• Title/Summary/Keyword: 인공 광 조건

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Propagation Condition for Sporophyte Mass Production of Loxogramme salicifolia (Makino) Makino (버들일엽 포자체 대량생산을 위한 번식조건)

  • Jang, Bo Kook;Lee, Ki Cheol;Lee, Cheol Hee
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.10a
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    • pp.40-40
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    • 2018
  • 비고사리과(Lindsaeaceae) 버들일엽[Laxqgramme saliciblia (Makino) Makino]은 잎이 녹색의 단엽으로 버들처럼 보여 관상가치가 높아 조경소재로 이용되는 착생식물이다. 또한 식물구계학적 특정식물종 V급, 적색자료집 VU급, 국외반출 승인대상종에 수록된 식물로 생태학적 보전이 요구되며, 개체수도 적은 편으로 알려져 있다. 본 연구는 보전이 요구되는 버들일엽의 전엽체 증식 및 포자체 형성을 위한 기내 외 번식조건을 구명하고자 수행되었다. 서귀포시 상효동 일대에서 식물을 획득하였으며, 기내에서 포자를 발아시켜 획득한 전엽체를 8주 간격으로 계대배양하면서 실험재료를 확보하였다. 전엽체 증식에 적합한 배양배지를 선발하고자, 전엽체 300mg을 다진 후 배지종류와 농도를 달리한 배지(Knop, 1/4, 1/2 및 1MS)에 8주간 배양하였다. 배양실은 온도 $25{\pm}1.0^{\circ}C$, 광도 $30{\pm}1.0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$(16/8h)로 조절되었다. 연구의 결과, 1/2MS배지에서 전엽체의 생체중이 5.8g으로 가장 많이 증가하였으며, 형태형성의 발달도 주걱형의 전엽체로 정상적으로 발달하였다. 기외 포자체 형성에 적합한 토양조건을 확인하고자, 인공토양(원예상토, 피트모스, 펄라이트 및 마사토)의 비율을 달리하여 5종류의 혼합토양을 조성하였다. 준비된 토양을 사각분($7.5{\times}7.5{\times}7.5cm$)에 충진하고, 전엽체 1g을 10초간 분쇄한 다음 토양표면에 분주하여 16주간 재배하였다. 재배환경은 온도 $25{\pm}1.0^{\circ}C$, 광도 $43{\pm}2.0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$(16/8h) 및 습도 $72{\pm}2.0%$로 조절되었다. 연구의 결과, 원예상토가 많은 비율로 혼합된 처리에서 포자체의 형성이 확인되었다. 원예상토와 펄라이트를 2:1(v:v)로 혼용한 토양, 원예상토와 마사토를 2:1(v:v)로 혼용한 토양, 원예상토 단용한 토양에서 각 28.5, 23.0, 22.3개로 조사되었다.

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Conditions for Artificial Culture of Lemna Paucicostata and Potentiality as an Alternative Biomass Source (바이오매스 자원으로서의 Lemna Paucicostata의 인공배양조건과 이화학적 특성에 관한 연구)

  • Kwag, Jung-Hoon;Lee, Jin-Eui;Kim, Ki-Hye;Eum, Hye-Yeong;Shin, Jong-Suh;Ra, Chang-Six
    • Journal of Animal Environmental Science
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    • v.16 no.2
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    • pp.143-152
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    • 2010
  • Conditions for artificial culture of Lemna Paucicostata and its nutritional values were examined in this study. Lemna P. was cultured using artificial wastewater and a bioreactor (total volume $2,630\;cm^3$, working volume $2,240\;cm^3$) was operated at conditions of 6,250 lux and $28^{\circ}C$. Water flow affected the growth of Lemna P.: growth rate was very high (more than $1.1\;d^{-1}$) at a condition of no-water movement, but it was very low (less than $0.15\;d^{-1}$) when water moved slowly. The growth of Lemna P. was higher in $16h\;d^{-1}$ light cycle than in Sand $24h\;d^{-1}$, and it was also severely affected by the initial $NH_4$-N levels of wastewater. The growth rate of Lemna P. was high in lower $NH_4$-N level, indicating that the growth rate is in inverse proportion to $NH_4$-N concentration in wastewater. However, the contents of crude protein (CP) of Lemna P. were proportional to the initial $NH_4$-N concentration. The CP contents of Lemna P. cultured at 2, 10, 50 and 100 $NH_4$-N mg $L^{-1}$ was 18, 24, 37, 43%, respectively, showing the Lemna P. cultured at 50 and $100\;mg\;L^{-1}$ had similar protein contents to linseed (CP 35%), cottonseed (CP 38%) and soybean (CP 45%). Fat, protein, fiber, NDF and ADF contents of Lemna P. harvested at conditions of $16h\;d^{-1}$ light cycle and less than $2\;mg\;L^{-1}$ of $NH_4$-N level was 2.8, 18, 27, 20, 41 and 65.7%, respectively. Since the growth rate of Lemna P. was very high (more than $1.1\;d^{-1}$) at those conditions, it was convinced that mass production of valuable protein and fiber sources are feasible. In particular, since the Lemna P. has unsaturated fatty acids found mainly in animal fat as well as beneficial fatty acids to health such as C18:ln9c, C18:2n6c, C20:5n3 and C22:2, the Lemna P. biomass would be a highly valuable alternative feed source to grains.

OPTICAL PROPERTY AND ALIGNMENT OF KAO WIDE FIELD TELESCOPE (NEOPAT-3) (광시야 망원경 3호기 (NEOPAT-3)의 광학계 특성 및 조정)

  • Yuk, In-Soo;Kyeong, Jae-Mann;Yoon, Joh-Na;Yoon, Jae-Hyuck;Yim, Hong-Suh;Moon, Hong-Kyu;Han, Won-Yong;Byun, Yon-Ik;Kang, Yong-Woo;Yu, Sung-Yeol
    • Journal of Astronomy and Space Sciences
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    • v.21 no.4
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    • pp.417-428
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    • 2004
  • We have investigated the optical property of the KAO(Korea Astronomy Observatory) wide field telescope (named NEOPAT-3; Near Earth Object and Satellite Patrol-3) and aligned optical system. The NEOPAT-3 is restricted to V,R,I-filters because of the refractive property of the correcting lens system. Because of the fast focal ratio, the optical performance of the NEOPAT-3 is very sensitive to its alignment factors of the optical system. To make the spot radius smaller than $8{\mu}m$ in rms over 2degree${\times}2$degree field, the optical system must satisfy the following conditions: 1) The tilt error between detector plane and focal plane should be less than 0.05degree. 2) The decenter error between the primary mirror and the correcting lens system should be less than 1mm. 3) The distance error between the primary mirror and the correcting lens system should be less than 2.3mm. In order to align the optical system accurately, we measured the aberrations of the telescope quantitatively by means of curvature sensing technique. NEOPAT-3 is installed temporary on the roof of the TRAO(Taeduk Radio Astronomy Observatory) main building to normalize system performance and to develop automatic observation.

Space Debris Tracking Coverage Analysis of Spinning Disk for Optical Path Switch of Geochang Laser Tracking System (거창 레이저 추적 시스템의 광 경로 전환을 위한 회전 디스크의 우주쓰레기 레이저 추적 성능 분석)

  • Sung, Ki-Pyoung;Lim, Hyung-Chul;Yu, Sung-Yeol;Choi, Man-Soo;Ryou, Jae-Cheol
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.48 no.5
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    • pp.391-399
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    • 2020
  • KASI (Korea Astronomy and Space Science Institute) has been developing the multipurpose laser tracking system with three functions of satellite laser tracking, adaptive optics and space debris laser tracking for scientific research and national space missions. The space debris laser tracking system provides the distance to space debris without a laser retro-reflector array by using a high power pulse laser, which employs a spinning disk to change the optical path between the transmit and receive beams. The spinning disk causes the collision band which is unable to reflect the returned signal to a detector and then has an effect on the tracking coverage of space debris. This study proposed the mathematical model for tracking coverage by taking into account the various specifications of spinning disk such as disk size, spinning velocity and collision rate between the disk and hole. In addition, the spinning disk specifications were analyzed in terms of tracking coverage and collision band based on the mathematical model to investigate tracking requirements of the Geochang laser tracking system.

Efficiency of Closed Cutting Propagation Affected by Closed Periods, Leaflet Number and Photoperiod in Rose (Rosa hybrida) (밀폐 기간, 소엽수 및 광주기에 따른 장미의 밀폐삽목 번식 효율)

  • Yang, Gyeong Rok;Jung, Hyun Hwan;Park, Ki Young;Song, Kwan Jeong
    • Journal of Bio-Environment Control
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    • v.31 no.3
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    • pp.212-220
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    • 2022
  • When it comes to single node leafy stem cuttings of rose (Rosa hybrida), environmental management such as air temperature, relative humidity, and light affect productivity. In order to investigate the effect of air temperature and relative humidity on the cutting success rate and rooted cuttings quality, a transparent airtight box was used to implement a closed system. We have also tried to find out the most effective photoperiod and the number of leaflets in closed system using artificial light (white LED, 104.0 µmol·m-2·s-1 photosynthetic photon flux density). The first experiment was conducted for a total of 6 weeks under 4 airtight period conditions. The number of roots and longest root length decreased as the airtight period increased. But there were no significant differences in the survival rate, shooting rate, and rooting rate according to airtight periods. In the second experiment the results indicated that survival and shooting rate were significantly affected by the photoperiod (0/24, 2/22, 4/20, 8/16, and 16/8 h), the number of leaflets (0, 2, and 4 leaflets) of the cuttings and their interaction. The survival rate was the highest in the 16-h day length and 4 leaflets. By considering survival rate and shooting rate with energy efficiency, the 8-h day length and 2 or 4 leaflets were judged to be the most effective.

Influence of Light Intensity and Photoperiod on the Growth and Phenol Contents of Hydroponic Basil in Vertical Farms (수직농장에서 광도와 광주기가 수경재배 바질의 생육 및 페놀 함량에 미치는 영향)

  • Sunwoo Kim;Jongseok Park
    • Journal of Bio-Environment Control
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    • v.32 no.4
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    • pp.353-358
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    • 2023
  • This study aimed to investigate the growth and phenol content changes of basil (Ocimum basilicum L.) under five different light intensities and photoperiods, maintaining the same Daily Light Integral (DLI) conditions in a plant factory. Basil seeds were sown on a rockwool medium for four weeks and then transplanted. To maintain a DLI 17mol·m-2·d-1, light intensity and photoperiod were set at 16h-295, 18h-260, 20h-235, 22h-215, and 24h-200μmol·m-2·s-1 and cultivated for four weeks. The harvested results showed that basil plant height, number of lateral branches, and leaf number tended to decrease from the 16h-295 treatment to the 24h-200 treatment. Shoot fresh weight, dry weight, leaf area, leaf width, and leaf length were significantly higher in the 18 h-260 treatment. The total phenolic contents in the 18h-260 treatment was significantly higher by 51.3%, 172.7%, 111%, and 119.7% compared to the 16h-295, 20h-235, 22h-215, and 24h-200 treatments, respectively. Therefore, it is anticipated that cultivating basil under the condition of 18h-260 treatment could yield enhanced growth quality and an increase in total phenolic contents.

Aboveground Biomass and Nutrient Distribution of Korea Pine (Pinus koraiensis) Advance Growth in Deciduous Oak Forests (참나무림내 천연발생한 잣나무 치수의 지상부 현존량 및 양분분포)

  • Ji, Dong-Hun;Byun, Jae-Kyoung;Jeong, Jin-Hyun;Yi, Myong-Jong
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.6
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    • pp.1144-1149
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    • 2011
  • The occurrence of Korean pine (Pinus koraiensis) advance growth has been noticed in the deciduous oak forest being adjacent to the mature Korean pine plantation. the korean pine seedlings in the forest were regenerated naturally from harvested and transported Korean pine seeds and cones by rodents, mainly red squirrels. Mongolian oak (Quercus bariabilis), which formed dominant overstory, had close-to-normal distributions of height, diameter and age classes. Korean pine, which dominated the understory, had the reverse J-shaped height, diameter and age distributions that are expected of a shade tolerant species. Growing in the summer shade of the oak, Korean pine seedling had slow, but steady height growth during the past four decades. Total biomass of Korean pine seedling ranged from $2,835-8,541kg\;ha^{-1}$ and biomass allocation of Korean pine seedling was follows : stem (with bark) > foliage > branches > roots. Korean pine seedlings allocated more biomass to foliage and branches compare to planted Korean pine. The smaller root/shoot ratio of Korean pine seedling in the site is to believed to be result of competition for light. Contents of N and K for Korean pine seedling were greatest in foliage follow by branches, stem ad roots, while content of P was greater in the order of foliage > roots > stem > branches.

Effects on Dry Matter Production and Growth of Zoysia japonica under the Light Environment II. Effect of Dry Matter Production and Growth Construction of Zoysia japonica on a Shade Rate (광환경이 잔디(Zoysia japonica)의 물질생산과 생장에 미치는 영향 II. 차광율이 잔디의 물질생산과 성장해석에 미치는 영향)

  • 도봉현;김광식
    • Asian Journal of Turfgrass Science
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    • v.6 no.2
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    • pp.73-81
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    • 1992
  • This study was designed to estimate on the interaction of the first productivity, light condition and to examine the ecophysiological 'characteristics of Zoysia japonica. Artificial community of Zoysia japonica was analyzed effect of matter production and growth construction under various shading condition. The results summarized this experiment were as follows ; 1. The relative growth rate(RGR) in all experimental plots was high during the growth stage of 20 days after transplanting and then tend to decrease. RGR value in severe shading or short day condition was remarkably decreased from the early growth stage. 2. The net assimilation rate(NAR) was high during the early growth stage after transplanting. and then decreased the growth proceeded. NAR value in the severe shading or short day condition plots was low. 3. The increasing rate of leaf ared ratio(LAR) was high during the early growth stage after transplanting in the control plot and 30% shading plot, but it appeared to be high 40 days after transplanting in the 50% and 70% shading plot. LAR was appeared to be negative correlation against RGR and NAR. 4. The crop growth rate(CGR) in each experimental plot was increased until 50 days after transplanting, and then decreased. After that time, the maximum CGR value appeared in the control plot(1.56g /g, 10days), 60days after transplanting. CGR had positive correlation with LAI. The optimum LAI in the control, 30%, 50%, and 70% shading plots was appeared as 2.10, 1.75, 1.25, and 0.90.

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A Study on Low Power Energy Transfer Circuits of the Non Contact Method by means of Solar Generation (태양광 발전에 의한 비접촉 방식 저 전력 에너지 전송회로에 관한 연구)

  • Hwang, Lark-Hoon;Na, Seung-Kwon;Kim, Jong-Rae;Choi, Gi-Ho;Kim, Jin-Seon
    • Journal of Advanced Navigation Technology
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    • v.18 no.1
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    • pp.35-43
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    • 2014
  • In this paper, it is about to non-contact wireless power transmission according to various conditions of self induction principle between the two planar coils at a transmission unit and a receiving unit based on the theory of wireless power transmission. The experiments are occurred in order to power transfer of noncontact method from designed wireless circuits in the primely coil and secondary coil, and the applying to Half Bridge Resonant converter transmission unit and receiving unit. and that were able to prepared circumstance to calculate of the output voltage and power source. The main power of the inductive coupling the resonant converter at the transmission unit is converted electrical energy using the solar cell module and artificial light source (halogen lamp) as a replace light and received 24 V power supply from solar power was used a input power source for the wireless power transmission device. Experimental results, to received of power is used to illuminate the lighting and to charge the battery in receiving circuit.And the wireless power transmission efficiency measured at the output side of the transmission unit is obtained about 70% to 89% compared to input power of receiving unit.In addition, efficiency were tested through ID verification method and comparing the phase difference between the voltage when foreign substances interfere with wireless power transmission.

Rapid Micmpmpagation of Pimpinella barchycarpa via Somatic Embryogenesis (참나물(Pimpinella barchycarpa)의 체세포 경발생에 의한 식물체 대량증식)

  • Moon, Heung-Kyu;Yoon, Yang;Lee, Seok-Gu
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.2
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    • pp.85-90
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    • 1994
  • Attempts were made to regenerate plants from petiole explane of Forest Pimpinella barchycarpa via repetitive somatic embryogenesis. Effective induction of somatic emb교ogenesis was achieved on both MS and modified $B_{5}\;(mB_{5})$ media containing BA + 2,4-D or BA + 2,4-D + NAA under light condition (16-h photoperiod/day) cultures. The explants exposed to the ligt produced numerous somatic embryos while those kept under the dark did not form any on the same medium. Somatic embryos at different developmental stages were observed to arise within a individual explants. Plantlets could be regenerated on $mB_{5}$ basal medium or $mB_{5}$ containing 0.1 mg/L NAA Secondary adventive embryos were formed on the surface of the somatic embryos. Therefore, repetitive somatic embryogenesis could be achieved by secondary embryogenesis. Although the treatment of 2,4-D or NAA alone was effective in callus formation and growth, but not in induction of somatic embryogenesis. Some explants, cultured on NAA-containing media in darkness, produced only adventive roots. The embryogenic potential was maintained for two years when subcultured to BA and 2,4-D containing media with 5 weeks inteval. Regenerated plantlets were maintained on $mB_{5}$ or MS basal media for 4 to 6 more weeks and transferred to soil of an artificial mixture for acclimation. Most plantlets (more than 97%) survived, and grew without any deformity.

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