• Clinical and Experimental Pediatrics
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• v.49 no.5
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• pp.558-564
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• 2006

Purpose : Transcranial electromagnetic stimulation(TMS) is a noninvasive method which stimulates the central nervous system through pulsed magnetic fields without direct effect on the neurons. Although the neurobiologic mechanisms of magnetic stimulation are unknown, the effects on the brain are variable according to the diverse stimulation protocols. This study aims to observe the effect of the magnetic stimulation with two different stimulation methods on the cultured hippocampal slices. Methods : We obtained brains from 8-days-old Spague-Dawley rats and dissected the hippocampal tissue under the microscope. Then we chopped the tissue into 450 µm thickness slices and cultured the hippocampal tissue by Stoppini's method. We divided the inserts, which contained five healthy cultured hippocampal slices respectively, into magnetic stimulation groups and a control group. To compare the different effects according to the frequency of magnetic stimulation, stimulation was done every three days from five days in vitro at 0.67 Hz in the low stimulation group and at 50 Hz in the high stimulation group. After N-methyl-D-aspartate exposure to the hippocampal slices at 14 days in vitro, magnetic stimulation was done every three days in one and was not done in another group. To evaluate the neuronal activity after magnetic stimulation, the $NeuN/{\beta}$-actin ratio was calculated after western blotting in each group. Results : The expression of NeuN in the magnetic stimulation group was stronger than that of the control group, especially in the high frequency stimulation group. After N-methyl-D-aspartate exposure to hippocampal slices, the expression of NeuN in the magnetic stimulation group was similar to that of the control group, whereas the expression in the magnetic non-stimulation group was lower than that of the control group. Conclusion : We suggest that magnetic stimulation increases the neuronal activity in cultured hippocamal slices, in proportion to the stimulating frequency, and has a neuroprotective effect on neuronal damage.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.35 no.12B
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• pp.1192-1198
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• 2010

As indicators of life quality have recently shown great improvement, alternative medicine is becoming a mainstream health care pattern, in which early stage medical examinations and treatments are usually preformed before diseases occur. In this context, the ear-acupuncture method is increasingly used these day as one of important alternative medicine treatments. In this paper, we performed an experiment of facial region color analysis with kidney ear acupuncture point irritation. For this, we collected facial image samples before and after kidney ear acupuncture point irritation under the same condition. From the samples, the change rates of K, L values of CMYK color system and Lab color system, respectively, on kidney related facial parts were extracted and analyzed to investigate kidney function improvement.

• Journal of Korean Physical Therapy Science
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• v.16 no.3
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• pp.1-9
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• 2009

Background: The purpose of this study is to have examined the influence on the blood circulation by comparing the differences between stimulating the sympathetic ganglion and the muscle group among the stimulation variables in interferential current stimulation. Method: The object of the study is the twenties(M=8, F=12), who are in great condition and have no pathological report for the blood circulation influence. The intensity of the inferential current stimulation is the medium degree, 100 bps constant current, which is the comfort and degree to confirm the muscle contraction. The areas stimulated are the stellate ganglion area in the seventh cervical vertebrae and the forearm muscle area. Results: We have made sure that there is no change in blood pressure and pulse and that the change in the skin temperature occurred highly. Conclusion: In considering the change of the blood circulation in case of stimulation area by the inferential current stimulation, we have seen that stimulating the sympathetic ganglion area is more effective than stimulating muscle area directly.

• Proceedings of the KSMRM Conference
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• 2001.11a
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• pp.102-102
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• 2001

목적： 이중언어자에 있어서 각각의 언어가 대뇌에 어떻게 표상 되어 있는가에 대한 연구가 활발히 진행되고 있다. 본 연구에서는 한국어/중국어 이중언어화자를 대상으로 사용한 언어와 과제에 따라 활성화된 부위에 차이가 있는지 알아보고자 하였다. 대상 및 방법： 이중언어자(화교) 자원자 8명을 대상으로 하였고, 모두 오른손잡이였다. 한국어와 중국어로 구성된 어휘판단과제(lexical decision task)와 의미판단과제 (semantic decision task)를 이용하여 언어 영역의 활성화를 유도하였다. 어휘판단과제는 화면에 제시되는 두 자극이 모두 단어인지 아닌지 판단하여 신호하도록 하였고(예：원개 · 교화, 토끼, 지욱), 의미판단과제는 화면에 제시되는 두 자극의 의미가 관련 있는지 없는지 판단하여 신호하도록 하였다. (예：가가 ·제제, 아침·저녁). 대조과제는 아랍어(예：equation omitted) 글자의 크기를 판단하여 신호하도록 하였다. 1.5T 초전도 자기공명영상장치에서 EPI BOLD 기법을 이용하여 기능적 영상을 얻었으며 8명에서 얻은 영상을 정상화(normalize)한 후 SPM 프로그램을 이용하여 통계분석을 하였다. p<0.000001을 기준으로 얻어진 활성화 영상에서 각 언어와 과제별로 나타난 활성화 신호의 차이를 육안적으로 분석하였다.

• Proceedings of the KSMRM Conference
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• 2001.11a
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• pp.106-106
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• 2001

목적： 일반인과 외상성 스트레스 장애환자들(PTSD)에서 동일한 공포 자극에 대한 기능적 뇌 자기ㆍ공명영상에서 뇌 활성화 부위의 차이를 알아보고자 하였다. 대상 및 방법： 대조군 (여자9명)과 외상성 스트레스 장애로(PTSD) 진단된 환자군 (여자9명)을 대상으로 하였고 모두 오른손잡이였다. 외상성 스트레스 장애군은 모두 교통사고와 관련되어 있었다. 1.5T MR 기기에서 EPI BOLD 기법을 이용하여 24개의 axial slice를 얻었으며, 시각자극으로 교통사고 현장 사진과 비교군으로 체크 무의 사진을 무작위로 배열하여 각각 1초씩 보여 주었다 (event related design). SOA (Stimulus Onset Asynchrony)는 3.5초로 하였고 전체 영상은 10분 동안 얻었다. 모든 환자에서 기능적 자기공명 영상은 1차례 시행하였다. 영상후 처리는 SPM 분석 프로그램을 사용하였으며 황성화 신호의 유의수준은 p=0.01을 기준으로 활성화 영상을 얻었다. 활성화 신호를 육안으로 비교 분석하였고 해마, 편도핵, 전전두엽의 활성화 정도를 중심으로 평가하였다.

• Journal of Chest Surgery
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• v.32 no.9
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• pp.844-846
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• 1999

Chronic irritation to arterial wall by foreign material may give rise to delayed vascular injury. A 50 years old male patient with kyphoscoliosis had undergone fixation of orthopedic Cotrel-Dubousset(CD) rods and screws. Fourteen months after that surgery, a false aneurysm of the descending thoracic aorta associated with pulsating hematoma in the muscular chest wall developed. The false aneurysm was managed by resecting the diseased aortic segment and replacing the vascular graft.

• Korean Journal of Ichthyology
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• v.19 no.4
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• pp.266-273
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• 2007

Heat shock proteins (HSPs) are a family of highly conserved proteins playing an important role in the functioning of unstressed and stressed cells. The HSP70 family, the most widely studied of the hsps, is constitutively expressed (hsc70) in unstressed cells and is also induced in response to stressors (hsp70), especially those affecting the protein machinery. The HSP/HSC70 proteins act as molecular chaperones and are crucial for protein functioning, including folding, intracellular localization, regulation, secretion, and protein degradation. Here, we report the identification and characterization of the putative amino acid sequence deduced from one cDNA clone identified as heat shock protein 70. The alignment showed that the putative sequence is 100% identical to the heat shock protein 70 cognate (HSC 70) of olive flounder. The 5'-flanking region sequence (approximately 1 kb) ahead of the hsc70 gene was cloned by genome walking and a putative core promoter region and transcription elements were identified. We characterized the promoter of the olive flounder hsc70 gene by examining the ability of 5'-upstream fragments to drive expression of green fluorescent protein (GFP) in live embryos.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.15 no.3
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• pp.645-653
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• 2011

The aim of this study was to develop a Pulsed Magnetic Fields(PMFs) system which can produce effects locally and simulate muscular tissues equally. To evaluate the PMFs system we caused DOMS(Delayed Onset Muscle Soreness) to subjects in biceps of the arm. Then, we stimulated acupoint HT2 using PMFs(20 minutes) and TEAS(20 minutes) for 2 days. The other subjects did not stimulate. Then we checked body surface temperature in biceps of the arm. All subjects had an asymmetrical body surface temperature in biceps after exercise(Non-stimulation group=$2.00{\pm}1.16^{\circ}C$, TEAS group=$1.73{\pm}0.52^{\circ}C$, PMFs group=$1.48{\pm}0.51^{\circ}C$). After 1st stimulation all subjects had decreased temperature differences(Non-stimulation group=$1.37{\pm}0.71^{\circ}C$, TEAS group=$1.08{\pm}0.43^{\circ}C$, PMFs group=$1.23{\pm}0.15^{\circ}C$). PMFs group had a symmetry body surface temperature after 24 hours($0.05{\pm}0.06^{\circ}C$) and TEAS group had that after 48 hours($0.1{\pm}0.08^{\circ}C$). Non-stimulation group did not recovery after 48 hours($0.37{\pm}0.06^{\circ}C$). Therefore, PMFs on acupoint had an therapeutic effect in DOMS.

• The Korean Journal of Physiology
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• v.24 no.1
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• pp.181-194
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• 1990

Antidromically activated spinoreticular tract (SRT) cell units in the lumbosacral enlargement of ${\alpha}-chloralose$ anesthetized cats were classified as medial and lateral SRT units according to the location of their axonal termination. Identified SRT units were tested fer antidromic conduction velocity, laterality of their axonal projection, the location in spinal gray, peripheral receptive field, the response pattern to graded mechanichal stimulation and the responsiveness to $A{\delta}$ and C volley of the peripheral nerve. 1) The 59% of 34 medial SRT units were recorded in ipsilateral side to the antidromic stimulation site, but 60% of the 47 lateral SRT units projected to contralateral side. 2) Most of the medial SRT cells and rostral ventrolateral medulla (RVLM)-projecting lateral SRT cells were recorded in lamina VII & VIII. The LRN (lateral reticular nucleus)-projecting SRT cells, however, distributed through all the laminae except superficial ones (I & II). 3) The identified SRT units were classified as low theshold (LT), deep, high threshold (HT), wide dynamic range (WDR) cells, based on the response patterns to graded mechanical stimuli. The proportion of SRT units which receive noxious input was 37.5%, 25% and 75% in the medial, LRN-projecting and RVLM SRT group, respectively. 4) There was no significant difference in the mean conduction velocities between the 3 groups. But the deep cells had significantly higher velocity than that of the HT cells. The above results show that the peripheral inputs to the SRT units are different in the 3 groups: medial, LRN & RVLM SRT group. Especially in case of the SRT cells projecting to RVLM which is a probable candidate fur the integration center of various pressor reflexes such as somatosympathetic reflex, the noxious informations occupy higher proportion of input to them than in other groups. Therefore the noxious information transmitted through the lateral SRT destined for RVLM is expected to play a role in somatosymapthetic reflex.

• Parasites, Hosts and Diseases
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• v.27 no.3
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• pp.217-224
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• 1989

Four females and a male nematode isolated from 2 patients who visited eye clinics In Seoul were identified as Thelazia callipaeda and their ultrastructures were observed by scanning electron microscopy(SEM). General features of the worms were slender and attenuated at both ends. Vaginal opening was located at 0.27 mm from the anterior end, and in front of the esophagointestinal junction. In the body cuticle transverse striations varied characteristically through the body. The number of cuticular transverse striations was 400∼650/mm at head portion, 250/mm at middle portion and 300∼350/mm at tail portion. The SEM observation of the mouth part of the females showed 6 cord-like cuticular thickenings in hexagonal arrangement and an amphid was observed. A lateral line, a vaginal opening, a pair of phasmids, and an anus were identified in the body portion. A pair of papillae and 6 cord-like cuticular thickenings were on the mouth part of the male. It was difficult to observe structures at the tail of the male except wrinkle-like structures. Most of the larvae isolated from the uterus of a female worm were sheathed and thus cuticular striations were not Eren. Others were unsheathed and revealed cuticular striations. The oval membrane which encysted sheathed larvae was also observed. These are the 18th and 19th record of human thelaziasis in Korea as the literature are concerned.