• Journal of Life Science
• /
• v.24 no.9
• /
• pp.1025-1029
• /
• 2014

Cervical carcinoma is the second leading cause of cancer-related deaths in women around the world, and it is associated with the Human Papillomavirus (HPV) infection. HPV genotyping is important for vaccine policy, etiology, natural history, and epidemiology studies. The use of formalin-fixed paraffin-embedded (FFPE) tissues for HPV genotyping by reverse blot hybridization assays (REBA) has not been clearly confirmed in retrospective studies. The aim of this study was to evaluate the usefulness and efficiency of FFPE tissues from cervical cancers for HPV genotyping. HPV genotypes were detected in 52 FFPE tissues from cervical carcinoma specimens by REBA. HPV was detected in 32 (61.5%) of 52 specimens from FFPE, among which 27 (84.4%) harbored single infections and 5(15.6%) contained multiple infections. The HPV single infections (27) were analyzed by high-risk type 18(8), 58(6), 16(5), 33(1), 35(1), 39(1), 56(1) and low risk type 11(2), 6(1), 70(1). The HPV multiple infections (5) included 16/18(2), 18/52(1), 16/56(1), 16/18/33(1). Please consider being more specific here. Do you mean the analysis? Please clarify what you mean by "included."Through this study, it has been determined that the FFPE specimen is feasible and can be used in HPV genotyping, as well as in retrospective studies.

• Journal of Life Science
• /
• v.21 no.7
• /
• pp.947-952
• /
• 2011

A number of epidemiological studies have identified human papillomavirus (HPV) types 1, 2, 3, 4, 7, 10, 27, 57, and 65 in cutaneous common warts. However, identification of the HPV subtype by conventional polymerase chain reaction (PCR) is time consuming with its multi-step laboratory process. In this study, we aim to develop a specific one-step multiplex polymerase chain reaction method which capably identifies six different HPV genotypes related to common warts. By HPV DNA sequence analysis, 6 pairs of specific primers were designed from the intergenic regions of genes L1 to E6, and from genes E2 to L2. DNA sequence analysis with the L1 gene sequence of the sample was performed to measure the specificity of multiplex PCR. HPV-1, -2, -3, -4, -27, and -57 were identified without cross amplification in 109 out of 129 samples. The sensitivity and specificity of our set of primers in detecting HPV were 85% and 99.5%, respectively. For the 20 samples where HPV type was not identifiable by our batch of primer sets, multiplex PCR with an additional set of HPV primers was done, where 7 were found positive for HPV-7 or -65. Our results demonstrate that the newly designed multiplex PCR can rapidly detect the specific HPV subtype involved in common warts with high accuracy.

• Proceedings of the Korean Information Science Society Conference
• /
• 2003.04c
• /
• pp.401-403
• /
• 2003

인유두종 바이러스는 표피세포에 감염되는 DNA 바이러스로 자궁경부암을 일으키는 가장 큰 요인이다. 현재까지 100 여개의 종류가 알려져 있고 악성종양 유발 가능성에 따라 위험군을 나누는데. 여기서 중요한 것은 고위험군을 저위험군으로 잘못 분류하는 것을 최소화하는 것이다. 본 논문에서는 분류를 위한 데이터로 인유두종 바이러스에 관한 문서 자료들을, 기계 학습 방법으로 분류 비용을 고려해 줄 수 있는 비용 의존 학습을 이용하였다. 실험결과. 비용을 고려해 주는 것이 고려하지 않았을 때보다 더 종은 성능을 나타내었다.

• Journal of Digital Convergence
• /
• v.13 no.12
• /
• pp.235-244
• /
• 2015

This study was done to identify convergence factors influencing the HPV vaccination in female university students. The subjects of this study were 546 female university students. The data were collected through interviews using a structured questionnaire from 5 to 30th of October, 2013. 138(25.3%) reported that they were vaccinated. Religion, ever heard cervical cancer, HPV and cervical cancer vaccination, ever had a pap test, knowledge about HPV vaccination, health beliefs(total), perceived benefit, perceived barrier, cancer preventive behavior(total) and cervical cancer preventive behavior were associated with being vaccinated. In logistic regression analysis showed that the predictors are ever had a pap test(OR=34.67, 95% CI=17.19~ 69.92), perceived benefit(OR=1.45, 95% CI=1.17~1.81) and perceived barrier(OR=1.44, 95% CI=1.27~1.63). It was recommended to make convergence policy supports and education programs reinforcing a pap test and perceived benefit and reducing perceived barriers about HPV vaccination.

• The Journal of the Korea Contents Association
• /
• v.19 no.4
• /
• pp.381-393
• /
• 2019

This study aimed to develop an educational program on cervical cancer prevention and human papillomavirus (HPV) for female North Korean refugees and to assess the effect of the program. This study used a nonequivalent control group with a pretest-posttest design. The participants were 42 female North Korean refugees in their 20s residing in South Korea. Measurements included cervical cancer knowledge, HPV knowledge, attitude toward HPV vaccination, and intention for HPV vaccination. Statistically significant differences emerged in levels of cervical cancer knowledge (U = 40.00, p < .001), HPV knowledge (U = 4.50, p < .001), and attitude toward HPV vaccination (U = 128.00, p = .013) between the experimental and control group. The educational program is an effective nursing intervention to improve cervical cancer knowledge, HPV knowledge, and attitude toward HPV vaccination of female North Korean refugees. Stakeholders must work to establish health policies to reduce health disparities between South and North Korean women to prepare for unification.

• Women's Health Nursing
• /
• v.26 no.1
• /
• pp.37-48
• /
• 2020

Purpose: The purpose of this study was to identify the factors influencing mothers' intention to vaccinate their elementary-school sons against human papillomavirus (HPV). Methods: The participants were 151 mothers of fourth- to sixth-grade boys at three elementary schools in Daejeon, Korea. The data were analyzed using SPSS for Windows version 25.0. Descriptive statistics, the independent t-test, one-way analysis of variance, the Mann-Whitney U test, Pearson correlation coefficients, and multiple regression analysis were used. Results: The mothers' score for intention to vaccinate their sons against HPV was 5.04 out of 7. Self-efficacy (β=.60, p<.001) and subjective norms towards HPV vaccination (β=.30, p<.001) were significant factors influencing mothers' intention to vaccinate their elementary-school sons against HPV. These factors accounted for 81.0% of HPV vaccination intention among mothers (F=160.84, p<.001). Conclusion: The results of this study suggest that to increase the HPV vaccination rate for elementary-school boys, it is necessary to develop intervention strategies to improve mothers' self-efficacy and subjective norms towards HPV vaccination and to verify the effects of those strategies. This research provides a foundation for designing interventions to increase the HPV vaccination rate of elementary-school boys.

• The Journal of Korean Academic Society of Nursing Education
• /
• v.16 no.2
• /
• pp.257-264
• /
• 2010

Purpose: This research was conducted to determine the effects on HPV knowledge, cervical cancer preventive behaviors, and sexual autonomy of female university students after they received sexual education to prevent HPV involved cervical cancer. Method: By using a non-equivalent control group pretest and posttest design, the experiment was conducted with a test group of 37 students and a control group of 39 students. Result: There were statistically significant increases in HPV knowledge (p=.003), cervical cancer preventive behaviors (p=.001), and sexual autonomy (p=.005) in the experimental group compared to the control group. Conclusion: It is concluded that this research is valid because this research program has proved to be effective in increasing HPV knowledge, cervical cancer preventive behaviors, and sexual autonomy, and it has provided a foundation to be used with other female university students.

• Korean Parent-Child Health Journal
• /
• v.20 no.1
• /
• pp.39-47
• /
• 2017

Purpose: This study was conducted to investigate the factors predicting HPV (Human Papilloma Virus) vaccination practices among female college students. Methods: A convenience sample of 207 female students attending four universities in one metropolitan city participated. Self-report questionnaires consisted of general characteristics, characteristics related prevention of cervical cancer, knowledge of HPV, knowledge of cervical cancer vaccination, and health beliefs related to HPV vaccination. Data were analyzed by $x^2$ test, independent t-test, and bivariate logistic regression. Results: Factors predicting HPV vaccination practices were information about HPV (OR=3.37), experience of HPV test (OR=12.71), and health beliefs related to HPV vaccination (OR=1.13). Conclusion: In order to increase the practice rate of HPV vaccination, it is necessary to provide simple key information that is easy to understand, rather than expert knowledge about HPV. Therefore, it is necessary to provide a way for college students to get information about virus easily. It is necessary to intervene integrally with the facilitation factor and obstacle factor of vaccination practice.

• Women's Health Nursing
• /
• v.20 no.1
• /
• pp.105-114
• /
• 2014

Purpose: The goal of this study was to examine knowledge level of human papillomavirus (HPV), cervical cancer, and vaccination status among Korean mothers' with a daughter in high school. Methods: A cross-sectional descriptive study was conducted with a convenience sample of 234 Korean mothers. Results: The mean score of HPV knowledge level was 4.21 out of 20, and cervical cancer knowledge was 3.88 out of 7. There were low levels of daughters' who received HPV vaccination (3.8%) and high levels of mothers' intention to get their daughters' vaccinated with HPV (85.0%). The major barrier to HPV vaccination was the concern for side effect from the vaccination. The most effective recommendation for HPV vaccination came from health care providers. Conclusion: HPV educational programs targeting mothers of daughters in high school is needed. Future studies need to determine actual HPV vaccination rates and factors related to high uptake using a larger sample from various socioeconomic background.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.15 no.7
• /
• pp.4212-4219
• /
• 2014

Purpose: The goal of this study is to assess the factors influencing the HPV vaccination of females in their twenties. Methods: The survey was conducted during 2013.11.11-28 with a convenience sample of 488 participants. Results: The mean score of their knowledge about cervical cancer shows 2.54 out of 7. 96.7% and 31.5% of respondents have heard about cervical cancer and HPV, respectively. Only 2.7% are vaccinated by the recommendations from the medical teams. The higher the knowledge scores, the more the subjects are vaccinated. The factors influencing HPV vaccination appear to be prior knowledge about the HPV. Conclusion: The knowledge level is low compared to previous studies. Despite this, the trend of vaccination rate is higher. Medical teams need to promote vaccination. Accordingly, education and promotion are needed for the unvaccinated.