• Title/Summary/Keyword: 자매종

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A new combination for Saxifraga octopetala (Saxifragaceae) and its phylogenetic relationship (구실바위취의 신조합명 및 계통 유연관계)

  • Kim, Yong-In;Cho, Seong Hyun;Kim, Bo-Yun;Lee, Jung-Hoon;Kang, Dae-Hyun;Kim, Soonok;Doudkin, Roman V.;Kim, Young-Dong
    • Korean Journal of Plant Taxonomy
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    • v.45 no.4
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    • pp.306-317
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    • 2015
  • This study was conducted to address the taxonomic status of the Korean endemic species Saxifraga octopetala, which is sometimes considered conspecific to Micranthes manchuriensis. Extensive molecular phylogenetic analysis using nrITS sequences as well as morphological examination of type specimens of the two species were undertaken to ascertain the phylogenetic position and species delimitation of S. octopetala. In the resulting nrITS trees, a total of 65 accessions representing S. octopetala grouped together and nested within the Micranthes clade, exhibiting a close relationship with M. nelsoniana and M. manchuriensis. Multiple accessions of M. manchuriensis collected from China and Russia also formed a clade, showing a sister group relationship with M. nelsoniana var. pacifica and M. fusca. The ambiguous species entity of S. octopetala is thought to have originated from Nakai's misinterpretation of Wilford's collection (type specimens of M. manchuriensis), which is a complex collection including an inflorescence of M. nelsoniana. In spite of apparent morphological similarity between S. octopetala and M. manchuriensis, they differ in the presence and absence of underground stolons. The distinct position of S. octopetala within the Micranthes clade on the nrITS tree suggests that it should retain species status in Micranthes. Thus a new combination (Micranthes octopetala) is proposed.

Phylogenetic Relationships of the Genus Hemerocallis in Korea using rps16-trnK Sequences in Chloroplast DNA (엽록체 rps16-trnK 서열에 의한 한국 내 원추리속 식물종의 계통 관계)

  • Huh, Man Kyu;Kwon, Oh Sung;Lee, Byeong Ryong
    • Journal of Life Science
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    • v.23 no.7
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    • pp.847-853
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    • 2013
  • The genus Hemerocallis (family Xanthorthoeaceae) is a herbaceous species, some of which are very important in herbal medicines. We evaluated the rps16-trnK region of the chloroplast DNA of a representative sample of eight taxa in Korea to estimate phylogenetic relationships within the taxa of this genus. Due to differences in the number of inserted nucleotides, the aligned data for Hemerocallis ranged from 729 (H. aurantiaca) to 742 nucleotides (H. fulva var. kwanso), with a mean of 736. Although several small indels and 20 inserts were present, sequence variation within the Hemerocallis genus was mostly due to nucleotide substitutions. All rps16-trnK trees generated in Korea exhibited a well-solved topology, with high bootstrap support, irrespective of the methods (parsimony) and the setting used. The node of H. minor and H. littorea was strongly supported, with a high bootstrap value in three trees, and these two taxa were sistered with H. thunbergii. The number of chromosomes was not congruent with that found in a previous study with RAPD, but the number was in agreement with the results of this study.

Comparative Study on Effects of Sexuality Education in Elementary School Students (초등학생의 성교육 효과에 관한 비교연구)

  • Seo, Soon-Hee;Hong, Jee-Young;Lee, Moo-Sik;Na, Baeg-Ju;Lee, Jin-Yong;Bae, Seok-Hwan;Yoo, Se-Jong
    • Proceedings of the KAIS Fall Conference
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    • 2010.11b
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    • pp.513-516
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    • 2010
  • 본 연구는 성교육이 학생들의 성 지식, 태도, 역할 정체감에 미치는 효과를 알아보기 위해 실험군과 대조군으로 집단을 나누어 실험군에게 의도적인 성교육 실시하여 그 결과를 살펴보고 향후 학교에서 실시되는 성교육 계획 수립에 도움을 주고자 하는 실험연구이다. 본 연구의 대상은 충청북도 제천시에 위치한 J초등학교 5학년 6개 학급을 대상으로 실험군 100(남 50, 여 50), 대조군 100(남 50, 여 50) 총 200명을 대상으로 실시하였으며 자료 수집은 2009년 10월 15일부터 2009년 11월 25일까지 실시하였다. 연구도구는 문헌고찰을 통해 성 지식, 성 태도, 성역할 정체감에 사용한 설문지를 구성타당도 검증을 통해 문항을 구성 선정하였으며, 신뢰도는 Cronbach's ${\alpha}$ 값으로 검증하였다. 연구 대상자의 일반적 특성을 보면 92%의 학생이 부모님이 있고, 88.5%가 형제자매가 있으며, 집안 분위기는 60%가 자유스럽고, 7%가 엄한 것으로 나타났다. 연구 대상자의 성관련 특성으로 가정에서 성교육을 받아본 경험이 있는 경우는 36%로 나타났으며, 성과 관련된 정보를 얻는 순서는 87.5%의 학생이 학교 보건선생님한테 성 관련 정보를 얻는 경우가 대부분이었고, 다음 순서로는 부모님, 서적류, 담임선생님, 영상매체, 친구, 형제자매 순으로 나타났다. 연구 대상자의 신체변화정도를 살펴보면 여자의 경우 월경이 있는 경우는 20%정도, 가슴의 변화는 84%로 나타났으며, 남자의 경우 몽정이 있는 경우는 4%로 나타났으며, 남녀 모두 변성기, 여드름, 발모, 몽정, 월경, 가슴변화 등 한 가지 이상 신체변화가 나타난 경우는 56%로 나타났다. 연구 대상자의 성지식 사전 사후 점수를 비교해보면 두 집단 모두 사전보다 사후 점수가 증가하였다. 사후검사에서 교육을 받지 않은 대조군의 성지식 점수는 소폭 증가하였으나 성교육을 실시한 실험군의 성지식 점수는 더 큰 폭으로 높았으며, 특히 신체발달에서 사전검사에 대조군은(M=2.17), 실험군은 (M=1.66)으로 실험군의 점수가 낮았으나, 사후검사에서는 대조군은(M=2.76), 실험군은(M=3.90)으로 실험군의 점수가 더 높았다. 이상의 결과를 종합해보면 학교에서의 체계화된 성교육은 학생들의 성 지식, 태도, 성 역할 정체감 형성에 도움을 줄뿐만 아니라 성교육을 부끄러워하고, 쑥스러워하며, 어색해 하던 아이들이 문제 중심 학습을 적용하여 자기 주도적으로 문제를 해결하고, 체험, 실습, 역할극, 토의 등의 활동적인 수업에 적극 참여하고, 자연스럽게 활동하여 긍정적인 '밝은 성(性)'으로 변해가는 모습을 관찰할 수 있었다. 성교육은 인간의 성장과 발달에 따라 변화하는 과정이라는 것을 인식하여 건전한 성, 건강한 성을 함양하기 위해 체계적이고 발달 단계에 맞추어 이루어져야 할 것이다.

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Endless debates on the extant basal-most angiosperm (현생 기저 피자식물에 대한 끝나지 않는 논쟁)

  • Kim, Sangtae
    • Korean Journal of Plant Taxonomy
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    • v.40 no.1
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    • pp.1-15
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    • 2010
  • Recognizing a basal group in a taxon is one of the most important factors involved in understanding the evolutionary history of that group of life. Many botanists have suggested a sister to all other angiosperms to understand the origin and rapid diversification of angiosperms based on morphological and fossil evidence. Recent technical advances in molecular biology and the accumulation of molecular phylogenetic data have provided evidence of the extant basal-most angiosperm which is a sister to all other angiosperms. Although it is still arguable, most plant taxonomists agree that Amborella trichopoda Baill., a species (monotypic genus and monotypic family) distributed in New Caledonia, is a sister to all other extant angiosperms based on evidence from the following molecular approaches: 1) classical phylogenetic analyses based on multiple genes (or DNA regions), 2) analyses of a tree network of duplicated gene families, and 3) gene-structural evidence. As an alternative hypothesis with relatively minor evidence, some researchers have also suggested that Amborella and Nymphaeaceae form a clade that is a sister to all other angiosperms. Debate regarding the basal-most angiosperms is still ongoing and is currently one of the hot issues in plant evolutionary biology. We expect that sequencing of the whole genome of Amborella as an evolutionary model plant and subsequent studies based on this genome sequence will provide information regarding the origin and rapid diversification of angiosperms, which is Darwin's so called abominable mystery.

Molecular phylogeny of Astilbe: Implications for phylogeography and morphological evolution (노루오줌속(Astilbe)의 분자 계통: 계통지리 및 형질 진화에 대한 고찰)

  • Kim, Sang-Yong;Kim, Sung-Hee;Shin, Hyunchur;Kim, Young-Dong
    • Korean Journal of Plant Taxonomy
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    • v.39 no.1
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    • pp.35-41
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    • 2009
  • Astilbe (Saxifragaceae) is a genus well known for its disjunctive distribution in Asia and eastern North America. In this study, we reconstructed a molecular phylogeny of the genus using the sequences of ITS regions of nuclear ribosomal DNA. A total of 17 species representing major lineage of Astilbe and closely related taxa were included in the phylogenetic analyses. We obtained a Bayesian phylogenetic tree in which Saxifragopsis was positioned as a sister group to Astilbe. The Japanese endemic species, A.platyphylla was the most basal lineage within the genus. This species is well known for its distinct morphological features such as unisexual flowers, apetaly, and calyx with 7-11 lobes. Two species, A. biternata, a New World representative of the genus, and A. rivularis widely distributed in S. Asia, branched off early in the evolution of Astilbe. The remaining species formed a strongly supported core clade, which diverged into two robust geographical lineages: the first ("Japonica" clade) of species distributed in Japan, Taiwan, and Philippines and the other ("Rubra" clade), of taxa in China and Korea. The ITS phylogeny indicates that the Bering land bridges were the major route for the origin and dispersal of A. biternata. The two Taiwanese taxa and A. philippinensis were found to derive from the Japanese member, as the genus advanced southwards. The ITS phylogeny suggests that apetaly originated independently at least two times within the genus. Our results do not support Engler's classification system of the genus based on the leaf type (simple vs. compound), but reaffirm Hara's taxonomic idea which primarily considered the features of calyx.

Genetic and Phylogenetic Relationships of Genus Hemerocallis in Korea Using ISSR (ISSR에 의한 한국 내 원추리속 식물의 유전적 및 계통학적 연구)

  • Choi, Joo-Soo;Huh, Hong-Wook;Lee, Seol-A;Huh, Man-Kyu
    • Journal of Life Science
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    • v.18 no.6
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    • pp.753-758
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    • 2008
  • Genus Hemerocallis is a herbaceous species and some species among their taxa are very important herbal medicines. We evaluated representative samples of the eight taxa in Korea with inter simple sequence repeats (ISSR) markers to estimate phylogenetic relationships within taxa of this genus. The studied taxa were Hemerocallis fulva L., H. fulva for. kwanso, H. dumortieri Morren, H. coreana Nakai, H. hongdoensis M.G.Chung & S.S.Kang, H. middendorffi Trautv. et Mayer, H. thunbergii Baker, H. minor Miller. In addition, we investigated the genetic variation and structure of Korean populations of these taxa. The mean genetic diversity was 0.098 across species, varying from 0.068 to 0.123. A low level of genetic variation was found in populations of Hemerocallis species. Specially, gene diversity for H. minor was maintained the highest among genus Hemerocallis. An indirect estimate of the number of migrants per generation (Nm=0.218) indicated that gene flow was not extensive among Korean populations of Hemerocallis species. The phylogenic tree showed distinct three clades. One includes H. fulva, H. fulva for. kwanso and H. middendorffi. Another includes three Hemerocallis species, H. dumortieri, H. thunbergii and H. minor. The H. coreana and H. hongdoensis were shown as the sister group to the second clades. Although the size of sampling was not large enough for eight Korean Hemerocallis species, the analyses of ISSRs will certainly provide an enhanced view on the phylogeny of species.

Phylogenic Study of Genus Citrus and Two Relative Genera in Korea by trnL-trnF Sequence (trnL-trnF 서열에 의한 한국 귤나무속과 두 근연 식물종의 계통분류학적 연구)

  • Huh, Man-Kyu;Yoon, Hye-Jeong;Choi, Joo-Soo
    • Journal of Life Science
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    • v.21 no.10
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    • pp.1452-1459
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    • 2011
  • Citrus is a common term and genus (Citrus) of flowering plants in the rue family, Rutaceae. Citrus is believed to have originated in the part of Southeast Asia bordered by Northeastern India, Myanmar (Burma) and the Yunnan province of China. The taxonomy and systematics of the genus are complex and the precise number of natural species is unclear, as many of the named species are clonally propagated hybrids, and there is genetic evidence that even some wild, true-breeding species are of a hybrid origin. One of the most popular sequences for phylogenetic inference at the generic and infrageneric levels in plants is the chloroplast trnL-trnF region. We evaluated the seven taxa with the trnL-trnF region to estimate phenotypic relationships within the genera Citrus, Poncirus, and Fortunella in Korea. Alignment of the DNA sequences required the addition of numerous gaps. Sequence variation within Citrus was mostly due to insertion/deletion. Within the genus Citrus, C. lomonia and C. sinensis were relatives and sistered with C. aurantium in the four phylogenetic analyses (MP, ML, ME, and NJ). However, some external nodes were poorly supported.

Morphological Characteristics of the Blue Trevally, Carangoides ferdau (Perciformes: Carangidae) and its Phylogenetic Relationships among Korean Relatives (흑전갱이, Carangoides ferdau의 형태적 특징 및 분자계통분류학적 위치)

  • Kim, Joon Sang;Song, Choon Bok
    • Korean Journal of Ichthyology
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    • v.25 no.4
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    • pp.222-226
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    • 2013
  • As Carangoides ferdau was previously reported based on its underwater photograph, morphological descriptions have been incomplete up to the presence in Korea. On the base of two samples collected at the coast of Jeju island, morphological characters of C. ferdau are described in detail. This species is characterized by having the forepart of second dorsal fin much prolonged, 7~8 transverse dark bands on body, and snout length almost equal to eye diameter. It is morphologically very similar to C. orthogroammus, but is easily distinguished in having transverse dark bands instead of yellow spot on the body of C. orthogroammus. Phylogenetic relationships based on the mitochondrial cytochrome b (1,141 base pairs) sequences shows that C. ferdau is closely related to C. orthogroammus, and C. dinema also has a sister group relationship with C. ablongus. Both genetic distances (p-distances) are 8.2%, respectively.

Systematics of Korean Thalictrum L. based on a morphological cladistic analysis (형태학적 분계분석에 의한 한국산 꿩의다리속(Thalictrum L.) 식물의 계통학적 연구)

  • Park, Seongjun;Park, Seon-Joo
    • Korean Journal of Plant Taxonomy
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    • v.39 no.2
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    • pp.89-99
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    • 2009
  • We used Maximum parsimony and Neighbor-joining to investigate phylogenetic relationship of the genus Thalictrum in Korea with 39 morphological characters. The plant materials were used for ingroup 21 taxa and outgroup 1 taxon including a Korean endemic plant. This study confirmed that Sect. Thalictrum and Sect. Camptonotum formed monophyletic group by 100% and 83% bootstrap values respectively, and Sect. Camptonotum appeared to be Sect. Thalictrum's sister group. Sect. Erythrandra and Sect. Physocarpum formed paraphyletic groups. Sect. Physocarpum except T. osmorhizoides appeared to be polytomy. Sect. Tripterium and Sect. Omalophysa formed an independent clade. The shape of stamen is the important character representing the phylogenetic pattern of the genus Thalictrum; the bat and filiform types might be derived from clavate type. The pollination patterns (entomophily, anemophily), and presence of calyx at flowering time may also be related to the evolution of the Thalictrum flowers.

A systematic study of Glechoma L. (Lamiaceae) based on micromorphological characters and nuclear ribosomal ITS sequences (미세구조학적 형질 및 핵 리보솜 DNA의 ITS 염기서열에 의한 긴병꽃풀속(꿀풀과)의 계통분류학적 연구)

  • Jang, Tae-Soo;Lee, Joongku;Hong, Suk-Pyo
    • Korean Journal of Plant Taxonomy
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    • v.44 no.1
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    • pp.22-32
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    • 2014
  • The petal and sepal micromorphology of five species of Glechoma (Lamiaceae) was investigated to evaluate their taxonomic significance, and a molecular phylogeny using the sequences of internal transcribed spacers (ITS) regions of nuclear ribosomal DNA was carried out to resolve their phylogenetic relationships. Stomatal complexes were mostly found in the inner and outer part of the sepal from all investigated taxa, and the size length of the guard cell was variable among the taxa. Five types of trichomes (uni-cellular non-glandular trichome, multi-cellular non-glandular trichome, short-stalked capitate glandular trichome, long-stalked capitate glandular trichome, and peltate glandular trichome) were variable among the taxa as well as their distribution and density. In molecular phylogenetic studies, the genus Glechoma was composed of three geographically distinct major monophyletic groups (Europe-U.S.A., China-Korea, Japan). G. longituba in Korea and China formed well-supported monophyletic group. G. hederacea in Europe and U.S.A. formed a monophyletic and well-supported clade with G. sardoa, which are endemic species in Italy, with G. hirsuta falling as a sister to this clade. However, G. grandis did not form any phylogenetic relationships with the remaining taxa. The ITS analyses provided taxonomic boundaries of taxa in Glechoma although the petal and sepal micromorphological characters provided weak evidences of the systematic value. As further studies, incorporating more DNA regions to the matrix including other additional morphological analysis will be significant to provide clearer taxonomic structure in Glechoma.