Browse > Article
http://dx.doi.org/10.5010/JPB.2012.39.4.281

In vitro culture of rare plant Bletilla striata using Jeju magma seawater  

Bae, Kee-Hwa (Hongcheon Institute of Medicinal Herb)
Kim, Ki Ju (Jeju MSW Research Institute, Jeju Technopark)
Kim, Nam Young (Department of Horticulture and Education, Korea National Arboretum)
Song, Jae Mo (Nature Environment Research Office of Gangwon Province)
Publication Information
Journal of Plant Biotechnology / v.39, no.4, 2012 , pp. 281-287 More about this Journal
Abstract
This experiment was conducted to investigate the effect of various type of Magma seawater (MSW) concentrations on plant growth and useful mineral contents in Bletilla striata. In the RO (Reverse Osmosis) and ED (Electronic Distal) treatment, hardness of medium was poored in 3.0 g/L gelrite but increased in 8.0 g/L plant agar, 38,000 and $2,000g/cm^2$ respectably. We analyzed the morphological and physiological characteristics differences of B. striata treated various MSW. Survival frequency of plant and growth (shoot length, shoot diameter, root length, root diameter, shoot/root ratio) were significantly increased in RO and ED treatment at 50% and 10%, especially. Chlorophyll contents in ED treatments were higher than those in control and RO treatment. The content of strontium (Sr) in 20, 50, 75, 100% ED treatment, were higher than those in the control and RO, ED 1, 5, 10% treatment. These results showed that treatment of ED with the range of 20~100% could be used to supply the strontium enriched orchid plant. It is considered that MSW may be applied for use in Magma seawater to promotion of growth and produced functional plant.
Keywords
Bletilla striata; in vitro; propagation; magma seawater;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Korea Forest Service (2009) Rare plants data book in Korea. Korea National Arboretum Pocheon pp 296
2 Kyung, YJ, Yoon MJ, Park CH (2000) Identification of the genetic polymorphism of Bletilla striata using RAPD. Kor J Hort Sci Technol 18(2):103-106
3 Lee, CK, Cho KC, Lee JH, Cho JY, Seo BS, Yang WM (2005) Effects of selenium supplying methods on the growth and Se uptake of hydroponically grown Tomato plants. J Bio-Environ Con 14(4):284-288   과학기술학회마을
4 Lee, TB (1993) Illustrated flora of Korea. Hyangmunsa Seoul pp 990
5 Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Meth Enzymol 148:350-382   DOI
6 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-479   DOI
7 Nelson, PV (2003) Greenhouse operation and management. 6th ed. Prentice Hall
8 Paek, KY, Jeon ES (1995) Stomatal density, size and morphological characteristics in Orchids. J Kor Soc Hort Sci 36(6): 851-862
9 Robertson, GL (1985) Changes in the chlorophyll and pheophytin concentration of kiwifruit during processing and storage. Food Chem 17:25-31   DOI
10 Yoo, YK, Kim HK, Choi KH (2001) Regulation of growth and flowering by low temperature treatment in Bletilla striata. J Kor Soc Hort Sci 42(2):227-232
11 Yoo, YK, Oh CG, Park CH (2000) Geographical distribution and vegetation of Bletilla striata Habitats. J Kor Soc Hort Sci 41(2):212-216
12 Yun, JS, Hong EY, Kim IH, Shin SK, Yun T, Lee CH, Kim TS, Paek KY (2004) Breeding of a new tetraploid Bletilla striata Reichb. fil., 'Chungbuk Jaran'. Kor J Hort Sci Technol 22(4):495-498   과학기술학회마을
13 Winsor, G, Adams P (1987) Diagnosis of mineral disorder in plants. Glasshouse crops. Her Majesty's Stationery Office, London Vol. 3.
14 Buckl,e KA, Edwards RA (1970) Chlorophyll degradation and lipid oxidation in frozen unblanched peas. J Sci Food Agric 21:307-314   DOI
15 Cheong, YH, Han MJ, Sung SJ, Seo DC, Kang JG, Sohn BK, Heo JS, Cho JS (2009) Effects of selenium supplement on germination, sprout growth and selenium uptake in four vegetables. Kor J Environ Agric 28:179-185   과학기술학회마을   DOI
16 Chretien, S (2000) High electrical conductivity and radiation-based water management improve fruit quality of greenhouse tomatoes grown in rockwool. Hot Sci 35:627-631
17 Chung, JD, Suh JH (1982) Studies on asymbiotic germination of seeds of Bletilla striata. Kor J Plant Tissue Cult 9(1):27-33
18 Combs, GF, Combs SB (1986) The Role of Selenium in Nutrition Academic Press Inc New York NY
19 Jung, SJ, Kim GE, Kim SH (2001) The changes of ascorbic acid and chlorophylls content in Gochu-jangachi during fermentation. Korean J Soc Food Sci 30:814-818   과학기술학회마을
20 Kim, AD, Kang KA, Zhang RC, Lim M, Jee YE, Lee NH, You HJ, Ko KS and Hyun JW (2010a) Reactive oxygen species scavenging effects of Jeju waters containing vanadium components. Cancer Prev Res 15:111-117
21 Kim, AD, Kang KA, Zhang R, Piao MJ, Kim SM, Jee YE, Lee NH, You HJ, Ko KS and Hyun JW (2010b) Effects of Jeju water containing vanadium on antioxidant enzymes in vitro. Cancer Prev Res 15:262-267
22 Kim, AD, Kang KA, Zhang R, Piao MJ, Kim SM, Jee YE, Lee NH, You HJ, Ko KS and Hyun JW (2011) Antioxidant enzyme-enhancing effects of Jeju water containing vanadium in vivo. Cancer Prev Res 16:58-64