• Clinical and Experimental Reproductive Medicine
• /
• 제24권2호
• /
• pp.261-265
• /
• 1997

본 연구는 EGF가 체외수정 및 체외배양에 의해 생산된 생쥐배의 체내 발달에 미치는 영향을 조사하고자 실시하였다. 본 실험에 사용된 난자는 체외수정 후 얻어진 2-세포기배를 EGF 첨가유무에 따라 배양 (5-6 embryos/25${\mu}l$/drop)하여 얻어진 4일령의 배반포기배로서, 각 처리군의 배반포기배는 가임신 3일된 대리모의 자궁내에 이식되어졌다. 그 결과를 요약하면 다음과 같다. 1,2-세포기배를 EGF의 첨가유무에 따라 배양하여 배반포기배로의 발달율과 세포수를 조사하였던 바, 처리군간의 유의한 차이는 나타나지 않았다. 2. 하지만, 각 처리군에서 회수된 배반포기배의 체내 발달을 조사하였던 바, 총 수태율의 결과에 있어서는 대조군과 EGF 처리군 각각 64.4%와 69.8%로서 두 군간에 유의한 차이를 나타내지 않았지만, 정상태아 발생율에 있어서는 EGF 처리군 (51.2%)이 대조군 (31.1%)보다 매우 높게 나타냈다. 따라서, 비록 EGF 처리군이 대조군과 비교하여 볼 때 체외수정 및 체외배양에 의해 생산된 난자의 유의한 발달은 나타내지 않았지만 난자의 질적인 향상을 통해서 체내발달을 증진시킬 수 있을 것으로 사료된다.

• 대한한방부인과학회지
• /
• 제21권4호
• /
• pp.104-127
• /
• 2008

Purpose: This study was performed to investigate the effects of Dangguijakyaksan on the development of experimentally-induced endometriosis in rats. Methods: Endometriosis was induced in rats by autotransplanting uterine tissue to the peritoneum and divided them into three groups: (1) sham-operated group(n=8). (2) surgically induced endometriosis and untreated control group(n=8). (3) surgically induced endometriosis and Dangguijakyaksan treated group. Dangguijakyaksan was orally administrated for 15 days after operation. Then we measured the body weight. the volume of endometriotic implants. the weight of uterus and ovary. and analysed the concentration of cytokines (MCP-1, TNF-$\alpha$, IL-1$\beta$, IL-6) in peritoneal fluids. Histopathology, immunohistochemistry for COX-2 and VEGF. and histochemistry for mast cell in transplanted uterine tissue were also performed. Results: - The volume($mm^3$) of endometriotic implants in Dangguijakyaksan treated group was significantly decreased compared with control group. - The concentration(pg/ml) of MCP-1, TNF-$\alpha$ and IL-l$\alpha$ in peritoneal fluids in Dangguijakyaksan treated group was significantly decreased compared with control group. - Histopathologically, proliferation of endometriotic epithelia. infiltration of inflammatory cells and angiogenesis in transplanted uterine tissue of Dangguijakyaksan treated group were weakly observed than those of control group. - The percentage of positive epithelial layers for COX-2 in Dangguijakyaksan treated group was significantly decreased compared with control group. - The VEGF expression of endometriotic epithelia, neovascular endothelia and stromal cells in transplanted uterine tissue of Dangguijakyaksan treated group were weakly observed than those of control group. - The number of mast cells in transplanted uterine tissue in Dangguijakyaksan treated group was significantly decreased compared with control group. Conclusion: On the basis of these results. we concluded that Dangguijakyaksan has inhibiting effects on the development of transplanted uterine tissue. And these effects may be related with decreased production of MCP-1, TNF-$\alpha$ and IL-1$\beta$, and decreased expression of COX-2 and VEGF, and inhibition of mast cell infiltration by administration of Dangguijakyaksan.

• 대한한방부인과학회지
• /
• 제27권1호
• /
• pp.101-119
• /
• 2014

Objectives: This study was performed to investigate the effects of Hominis Placenta pharmacopuncture (HPP) therapy on the experimentally-induced endometriosis in the rats. Materials and Methods: Endometriosis was induced in rats by autotransplanting uterine tissue to the peritoneum and divided them into three groups: (1) sham-operated group (n=8), (2) surgically induced endometriosis and untreated control group (n=8), (3) surgically induced endometriosis and HPP treated group. Sham-operated group and control group were inject with normal saline once a every other day for 30days, while treated group was injected with HPP extract once a every other day for same duration. Injected point of HPP and normal saline were subcutaneous tissue at Gwanwon (CV4) acupoint. Then we measured the body weight, the volume of endometriotic implants, the weigh of uterus and ovaries, and investigated the concentration of cytokines (MCP-1, TNF-${\alpha}$) in peritoneal fluids. Histopathology, immunohistochemisty for COX-2 and VEGF, and histochemistry for mast cell in transplanted uterine tissue were performed. Results: The volume ($mm^2$) of endometriotic implants in HPP treated group ($55.4{\pm}41.6$) was significantly decreased (p<0.01) compared with control group ($140{\pm}66.1$). And the concentration (pg/ml) of MCP-1 in peritoneal fluids in HPP treated group ($1117.6{\pm}60.5$) was significantly decreased (p<0.01) compared with control group ($1446.2{\pm}280.3$). The concentration (pg/ml) of TNF-${\alpha}$ in peritoneal fluids in HPP treated group ($80.6{\pm}31.4$) was decreased (p<0.01) compared with control group ($145.3{\pm}86.9$). Histopathologically, proliferation of endometriotic epithelia, infiltration of inflammatory cell and angiogenesis in transplanted uterine tissue of HPP treated group were weakly observed than those of control group. The COX-2 expression in endometrial, epithelial and stromal cells in transplanted uterine tissue of HPP treated group was decreased compared with control group. The VEGF expression of endometriotic epithelia, neovascular endothelia and stromal cell in transplanted uterine tissue of HPP treated group were weakly observed than those of control goup. Conclusions: HPP is effect on Endometriosis of rats by Experimentally-induced.

• 한국산학기술학회논문지
• /
• 제22권5호
• /
• pp.465-474
• /
• 2021

본 연구는 결혼이주여성을 대상으로 HPV 지식, HPV 백신 접종 태도 및 HPV 백신 접종 의도를 확인하고, HPV 백신접종 의도에 미치는 영향요인을 파악하기 위한 서술적 조사연구이다. 본 연구는 J도 지역에 거주하는 결혼이주여성 136명을 대상으로 하였다. 연구 도구는 HPV 지식, HPV 백신 접종 태도, HPV 백신 접종 의도를 사용하였으며, 자료수집기간은 2017년 9월 28일부터 12월 21일까지였다. 수집된 자료는 SPSS/WIN 24.0 프로그램을 이용하여 분석하였다. 본 연구결과, 결혼이주여성의 HPV 지식은 낮았고(2.74점), HPV 백신 접종 태도는 긍정적인 편이었다(36.66점). 또한 HPV 백신 접종 의도에 영향을 미친 요인은 출신국가가 베트남인 경우(OR=2.899, p=.048), HPV 백신 교육 요구(OR=5.967, p<.001), HPV 백신 접종 태도(OR=1.083, p=.034)였다. 그러므로 결혼이주여성의 HPV 지식을 높일 수 있도록 교육 프로그램을 개발하여 적용하고, 의료기관이나 대중매체 등 다양한 방법을 통해서 HPV 백신에 대한 정확한 정보를 제공하여 HPV 백신에 대해 긍정적인 태도를 가질 수 있도록 하는 것이 중요할 것이다. 이를 통해 결혼이주여성의 HPV 백신 접종률을 향상시키는데 기여할 것으로 기대된다.

• 디지털융복합연구
• /
• 제19권5호
• /
• pp.263-268
• /
• 2021

본 연구에서는 병원에서 실질적인 태아분만 시스템에 관리를 위한 태아건강분류모델을 설계하는 것을 목적으로 한다. 출산 중 사망자 수는 2017년을 기준으로 295,000명인 산모 사망률과 유사하다. 이러한 사망의 94%는 환경에 의해 발생하므로 대부분 예방할 수 있다. 따라서 본 논문에서는 랜덤 포레스트(Random Forest)를 이용하여 Cardiotocograms(CTG) 검사에서 추출한 2개의 데이터(태아의 심박수, 태아의 움직임, 자궁 수축 등)로 태아의 건강을 예측하는 모델을 제안하였다. 본 연구에서 제안된 모델은 태아분만 보건운영 시스템을 안정적으로 관리하기 위해 태아분만에 대한 데이터의 분포가 불균형한 이상 데이터를 갖는 항목을 찾아 표준편차의 상한 및 하한의 임계값을 설정하여 이상값을 제거하여 정확도를 높혔다. 또한 태아의 건강상태를 나타내는 클래스의 비율이 불규칙함으로, 데이터 리샘플링을 이용하여 소수의 클래스를 복제하여 클래스의 균형을 맞추었다. 그 결과 정확도가 4~5% 향상되어 97.75%로 나타났다. 이에 예측 모델을 통해 발생 할 수 있는 태아의 사망과 병을 사전에 정확히 예측하여 우선적으로 관리함으로써 효율적인 태아 보건운영과 태아 사망 및 병 예방에 기여할 수 있을 것이라고 기대한다.

• 융합정보논문지
• /
• 제11권4호
• /
• pp.194-202
• /
• 2021

부처손 잎은 전통한방에서 탈항, 혈뇨, 부정출혈, 월경불순, 자궁출혈 및 만성간염을 치료 목적으로 사용되어 왔다. 본 연구에서는 부처손을 이용하여 얻은 열수추출물(STW)과 80%에탄올추출물(STE)의 항산화, 항염 및 세포 재생활성을 평가하였다. 그 결과 열수추출물과 에탄올추출물의 폴리페놀 화합물 함량은 각각 38.108±0.766 mg/g, 17.927±1.064 mg/g이었다. DPPH와 ABTS radical 소거능 IC50은 열수추출물이 에탄올추출물에 비해 2배 이상 낮았다. MTT 결과, RAW264.7 세포 생존율은 두 추출물 모두 1 mg/mL에서 6% 감소한 반면, HaCaT 세포의 생존율은 50 ㎍/mL에서 18%로 증가하였다. 열수추출물과 에탄올추출물은 LPS로 자극한 RAW264.7 세포의 NO, TNF-�� 그리고 COX-2, PGE2 등의 염증 매개물질의 생성을 억제하였다. 또한 TNF-a에 의한 각질형성 세포의 손상을 저해시켰다. 이상의 결과로 부처손 열수추출물과 에탄올추출물은 피부건강과 미용 식품을 위한 기능성 원료로 가능성이 높다고 사료된다.

• 대한임상검사과학회지
• /
• 제51권2호
• /
• pp.221-234
• /
• 2019

암은 유전적, 대사질환적 그리고 감염성 질환 등에 의해 유발되는 생명을 위협하는 심각한 질환으로서, 세포의 성장이 정상적으로 통제되지 않으며, 공격적인 형태로 주변의 조직이나 장기로 침범하는 경향을 보이는 생명을 심각하게 위협하는 질병이다. 지난 수십 년 간, 인류의 건강을 위협하는 암을 정복하기 위한 지속적인 노력이 있었고, 암 신생 기전 및 항암제 연구가 항암제 내성에 대한 연구와 함께 다양한 연구주제로 다루어져 왔다. Hinokitiol (${\beta}$-thujaplicin)은 측백나무과 편백속에 속하는 나무에서 분비되는 terpenoid 물질로서, 항염증작용, 항균작용 및 몇몇 암세포 주에서 autophagy를 통한 항암효과가 있는 것으로 잘 알려져 있다. 본 연구에서는, hinokitiol이 세포 영양상태의 변화유무에 관계없이, transcription factor EB (TFEB)의 핵으로의 이동을 촉진한다는 것을 확인하였다. TFEB의 핵으로의 이동은 autophagy 및 lysosome관련 유전자의 발현을 촉진시키고, 세포질 내에 증가된 autosome과 lysosomal puncta의 관찰을 가능하게 하였다. Hinokitiol를 HCC827세포에 처리한 경우에서, 세포 내 autophagy의 증가와 더불어, mitochondria의 hyper-fragmentation과 mitochondria의 authophagic degradation (mitophagy)가 함께 증가되는 것이 관찰되었다. Hinokitiol은 자궁경부암 세포주인 HeLa세포와 비소세포 폐암 세포주인 HCC827에서 암세포 특이 독성을 나타내었다. 더욱이, TFEB 과발현을 통해 autophagy를 인위적으로 증가시킨 HeLa 세포에서 hinokitiol에 대한 세포독성은 더욱 강화된 것으로 나타났다. 이러한 결과들을 통해, hinokitiol은 TFEB의 핵으로의 이동을 촉발시키는 강력한 autophagy inducer임을 확인할 수 있었다. 본 연구에서 처음으로 확인된 hinokitiol에 의한 TFEB의 활성화 및 비소세포성 암세포에서 항암효과의 상승작용은 다양한 항암제 저항성 세포들에 대한 새로운 치료법 및 대체요법 개발과 관련된 의미 있는 결과로 향후, 분자수준의 작용기작에 대한 추가적인 연구가 수행되어야 할 것으로 사료된다.

• 한국동물위생학회지
• /
• 제45권2호
• /
• pp.79-86
• /
• 2022

Reproductive disorders in cows cause economic loss in livestock farms. This study was carried out to investigate the incidence of endometriosis in the uterine of Korean indigenous cow (Hanwoo). In the present study, the uterine of 25 cows was provided by the slaughterhouse. As a result on a visual examination of the uterus, 18 out of 25 were visually normal, and 7 uteruses (28%) appeared rather pale and showed purulent or mucosal symptoms in Uterine horn. However, the results of hematological analysis showed that both RBC and WBC were normal and showed no signs of systemic inflammation, indicating 7 cows showed asymptomatic endometriosis. The inflammatory uterus (28%) showed a wide range of pathological conditions that elicit an inflammatory response, such as serous exudate and bleeding. Histological and microscopic analysis in the inflammatory group demonstrated that there was swelling of the uterine glands, and neutrophil, basophil, and lymphocyte appeared in the uterine gland. Moreover, plasma cells and hemosiderin-laden macrophages were increased in the endometrial stroma, which lead to inhibit pregnancy by suppression of the synthesis of pregnancy hormones, and the appearance of hemosiderin-laden macrophages is an indicator of intracellular bleeding. In summary, hematologically, it is a normal diagnosis in Korean indigenous cows, however, when the uterus was extracted and investigated microscopically, the asymptomatic endometriosis were evident. In order to achieve the goal of healthy cow management and breeding within 2 weeks after birth, cows' uterus should be washed, disinfected, and through thorough the hygiene management, it aims to prevent asymptomatic endometriosis to produce healthy offspring and reduce the breeding interval.

• 대한한방부인과학회지
• /
• 제22권3호
• /
• pp.1-24
• /
• 2009

Purpose: This study was performed to investigate the effects of Bojungikgi-Tang on the development of experimentally-induced endometriosis in rats. Methods: Endometriosis was induced in rats by autotransplanting uterine tissue to the peritoneum and we divided the rats into three groups: (1) sham-operated group (n=8), (2) surgically induced endometriosis and untreated control group(n=8), (3) surgically induced endometriosis and Bojungikgi-Tang treated group. Bojungikgi-Tang was orally administrated for 15 days after operation. Then we measured the body weight, the volume of endometriotic implants, the weight of uterus and ovary, and analysed the concentration of cytokines(MCP-1, TNF-${\alpha}$, IL-1${\beta}$, IL-6) in peritoneal fluids. Histopathology, immunohistochemistry for COX-2 and VEGF, and histochemistry for mast cell in transplanted uterine tissue were also performed. Results: 1. The volume($mm^3$) of endometriotic implants in Bojungikgi-Tang treated group(129${\pm}$39) was significantly decreased(p<0.05) compared with control group(222${\pm}$109). 2. The concentration(pg/$m{\ell}$) of MCP-1 in peritoneal fluids in Bojungikgi-Tang treated group(1012${\pm}$248) was significantly decreased(p<0.05) compared with control group(1413${\pm}$346). 3. The concentration(pg/$m{\ell}$ of TNF-${\alpha}$ in peritoneal fluids in Bojungikgi-Tang treated group(887${\pm}$196) was significantly decreased(p<0.05) compared with control group(1126${\pm}$140). 4. Histopathologically, proliferation of endometriotic epithelia, infiltration of inflammatory cells and angiogenesis in transplanted uterine tissue of Bojungikgi-Tang treated group were weakly observed than those of control group. 5. The percentage of positive epithelial layers for COX-2 in Bojungikgi-Tang treated group(56${\pm}$17) was significantly decreased(p<0.05) compared with control group(75${\pm}$16). 6. The VEGF expression of endometriotic epithelia, neovascular endothelia and stromal cells in transplanted uterine tissue of Bojungikgi-Tang treated group were weakly observed than those of control group. Conclusion: On the basis of these results, we concluded that Bojungikgi-Tang has inhibiting effects on the development of transplanted uterine tissue. And these effects may be related with decreased production of MCP-1, TNF-${\alpha}$, and decreased expression of COX-2 and VEGF infiltration by administration of Bojungikgi-Tang.

• 대한한방부인과학회지
• /
• 제22권4호
• /
• pp.122-134
• /
• 2009

Purpose: The purpose of this study is to know the blood stasis pattern for the patients who had hysterectomy. Methods: Among the patients who had hysterectomy in the department of obstetrics and gynecology, OO medical center from March 3rd 2009 to October 21st 2009, subjects who met inclusion criteria and submitted appropriate questionnaire. We compared blood stasis score and operational method, age, BMI, past operation history of abdominal and pelvic site, parity, whether adhesiolysis, oriental medicine intervention was done. For statistics, we used independent samples t-test, oneway analysis of variances(ANOVA) and SPSS version 13.0 for windows. Results: Blood stasis score of the subtotal hysterectomy group ($44.43{\pm}15.01$) is higher than that of the total hysterectomy group($39.04{\pm}12.46$). Blood stasis score under the age 40($51.88{\pm}12.68$) is higher than 40-49 age patients($40.34{\pm}14.24$) and over the age 50 patients($41.62{\pm}13.46$). There is no statistically significant difference between laparoscopic and abdominal trial, whether adhesiolysis, oriental medicine intervention was done, BMI, past operation history abdominal and pelvic site, parity, chief complaint before hysterectomy, oriental medicine intervention. Conclusion: Through the results showed, when traditional Korean medical doctors use static blood medication, history of hysterectomy and age should be considered as the history of subtotal hysterectomy and younger age tend to have a higher rate of a blood stasis pattern. We need a further study to develop blood stasis indicators with a validated questionnaire, and more subjects involved at multiple institutions.