• Korean journal of applied entomology
• /
• v.29 no.2
• /
• pp.132-135
• /
• 1990

Three mitochondrial cDNA probes from Aedes albopictus were used to demonstrate restriction site polymorphism in mtDNA of three sibling species of Anopheles quadrimaculatus(Say). It was shown by DNA hybridization to have substantial sequence homology betwen the mtDNA of different genus. The proves reveled local restriction site variation between members of the Anopheles quadrimaculatus sibling species complex. Mitochondrical DNA (mtDNA), isolated from individual mosquitoes was digested by type II restriction enzymes and four enzymes were found to be useful for the purpose. Hind III alone could be used to obtain a diagnostic restriction pattern.

• Animal Systematics, Evolution and Diversity
• /
• v.21 no.2
• /
• pp.137-156
• /
• 2005

A taxonomic study on the Eucyclops serrulatus species group has been accomplished as one of the serial researches on the freshwater cyclopoid copepods in Korea. As a result, the 'Eucyclops serrulatus', hitherto known from Korea through many reports and papers, turns out to be a species complex of six sibling species: E. serrulatus (Fischer), E. roseus Ishida, E speratus (Lilljeborg), E. pacificus Ishida, E. ohtakai Ishida, and I tsushimensis Ishida Taxonomic accounts on their detailed interspecific discrepancies and intraspecific variabilities are presented. A key to the Eucyclops serrulatus group from the Far East is also provided.

• Journal of Korean Library and Information Science Society
• /
• v.49 no.3
• /
• pp.219-240
• /
• 2018

The study analyzed the characteristics of two library and information science journals indexed by Web of Science (WoS) and Scopus in 2015 along with two of their non-indexed sister journals. The study also analyzed the characteristics of a SCIE- and Scopus-indexed oceanographic journal published domestically along with its sister journal indexed by Scopus only. In addition, the study collected and analyzed the articles published during 2014 and 2015 in these 6 journals as well as the citations they received in 2016. By comparing the characteristics and publication data of indexed journals and their sister journals, the study aimed to identify the properties of WoS-indexed journals that may be helpful in enhancing international journal publication. The findings from the analysis of data can be summarized as follows: WoS-indexed journals publish more frequently, focus mainly on articles, are authored by researchers from many countries, and have higher quality papers that receive more citations than their non-WoS-indexed sister journals. The study also found higher rates of co-authored papers in WoS indexed journals, which were amplified in LIS journals. This is an important factor to consider for Korean LIS journals that are dominated by singe-author articles when they prepare to be indexed in WoS.

• Korean Journal of Plant Taxonomy
• /
• v.44 no.1
• /
• pp.39-50
• /
• 2014

Phylogenetic positions of Sedirea and Neofinetia were addressed using the chloroplast matK and the nuclear ITS sequences. We also evaluate the usefulness of the makers for the identification of species and localities. Sedirea and Neofinetia form an independent monophyletic genus, respectively, in both matK and nuclear ITS trees. The sister genus of the Neofinetia was Vanda in both trees. In addition, our trees support the separate recognition of the Neofinetia from Vanda rather than the inclusion of Neofinetia into Vanda. The sister group of the Sedirea was (Dimorphorchis(Pteroceras(Saccolabiun+Phalaeonopsis))) clade. The Dimorphorchis was one of the most probable sister genus to the Sedirea. The sister group relationship between Sedirea and Aerides was suggested by their similar morphology, but not supported in molecular trees. The identification of species and localities of Neofinetia was possible using our two molecular markers. However, several pseudo-gene sequences are discovered from the public data base. In addition, the horizontal gene transfer of chloroplast genomes is frequent events in orchid hybrids. Therefore, we need a careful evaluation for the data prior to systematic use. Generation of sequence data from multiple accessions of a species may helpful to reduce these types of error.

• The Korean Journal of Zoology
• /
• v.31 no.1
• /
• pp.1-10
• /
• 1988

A syrnpatnc sample of Rana nigrnmaculata sand R plancyi (Anura) from Kwangu, Korea was investigated to clarify their taxonornic status and to estimate the degree of genic variation, genetic relatedness, and reproductive isolation between them, based on electrophoretic analysis, marphometric data, mating call, mating season, and habitat. All the characters measured including morphometric, genetic, behavioral, and ecological suggest that they are distinct species and R nigrnmaculata might have originated from R plancyi (tropical form about three and a half million years ago. An adult female was found to be Fl hybrid between two species The hybrid female possessed fettile eggs and morphologically intermediate to the parental species in head parts, hind limb, and web characters. The differences in mating call, mating behavior, breeding season, and habitat strongly suggest that postmating isolating mechanism is operating, even though very rare hybrid is found.

• Korean Journal of Plant Taxonomy
• /
• v.46 no.3
• /
• pp.295-300
• /
• 2016

Sisyrinchium micranthum Cav., a newly naturalized species, was found in Seogwipo, Jeju-do. This species is native to Central and South America and is reported as a widely invasive species around the world. Sisyrinchium micranthum is a sister species of S. rosulatum E. P. Bicknell (Deung-Sim-But-Kkot in Korean), reported as an invasive species earlier in Korea, and both species are members of the subgenus Echthronema. Sisyrinchium micranthum is distinguished from S. rosulatum by the urceolate shape of its flowers and by the large size of its fruits and seeds. Its local name is "Yeon-Deung-Sim-But-Kkot" based on the light blue corolla. We also found that Sisyrinchium angustifolium Mill., previously used for Deung-Sim-But-Kkot, was misapplied. It belongs to the subgenus Sisyrinchium and is morphologically very different from S. rosulatum. We corrected the scientific name of Deung-Sim-But-Kkot by changing it from S. angustifolium to S. rosulatum based on the type specimens, original descriptions, and recent studies.

• Animal Systematics, Evolution and Diversity
• /
• v.16 no.2
• /
• pp.203-211
• /
• 2000

Partial mitochondrial 16S rDNA and COI gene were amplified using PCR and sequenced for four species of oysters in Korea. Phylogenetic relationships among them were inferred from their aligned sequences by neighbor-joining method. The sequence comparison data of two mitochondrial genes showed that the genetic distinction between two oyster genera (Crassostreo and Ostrea) was obvious. Phylogenetic analysis based on the nucleotide sequences and A+T percentage of two genes indicates that C. gigas and C. nippona strongly formed a sister group and then C. ariakensis was clustered with the clade although that based on amino acid sequences of COI gene by neighbor-joining method represented different phylogenetic tree.

• Journal of Life Science
• /
• v.21 no.6
• /
• pp.788-795
• /
• 2011

Accurate identification for pathogenic bacterium is an essential element in the clinical microbiology laboratory. We studied molecular analysis involving the identification of Chyseobacterium indologenes and evaluated the seventeen isolates in Korea with the 16S rRNA gene of the ribosome to estimate phylogenetic relationships within the genus Chyseobacterium in GenBank. The aligned data sets for C. indologenes were 1,176 nucleotides. Sequence variation within the C. indologenes was mostly due to nucleotide substitutions. Korean C. indologenes isolates were not strikingly different from the same species found in the other countries. However, the rates of base substitution in Korean C. indologenes isolates were higher than those of other C. indologenes isolates in GenBank. C. indologenes was placed as a sister species to C. isbiliense, C. hominis, C. hispanicum, C. molle, C. hungaricum, and C. pallidum.

• Journal of Environmental Health Sciences
• /
• v.22 no.1
• /
• pp.91-98
• /
• 1996

산업장이나 생활환경에서 접하기 쉬운 수용성 염화물을 중심으로 19개 원소 24종의 금속화합물이 Chinese Hamster Don 세포에 있어서의 sister chromatid exchange(SCE) 출현빈도에 미치는 영향을 조사하였다. Chinese Hamster Don 세포에 대한 자매염색분체 교환출현빈도의 증가가 $CrO_3, K_2CrO_4, K_2Cr_2O_7, MnCl_2, K_2SeO_3, CH_3HgCl$ (p<0.01), $CoCl_2, Na_2HAsO_4, HgCl_2$ (p<0.05) 9종의 금속화합물에서 나타났으며, dose-response relationships이 현저한 금속화합물은 6가 크로화합물과 $K_2SeO_3$이었다.

• Korean Journal of Ecology and Environment
• /
• v.54 no.3
• /
• pp.156-169
• /
• 2021

Traditional morphological identification difficulties, such as phenotypic plasticity, misidentification of cryptic species, and larval stage species, can be compensated for by using DNA analysis techniques, such as DNA barcoding, in surveying zooplankton populations, including species identification. Recently, the rapid development of DNA sequencing techniques has allowed DNA-based community analysis not only for zooplankton assemblages in various aquatic ecosystems but also for the gut contents of zooplankton that are limited by conventional methods such as visual and microscopic identification. Therefore, the application of DNA sequencing can help understand biological interactions through the analysis of zooplankton food sources. The present paper introduces the major DNA-based approaches in zooplankton research topics, including taxonomic approaches by DNA barcoding, community-level approaches by metabarcoding, and gut content analyses, summarizes the analysis methods, and finally suggests the methodological topics that need to be considered for future applications.