• Horticultural Science & Technology
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• v.29 no.1
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• pp.1-9
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• 2011

This study was performed to investigate the role of plant growth substances on the bulbing of cold type of garlic (Allium sativum L. cv. Uiseongmaneul) during long and short day conditions. The change in endogenous plant hormones such as abscisic acid (ABA), jasmonic acid (JA) and sugar contents in leaf blade and sheath was examined during the growth stage from bulb differentiation (starting at April 16) to bulbing (April 24 to May 18) in cold type of garlic. In the long day condition, ABA contents were higher than short day condition and ABA contents of leaf sheath were higher than leaf blade. ABA contents of growth stage in garlic were not changed during growth stage from April 16 to May 2, however it rapidly increased during bulbing (May 2 to May 18). On the other hand, endogenous JA contents in short day condition did not change in long day condition, it increased from April 16 to May 2. JA contents in the leaf sheath (33.85-62.04 $ng{\cdot}g^{-1}$ DW) were higher than leaf blade (15.39-30.04 $ng{\cdot}g^{-1}$ DW). These results showed that garlic bulb differentiation and bulbing was induced by JA in leaf sheath. In long day condition, total sugar contents in the leaf blade were increased from bulb differentiation (April 16) to bulbing (May 4) and it was decreased during bulbing (May 4 to May 18) while the total sugar content in leaf sheath were gradually increased from bulb differentiation (April 16) to bulbing (May 18). In conclusion, our results showed that there is a significant correlation between the bulb development of garlic and hormonal content in the leaf sheath.

• Korean journal of applied entomology
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• v.36 no.1
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• pp.88-95
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• 1997

Olfactometer was used to find the attractiveness of kairomone extracted from gypsy moth (Lymantria dispar) to the egg parasitoid (Ooencyrtus kuvanae). The attractiveness of kairomone extracted from scales and hairs which were attached to the surface of the eggs were higher than that of whole eggs with 79.9% and 67.6% respectively. The probing lantency of the egg parasitoid on the extract of scales and hairs were 115.5 sec. and that of whole eggs were 113.2 sec. Eggs taken from egg mass were shown 91.0% of attractiveness to the egg parasitoid, however only 15% of egg parasitoid were attracted by follicles dissected from the host ovary. The probing latency of egg mass was faster than that of follicles dissected from the host with 84.2 sec. and 114.0 sec. respectively. Egg parasitoid showed the most active attractiveness (88.0%), relatively longer examing period (89.2 sec.) and shorter probing latency (26.8 sec.) to the adhesive materials secreted from reproductive accessory glands of the host. Out of six organic solvents used for the extraction of kairomone from the host egg mass, n-hexane fraction received the most active response from xthe egg parasitoid and the parasitoid reared in vivo showed 3 times higher attractiveness to the n-hexane fraction than that of in vitro.

• Horticultural Science & Technology
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• v.29 no.4
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• pp.279-287
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• 2011

This research surveyed the effect of endogenous gibberellins (GA), abscisic acid (ABA), jasmonic acid (JA) and sugar contents on bulb development and secondary growth in the southern type of garlic (Allium sativum L.) cv. Sangdongmaneul and cv. Namdomaneul of Korea. Sangdongmaneul and Namdomaneul showed endogenouse GA, ABA, JA and sugar contents were significantly different in patterns with different cultivar. GA and JA contents of southern type of garlic reached maximum before bulb differentiation and then their contents were decreased. On the other hand, ABA contents gradually increased from bulbing (leaf sheath: 6.58-18.59 $ng{\cdot}g^{-1}$ DW) start. GA contents in Sangdongmaneul and Namdomaneul were not significantly different from each other. These results revealed that GA contents were not affected by secondary growth of garlic. While JA contents (33.0-76.16 $ng{\cdot}g^{-1}$ DW) of Namdomaneul were higher than Sandongmaneul so our results suggest that JA suppressed development of secondary growth of garlic. Total sugar contents of Sandongmaneul and Namdomaneul were not significantly different but total sugar contents were gradually increased after bulb differentiation in Sandongmaneul and Namdomaneul.

• Korean journal of applied entomology
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• v.20 no.1 s.46
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• pp.6-14
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• 1981

The effect of heat or chemotherapeutant treatment on the mosaic virus infected garlic(Allium sativum L.) scales and that of chemotherapeutant added to the culture medium were summarized as following. The treatment of the virus infected garlic scales at $37\~57^{\circ}C$ for 35 days to one hour in water or in air shelved no effort of inactivating the virus. Although treatment of the garlic scales at $62\~72^{\circ}C$ for 90 to five minutes reduced the mosaic symptom on the leaves of the garlic plants grown after the heat treatment, it reduced the growth vigor of the plants so greatly that complete inactivation of the virus in garlics was not feasible. The mosaic symptom on the leaves of garlic plant was reduced when the infected garlic scales were grown after 24 hours soaking in $10\~50\;ppm$ Malachite Green, 2,4-Dichlorophnoxy Acetic Acid, or in $20\~100\;ppm$ Quinhydron. These chemotherapeutants, however, inhibited the growth of garlic plant at the high concentration. Garlic scales soaked in $10\~50\;ppm$ Naphthyl Acetic Acid showed the least mosaic symptom without its complete extiction on the garlic leaves. When incorporated into the modified Murashige-Skoog's medium, $0.5\~l.5\;ppm$ Naphthyl Acetic Acid could inactivate the mosaic virus in newy developed garlic plants showing no mosaic symptom on the leaves, no inclusion bodies and intact nuclei in the leaf tissue cells.

• Korean Journal of Plant Tissue Culture
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• v.28 no.3
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• pp.135-140
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• 2001

A series of studies were carried out to establish micropropagation system, using airlift bioreactors (ebb $\varepsilon$ flood type, 5 L), of Lilium oriental hybrid 'Casa Blanca'. The bulblets with swollen basal plate were formed from bulb scales, then proliferated to bulblet clusters with swollen basal plate. Finally normal bulblets were formed from the sections. Bulblet formation and proliferation with swollen basal plate were not accomplished entirely in liquid culture of 5 L airlift bioreactors, but leafy bulb scales grew vigorously. Bulblet clusters with swollen basal plate were proliferated by periodic immersion culture. Bulblet proliferation was not affected by light, but scale leaves grew under light. MS medium containing 2.0 mg/L benzyl adenine (BA) and 0.3 mg/L indole acetic acid (IAA) was favorable to the bulblet proliferation with swollen basal plate. In liquid culture of 5 L bioreactors, bulblets from bulblet sections with swollen basal plate grew vigorously on MS medium with 70 g/L sucrose. It was effective for bulblet growth to replace the new medium after 8 weeks in culture during 16 weeks of cultural period. 15 g injection of bulblet sections as a cultural material was suitable for bulblet growth in 5 L bioreactors.

• Horticultural Science & Technology
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• v.28 no.5
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• pp.750-756
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• 2010

This study was performed to investigate the role of phytohormones in the bulbing of garlic in order to assess the yield and quality. The effect on endogenous plant hormones such as gibberellin (GA) content was also examined during growth stage i.e. clove differentiation to bulbing in garlic. More than 18 gibberellins in garlic were identified with extensive gas chromatograph-mass spectrometry-selected ion monitoring (GC-MS-SIM) quantitative analysis. The results showed that GAs were biosynthesized by both non C-13 hydroxylation pathway (NCH) and early C-13 hydroxylation pathway (ECH) in garlic plant. It was also revealed that NCH pathway leading to synthesis of bioactive $GA_4$ was the more prominent GA biosynthesis pathway than ECH pathway in which bioactive $GA_1$ was synthesized. Total GAs level was gradually increased from clove differentiation to bulbing and later decreased, which portrays the active role of GA in differentiation. The biosynthesis ratio of bioactive $GA_4$ and $GA_1$ concentration was similar to that of total GAs content, which was closely related with bulb development in garlic.

• Korean Journal of Food Science and Technology
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• v.20 no.2
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• pp.200-204
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• 1988

Cryoprotectivity of garlic bulb caused by the freezing point depression was studied to establish the possibility of preserving the garlic at subzero temperature. Freezing point of fresh garlic tissue showed almost consistency, ranged from $-4^{\circ}C\;to\;-5^{\circ}C$ regardless of the cultivation area. However, the freezing point was varied with the postharvest treatment and storage conditions, so that freezing point of fresh garlic was $-3.5^{\circ}C$ before predrying, $-4.5^{\circ}C$ after predrying, $-5.5^{\circ}C$ after 5 months of storage and that of dead tissue was $-2.5^{\circ}C$. Freezing lethality of fresh garlic bulb preserved at -4, -6.5 and $-15.5^{\circ}C$ were 0, 10 and 70%, respectively. From these results, it was concluded that critical lethal temperature might be $-5{\sim}-6^{\circ}C$. The respiration rate of garlic bulb decreased with lowering the storage temperature down to $-4^{\circ}C$. $Q_{10}$ value was 2 at the temperature range of $-4{\sim}-5^{\circ}C$, 3 at $5{\sim}15^{\circ}C$ and 1.2 at $15{\sim}37^{\circ}C$. In conclusion, optimal temperature for garlic storage was $-4^{\circ}C$ when considering the cryoprotectivity of garlic bulb at subzero temperature.

• Journal of Mushroom
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• v.18 no.2
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• pp.145-150
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• 2020

A new cultivar, 'Bambithyang', was bred from monokaryotic strains of Sanbaekhyang and SANJO 707ho using the mono-mono hybridization method. Its incubation period was determined to be 100 days and its optimum temperature for fruit body flushing, 11-20℃. The mushrooms had a convex cap (diameter, 69.6 mm; thickness, 15.2 mm) and stipes in their center. The color of their upper sides was chestnut brown; their gills were arranged in a ripple style and had medium density. Their scales were cream-colored and fully distributed; the stipes were cream-colored and cylindrical (thick upper part). They also had cream colored fluffs and sporadic fruiting bodies. Zone lines were present when they were cultivated with mother and father varieties. Their stipe lengths and caps were 9% shorter and 16.9% thicker, respectively, than those of Sanbaekhyang.

• (The)Study of the Eastern Classic
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• no.69
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• pp.419-450
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• 2017

This study aims to discover the clue of the current modern postal system development by looking at the delivery patterns of Korean letters during the Joseon Dynasty. A letter is a text medium that the sender sends to a recipient by sending a message of news. The form and contents of the letter are influenced by the various surrounding factors and are adapted to circumstances. The study aims to clear up the method of delivery and the nature of the transmitter among the environmental factors which affects the format and contents of Korean letters. Depending on the public or private matters, the delivery method is divided into a public agency and a private messenger, or divided into one-phase delivery and the multi-phase delivery depending on whether or not a phase is divided into stages. It is also divided into a one-way and a round-transmission depending on whether or not it is round-switched. The transmitter is divided into a verbal communication, the letter and verbal communication, and the letter communication according to whether or not it is by verbal, and divided into the relational and the non-relational person according to the relationship with the sender. Also depending on whether the transmitter is exclusive or not, it is divided into the exclusive messenger and the messenger who deliver a letter incidentally while come and go. In the aspect of the delivery method, We can see the possibility of developing to a modern mail system through the public method, the multi-phase delivery, one-way delivery and in the aspect of the transmitter, we can see the possibility through the letter delivery, non-related transmitter, and the exclusive messenger.

• FLOWER RESEARCH JOURNAL
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• v.16 no.3
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• pp.211-217
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• 2008

To build up data for mutation breeding of lily, sensitivity to irradiation of bulb, seed and pollen was investigated. The bulbs of seven cultivars including 'New Wave' were very sensitive to gamma rays. Non-irradiated bulbs produced plants with bloom as normal. However, irradiated bulbs showed retarded growth and did not flower. Bulbs of 'New Wave' and 'Tiny Dino' irradiated with 125Gy gamma rays survived, but their growth rate dropped considerably. Part or whole leaves of the other cultivars except 'New Wave' and 'Tiny Dino' withered. Moisture content of individual scale was 72~78% depending on cultivar. Bulblet formation from untreated scales was 100%. Among all irradiated scales only 'Siberia' scales showed 8.5% bulblet formation when irradiated with 50Gy gamma rays. Pollen germination as affected by gamma ray irradiation had no consistent tendency. When dosage of $F_1$ 'Augusta' seeds reached 300Gy, their survival ratio was drastically decreased. Bulblet formation in vitro was observed only in non-irradiated seeds. Lethal dose ($LD_{50}$) of seed was less than 100Gy. Height and width of $F_1$ 'Augusta' bulblets in vitro irradiated with gamma rays decreased as dosage increased regardless of the culture method. The percentage shoot formation was 100% in non-irradiated bulblets and 15~60% in irradiated ones.