• Journal of the Korean Ceramic Society
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• v.38 no.1
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• pp.22-27
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• 2001

$\beta$-Si$_3$N$_4$종자입자 첨가가 소결조제로 Y-Mg-Si-Al-O-N계 oxynitride glass를 사용하여 일축가압 소결을 행한 SiC-Si$_3$N$_4$복합재료의 미세구조와 기계적 특성에 미치는 영향을 고찰하였다. 길게 자란 $\beta$-Si$_3$N$_4$입자들과 등방성의 $\beta$-SiC 입자들이 균일하게 분포된 미세구조를 얻었다. $\beta$-Si$_3$N$_4$종자입자 함량이 증가함에 따라 SiC-Si$_3$N$_4$복합재료의 강도와 파괴인성이 증가하였고, 이는 복합화에 기인하는 결함크기의 감소와 길게 자란 $\beta$-Si$_3$N$_4$입자에 의한 균열가교 및 균열회절 등에 기인하였다. SiC-70 wt% Si$_3$N$_4$복합재료의 대표적인 강도와 파괴인성은 각각 770 MPa과 6.2 MPa.m$^{1}$2/ 이었다.

• Journal of The Korean Society of Grassland and Forage Science
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• v.8 no.3
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• pp.117-122
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• 1988

다른 生長條件(growth chamber, outdoor)과 室素施肥條件(N 0, N 60kg $ha^{-1}$')에서 자란 Perennial rye- grass잎에 있어서 葉伸長率(LER)과 伸底部位의 空間的 移動 및 細胞의 伸長등을 알아보고자, 잎 基部내 伸長部位의 O~30mm에 pin처리를 하고 일정시간후 Pin hole의 이동거리로서 長을 조사하었으며 표면복 제방법에 의해 細胞의 길이를 측정하였다. Growth chamber에서 자란 목초의 LER은 25.2mm $day^{-1}$(outdoor 목초에 비해 54.6% 증가), 생육의 공 간분포는 基部로부터 24mm까지, 최대생육은 10~13nm에서, 세포신장은 20nm까지로 관찰되었다. Outdoor 목초는 LER 16.3nm, 생육분포는 17mm까지, 최대생육은 5 ~8mm에서, 세포신장은 14mm까지었다. 질소시비구 목초의 LER은 30.3nm$day^{-1}$(무시비구 목초에 비해 61, 2% 증가), 생육분포는 基部로부터 27 mm까지, 최대생육은 13~1Smm에서, 세포신장은 21mm까지었으며, 질소무시비구 목초는 LER 21.3mm, 생육 분딘는 21mm까지 최대생육은 8~llmm에서, 세포신장은 16mm까지였다. Ligule 부위의 세포길이는 현저히 짧아졌으며, LER이 빠를때 伸長部位내 生育의 移動速度도 빨랐다.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.501-504
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• 2001

The effect of soil EC on tissue morphology of leaf and shoot tip in cucumber (Cucumis sativus L. cv. Euinchim-baekdadagi) seedlings was investigated. Number of trichomes on leaf upper epidermis increased with the increase in soil EC from 1.0 to $3.0dS{\cdot}m^{-1}$, but the shape and number of stomata on lower epidermis remained unchanged. Epidermal cells of cucumbers grown in EC $1.5dS{\cdot}m^{-1}$ soil was occupied mostly by large vacuole whereas those grown in EC $3.0dS{\cdot}m^{-1}$ soil were filled with a nucleus, mitochondria, chloroplast and other micro-organelles. Sponge parenchima cells were also larger and contained fewer chloroplasts at EC $1.5dS{\cdot}m^{-1}$ than those grown at EC $3.0dS{\cdot}m^{-1}$. Leaf thickness decreased at high EC and the color of epidermal cells became significantly darker on the photograph of optical microscope. Normal tissue differentiation was greatly suppressed in plants grown in soils with $3.5dS{\cdot}m^{-1}$ or higher EC.

• Korean Journal of Animal Reproduction
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• v.22 no.4
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• pp.419-424
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• 1998

The present study was carried out to examine the efficiency of cloning of transgenic embryos by nuclear transfer(NT) using gene-injected rabbit embryos. The rabbit embryos at pronuclear stage were microinjected with methallothionein-human growth hormone(MT-hGH) gene and cultured to 8- and 16-cell in TCM-199 containing 10% FCS with a monolayer of rabbit oviductal epithelial cells in a 5% $CO_2$incubator. The recipient oocytes were collected from the oviducts 14~16 h after hCG injection. The oocytes were enucleated and activated with 5$\mu$M ionomycin and 2mM 6-dimethylaminopurine. Blastomeres form gene-injected embryos were transferred into the enucleated oocytes by micromanipulation. The nuclear transplant oocytes were electrofused and co-cultured with rabbit oviductal cells. Following 120 h of culture, blastocysts were prepared for gene analysis by polymerase chain reaction(PCR). In previous experiment, the rate of gene-positive embryos detected by the nested PCR analysis was significantly decreased while developing to blastocyst(25%)(Kang et al., 1998). The fusion rate of gene-injected blastomeres was significantly(P＜0.05) lower than non-injected blastomeres(66% vs 80%). However, the NT embryos that were derived from gene-injected donor embryos did not differ from control embryos in development to the blastocyst stage(39% vs 31%). Of the 43 NT blastocysts developed from the gene-injected donor embryos, twelve(28%) were positive for the injected DNA. The results indicate that NT with gene-injected embryos can be successfully used for cloning and multiplication of transgenic embryos, furthermore applicable to improvement of transgenic animal production.

• Current Research on Agriculture and Life Sciences
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• v.28
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• pp.47-52
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• 2010

This study was carried out to investigate the availability of covering material used for raising seedlings in the rice nursery bed. The pre-germinated seeds of a rice cultivar 'Chilbo' were sown into seedling tray ($60{\times}30{\times}2.5cm$) on 20th April and 3rd May 2010. Polypropylene spunbonded fabric (PSF) and lagging were used for covering materials in rice nursery bed. The growth characteristics of seedlings were evaluated at 8, 15 and 25 days after sowing (DAS) in the nursery beds covered with PSF and lagging, respectively. The plant height of 8-day old seedlings grown in lagging nursery bed was longer by 2cm than those in PSF nursery bed when the seeds were sown on 20th April, but seedling quality grown in lagging nursery bed was poor compared with that in PSF nursery bed. The growth characteristics of 15-day old seedlings were similar to that of 8-day old seedlings. The plant height of 8, 15 and 25-day old seedlings grown in lagging nursery was longer than those in PSF nursery when the seeds were sown on 3rd May. The seedling quality grown in PSF nursery was better than that in lagging nursery. The seedlings grown in PSF nursery have more leaves and better seedling quality compared with that of lagging nursery. The seedling quality was remarkably decreased with the increase of nursery period in lagging bed but seedlings quality were recovered at 5 days after hardening treatment by removing the lagging.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.40 no.1
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• pp.29-36
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• 2014

Dendrobium candidum is one of the well-known orchid on traditional and rare medicinal herb in China. We artificially cultured protocorm-like body and multiple shoots using the bioreactor culture system from Dendrobium candidum and experimented an efficacy as a cosmetic ingredient for skin care. Several studies previously have reported anti-tumor, wound healing and immunological function of Dendrobium candidum but other efficacies were not well known. In the present study, we investigated the cosmetic efficacy to know applicable value of protocorm-like body and multiple shoots cultured from Dendrobium candidum as a cosmetic ingredient. The biological activities of extracts from protocorm-like body, multiple shoots, aerial part and underground part of Dendrobium candidum were investigated. Results were found that extracts of protocorm-like body are superior to other extracts (underground part, aerial part and multiple shoots extracts) on anti-oxidant effect. Also, protocorm-like body extract contained the phenolic and flavonoid compounds more than aerial part, underground part and multiple shoots extracts. In addition, we investigated skin whitening effect related to whitening of skin. In tyrosinase activity and melanin synthesis assay, multiple shoots extract is superior to other extracts (aerial part, underground part and protocorm-like body), on inhibitory effect of tyrosinase activity and melanin synthesis respectively. These results indicate that the protocorm-like body and multiple shoots extracts cultured from Dendrobium candidum presents a new possibility of being applicable to skin improvement as a cosmetic ingredient.

• Journal of Plant Biotechnology
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• v.39 no.4
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• pp.281-287
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• 2012

This experiment was conducted to investigate the effect of various type of Magma seawater (MSW) concentrations on plant growth and useful mineral contents in Bletilla striata. In the RO (Reverse Osmosis) and ED (Electronic Distal) treatment, hardness of medium was poored in 3.0 g/L gelrite but increased in 8.0 g/L plant agar, 38,000 and $2,000g/cm^2$ respectably. We analyzed the morphological and physiological characteristics differences of B. striata treated various MSW. Survival frequency of plant and growth (shoot length, shoot diameter, root length, root diameter, shoot/root ratio) were significantly increased in RO and ED treatment at 50% and 10%, especially. Chlorophyll contents in ED treatments were higher than those in control and RO treatment. The content of strontium (Sr) in 20, 50, 75, 100% ED treatment, were higher than those in the control and RO, ED 1, 5, 10% treatment. These results showed that treatment of ED with the range of 20~100% could be used to supply the strontium enriched orchid plant. It is considered that MSW may be applied for use in Magma seawater to promotion of growth and produced functional plant.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.5
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• pp.619-628
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• 1995

Crude aqueous extracts from dried leaves, stems, roots, and flowers from both field grown and greenhouse grown alfalfa plants inhibited alfalfa seed germination and seedling growth. The degree of inhibition was greater in the field grown plant extracts. Flowers extract of field grown plant most inhibited alfalfa germination and seedling growth. In the concentration study, the highest concentration of extract (9.0%, w/v) significantly inhibited total alfalfa seed germination by 50% as compared to control. In partitioning study using pot hydroponic culture of plant biomass into leaves, stems, root, LAR products of LWR and SLA exhibited significant variation among four species. This result support that the inhibitory effect of autotoxic substances presenting in alfalfa tissue may be possible interference with the patitioning of biomass into leaf component relative to the total biomass produced by the alfalfa plant. Toxicity of extract was not reduced by adding activated charcoal, Dowex-50W, amberlite to the extract. Toxic substances existing in most plant tissues but mainly above ground foliage are water soluble and stable and may persist in old alfalfa fields. Thus, it is recommended to remove as much as possible of the above growth parts, especially vegetative stage, before one tries to re-establish alfalfa in former field of alfalfa.

• Ecology and Resilient Infrastructure
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• v.6 no.4
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• pp.295-303
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• 2019

This study investigated the effects of various cultivation pots on Betula platyphylla var. japonica seedlings in order to select suitable pots for the production of healthy seedlings for the ecological revegetation. We used six types of the pots natural pot, natural pot with inside 5 mm width root turning bump, natural pot with inside 10 mm width root turning bump, negative-phototropism pot, negative-phototropism pot with inside 5 mm width root turning bump, and negative-phototropism pot with inside 10 mm width root turning bump. As greenhouse cultivation provided a high level of control of irrigation and temperature, the seedlings did not show any significant differences in plant height, number of branches, and diameter at root collar, but showed a significant difference in root growth among the different types of pots. The root growth was best in the negative-phototropism pot. In the negative-phototropism, the roots grew in a vertical shape, while they grew abnormally in a spiral shape in the natural pots. In outdoor cultivation, the growth of seedlings showed significant differences according to container types. The seedlings grown in specially manufactured negative-phototropism pots were most excellent in all of the height, number of branches, diameter at root collar and root growth. As for the seedlings grown in the negative-phototropism pots, their roots grew vertically and thus their rooting was well established after they were transplanted outdoor. A size of less than 5 mm ridge installed in the negative-phototropism was found to be appropriate for the seedling growth. These results showed that the negative-phototropism pot would be best suitable for the growth of Betula platyphylla var. japonica seedlings and for the production of seedling for ecological revegetation.

• Journal of Bio-Environment Control
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• v.25 no.3
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• pp.139-145
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• 2016

The objective of this study was to compare the growth and light use efficiency of red leaf lettuce grown under three types of combined light-emitting diodes (LEDs) and fluorescent lamps (FL) in a closed-type plant production system. The eighteen days-old lettuce seedlings of red leaf lettuce (Lactuca sativa L., 'Jeokchima') were transplanted to the close-type plant production system equipped with three types of combined LEDs with red (R, 655 nm), blue (B, 456 nm), green (G, 515 nm), and white (W, 456 nm + 558 nm) (R:B=8:2, R:W:B=8:1:1, R:G:B=8:1:1) and FL. The seedlings were grown under normal growth conditions ($20^{\circ}C$, $181{\pm}4{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 12 h photoperiod) for four weeks. Lettuce plants grown under FL had significantly higher leaf shape index than those under all LED treatments. Although growth of shoots and roots was not show any significant difference among LED treatments, all of the LED treatments induced about 34% higher shoot fresh weight than that of the FL. On the other hands, the total power consumption of FL was 145 kW for 4 weeks, while the mean value of LED treatments was 54 kW, which was about 3 times lower value than that of the FL. The light use efficiency based on dry matter in LED treatments was about 34 mg/W and this was about 3.5 times higher energy saving value than the FL. In conclusion, this study showed that irradiation of optimal combined LEDs in closed-type plant production systems can improve the lettuce growth as well as maximize in light use efficiency through energy saving than the FL.