensiling characteristics of FA and Ca-FA silages contained 4-fold more (P<0.05) butyrate and 2-fold more (P<0.05) NH3-N concentration (% total nitrogen) than those of control and LAB silages. Cows fed LAB silage showed a diurnal trend with the highest values of propionate concentration compared to the control at 1, 2 and 3 hr after feeding. In contrast, FA and Ca-FA silages increased the proportion of butyrate significantly (P<0.05) at all sampling times compared to control and LAB silage. In conclusion, Forage rye treated with FA or Ca-FA showed different fermentation characteristics during ensilage and in the rumen compared to LAB silage. Further studies are needed to evaluate whether different fermentation characteristics in the rumen between LAB and FA silages had effect on partitioning of nutrients between milk production and body tissue.

• Journal of the Korean earth science society
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• v.42 no.6
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• pp.717-733
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• 2021

This study is aimed to provide basic data to set the direction of polar literacy education and to raise awareness of the importance of polar research. Elementary, middle, and high school students' perception of the polar region was examined in terms of current status of polar information, impression regarding polar regions, and awareness of related issues. The study included 975 students from nine elementary, middle, and high schools, who responded to 16 questions, including close-ended and open-ended items. The results suggest that students had more experiences regarding the polar region on audiovisual media, but relatively limited learning experiences in school education. The impression they had of the polar region was confined to the monotonous image of a polar bear in crisis, following the melting of the glacier due to global warming. The students formed powerful images by combining scenes they saw in audiovisual media with emotions. In terms of recognizing problems in the polar region, the students were generally interested in creatures, natural environment, and climate change, but their interests varied depending on their school level and their own career path. The students highly valued the scientist's status as agents to address the problems facing the region, and gave priority to global citizenship values rather than practical standards. Based on the results, we suggest the following: introducing and systematizing content focusing on the polar region in the school curriculum, providing a differentiated learning experience through cooperation between scientists and educators, establishing polar literacy based on concepts that are relevant to various subjects, earth system-centered learning approach, setting the direction for follow-up studies and the need for science education that incorporates diverse values.

• Korean Journal of Poultry Science
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• v.49 no.2
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• pp.99-108
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• 2022

Avian leukosis virus (ALV) is a highly contagious retrovirus that causes tumors and has resulted in great economic loss worldwide owing to its high transmission rate. Various ALV viral subgroups exist, with infections occurring via specific host receptors. The susceptibility or resistance of avian species to the ALV-A and K subgroups is determined by the host receptor, the tumor virus locus A (tva) gene, while that to ALV-B depends on another host receptor, the tumor virus locus B (tvb) gene. The resistance alleles of tva and tvb have primarily been identified in China, but none have beendetected in Korea. We analyzed the frequencies of tva and tvb genotypes in White Leghorn (WL), Korean Ogye (KO), and Korean native chicken (KNC) breeds, and assessed the resistance to ALV subgroups. In WL, both tva and tvb had various genotypes, including susceptibility and resistance alleles, whereas in KO, tva and tvb resistance alleles were dominant. In KNC, tva susceptibility and resistance alleles were mixed, whereas tvb resistance alleles were dominant. In addition, we showed that there were differences in the splicing pattern of tva transcripts and the expression level of tvb transcripts within breeds. Finally, we confirmed that ALV resistance depended on KO and KNC genotypes by in vitro infection of chicken embryonic fibroblasts with ALV. These results highlight that some KO and KNC individuals are naturally resistant to ALV subgroups A, B, and K, and will facilitate the preservation of economically superior traits through selective breeding.

• Trans-
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• v.12
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• pp.141-171
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• 2022

This study was conducted to understand media and cultivate personality by using media as data for personality education. To achieve this purpose, the Personality Education Promotion Act and the Korea Educational Development Institute's personality virtues were selected as educational elements, and a personality education program using media was developed in combination with the middle school curriculum. For this study, first, in order to extract personality virtues, 13 personality virtues were finally selected as educational elements by comparing and synthesizing the personality virtues of the Personality Education Promotion Act and the Korea Education Development Institute. The final personality virtues selected are self-esteem, courage, sincerity, self-regulation, wisdom, consideration, communication, courtesy, social responsibility, cooperation, citizenship, justice, and respect for human rights. Second, in order to select media and set the direction of development of personality education programs, the process of collecting media data was confirmed, and the direction and goal of the program were set by analyzing the middle school curriculum. Third, in order to propose a method of applying a personality education program using media, the personality grafting unit was selected by referring to the commentary on all subjects of the 2015 revised curriculum.

• Journal of Korean Home Economics Education Association
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• v.34 no.2
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• pp.129-152
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• 2022

The purpose of this study was to develop a sustainable dietary education program for middle school home economics subject using a teaching strategy to improve the empowerment of adolescents and to verify and evaluate the effectiveness of the program. To achieve the purpose of this study, the program was developed and evaluated according to the ADDIE teaching design model. The contents related to the dietary area were extracted from the technical & home economics curriculum of the 2015 revised middle school and SDGs, and their relevance was analyzed to select the contents of dietary education. The program developed based on the analysis results is 'dietary life together' and consists of five learning topics: 'living together in the global village', 'maintaining healthy diet', 'creating a dietary culture together', 'living with nature and people', and 'maintaining a safe diet'. As a strategy for improving empowerment, we presented four situations, each of which represents value judgment, prediction of results, responsible behavior choice, and decision making. The developed program was reviewed by experts and applied to 17 unit classes for 17 weeks (1 unit hour per week) to the third graders of middle schools in Gyeonggi-do. Significant differences were found between before and after the class measurements of the personal empowerment and the political and social empowerment, which shows the classes were effective in improving empowerment. However, since there was no significant difference in interpersonal empowerment before and after the program, suggestions were made to utilize strategies to facilitate discussion and cooperative learning when implementing the program. The students who participated in the class evaluated the program positively as a whole. The program was evaluated to have helped the students believe they could change society through solving dietary problems.

• Journal of Korean Elementary Science Education
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• v.41 no.1
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• pp.65-78
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• 2022

This study aims to 1) verify the validity of the Science self-efficacy scale and 2) perform a latent mean analysis of the background variables about a pre-service teacher. The study uses pre-tests to analyze data from 187 pre-service teachers, which uses Tark's Science self-efficacy scale (2011). To identify the factor structure, exploratory factor analysis was performed. Based on the results of the pilot test, the expert group council revised the scale for the pre-service teachers to respond to the 3-factor structure. In the main test, 354 data were analyzed through a modified Science self-efficacy scale, and exploratory and confirmatory factor analyses were performed. The results of the study are as follows: First, in the pilot test, the pre-service teacher responded to a 3-factor instrument, but the validity of two items was examined further below. Second, the pre-service teachers responded to a 3-factor instrument on 29 items for the modified Science self-efficacy scale. The total reliability of the instrument was .886 and the reliability of each factor was analyzed as .882-.886. Finally, the latent mean analysis by gender showed that females have a higher self-regulation efficacy factor and males have a higher self-confidence factor (Cohen's d > .3). Furthermore, there is a significant difference in task difficulty preference and self-regulatory efficacy factor (Cohen's d > .8) between the college preparatory and science subject preference. This study provides important insights into and contributions to the accurate scientific self-efficacy diagnosis of pre-service teachers, as well as proposes a curriculum to improve the scientific self-efficacy of prospective teachers.

• Journal of Korean Society of Forest Science
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• v.111 no.4
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• pp.667-675
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• 2022

Agroforestry is an ecological and economic land-use system that enables sustainable agriculture by combining forestry, agriculture, and livestock industries. North Korea chose agroforestry as a strategy for the restoration of sloping land and deforested land. Agroforestry was proposed for the inter-Korean forest cooperation subcommittee meeting and is currently highlighting carbon removal and promoting the '2050 Carbon Neutral Strategy' and 'Korea Peninsula Green Détente.' The study area, Suan-gun, Hwanghaebuk-do, is a constant deforestation monitoring area and a pilot site for management by the International Center for Research in Agroforestry. The requirements for agroforestry were analyzed through literature analysis. The agroforestry site-suitability map was visualized by applying GIS overlap analysis. Approximately 8,839 ha of sloping area was selected as suitable for agroforestry management, which is about 15% of Suan. We compared the map with Google Earth images and visually detected the land use status, such as agroforestry in Suan, to verify the results. As a future study, we will consider both natural-environment and socioeconomic factors and evaluate the relative importance of the factors to produce a high-accuracy agroforestry sitesuitability map at the national scale with the goal of producing basic data for the inter-Korea forest cooperation policy for long-term goals.

• Korean Chemical Engineering Research
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• v.60 no.3
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• pp.398-406
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• 2022

The outer membrane of Gram-negative bacteria is the outermost layer of cellular environment in which numerous biophysical and biochemical processes are in action sustaining viability. Advances in cell engineering enable modification of bacterial genetic information that subsequently alters membrane physiology to adapt bacteria to specific purposes. Surface display of a functional molecule on the outer membranes is one of strategies that directs host cells to respond to a specific extracellular matter or stimulus. While intracellular expression of a functional peptide or protein fused to a membrane-anchoring motif is commonly practiced for surface display, the method is not readily applicable to exogenous or large proteins inexpressible in bacteria. Chemical conjugation at reactive groups naturally occurring on the membrane might be an alternative, but often compromises fitness due to non-specific modification of essential components. Herein, we demonstrated two distinct approaches that enable site-specific decoration of the outer membrane with a fluorescent agent in Escherichia coli. An unnatural amino acid genetically incorporated in a surface-exposed peptide could act as a chemoselective handle for bioorthogonal dye labeling. A surface-displayed α-helical domain originating from a part of a selected heterodimeric coiled-coil complex could recruit and anchor a green fluorescent protein tagged with a complementary α-helical domain to the membrane surface in a site- and hetero-specific manner. These methods hold a promise as on-demand tools to confer new functionalities on the bacterial membranes.