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가공반찬류의 저장성 향상을 위한 천연 항균복합제재의 처리효과

  • 정준호;조성환
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 2003.10a
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    • pp.190-191
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    • 2003
  • 식품조리 및 가공은 만들어진 제품의 저장성을 전제로 해야 하기 때문에 식품의 변화 혹은 변질에 깊은 이해를 바탕으로 모든 처리와 가공 방법이 검토되어야 한다. 식품의 변질이나 부패는 미생물 작용에 의한 변질이 그대부분을 차지하고 있으며 미생물학적 변질을 방지하기 위해서 화학적 합성보존 제가 상업적으로 사용되고 있으나 최근 소비자의 건강 지향적 욕구가 증대됨에 따라 인공합성품의 기피현상이 두드러지고 있다. 따라서 천연 항균물질의 개발과 이용은 인공 합성보존제의 대처라는 의미와 소비자기피현상을 유발시키지 않으면서 각종 가공식품의 저장성 향상 및 저온 유통식품의 안정성확보라는 견지에서 그 중요성이 있다. 본 실험에서는 식품의 미생물작용에 의한 변질의 저해 효과가 뛰어난 식물성천연항균제품(Botanical antimicrobial agents-Citrus product : 이하 BAAC라 칭함)에 천연보조제(Ginseng extract, Aloe, 매실추출물)를 첨가하여 Paper disk법에 의한 항균성 검사를 실시한 결과 Ginseng extract를 첨가한 경우 생육저해환이 가장 크고 뚜렷하게 나타났다. 따라서 BAAC에 보조제 (Ginseng extract)를 첨가하여 제조 된 천연 항균복합제재(이하 BAAG라 칭함)를 가공 반찬류인 마늘종 무침과 돈육장조림에 첨가하여 처리한 후 꺼내어 상온에서 저장하면서 주사전자현미경 관찰, 대장균수, 총균수의 변화, 외관상의 변화에 미치는 영향을 무처리 대조구와 비교하면서 조사하였다. 주사전자현미경 촬영사진의 결과 분석을 통하여 BAAG를 처리한 균체 세포는 세포막 및 세포벽 기능이 파괴되어 세포내용물이 균체외부로 유출되어 균체의 생육이 억제되며 성장을 저해 또는 사멸시키는 것으로 나타났다. 대장균수는 모든 처리구에서 BAAG의 첨가에 의해 성장이 억제되었으며 농도가 증가시킬수록 균증식 억제 효과가 뚜렷하였으며, 총균수도 압도적으로 낮은 값을 보여주었다. 외관상의 변화와 상품 가치를 측정한 결과는 무늘 종 무침과 돈육장조림의 대조구에서는 4일 경과후 강한 부패취와 아울러, 표피의 갈변정도가 심하되어 관능적으로 부패상태를 인지할 수 있었으며 7일경과 후부터 점질성 갈변물질이 생성되어 상품가치가 크게 떨어진 상태였다. 이와 같은 결과로 볼 때, BAAG의 처리는 BAAC의 경우보다 가격은 저렴하면서도 항균력은 우수한 천연 항균복합제재로써 가공 반찬류의 저장성을 최대한 연장 할 수 있음을 확인 하였고, 반찬류뿐만 아니라 여러 식품군에 다양하게 적용가능 할 것으로 기대된다.

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Short-term Effects of Cultivars and Compost on Soil Microbial Activities and Diversities in Red Pepper Field (토양 미생물 활성과 다양성에 미치는 고추 품종과 퇴비의 단기적 효과)

  • Park, Kee-Choon;Kwon, Tae-Ryong;Jang, Kil-Soo;Kim, Yeong-Suk
    • Korean Journal of Environmental Agriculture
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    • v.27 no.2
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    • pp.139-144
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    • 2008
  • A field experiment was conducted to investigate the influence of cultivars and compost on soil microbial activities and diversities in a red pepper-grown field. Compost was applied with 0, 30, and 60M/T $ha^{-1}$ in April and then red pepper seedlings of "Yong-go 4" and "Koeun" were transplanted in May 2007. Soil samples were collected in early August 2007. Measurement of microbial activities was based on a dehydrogenase assay and a fluorescein diacetate hydrolysis. Soil microbial community was characterized with Biolog $EcoPlate^{TM}$ and phospholipid fatty acid(PLFA). Red pepper cultivars did not differentiate the selected soil chemical and microbial properties. Soil pH and soil microbial community changed by amending the soil with 30 and 60 M/T $ha^{-1}$ of compost, and the soil organic matter and potassium content, and soil microbial activities increased in soils amended with 60 M/T $ha^{-1}$ of compost. Red pepper cultivar induced a little different soil chemical properties and microbial activity in soils amended with 60 M/T $ha^{-1}$ of compost even though significant differences were not found in those properties. In conclusion the effects of compost on soil chemical and microbial properties were much higher than red pepper cultivars in short-term period but the effects of red pepper cultivars should be investigated in long-term field test.

Detection of a Quorum-Sensing Inhibitor from the Natural Products (천연물로부터 Quorum Sensing 저해제의 탐색)

  • Kim, Tae-Woo;Cha, Ji-Young;Lee, Jun-Seung;Min, Bok-Kee;Baik, Hyung-Suk
    • Journal of Life Science
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    • v.18 no.2
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    • pp.206-212
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    • 2008
  • The quorum sensing (QS) regulatory network has been the subject of extensive studies during recent years and has also attracted a lot of attention because it both positively and negatively regulates various putative virulence factors, although initially considered to be a specialized system of Vibrio fischeri and related species. In this study, to identify the novel materials which inhibit QS system of microorganisms, extracts of eighteen natural products were tested by bioassay using N-(3-oxohexanoyl)-$_L$-homoserine lactone and N-(3-oxooctanoyl)-$_L$-homoserine lactone synthesized in this experiment and an Agrobacterium tumefaciens NT1 biosensor strain containing a traI::lacZ fusion. The result indicated that the extracts of cabbage, leek, and onion exhibited the QS inhibition activity. Thus, materials contained in the extracts were isolated via recycling preparative HPLC and were purified via a JAIGEL-LS255 column. The common fraction corresponding to a peak of the 83 min point of them quenched the quorum sensing of A. tumefaciens NT1 biosensor strain in ABMM containing X-gal and was designated quorum sensing inhibitor-83 min (QSI-83). The QSI-83 exhibited the heat stability and did not inhibit the growth of A. tumefaciens NTl. Furthermore, thin layer chromatography (TLC) results suggested that these novel materials may be antagonists of N-acyl homoserine lactone or may inhibit the QS autoinducer synthesis by Pseudomonas syringae pv. tabaci.

Job Titles of Medical Technologist Managers in a Hierarchical System and Team System (계층제와 팀제에서 임상병리사 관리자 명칭)

  • Koo, Bon-Kyeong
    • Korean Journal of Clinical Laboratory Science
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    • v.50 no.1
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    • pp.54-62
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    • 2018
  • In the 2000's, due to a change in hospital management strategy, the organizational structure shifted from a hierarchical system to a team system. While the hierarchical system is characterized by being activity centered, job title linked, and vertically managed, the team system is characterized by being competency centered, job title segregated, and horizontally managed. The job titles of medical technologist manager was surveyed three times in 1997, 2007, and 2017. It has been confirmed through staff members working at 24 hospitals in more than 500 beds in the metropolitan area. The results of job titles follow are as follow: "Team Leader; Part Leader" 14/24 (59%), "Chief Technologist; Area Head Technologist" 7/24 (29%), and "Chief" 3/24 (12%). The present authors propose an alternative name based on the team system to refine the three job titles currently used by medical technologists. First, the Chief Technologist is unclear if it refers to the Technologist General Manager or Technologist Manager. The Chief Technologist should be changed to "Team Leader". Second, given that Area Head Technologist or Section Chief are on the same position as Head Nurse, we suggest that Area Head Technologist or Section Chief should be changed to "Part Leader". Third, while the organization regulation is marked merely as Department of Laboratory Medicine according to the hierarchical system, it is marked as Laboratory Medicine Team according to the team system. Medical technologists come to have more belongingness, feeling of solidarity, and intimacy under the team system.

Citizens' Consciousness on Urban Changes in Large Cities in the 1990s(2) : A Comparative Study on Spatial Environment and Urban Policy (1990년대 대도시의 변화에 관한 시민 의식 연구(2) : 공간환경 및 도시정책 비교)

  • Choi, Byung-Doo;Kim, Soon-Cheon;Hong, Myung-Pyo
    • Journal of the Korean association of regional geographers
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    • v.10 no.2
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    • pp.447-465
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    • 2004
  • This paper is to analyze citizens' consciousness on urban changes, especially focusing on changes in urban environment, spatial structure, and urban planning, of large cities in S. Korea, that is, Seoul, Daejeon, Daegu, and Kwangju in the 1990s. Some significant points can be described as follows. First. The most important factor influencing on the change of urban spatial structure was the expansion of residential area inner and outside of cities promoted by the traditional construction industry, and not development of high-tech industries and their complex or location of multi-national firms which can be emphasized as major factors of new urbanization process of global cities. Secondly, citizens seem to have experienced improvement and deterioration of urban environment simultaneously, emphasizing regulatory environmental policy on the one hand, and recognize the necessity of sustainable urban development within given environmental capacity and restoration of destructed and polluted environment of cities. Thirdly, the building boom of new apartment complex was a major driving force of changing urban spatial structure, but it could neither alleviate a residential gap between the poor and the rich areas nor a relief of traffic congestion. Finally, the urban policy in general was felt somewhat improved by citizens, but not in sectoral details.

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Cellular Protective Effect and Liposome Formulation for Enhanced Transdermal Delivery of Isoquercitrin (Isoquercitrin의 세포 보호 작용과 피부 흡수 증진을 위한 리포좀 제형 연구)

  • Jo, Na-Rae;Gu, Hyun-A;Park, Su-Ah;Han, Seat-Byeol;Park, Soo-Nam
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.38 no.2
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    • pp.103-118
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    • 2012
  • In this study, the cellular protective effect of isoquercitrin against $H_2O_2$ and rose bengal-indued HaCaT cell damage was investigated. The ethosome and elastic liposome for enhanced transdermal delivery were prepared. Particle size, loading efficiency and cumulative permeated amounts of them were evaluated. Isoquercitrin didn't show any characteristic cytotoxicity at 50 ${\mu}M$. When HaCaT cells were treated with 5 mM $H_2O_2$ and 25 ${\mu}M$ rose bengal, isoquercitrin protected the cells against the oxidative damage in a concentration dependent manner (6.25 ~ 50 ${\mu}M$). The size of 0.03 % isoquercitrin loaded ethosome was 222.85 nm and the loading efficiency was 82.26 %. The ethosome loaded with 0.03 % isoquercitrin was stable and maintained the constant particle size for 2 weeks after being prepared. The ethosome exhibited more enhanced skin permeability than general liposome and ethanol solution. The optimal ratio of lipid to surfactant of 0.1 % isoquercitrin loaded elastic liposomes was observed to be 89 : 5 through evaluating particle size (341.2 nm), deformability index (59.89), loading efficiency (54.3 %), and skin permeability (54.4 %).

Recovery of a High Molecular Soluble Protein from Surimi Wastewater Using Calcium Powder of Cuttle Bone (갑오징어갑 칼슘을 이용한 Surimi 가공폐수로부터 단백질의 회수)

  • KIM Jin-Soo;CHO Moon-Lae;HEU Min-Soo;CHOI Yeung Joon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.2
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    • pp.80-87
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    • 2003
  • Recovery conditions and characteristics of a high molecular soluble protein from surimi processing wastewater in marine manufacture using calcium powder of cuttle bone treated with acetic acid (ATC) were examined. Judging from results of total-N, pH, COD, turbidity and yields, optimal treatment concentration of ATC for recovery of high molecular soluble proteins from wastewater was $1.0\%.$ The protein recovered from seafood waste (PRW) was macromolecule weight. The COD value in the wastewater treated with ATC was very high. The PRW had a $78.4\%$ in moisture, $1.0\%$ in crude lipid and $5.7\%$ in crude ash. The proximate composition, except the crude ash, of the PRW was similar to that of commercial surimi. The PRW showed white index and similar in the content and in the composition of total amino acid to those of commercial surimi. From the results of sensory evaluation on white index and texture, the heat-induced surimi gel prepared with $5\%$ subsititution of the PRW for bulking agent of commercial surimi was not significantly different compared to that prepared with the original commercial surimi.

Problem Solver's Responses According to the Sentence Structures of Mathematical Word Problems (수학 문장제의 문장 구조에 따른 초등학생의 문제해결 반응 비교 분석)

  • Kang, Wha-Na;Paik, Suck-Yoon
    • Journal of Educational Research in Mathematics
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    • v.19 no.1
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    • pp.63-80
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    • 2009
  • This paper has a purpose to find out the important points about linguistic factors suited to the assessment purpose and mathematics teaching/learning that a word-problem sentence has to possess. We also examine the degree of understanding of sentence and the perceptive/emotional reactions of students toward two different kinds of word-problem sentences that have same mathematical contents, but different linguistic structures. The objects of this thesis are 124 students from the third to sixth grade in an elementary school. We execute assessment of simple-sentence-word-problem and complex-sentence-word-problem that have same mathematical contexts, but different linguistic structures. Then we have compared and examined their own process of solving the two types word-problems and we make up questionnaire and have an interview with them. The conclusions are as followings: First, simple-sentence-word-problem is more successful to suggest an information for solving a problem than complex one. Second, it is hard to find the strategy for solving a problem in complex-sentence-word-problem than simple one. Third, students think that suggested information and mathematical knowledge are different according to the linguistic structure in the process of perceiving the information after reading a word-problem. Fourth, in spite of same sentence type, the negative mental reaction is showed greatly to complex-sentence-word-problem even before solving a problem.

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Parents' Recognitions and Attitudes on Identification of Gifted Students Using Observation and Nomination by Teachers in Busan (교사 관찰.추천제를 활용한 영재교육 대상자 선발방식에 대한 부산지역 초등학교 학부모의 인식과 태도)

  • Choe, Ho-Seong;Park, Hoo-Hwi;Kim, Eel
    • Journal of Gifted/Talented Education
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    • v.21 no.2
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    • pp.407-426
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    • 2011
  • This study is to examine the perceptions and attitudes of parents about the teachers' recommendation system through their observations to identify the gifted. We conducted surveys for parents who had elementary school children in Busan metropolitan city and analysed the results to find out about the differences among parents groups in terms of their monthly incomes and opinions on teachers' observation and nomination for the gifted. The results are following; First, approx. 80% of the respondents recognized the changes in the giftedness identification system. Also, 40.5% of the parents positively thought about the teachers' observation and nomination system (positive group), whereas 32.3% of the parents showed their negative opinions about the system (negative group). There were also 27.2% of the parents who did not make their decisions (no-decision-making group). Second, most of the parents accepted that the teachers who were professionally trained in gifted education should be the persons for observing and nominating gifted students. However, significant differences were shown among the different monthly income groups of the parents in regards to who should be the person taking charge of recommending the gifted and how trustworthy this person could be. Third, the positive parents' group mostly expected that as the teachers' observation and nomination system is adopted, expenses for private education would decrease, whereas the negative group and no-decision-making group thought that the opposite result would occur. These results will be helpful for the successful adoption of teachers' observation and recommendation system to identify the gifted in the field of education.

Policy Implications for the Success of a Trekking Time Restriction Policy in National Parks (국립공원 입산시간지정제 정착을 위한 정책 제언)

  • Cho, Woo;Sung, Chan Yong
    • Korean Journal of Environment and Ecology
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    • v.29 no.4
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    • pp.636-644
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    • 2015
  • In this paper, we examined factors that affect the success of a policy on trekking time restriction using questionnaire survey data collected from the visitors of seven national parks where the trekking time restriction policy is in force as of 2014. Results suggest that the trekking time restriction policy has been successfully publicized. In total, 60.7% of the survey respondents were aware of trekking time restrictions in the national parks they were visiting. Using exploratory factor analysis, we identified three latent factors (visitors' careless trekking, park rangers' insufficient management and visitors' unpreparedness) that the visitors perceived the causes of trekking accidents in the national parks. Multiple regression analysis on the three extracted factors and respondents' socioeconomic status shows that the respondents who read information signs in national parks and who judged visitors' careless trekking and visitors' unpreparedness as the causes of trekking accidents tended to agree more with restricting trekking time. These results indicate that visitors who do not agree with the trekking time restriction tend to attribute trekking accidents in national parks not to individual visitors, suggesting that educating visitors is more effective in preventing trekking accidents in national parks than installing and maintaining safety facilities by park rangers.